Click here to switch to the map view.

The map label for this gene is pksE

Identifier: 255767393

GI number: 255767393

Start: 1786104

End: 1788407

Strand: Direct

Name: pksE

Synonym: BSU17120

Alternate gene names: 255767393

Gene position: 1786104-1788407 (Clockwise)

Preceding gene: 255767392

Following gene: 18677776

Centisome position: 42.37

GC content: 42.84

Gene sequence:

>2304_bases
ATGATTACATATGTCTTTCCAGGGCAAGGCTCCCAGCAAAAGGGGATGGGACAAGGGCTATTTGAACAGTATCAGCATTT
GACAGATCAAGCAGATCAAATATTGGGCTATTCAATAGAGAAGCTATGTACCGAAAAATCATATCTGGATGTAAATCATA
CTGAATACACGCAGCCGGCTTTGTATGTTGTTAATGCACTCAGTTACTTGAAGAGAGTGGAGGAGACCGGCAGAAAACCT
GATTTTGCGGCAGGACACAGCTTAGGAGAATACAATGCGCTGATGGCAGCCGGTGCATTTGATTTTGAAACCGGATTAAG
GCTCGTCAAAAAAAGAGGAGAATTGATGGGACGAATTACAGGGGGCGGAATGGCTGCTGTGATCGGCCTGAGTAAGGAAC
AAGTTACAGCCGTTTTAGAAGAGCACCGTCTTTATGACATTGATGTGGCAAATGAAAATACGCCCCAGCAAATTGTGATT
TCTGGCCCGAAAAAAGAGATTGAAAAAGCTCGGGCTGTTTTTGAAAACACCAAGGATGTAAAGCTGTTTCATCCATTAAA
TGTGAGCGGAGCTTTTCATTCCAGATATATGAATGAAGCCAAACAGGTATTTAAGCAATATATTGACTCATTCCAGTTTG
CACCTTTGGCGATCCCGGTTATCTCCAATGTCTACGCAGAGCCATACCATCAAGACAGATTAAAAGATACATTATCTGAA
CAAATGGACAACACAGTGAAATGGACGGACAGCATTCGTTTTCTGATGGGAAGAGGAGAAATGGAGTTTGCAGAGATTGG
GCCTGGAACTGTTTTAACCGGTCTCATTCATCGGATTAAAAATGAAGCTGAACCATTGACATATATACCGAAAAAGAACC
CCGCGATCAGTGCGCATCTAAAGGAGCAACGCAATGTTCAAGCCGGGATTACGGCAGAATCTTTAGGAAGTGCTGAATTT
AAACAAGATTATCATCTTACATATGCTTATCTTGCAGGAGGAATGTACCGCGGCATTGCTTCGAAAGAAATGGTAGTAAA
GCTGTCAAGAGCGGGAATGATGGGGTTCTTTGGCACAGGAGGTCTGAGCCTGAAAGAGGTTGAAGATGCGATCCATGCTA
TACAAGGTGAATTAGGAAAAGGACAAGCCTACGGCATCAATCTGGTCCACAATATGAAACATACTGAATCAGAAGAAAAA
ATGATCGATCTTTTATTGAGGAATCAAGTGAGCATTGTGGAGGCTTCAGCATTTTTAAGTGTCACTCCTGTATTAGTAAG
GTATCGCGCAAAAGGAGTGAAACGAAATCAAAATGGTGACGTTATATGCTCAAATCGTCTCATAGCAAAAATATCACGGC
CGGAAGTTGCAGAATCATTTCTGAGTCCTGCGCCTGAGAATATGCTGCAAAAGCTTTTAGGAGAAAATAAAATCACTATG
AACGAAGCTGAGTTGTTGCGGTGCATTCCGATGGCTGACGATATTTGTGTAGAAGCTGATTCAGGCGGTCATACAGATGG
CGGTGTGGCATACAGCCTGATGCCGGCGATGACATCTTTAAGAGATGAAATGATGAAAAAATATCAATACCGGAAAAAAA
TAAGAGTTGGTGCAGCTGGAGGAATTGGAACACCTGAGGCAGCGATGGCGGCTTTTATGCTAGGGGCTGATTTTATTCTA
ACAGGCTCAATCAATCAATGTACGGTTGAGGCGGCAACAAGTGATAAAGTAAAGGATTTGCTTCAGCAGATGAATGTACA
AGATACAGCCTATGCGCCTGCAGGTGATATGTTTGAATCGGGCAGTAAAGTGCAGGTGCTAAAGAAAGGCGTGTTCTTTC
CCGCACGGGCAAACAAGTTATATGAACTGTATCAGCGGTATGGGTCTATACGTGAGCTGGATGCAAAGATGCTGGCCCAG
CTTGAGGAGAAATACTTTAAACGCAGTATAGAAGACATATATAAAGATATTGCATTGCATTATCCCGCGGCAGACATTGA
GAAGGCTGAACAAAACCCGAAGCATAAAATGGCTTTGATTTTCCGATGGTACTTCAGATACAGTTCAAAATTGGCAATCA
GCGGAAGCGAACATTCGAAGGTCGATTATCAAATTCACTGCGGTCCCGCGCTGGGAGCCTTTAATCAATGGGTTAAAGGG
TCGCAATTAGAAAATTGGCGAAACCGCCATGTGGATGAAATCGGGAAAAAATTAATGACAGAAACGGCAGTATTGCTTCA
TGAACGGATGCAATCGATGTATCAGCCGTCGCATGAAACAGACAACATAAAAATAAAAGTTTGA

