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The map label for this gene is sbcD

Identifier: 255767244

GI number: 255767244

Start: 1143577

End: 1144752

Strand: Direct

Name: sbcD

Synonym: BSU10640

Alternate gene names: 255767244

Gene position: 1143577-1144752 (Clockwise)

Preceding gene: 255767243

Following gene: 255767245

Centisome position: 27.13

GC content: 51.53

Gene sequence:

>1176_bases
TTGCGGATTTTACATACGGCTGACTGGCATCTTGGAAAAACGCTTGAAGGAAGAAGCAGGCTGAGTGAACAGGCCGATGT
GCTGGATGAACTGAATACGATTGTAAAGGATGAGCAGATCGATGCCATTGTAATGGCGGGGGATGCATTTGACACCGTAA
ACCCGCCAGCTTTAGCCGAGCAGCTGTTTTATGAAAGCCTGTCTGCGCTTAGCGACAGAGGAAAGCGCCCGATCGTCGTC
ATTGCCGGAAATCACGATAATCCTGACCGTTTGTCCGCCGCTTCACCGCTGACACATGAAAACGGCATTCATTTAATCGG
TTATCCGACAACAGAGCCGATTCATATTGAAGTGCCTTCAGCAGGAGAGCTTTTGGCGGTTGGAGCGCTGGCGTACCCAT
CTGAAGCGCGGTTAAATGAAGTGCTATCCGATACGTTTGATGAAAAGCTGCTCCGTGATCACTATGATGTGAAAATCAGG
CAGGCGTTTGAGCATATGACAAGCCGGTTCCGGACCGATGCAGTGAAGATTGCCGCCAGCCATATTTATGTGGCAGGCGG
AAACCAAACCGATTCAGAGCGGCCGATTGAAGTCGGCGGTGCATACACGGTGGCTGCTGAAAGCCTGCCGGCAGATGCCG
CTTACGTTGCGCTCGGCCATTTGCATCGCCCGCAAACGATCAAGCGGGCGCGGACGCTTGCGCGTTATTCAGGATCTCCG
CTCGCCTACAGCTTTTCTGAAGCGGGCTACGCTAAATCAGTGACGATTGTGGACGCAAAGCCTGGGGAAGAGGCCACTTG
GCAAGAGGTGTTATTATCAAGCGGCAAGCCACTTGTGAAGTGGAAAGCGGCAAATGGATTAAGTGAGGTGTACAGCTGGC
TGGATGAAGGCAGAGATCAGAATGCTTGGATTGACTTGGAAATCCGTGTCGCGGACCAGCTGTCACTTGAAGAGATTCAC
AGGCTGCGGAAGGCGCACCCGGGCTTTATCCATATCAGGCCGGTTTTTGAAGAACAGAACAAAGACCGGGAACGGATCGA
GGTTAAACATGTCTCAATCGAGGACCGCTTTAAGAAATTTTATGAAAAACAAACTGGCGGTGCGGTGCCGGATGAAGAAA
TGGTGAAGCTGTTTTTAGAGCTTGCATCAGGCGTTGAAGAGGAGGATGCGAAATGA

Upstream 100 bases:

>100_bases
TTGACGGAGGGCACATTCTGACATTATAGCGAGATCCATAAGCTCCGGAATTTCAGGCGGAGCGGGTCTCGTTTTCTATA
TAAAAGAGAGGTGAAAGCAT

Downstream 100 bases:

>100_bases
AGCCGATCGCCTTAAGCATTAAGGGGCTCCACAGCTTTAGAGAGGAGCAGACGATAGATTTTGAAGGCCTTTCCGGTGCC
GGTGTTTTCGGCATTTTCGG

Product: DNA repair exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRILHTADWHLGKTLEGRSRLSEQADVLDELNTIVKDEQIDAIVMAGDAFDTVNPPALAEQLFYESLSALSDRGKRPIVV
IAGNHDNPDRLSAASPLTHENGIHLIGYPTTEPIHIEVPSAGELLAVGALAYPSEARLNEVLSDTFDEKLLRDHYDVKIR
QAFEHMTSRFRTDAVKIAASHIYVAGGNQTDSERPIEVGGAYTVAAESLPADAAYVALGHLHRPQTIKRARTLARYSGSP
LAYSFSEAGYAKSVTIVDAKPGEEATWQEVLLSSGKPLVKWKAANGLSEVYSWLDEGRDQNAWIDLEIRVADQLSLEEIH
RLRKAHPGFIHIRPVFEEQNKDRERIEVKHVSIEDRFKKFYEKQTGGAVPDEEMVKLFLELASGVEEEDAK

Sequences:

