Click here to switch to the map view.

The map label for this gene is cwlC

Identifier: 255767407

GI number: 255767407

Start: 1872812

End: 1873579

Strand: Reverse

Name: cwlC

Synonym: BSU17410

Alternate gene names: 255767407

Gene position: 1873579-1872812 (Counterclockwise)

Preceding gene: 255767416

Following gene: 255767404

Centisome position: 44.44

GC content: 46.09

Gene sequence:

>768_bases
ATGGTTAAAATTTTTATTGATCCTGGCCATGGCGGGTCTGATCCAGGCGCAACAGGTAATGGCCTTCAGGAGAAAACGTT
AACCCTGCAAATCGCTTTAGCCTTACGTACGATATTAACTAATGAATATGAAGGCGTTTCTCTGCTGCTGAGCCGGACAA
GCGACCAATATGTCAGCTTAAACGACCGGACAAATGCCGCAAATAACTGGGGAGCAGATTTCTTTTTGTCCATTCACGTT
AATTCCGGGGGAGGCACAGGTTTTGAAAGCTATATTTATCCAGATGTAGGAGCCCCGACGACGACTTATCAATCGACAAT
TCACTCTGAAGTGATACAAGCTGTCGACTTTGCCGATCGCGGCAAAAAAACAGCGAACTTCCACGTCCTAAGGGAGTCGG
CAATGCCTGCCCTCTTGACCGAGAACGGCTTCATTGATACCGTTTCCGATGCAAATAAGCTGAAAACGAGCAGTTTTATT
CAAAGCTTAGCGAGAGGACATGCAAACGGGCTGGAGCAAGCCTTTAACCTTAAAAAGACTTCCAGCTCAGGGTTATATAA
GGTTCAAATCGGCGCATTTAAAGTCAAAGCGAATGCCGACTCGCTCGCAAGTAATGCCGAAGCCAAAGGTTTTGACTCGA
TTGTCCTTTTAAAGGACGGATTATACAAAGTGCAGATTGGCGCATTTTCATCCAAAGACAATGCAGACACCCTCGCTGCC
AGAGCGAAAAATGCCGGCTTTGACGCTATTGTGATCCTAGAATCATAG

Upstream 100 bases:

>100_bases
TTTCTGGATGTGAAAGGGTTATTTCCTCATTTTTCAGTTGAATGATAATCGTACAAGCAGAAGCCGTGTTTTTTCATATC
CTGTAATGAGGTGATGAAAA

Downstream 100 bases:

>100_bases
CCGAGACGGGGACGAGCGTCTCATAAAAAAACCCGGCTCCTCATCGCGAGAAACCGGGTTTTTTTATTCAAGAATATCAA
CAACAAACTGTGACCATGTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MVKIFIDPGHGGSDPGATGNGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSLNDRTNAANNWGADFFLSIHV
NSGGGTGFESYIYPDVGAPTTTYQSTIHSEVIQAVDFADRGKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFI
QSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAA
RAKNAGFDAIVILES

Sequences:

>Translated_255_residues
MVKIFIDPGHGGSDPGATGNGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSLNDRTNAANNWGADFFLSIHV
NSGGGTGFESYIYPDVGAPTTTYQSTIHSEVIQAVDFADRGKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFI
QSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAA
RAKNAGFDAIVILES
>Mature_255_residues
MVKIFIDPGHGGSDPGATGNGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSLNDRTNAANNWGADFFLSIHV
NSGGGTGFESYIYPDVGAPTTTYQSTIHSEVIQAVDFADRGKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFI
QSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAA
RAKNAGFDAIVILES

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze typ

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall. Note=Is associated with the mother cell wall of sporulating cells

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family

Homologues:

Organism=Escherichia coli, GI1788776, Length=221, Percent_Identity=27.6018099547511, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790611, Length=228, Percent_Identity=27.6315789473684, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLC_BACSU (Q06320)

Other databases:

- EMBL:   D14666
- EMBL:   AL009126
- EMBL:   Z68500
- PIR:   A36935
- RefSeq:   NP_389623.2
- PDB:   1X60
- PDBsum:   1X60
- ProteinModelPortal:   Q06320
- SMR:   Q06320
- EnsemblBacteria:   EBBACT00000000598
- GeneID:   940088
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17410
- GenoList:   BSU17410
- GeneTree:   EBGT00070000031903
- HOGENOM:   HBG656726
- ProtClustDB:   CLSK872830
- BioCyc:   BSUB:BSU17410-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002508
- InterPro:   IPR007730
- Gene3D:   G3DSA:3.40.630.40
- Gene3D:   G3DSA:3.30.70.1070
- SMART:   SM00646

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR; SSF110997 Spore_cell-division_bac

EC number: =3.5.1.28

Molecular weight: Translated: 27147; Mature: 27147

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIFIDPGHGGSDPGATGNGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSL
CEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
NDRTNAANNWGADFFLSIHVNSGGGTGFESYIYPDVGAPTTTYQSTIHSEVIQAVDFADR
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
GKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFIQSLARGHANGLEQAFNLKKT
CCCCCEEEEEECCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
SSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAA
CCCCEEEEEEEEEEEECCCHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHHHH
RAKNAGFDAIVILES
HHCCCCCCEEEEEEC
>Mature Secondary Structure
MVKIFIDPGHGGSDPGATGNGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSL
CEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
NDRTNAANNWGADFFLSIHVNSGGGTGFESYIYPDVGAPTTTYQSTIHSEVIQAVDFADR
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
GKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFIQSLARGHANGLEQAFNLKKT
CCCCCEEEEEECCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
SSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAA
CCCCEEEEEEEEEEEECCCHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCCHHHHHH
RAKNAGFDAIVILES
HHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407798; 9384377