Upstream 100 bases:

>100_bases
CAGCTGACCGTTGCTGCTCAATCATAACACCATTTCATCAGGAGCTGAAAAATCTGAATACGGTTGAGTATTTCCGTACA
CCAGAAAGGAAGTTTACAAG

Downstream 100 bases:

>100_bases
ATATTATGAAAATATACCTATAAAAATCATTAAAATAATCCCTTGAAGGAGAGAGCGCATTATGGATAAACAGAGAATCT
TTGAAGTATTAATCACCAAT

Product: polyketide synthesis protein

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT

Number of amino acids: Translated: 767; Mature: 767

Protein sequence:

>767_residues
MITYVFPGQGSQQKGMGQGLFEQYQHLTDQADQILGYSIEKLCTEKSYLDVNHTEYTQPALYVVNALSYLKRVEETGRKP
DFAAGHSLGEYNALMAAGAFDFETGLRLVKKRGELMGRITGGGMAAVIGLSKEQVTAVLEEHRLYDIDVANENTPQQIVI
SGPKKEIEKARAVFENTKDVKLFHPLNVSGAFHSRYMNEAKQVFKQYIDSFQFAPLAIPVISNVYAEPYHQDRLKDTLSE
QMDNTVKWTDSIRFLMGRGEMEFAEIGPGTVLTGLIHRIKNEAEPLTYIPKKNPAISAHLKEQRNVQAGITAESLGSAEF
KQDYHLTYAYLAGGMYRGIASKEMVVKLSRAGMMGFFGTGGLSLKEVEDAIHAIQGELGKGQAYGINLVHNMKHTESEEK
MIDLLLRNQVSIVEASAFLSVTPVLVRYRAKGVKRNQNGDVICSNRLIAKISRPEVAESFLSPAPENMLQKLLGENKITM
NEAELLRCIPMADDICVEADSGGHTDGGVAYSLMPAMTSLRDEMMKKYQYRKKIRVGAAGGIGTPEAAMAAFMLGADFIL
TGSINQCTVEAATSDKVKDLLQQMNVQDTAYAPAGDMFESGSKVQVLKKGVFFPARANKLYELYQRYGSIRELDAKMLAQ
LEEKYFKRSIEDIYKDIALHYPAADIEKAEQNPKHKMALIFRWYFRYSSKLAISGSEHSKVDYQIHCGPALGAFNQWVKG
SQLENWRNRHVDEIGKKLMTETAVLLHERMQSMYQPSHETDNIKIKV

Sequences:

