Click here to switch to the map view.

The map label for this gene is spoIIIE

Identifier: 255767381

GI number: 255767381

Start: 1752272

End: 1754641

Strand: Direct

Name: spoIIIE

Synonym: BSU16800

Alternate gene names: 255767381

Gene position: 1752272-1754641 (Clockwise)

Preceding gene: 255767380

Following gene: 16078744

Centisome position: 41.57

GC content: 44.98

Gene sequence:

>2370_bases
ATGAGTGTGGCAAAGAAAAAACGAAAATCAAGAAAAAAACAGGCGAAACAGCTCAATATAAAATACGAGCTCAACGGATT
GCTGTGTATAGCCATTTCAATTATCGCAATCTTGCAGCTGGGGGTAGTCGGGCAAACGTTTATCTATTTGTTCCGCTTTT
TTGCTGGAGAGTGGTTTATTTTATGCTTGTTAGGTTTATTGGTGTTAGGAGTCTCACTGTTTTGGAAGAAAAAAACCCCA
AGCCTTTTAACGAGACGAAAGGCCGGGTTGTACTGTATTATCGCAAGCATATTGCTGCTTTCTCACGTGCAGCTATTTAA
AAATTTGACGCACAAAGGGTCTATTGAGTCTGCAAGCGTGGTGCGCAATACGTGGGAATTGTTTTTAATGGACATGAATG
GCAGCTCTGCTTCACCTGATTTAGGCGGAGGGATGATCGGTGCGCTGCTGTTTGCGGCTTCACACTTTTTGTTTGCGTCG
ACTGGTTCTCAGATCATGGCAATTGTCATGATTTTGATCGGAATGATTTTAGTAACAGGACGCTCGCTGCAAGAAACGCT
AAAAAAGTGGATGAGCCCGATTGGACGTTTTATAAAAGAACAATGGTTAGCATTTATTGATGACATGAAATCCTTCAAAT
CAAATATGCAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAACCGGCCCGCAAAAAACAGCAAATGGAACCG
GAGCCTCCTGATGAAGAGGGGGATTATGAAACAGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCTGA
TCGTAATGAAGAGGAAGAGTCTCCAGTTATAGAAAAGCGCGCCGAACCTGTGTCGAAGCCGCTTCAGGACATCCAACCGG
AGACAGGTGATCAGGAAACTGTTTCTGCTCCTCCTATGACCTTTACAGAGCTCGAAAATAAGGATTACGAGATGCCGTCA
CTGGATTTGCTGGCAGATCCGAAGCATACCGGCCAGCAGGCTGATAAAAAGAATATTTATGAAAATGCGAGAAAGCTTGA
ACGCACATTCCAAAGCTTTGGCGTAAAGGCGAAAGTCACACAGGTTCATCTCGGACCGGCCGTAACAAAATATGAAGTAT
ATCCTGATGTCGGAGTGAAGGTTAGCAAAATTGTCAATTTAAGCGATGATTTAGCGCTTGCGCTTGCTGCGAAGGATATC
AGGATCGAAGCGCCTATACCAGGCAAATCAGCAATCGGAATTGAAGTTCCAAACGCAGAAGTGGCAATGGTTTCGCTGAA
AGAAGTGCTGGAGTCTAAATTGAATGACAGACCGGATGCGAAGCTGTTAATTGGACTCGGCCGCAATATTTCAGGTGAAG
CTGTATTGGCTGAGTTAAACAAGATGCCCCACCTTTTGGTCGCAGGCGCAACCGGTAGCGGGAAAAGTGTCTGTGTCAAC
GGCATTATTACAAGTATTTTAATGCGGGCGAAACCGCATGAAGTGAAAATGATGATGATCGATCCTAAAATGGTAGAGCT
AAATGTCTACAACGGGATTCCGCATTTGCTTGCTCCTGTTGTAACCGATCCGAAAAAAGCGTCGCAGGCTTTGAAAAAGG
TTGTCAATGAAATGGAGCGGCGGTACGAATTATTTTCTCATACGGGTACAAGAAATATTGAAGGATACAATGATTATATA
AAACGCGCCAACAATGAAGAAGGAGCGAAACAGCCAGAGCTGCCTTATATTGTTGTCATTGTGGACGAGCTAGCCGATCT
CATGATGGTCGCTTCATCTGATGTGGAAGATTCGATTACGAGATTGTCCCAAATGGCGCGGGCTGCCGGCATCCATCTTA
TTATTGCGACACAGCGGCCATCGGTGGATGTTATCACAGGGGTGATCAAAGCGAACATTCCGTCACGAATCGCGTTCAGC
GTATCTTCACAGACGGATTCAAGGACCATTCTTGACATGGGTGGCGCTGAGAAGCTTCTTGGCCGCGGCGATATGCTGTT
TTTACCTGTAGGGGCTAACAAACCTGTCCGTGTGCAGGGAGCATTTTTGTCGGATGACGAAGTGGAGAAGGTCGTCGACC
ATGTGATTACTCAGCAGAAAGCGCAATATCAAGAAGAAATGATTCCTGAGGAGACGACGGAAACTCATTCCGAGGTTACT
GATGAACTTTATGATGAAGCTGTTGAATTAATCGTCGGCATGCAGACGGCATCTGTTTCAATGCTGCAAAGAAGATTCAG
AATTGGCTATACGAGAGCGGCGCGCCTGATTGACGCAATGGAGGAACGCGGTGTTGTCGGACCGTATGAAGGCAGCAAAC
CTAGGGAAGTATTATTATCAAAAGAGAAATATGATGAGCTCTCTTCTTAA

