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The map label for this gene is cheB

Identifier: 255767373

GI number: 255767373

Start: 1711736

End: 1712809

Strand: Direct

Name: cheB

Synonym: BSU16420

Alternate gene names: 255767373

Gene position: 1711736-1712809 (Clockwise)

Preceding gene: 16078704

Following gene: 255767374

Centisome position: 40.6

GC content: 44.41

Gene sequence:

>1074_bases
TTGATTCGTGTGCTTGTAGTTGATGATTCAGCTTTTATGAGAAAAATGATAAGTGATTTTCTGACCGAAGAAAAGCAGAT
AGAAGTAATCGGAACTGCGAGAAACGGAGAAGAAGCGCTTAAGAAGATTGAATTATTAAAACCGGATGTTATTACTCTTG
ATGTTGAAATGCCGGTCATGAATGGGACAGATACGCTCCGCAAAATTATTGAGATTTATAACTTGCCGGTCATTATGGTG
TCTAGCCAGACCGAGAAAGGTAAAGAATGCACAATTAATTGTTTGGAGATCGGTGCCTTTGACTTTATCACAAAGCCATC
AGGCTCAATATCTCTGGATCTATACAAAATTAAAGAACAACTAGTTGAGCGTGTCGTCGCGGCAGGACTTTCCGGCAAGC
GGAAACGTCCTGTTTCCCAGACAGTTCGGCCCGAACCTATTGTGCGTGCTGTCGTTAAGCCTGAATTAAGCAAACCGAAA
CCTGGCACGGGCAGACAAATTGTGTGTATCGGCACATCAACAGGCGGTCCACGGGCGTTACAAAAGGTGATACCGAAGCT
CCCTAAGGATTTGAACGCACCAGTGGTTGTCGTTCAGCACATGCCTGAAGGGTTTACTGCTTCTTTGGCTGATCGGCTGA
ATCATTTATCTGACATTCAGGTAAAAGAAGCAAAAGATGGTGAAGCAGCTCTTAACGGCTGTGTTTACATAGCACCGGGC
GGAAAAAACATATCCGTTATTAAAAACAGCGAAGGACTCCAGGTTGTGCTTGATAACCATGACACACCAAGCCGTCATAA
ACCATCTGCTGATTATTTATTCCGTTCTGTCGGGAAACTGACAGATTATGAAAAAGTAGCGGTTATCATGACCGGAATGG
GCAGTGACGGCACTGCTGGACTAAAGGACATGCTAACAGCCGGCAATGTGAAAGCCATTGCGGAGTCTGAAGAATCTTGT
GTCGTATACGGGATGCCAAAAGCTGCTGTTAAAGCGGGTCTCATTCATGAGATTAAACATGTGGAAGATATCGCAGCATC
CATCACAAGCTGTGTGAAAAAAGAGAGGGTGTGA

Upstream 100 bases:

>100_bases
TTAGCGGACGCCTTGTTTGAAAGAGAAGAAACGAGACACAAAGAAGACAAACAGACATTTATTGAGAAATTATCTTCTTT
TTTAATGAGGAGGGCTTAAT

Downstream 100 bases:

>100_bases
TTCAAATGGATATGAATCAGTATTTAGATGTCTTTATTGATGAGAGTAAAGAACATTTACAAACATGTAATGAAAAGCTT
CTTCTTTTAGAGAAAGACCC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MIRVLVVDDSAFMRKMISDFLTEEKQIEVIGTARNGEEALKKIELLKPDVITLDVEMPVMNGTDTLRKIIEIYNLPVIMV
SSQTEKGKECTINCLEIGAFDFITKPSGSISLDLYKIKEQLVERVVAAGLSGKRKRPVSQTVRPEPIVRAVVKPELSKPK
PGTGRQIVCIGTSTGGPRALQKVIPKLPKDLNAPVVVVQHMPEGFTASLADRLNHLSDIQVKEAKDGEAALNGCVYIAPG
GKNISVIKNSEGLQVVLDNHDTPSRHKPSADYLFRSVGKLTDYEKVAVIMTGMGSDGTAGLKDMLTAGNVKAIAESEESC
VVYGMPKAAVKAGLIHEIKHVEDIAASITSCVKKERV

Sequences:

>Translated_357_residues
MIRVLVVDDSAFMRKMISDFLTEEKQIEVIGTARNGEEALKKIELLKPDVITLDVEMPVMNGTDTLRKIIEIYNLPVIMV
SSQTEKGKECTINCLEIGAFDFITKPSGSISLDLYKIKEQLVERVVAAGLSGKRKRPVSQTVRPEPIVRAVVKPELSKPK
PGTGRQIVCIGTSTGGPRALQKVIPKLPKDLNAPVVVVQHMPEGFTASLADRLNHLSDIQVKEAKDGEAALNGCVYIAPG
GKNISVIKNSEGLQVVLDNHDTPSRHKPSADYLFRSVGKLTDYEKVAVIMTGMGSDGTAGLKDMLTAGNVKAIAESEESC
VVYGMPKAAVKAGLIHEIKHVEDIAASITSCVKKERV
>Mature_357_residues
MIRVLVVDDSAFMRKMISDFLTEEKQIEVIGTARNGEEALKKIELLKPDVITLDVEMPVMNGTDTLRKIIEIYNLPVIMV
SSQTEKGKECTINCLEIGAFDFITKPSGSISLDLYKIKEQLVERVVAAGLSGKRKRPVSQTVRPEPIVRAVVKPELSKPK
PGTGRQIVCIGTSTGGPRALQKVIPKLPKDLNAPVVVVQHMPEGFTASLADRLNHLSDIQVKEAKDGEAALNGCVYIAPG
GKNISVIKNSEGLQVVLDNHDTPSRHKPSADYLFRSVGKLTDYEKVAVIMTGMGSDGTAGLKDMLTAGNVKAIAESEESC
VVYGMPKAAVKAGLIHEIKHVEDIAASITSCVKKERV

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR. B.subtilis has an effective methylation- independent a

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=343, Percent_Identity=41.6909620991254, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI87082052, Length=106, Percent_Identity=33.0188679245283, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_BACSU (Q05522)

Other databases:

- EMBL:   X67806
- EMBL:   AL009126
- PIR:   A48511
- RefSeq:   NP_389524.2
- ProteinModelPortal:   Q05522
- SMR:   Q05522
- EnsemblBacteria:   EBBACT00000002563
- GeneID:   940124
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16420
- NMPDR:   fig|224308.1.peg.1645
- GenoList:   BSU16420
- GeneTree:   EBGT00050000000050
- HOGENOM:   HBG705324
- ProtClustDB:   PRK00742
- BioCyc:   BSUB:BSU16420-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38665; Mature: 38665

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 173-173 ACT_SITE 200-200 ACT_SITE 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLVVDDSAFMRKMISDFLTEEKQIEVIGTARNGEEALKKIELLKPDVITLDVEMPVM
CEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
NGTDTLRKIIEIYNLPVIMVSSQTEKGKECTINCLEIGAFDFITKPSGSISLDLYKIKEQ
CCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCCCCEEEHHHHHHHH
LVERVVAAGLSGKRKRPVSQTVRPEPIVRAVVKPELSKPKPGTGRQIVCIGTSTGGPRAL
HHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHH
QKVIPKLPKDLNAPVVVVQHMPEGFTASLADRLNHLSDIQVKEAKDGEAALNGCVYIAPG
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCHHHCCEEEEECC
GKNISVIKNSEGLQVVLDNHDTPSRHKPSADYLFRSVGKLTDYEKVAVIMTGMGSDGTAG
CCEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCC
LKDMLTAGNVKAIAESEESCVVYGMPKAAVKAGLIHEIKHVEDIAASITSCVKKERV
HHHHHHCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRVLVVDDSAFMRKMISDFLTEEKQIEVIGTARNGEEALKKIELLKPDVITLDVEMPVM
CEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
NGTDTLRKIIEIYNLPVIMVSSQTEKGKECTINCLEIGAFDFITKPSGSISLDLYKIKEQ
CCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCCCCEEEHHHHHHHH
LVERVVAAGLSGKRKRPVSQTVRPEPIVRAVVKPELSKPKPGTGRQIVCIGTSTGGPRAL
HHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHH
QKVIPKLPKDLNAPVVVVQHMPEGFTASLADRLNHLSDIQVKEAKDGEAALNGCVYIAPG
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCHHHCCEEEEECC
GKNISVIKNSEGLQVVLDNHDTPSRHKPSADYLFRSVGKLTDYEKVAVIMTGMGSDGTAG
CCEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCC
LKDMLTAGNVKAIAESEESCVVYGMPKAAVKAGLIHEIKHVEDIAASITSCVKKERV
HHHHHHCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8395512; 9384377