>Translated_767_residues
MITYVFPGQGSQQKGMGQGLFEQYQHLTDQADQILGYSIEKLCTEKSYLDVNHTEYTQPALYVVNALSYLKRVEETGRKP
DFAAGHSLGEYNALMAAGAFDFETGLRLVKKRGELMGRITGGGMAAVIGLSKEQVTAVLEEHRLYDIDVANENTPQQIVI
SGPKKEIEKARAVFENTKDVKLFHPLNVSGAFHSRYMNEAKQVFKQYIDSFQFAPLAIPVISNVYAEPYHQDRLKDTLSE
QMDNTVKWTDSIRFLMGRGEMEFAEIGPGTVLTGLIHRIKNEAEPLTYIPKKNPAISAHLKEQRNVQAGITAESLGSAEF
KQDYHLTYAYLAGGMYRGIASKEMVVKLSRAGMMGFFGTGGLSLKEVEDAIHAIQGELGKGQAYGINLVHNMKHTESEEK
MIDLLLRNQVSIVEASAFLSVTPVLVRYRAKGVKRNQNGDVICSNRLIAKISRPEVAESFLSPAPENMLQKLLGENKITM
NEAELLRCIPMADDICVEADSGGHTDGGVAYSLMPAMTSLRDEMMKKYQYRKKIRVGAAGGIGTPEAAMAAFMLGADFIL
TGSINQCTVEAATSDKVKDLLQQMNVQDTAYAPAGDMFESGSKVQVLKKGVFFPARANKLYELYQRYGSIRELDAKMLAQ
LEEKYFKRSIEDIYKDIALHYPAADIEKAEQNPKHKMALIFRWYFRYSSKLAISGSEHSKVDYQIHCGPALGAFNQWVKG
SQLENWRNRHVDEIGKKLMTETAVLLHERMQSMYQPSHETDNIKIKV
>Mature_767_residues
MITYVFPGQGSQQKGMGQGLFEQYQHLTDQADQILGYSIEKLCTEKSYLDVNHTEYTQPALYVVNALSYLKRVEETGRKP
DFAAGHSLGEYNALMAAGAFDFETGLRLVKKRGELMGRITGGGMAAVIGLSKEQVTAVLEEHRLYDIDVANENTPQQIVI
SGPKKEIEKARAVFENTKDVKLFHPLNVSGAFHSRYMNEAKQVFKQYIDSFQFAPLAIPVISNVYAEPYHQDRLKDTLSE
QMDNTVKWTDSIRFLMGRGEMEFAEIGPGTVLTGLIHRIKNEAEPLTYIPKKNPAISAHLKEQRNVQAGITAESLGSAEF
KQDYHLTYAYLAGGMYRGIASKEMVVKLSRAGMMGFFGTGGLSLKEVEDAIHAIQGELGKGQAYGINLVHNMKHTESEEK
MIDLLLRNQVSIVEASAFLSVTPVLVRYRAKGVKRNQNGDVICSNRLIAKISRPEVAESFLSPAPENMLQKLLGENKITM
NEAELLRCIPMADDICVEADSGGHTDGGVAYSLMPAMTSLRDEMMKKYQYRKKIRVGAAGGIGTPEAAMAAFMLGADFIL
TGSINQCTVEAATSDKVKDLLQQMNVQDTAYAPAGDMFESGSKVQVLKKGVFFPARANKLYELYQRYGSIRELDAKMLAQ
LEEKYFKRSIEDIYKDIALHYPAADIEKAEQNPKHKMALIFRWYFRYSSKLAISGSEHSKVDYQIHCGPALGAFNQWVKG
SQLENWRNRHVDEIGKKLMTETAVLLHERMQSMYQPSHETDNIKIKV

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family

Homologues:

Organism=Homo sapiens, GI51243059, Length=309, Percent_Identity=30.42071197411, Blast_Score=110, Evalue=7e-24,
Organism=Escherichia coli, GI1787334, Length=284, Percent_Identity=34.8591549295775, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550940, Length=295, Percent_Identity=28.135593220339, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71984332, Length=304, Percent_Identity=27.9605263157895, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24665753, Length=304, Percent_Identity=28.9473684210526, Blast_Score=103, Evalue=6e-22,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PKSE_BACSU (O34787)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_389593.3
- HSSP:   P0AAI9
- ProteinModelPortal:   O34787
- EnsemblBacteria:   EBBACT00000000460
- GeneID:   939997
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17120
- GenoList:   BSU17120
- GeneTree:   EBGT00050000000472
- HOGENOM:   HBG337972
- ProtClustDB:   CLSK928131
- BioCyc:   BSUB:BSU17120-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179
- Gene3D:   G3DSA:3.40.366.10
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00128
- TIGRFAMs:   TIGR02814

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD; SSF52151 Acyl_Trfase/lysoPlipase; SSF55048 Malonyl_transacylase_ACP-bd