>Translated_391_residues
MRILHTADWHLGKTLEGRSRLSEQADVLDELNTIVKDEQIDAIVMAGDAFDTVNPPALAEQLFYESLSALSDRGKRPIVV
IAGNHDNPDRLSAASPLTHENGIHLIGYPTTEPIHIEVPSAGELLAVGALAYPSEARLNEVLSDTFDEKLLRDHYDVKIR
QAFEHMTSRFRTDAVKIAASHIYVAGGNQTDSERPIEVGGAYTVAAESLPADAAYVALGHLHRPQTIKRARTLARYSGSP
LAYSFSEAGYAKSVTIVDAKPGEEATWQEVLLSSGKPLVKWKAANGLSEVYSWLDEGRDQNAWIDLEIRVADQLSLEEIH
RLRKAHPGFIHIRPVFEEQNKDRERIEVKHVSIEDRFKKFYEKQTGGAVPDEEMVKLFLELASGVEEEDAK
>Mature_391_residues
MRILHTADWHLGKTLEGRSRLSEQADVLDELNTIVKDEQIDAIVMAGDAFDTVNPPALAEQLFYESLSALSDRGKRPIVV
IAGNHDNPDRLSAASPLTHENGIHLIGYPTTEPIHIEVPSAGELLAVGALAYPSEARLNEVLSDTFDEKLLRDHYDVKIR
QAFEHMTSRFRTDAVKIAASHIYVAGGNQTDSERPIEVGGAYTVAAESLPADAAYVALGHLHRPQTIKRARTLARYSGSP
LAYSFSEAGYAKSVTIVDAKPGEEATWQEVLLSSGKPLVKWKAANGLSEVYSWLDEGRDQNAWIDLEIRVADQLSLEEIH
RLRKAHPGFIHIRPVFEEQNKDRERIEVKHVSIEDRFKKFYEKQTGGAVPDEEMVKLFLELASGVEEEDAK

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family

Homologues:

Organism=Escherichia coli, GI1786598, Length=327, Percent_Identity=30.2752293577982, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SBCD_BACSU (P23479)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- EMBL:   M63489
- PIR:   C39432
- RefSeq:   NP_388945.3
- ProteinModelPortal:   P23479
- SMR:   P23479
- EnsemblBacteria:   EBBACT00000001955
- GeneID:   936351
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10640
- GenoList:   BSU10640
- GeneTree:   EBGT00050000002293
- HOGENOM:   HBG677966
- PhylomeDB:   P23479
- ProtClustDB:   CLSK883263
- BioCyc:   BSUB:BSU10640-MONOMER
- InterPro:   IPR004843
- InterPro:   IPR004593
- TIGRFAMs:   TIGR00619

Pfam domain/function: PF00149 Metallophos

EC number: NA

Molecular weight: Translated: 43492; Mature: 43492

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTADWHLGKTLEGRSRLSEQADVLDELNTIVKDEQIDAIVMAGDAFDTVNPPALAE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
QLFYESLSALSDRGKRPIVVIAGNHDNPDRLSAASPLTHENGIHLIGYPTTEPIHIEVPS
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCCCEEEEECCCCCCEEEECCC
AGELLAVGALAYPSEARLNEVLSDTFDEKLLRDHYDVKIRQAFEHMTSRFRTDAVKIAAS
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEE
HIYVAGGNQTDSERPIEVGGAYTVAAESLPADAAYVALGHLHRPQTIKRARTLARYSGSP
EEEEECCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LAYSFSEAGYAKSVTIVDAKPGEEATWQEVLLSSGKPLVKWKAANGLSEVYSWLDEGRDQ
CEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCC
NAWIDLEIRVADQLSLEEIHRLRKAHPGFIHIRPVFEEQNKDRERIEVKHVSIEDRFKKF
CCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHEEEEECCHHHHHHHH
YEKQTGGAVPDEEMVKLFLELASGVEEEDAK
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRILHTADWHLGKTLEGRSRLSEQADVLDELNTIVKDEQIDAIVMAGDAFDTVNPPALAE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
QLFYESLSALSDRGKRPIVVIAGNHDNPDRLSAASPLTHENGIHLIGYPTTEPIHIEVPS
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCCCEEEEECCCCCCEEEECCC
AGELLAVGALAYPSEARLNEVLSDTFDEKLLRDHYDVKIRQAFEHMTSRFRTDAVKIAAS
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEE
HIYVAGGNQTDSERPIEVGGAYTVAAESLPADAAYVALGHLHRPQTIKRARTLARYSGSP
EEEEECCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCC
LAYSFSEAGYAKSVTIVDAKPGEEATWQEVLLSSGKPLVKWKAANGLSEVYSWLDEGRDQ
CEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCC
NAWIDLEIRVADQLSLEEIHRLRKAHPGFIHIRPVFEEQNKDRERIEVKHVSIEDRFKKF
CCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHEEEEECCHHHHHHHH
YEKQTGGAVPDEEMVKLFLELASGVEEEDAK
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111