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The map label for this gene is ykuT

Identifier: 255767333

GI number: 255767333

Start: 1491221

End: 1492024

Strand: Reverse

Name: ykuT

Synonym: BSU14210

Alternate gene names: 255767333

Gene position: 1492024-1491221 (Counterclockwise)

Preceding gene: 16078489

Following gene: 255767328

Centisome position: 35.39

GC content: 41.79

Gene sequence:

>804_bases
ATGGACTTTATTAAACAATATGATTGGGCGGGACTGATTACAAATGCCGGTGTTCTGCTCATTAAACTCGCCATTATGAT
ATTGCTCTATTTTATCGTTCGATCATTAGGCATGAAAATCATTAAACATCTGTTTGCAAAATTCGAGGAGCAGAATAGCT
TGTCTATCGGACGTGCACATACGCTCCGAAGCCTGACTCTTAACATATTTGCTTATACATTGATTTTCATATTTTTCGTC
ATGGTTCTAGATTTGTTTCATTATGATCCAAGCGCTCTTTTAGCGGGGGCAGGAATCGTCGGCCTGGCTGTCGGATTTGG
CGCCCAAGGGCTTGTCAGTGATATCGTGACAGGTTTTTTTATTTTGCTTGAGAAACAATTGGATGTCGGAGATTATATTA
CCGTTTCCACCTTTGACGGAATTGTCGAGCAGGTTGGCTTAAGGACAACTCAAATCCGCAGCTTTGACGGAACACTGCAT
TACATCCCCAACAGAAACATCACAAACGTGAGCAATCATTCTCGTGGTACCATGCAGGCTCTTGTTGACATTAAAGTGCC
GGCTGAAAGAAATATTGATGAAATGATTCATATTTTACAGCAGGTTTGTGATGAAACGGCAGCGGCTCTTCCACAAATAA
AAGAAGGCCCAAATGTCATCGGGATTCAAGAACTGGGGACTTCTGAAATTGTAATCAGGGTCATTGCCAAAACAGAAAAT
ATGGAACAGTGGCGAGTCGAACGAGTGTTGAGAAAAGAAATAAAGAACGCGTTCGACCGCGCTTTCCCTAAAGAAACAGA
ATAA

Upstream 100 bases:

>100_bases
ATGTCTTTGGCTTCGACATTTTTGAATAAAACGTTTAACATGGTCATGTACAGGGTAACTAGTCTCAATGTGCAGCAATC
AATTAGAAAAGGAGCTCAAC

Downstream 100 bases:

>100_bases
ATAAAAGAACCGAAGCTTTTTGCTTCGGTTTTTTTATTACTGAATTCTGCTCTCAACTTCGCTACAAATTTCTTCAGCCG
TCATCCCGGAAGCTGTAATG

Product: small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDFIKQYDWAGLITNAGVLLIKLAIMILLYFIVRSLGMKIIKHLFAKFEEQNSLSIGRAHTLRSLTLNIFAYTLIFIFFV
MVLDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVTGFFILLEKQLDVGDYITVSTFDGIVEQVGLRTTQIRSFDGTLH
YIPNRNITNVSNHSRGTMQALVDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIAKTEN
MEQWRVERVLRKEIKNAFDRAFPKETE

Sequences:

>Translated_267_residues
MDFIKQYDWAGLITNAGVLLIKLAIMILLYFIVRSLGMKIIKHLFAKFEEQNSLSIGRAHTLRSLTLNIFAYTLIFIFFV
MVLDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVTGFFILLEKQLDVGDYITVSTFDGIVEQVGLRTTQIRSFDGTLH
YIPNRNITNVSNHSRGTMQALVDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIAKTEN
MEQWRVERVLRKEIKNAFDRAFPKETE
>Mature_267_residues
MDFIKQYDWAGLITNAGVLLIKLAIMILLYFIVRSLGMKIIKHLFAKFEEQNSLSIGRAHTLRSLTLNIFAYTLIFIFFV
MVLDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVTGFFILLEKQLDVGDYITVSTFDGIVEQVGLRTTQIRSFDGTLH
YIPNRNITNVSNHSRGTMQALVDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIAKTEN
MEQWRVERVLRKEIKNAFDRAFPKETE

Specific function: May play a role in resistance to osmotic downshock

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family

Homologues:

Organism=Escherichia coli, GI87081787, Length=264, Percent_Identity=30.3030303030303, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789291, Length=268, Percent_Identity=27.6119402985075, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787591, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YKUT_BACSU (O34897)

Other databases:

- EMBL:   AJ222587
- EMBL:   AL009126
- PIR:   A69867
- RefSeq:   NP_389304.2
- ProteinModelPortal:   O34897
- SMR:   O34897
- EnsemblBacteria:   EBBACT00000002701
- GeneID:   938809
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14210
- NMPDR:   fig|224308.1.peg.1423
- GenoList:   BSU14210
- GeneTree:   EBGT00050000001807
- HOGENOM:   HBG728528
- ProtClustDB:   CLSK873386
- BioCyc:   BSUB:BSU14210-MONOMER
- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014

Pfam domain/function: PF00924 MS_channel; SSF82689 MscC_channel_C; SSF82861 MscS_transmembr; SSF50182 Sm_like_riboprot

EC number: NA

Molecular weight: Translated: 29956; Mature: 29956

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc303a18)-; HASH(0xeb5f608)-; HASH(0xe991fdc)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIKQYDWAGLITNAGVLLIKLAIMILLYFIVRSLGMKIIKHLFAKFEEQNSLSIGRAH
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TLRSLTLNIFAYTLIFIFFVMVLDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVTGFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHH
ILLEKQLDVGDYITVSTFDGIVEQVGLRTTQIRSFDGTLHYIPNRNITNVSNHSRGTMQA
HHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHEECCCCEEEECCCCCCCCCCCCCCHHHHH
LVDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIAKTEN
HHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEHHHCCCHHHHHHHHHHCCC
MEQWRVERVLRKEIKNAFDRAFPKETE
HHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MDFIKQYDWAGLITNAGVLLIKLAIMILLYFIVRSLGMKIIKHLFAKFEEQNSLSIGRAH
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TLRSLTLNIFAYTLIFIFFVMVLDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVTGFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHH
ILLEKQLDVGDYITVSTFDGIVEQVGLRTTQIRSFDGTLHYIPNRNITNVSNHSRGTMQA
HHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHEECCCCEEEECCCCCCCCCCCCCCHHHHH
LVDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIAKTEN
HHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEHHHCCCHHHHHHHHHHCCC
MEQWRVERVLRKEIKNAFDRAFPKETE
HHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377