EC number: =2.3.1.39

Molecular weight: Translated: 85744; Mature: 85744

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: ACT_SITE 87-87 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITYVFPGQGSQQKGMGQGLFEQYQHLTDQADQILGYSIEKLCTEKSYLDVNHTEYTQPA
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LYVVNALSYLKRVEETGRKPDFAAGHSLGEYNALMAAGAFDFETGLRLVKKRGELMGRIT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEC
GGGMAAVIGLSKEQVTAVLEEHRLYDIDVANENTPQQIVISGPKKEIEKARAVFENTKDV
CCCEEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHCCCCCE
KLFHPLNVSGAFHSRYMNEAKQVFKQYIDSFQFAPLAIPVISNVYAEPYHQDRLKDTLSE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
QMDNTVKWTDSIRFLMGRGEMEFAEIGPGTVLTGLIHRIKNEAEPLTYIPKKNPAISAHL
HHCCCEEHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
KEQRNVQAGITAESLGSAEFKQDYHLTYAYLAGGMYRGIASKEMVVKLSRAGMMGFFGTG
HHHCCCCCCCCHHHCCCHHHHHCHHHHHHHHHCHHHHCCCCHHHHHHHHHCCCEEEECCC
GLSLKEVEDAIHAIQGELGKGQAYGINLVHNMKHTESEEKMIDLLLRNQVSIVEASAFLS
CCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
VTPVLVRYRAKGVKRNQNGDVICSNRLIAKISRPEVAESFLSPAPENMLQKLLGENKITM
HHHHHHHHHHHCCCCCCCCCEEECCCHHEECCCCHHHHHHHCCCHHHHHHHHHCCCCEEE
NEAELLRCIPMADDICVEADSGGHTDGGVAYSLMPAMTSLRDEMMKKYQYRKKIRVGAAG
CHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GIGTPEAAMAAFMLGADFILTGSINQCTVEAATSDKVKDLLQQMNVQDTAYAPAGDMFES
CCCCHHHHHHHHHHCCCEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHC
GSKVQVLKKGVFFPARANKLYELYQRYGSIRELDAKMLAQLEEKYFKRSIEDIYKDIALH
CCHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPAADIEKAEQNPKHKMALIFRWYFRYSSKLAISGSEHSKVDYQIHCGPALGAFNQWVKG
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHCC
SQLENWRNRHVDEIGKKLMTETAVLLHERMQSMYQPSHETDNIKIKV
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure
MITYVFPGQGSQQKGMGQGLFEQYQHLTDQADQILGYSIEKLCTEKSYLDVNHTEYTQPA
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LYVVNALSYLKRVEETGRKPDFAAGHSLGEYNALMAAGAFDFETGLRLVKKRGELMGRIT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEC
GGGMAAVIGLSKEQVTAVLEEHRLYDIDVANENTPQQIVISGPKKEIEKARAVFENTKDV
CCCEEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHCCCCCE
KLFHPLNVSGAFHSRYMNEAKQVFKQYIDSFQFAPLAIPVISNVYAEPYHQDRLKDTLSE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
QMDNTVKWTDSIRFLMGRGEMEFAEIGPGTVLTGLIHRIKNEAEPLTYIPKKNPAISAHL
HHCCCEEHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
KEQRNVQAGITAESLGSAEFKQDYHLTYAYLAGGMYRGIASKEMVVKLSRAGMMGFFGTG
HHHCCCCCCCCHHHCCCHHHHHCHHHHHHHHHCHHHHCCCCHHHHHHHHHCCCEEEECCC
GLSLKEVEDAIHAIQGELGKGQAYGINLVHNMKHTESEEKMIDLLLRNQVSIVEASAFLS
CCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
VTPVLVRYRAKGVKRNQNGDVICSNRLIAKISRPEVAESFLSPAPENMLQKLLGENKITM
HHHHHHHHHHHCCCCCCCCCEEECCCHHEECCCCHHHHHHHCCCHHHHHHHHHCCCCEEE
NEAELLRCIPMADDICVEADSGGHTDGGVAYSLMPAMTSLRDEMMKKYQYRKKIRVGAAG
CHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GIGTPEAAMAAFMLGADFILTGSINQCTVEAATSDKVKDLLQQMNVQDTAYAPAGDMFES
CCCCHHHHHHHHHHCCCEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHC
GSKVQVLKKGVFFPARANKLYELYQRYGSIRELDAKMLAQLEEKYFKRSIEDIYKDIALH
CCHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPAADIEKAEQNPKHKMALIFRWYFRYSSKLAISGSEHSKVDYQIHCGPALGAFNQWVKG
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHCC
SQLENWRNRHVDEIGKKLMTETAVLLHERMQSMYQPSHETDNIKIKV
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377