Upstream 100 bases:

>100_bases
AAATATGCTATAATAGGGGAATCCTACAGAAAAAACGCAGCCTGTAAACGGCTGCTTTCTTCATTATGTATGAACTTACA
TTTTTTAACGCAGTAAGGTG

Downstream 100 bases:

>100_bases
TGAAGGGAGTTCCGCTTTCTATAGTTGTCAATAATGATATTATGTAAACTGATCACCTTTCTATTTATTTTTTCGACAAA
ACATGATATAGTTGTCCCAA

Product: spore DNA translocase

Products: NA

Alternate protein names: DNA translocase SpoIIIE; Stage III sporulation protein E [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MSVAKKKRKSRKKQAKQLNIKYELNGLLCIAISIIAILQLGVVGQTFIYLFRFFAGEWFILCLLGLLVLGVSLFWKKKTP
SLLTRRKAGLYCIIASILLLSHVQLFKNLTHKGSIESASVVRNTWELFLMDMNGSSASPDLGGGMIGALLFAASHFLFAS
TGSQIMAIVMILIGMILVTGRSLQETLKKWMSPIGRFIKEQWLAFIDDMKSFKSNMQSSKKTKAPSKKQKPARKKQQMEP
EPPDEEGDYETVSPLIHSEPIISSFSDRNEEEESPVIEKRAEPVSKPLQDIQPETGDQETVSAPPMTFTELENKDYEMPS
LDLLADPKHTGQQADKKNIYENARKLERTFQSFGVKAKVTQVHLGPAVTKYEVYPDVGVKVSKIVNLSDDLALALAAKDI
RIEAPIPGKSAIGIEVPNAEVAMVSLKEVLESKLNDRPDAKLLIGLGRNISGEAVLAELNKMPHLLVAGATGSGKSVCVN
GIITSILMRAKPHEVKMMMIDPKMVELNVYNGIPHLLAPVVTDPKKASQALKKVVNEMERRYELFSHTGTRNIEGYNDYI
KRANNEEGAKQPELPYIVVIVDELADLMMVASSDVEDSITRLSQMARAAGIHLIIATQRPSVDVITGVIKANIPSRIAFS
VSSQTDSRTILDMGGAEKLLGRGDMLFLPVGANKPVRVQGAFLSDDEVEKVVDHVITQQKAQYQEEMIPEETTETHSEVT
DELYDEAVELIVGMQTASVSMLQRRFRIGYTRAARLIDAMEERGVVGPYEGSKPREVLLSKEKYDELSS

Sequences:

>Translated_789_residues
MSVAKKKRKSRKKQAKQLNIKYELNGLLCIAISIIAILQLGVVGQTFIYLFRFFAGEWFILCLLGLLVLGVSLFWKKKTP
SLLTRRKAGLYCIIASILLLSHVQLFKNLTHKGSIESASVVRNTWELFLMDMNGSSASPDLGGGMIGALLFAASHFLFAS
TGSQIMAIVMILIGMILVTGRSLQETLKKWMSPIGRFIKEQWLAFIDDMKSFKSNMQSSKKTKAPSKKQKPARKKQQMEP
EPPDEEGDYETVSPLIHSEPIISSFSDRNEEEESPVIEKRAEPVSKPLQDIQPETGDQETVSAPPMTFTELENKDYEMPS
LDLLADPKHTGQQADKKNIYENARKLERTFQSFGVKAKVTQVHLGPAVTKYEVYPDVGVKVSKIVNLSDDLALALAAKDI
RIEAPIPGKSAIGIEVPNAEVAMVSLKEVLESKLNDRPDAKLLIGLGRNISGEAVLAELNKMPHLLVAGATGSGKSVCVN
GIITSILMRAKPHEVKMMMIDPKMVELNVYNGIPHLLAPVVTDPKKASQALKKVVNEMERRYELFSHTGTRNIEGYNDYI
KRANNEEGAKQPELPYIVVIVDELADLMMVASSDVEDSITRLSQMARAAGIHLIIATQRPSVDVITGVIKANIPSRIAFS
VSSQTDSRTILDMGGAEKLLGRGDMLFLPVGANKPVRVQGAFLSDDEVEKVVDHVITQQKAQYQEEMIPEETTETHSEVT
DELYDEAVELIVGMQTASVSMLQRRFRIGYTRAARLIDAMEERGVVGPYEGSKPREVLLSKEKYDELSS
>Mature_788_residues
SVAKKKRKSRKKQAKQLNIKYELNGLLCIAISIIAILQLGVVGQTFIYLFRFFAGEWFILCLLGLLVLGVSLFWKKKTPS
LLTRRKAGLYCIIASILLLSHVQLFKNLTHKGSIESASVVRNTWELFLMDMNGSSASPDLGGGMIGALLFAASHFLFAST
GSQIMAIVMILIGMILVTGRSLQETLKKWMSPIGRFIKEQWLAFIDDMKSFKSNMQSSKKTKAPSKKQKPARKKQQMEPE
PPDEEGDYETVSPLIHSEPIISSFSDRNEEEESPVIEKRAEPVSKPLQDIQPETGDQETVSAPPMTFTELENKDYEMPSL
DLLADPKHTGQQADKKNIYENARKLERTFQSFGVKAKVTQVHLGPAVTKYEVYPDVGVKVSKIVNLSDDLALALAAKDIR
IEAPIPGKSAIGIEVPNAEVAMVSLKEVLESKLNDRPDAKLLIGLGRNISGEAVLAELNKMPHLLVAGATGSGKSVCVNG
IITSILMRAKPHEVKMMMIDPKMVELNVYNGIPHLLAPVVTDPKKASQALKKVVNEMERRYELFSHTGTRNIEGYNDYIK
RANNEEGAKQPELPYIVVIVDELADLMMVASSDVEDSITRLSQMARAAGIHLIIATQRPSVDVITGVIKANIPSRIAFSV
SSQTDSRTILDMGGAEKLLGRGDMLFLPVGANKPVRVQGAFLSDDEVEKVVDHVITQQKAQYQEEMIPEETTETHSEVTD
ELYDEAVELIVGMQTASVSMLQRRFRIGYTRAARLIDAMEERGVVGPYEGSKPREVLLSKEKYDELSS

Specific function: DNA motor protein, which is both required to move DNA out of the region of the septum during cell division and for the septum formation. Tracks DNA in an ATP-dependent manner by generating positive supercoils in front of it and negative supercoils behind

COG id: COG1674

COG function: function code D; DNA segregation ATPase FtsK/SpoIIIE and related proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Note=Located at the septum. The large C-terminal part of the protein is cytoplasmic (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FtsK domain [H]

Homologues:

Organism=Escherichia coli, GI1787117, Length=485, Percent_Identity=49.0721649484536, Blast_Score=430, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR018541
- InterPro:   IPR002543 [H]

Pfam domain/function: PF09397 Ftsk_gamma; PF01580 FtsK_SpoIIIE [H]

EC number: NA

Molecular weight: Translated: 87369; Mature: 87237

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50901 FTSK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAKKKRKSRKKQAKQLNIKYELNGLLCIAISIIAILQLGVVGQTFIYLFRFFAGEWFI
CCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCLLGLLVLGVSLFWKKKTPSLLTRRKAGLYCIIASILLLSHVQLFKNLTHKGSIESASV
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VRNTWELFLMDMNGSSASPDLGGGMIGALLFAASHFLFASTGSQIMAIVMILIGMILVTG
HHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
RSLQETLKKWMSPIGRFIKEQWLAFIDDMKSFKSNMQSSKKTKAPSKKQKPARKKQQMEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHCCCC
EPPDEEGDYETVSPLIHSEPIISSFSDRNEEEESPVIEKRAEPVSKPLQDIQPETGDQET
CCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCCCC
VSAPPMTFTELENKDYEMPSLDLLADPKHTGQQADKKNIYENARKLERTFQSFGVKAKVT
CCCCCCCHHHCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
QVHLGPAVTKYEVYPDVGVKVSKIVNLSDDLALALAAKDIRIEAPIPGKSAIGIEVPNAE
EEECCCCEEEEEECCCCCCCHHHHHCCCCHHHHEEEECCEEEECCCCCCCEEEEECCCCH
VAMVSLKEVLESKLNDRPDAKLLIGLGRNISGEAVLAELNKMPHLLVAGATGSGKSVCVN
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHH
GIITSILMRAKPHEVKMMMIDPKMVELNVYNGIPHLLAPVVTDPKKASQALKKVVNEMER
HHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
RYELFSHTGTRNIEGYNDYIKRANNEEGAKQPELPYIVVIVDELADLMMVASSDVEDSIT
HHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCHHHHHH
RLSQMARAAGIHLIIATQRPSVDVITGVIKANIPSRIAFSVSSQTDSRTILDMGGAEKLL
HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCHHHHH
GRGDMLFLPVGANKPVRVQGAFLSDDEVEKVVDHVITQQKAQYQEEMIPEETTETHSEVT
CCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DELYDEAVELIVGMQTASVSMLQRRFRIGYTRAARLIDAMEERGVVGPYEGSKPREVLLS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHC
KEKYDELSS
HHHHHCCCC
>Mature Secondary Structure 
SVAKKKRKSRKKQAKQLNIKYELNGLLCIAISIIAILQLGVVGQTFIYLFRFFAGEWFI
CCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCLLGLLVLGVSLFWKKKTPSLLTRRKAGLYCIIASILLLSHVQLFKNLTHKGSIESASV
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VRNTWELFLMDMNGSSASPDLGGGMIGALLFAASHFLFASTGSQIMAIVMILIGMILVTG
HHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
RSLQETLKKWMSPIGRFIKEQWLAFIDDMKSFKSNMQSSKKTKAPSKKQKPARKKQQMEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHCCCC
EPPDEEGDYETVSPLIHSEPIISSFSDRNEEEESPVIEKRAEPVSKPLQDIQPETGDQET
CCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCCCC
VSAPPMTFTELENKDYEMPSLDLLADPKHTGQQADKKNIYENARKLERTFQSFGVKAKVT
CCCCCCCHHHCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
QVHLGPAVTKYEVYPDVGVKVSKIVNLSDDLALALAAKDIRIEAPIPGKSAIGIEVPNAE
EEECCCCEEEEEECCCCCCCHHHHHCCCCHHHHEEEECCEEEECCCCCCCEEEEECCCCH
VAMVSLKEVLESKLNDRPDAKLLIGLGRNISGEAVLAELNKMPHLLVAGATGSGKSVCVN
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHH
GIITSILMRAKPHEVKMMMIDPKMVELNVYNGIPHLLAPVVTDPKKASQALKKVVNEMER
HHHHHHHHHCCCCCEEEEEECCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
RYELFSHTGTRNIEGYNDYIKRANNEEGAKQPELPYIVVIVDELADLMMVASSDVEDSIT
HHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCHHHHHH
RLSQMARAAGIHLIIATQRPSVDVITGVIKANIPSRIAFSVSSQTDSRTILDMGGAEKLL
HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCHHHHH
GRGDMLFLPVGANKPVRVQGAFLSDDEVEKVVDHVITQQKAQYQEEMIPEETTETHSEVT
CCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DELYDEAVELIVGMQTASVSMLQRRFRIGYTRAARLIDAMEERGVVGPYEGSKPREVLLS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHC
KEKYDELSS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3129532; 2507870; 9384377; 8160014; 10588743; 11062134; 11778051 [H]