Click here to switch to the map view.

The map label for this gene is ntdB

Identifier: 255767241

GI number: 255767241

Start: 1127466

End: 1128314

Strand: Reverse

Name: ntdB

Synonym: BSU10540

Alternate gene names: 255767241

Gene position: 1128314-1127466 (Counterclockwise)

Preceding gene: 16078119

Following gene: 255767240

Centisome position: 26.77

GC content: 35.1

Gene sequence:

>849_bases
ATGTTATTAAGCAAGAAATCGGAGTATAAAACGTTATCCACGGTTGAACATCCTCAATATATTGTTTTTTGTGATTTTGA
TGAAACGTATTTTCCGCATACAATCGACGAACAAAAGCAACAAGACATCTATGAACTCGAAGACTATCTAGAACAAAAAA
GTAAAGATGGGGAGCTAATAATCGGATGGGTTACTGGGAGCAGTATAGAATCTATTCTCGATAAAATGGGACGAGGAAAA
TTTAGATATTTTCCGCATTTTATAGCTAGTGATCTTGGAACTGAAATTACGTACTTTTCAGAGCATAACTTCGGTCAGCA
AGATAACAAGTGGAACAGTCGCATAAATGAAGGGTTTAGTAAAGAAAAGGTTGAAAAACTTGTGAAACAGCTGCATGAAA
ATCATAACATTCTTTTGAATCCTCAAACCCAATTAGGAAAGTCACGGTATAAGCATAACTTCTACTATCAGGAACAAGAT
GAAATAAACGACAAGAAAAACCTATTAGCTATTGAAAAAATCTGCGAAGAATACGGCGTTTCAGTAAATATAAATCGGTG
TAATCCCTTAGCAGGCGATCCAGAAGACAGCTATGATGTAGATTTTATCCCCATAGGGACAGGAAAGAATGAAATTGTAA
CGTTTATGTTAGAGAAATACAACCTAAATACCGAAAGAGCTATCGCATTTGGGGATAGTGGAAATGATGTTCGTATGTTA
CAGACAGTAGGGAATGGATATCTGCTAAAAAATGCAACACAGGAAGCCAAAAACTTGCACAACCTTATAACTGATAGTGA
GTACTCAAAAGGAATTACTAATACCTTAAAAAAATTAATTGGATCATGA

Upstream 100 bases:

>100_bases
TTGGTTCATACGGAAAAGGCTCATAGTCAGGTACTGCATTTGCCTTTATATCCGTCATTTACTTTAGAAGAACAAGACAG
AGTAATGGAGGGACTGTTTC

Downstream 100 bases:

>100_bases
GGAGGAAATAAAAATGAAGAAGATAGGCATCATAGGTGCAGGTGGTATTGCAAGAGCGCATGCAACTGCTTTATCTACAA
TAAAAAATGCAGAGTTAGTA

Product: neotrehalosadiamine biosynthesis hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLLSKKSEYKTLSTVEHPQYIVFCDFDETYFPHTIDEQKQQDIYELEDYLEQKSKDGELIIGWVTGSSIESILDKMGRGK
FRYFPHFIASDLGTEITYFSEHNFGQQDNKWNSRINEGFSKEKVEKLVKQLHENHNILLNPQTQLGKSRYKHNFYYQEQD
EINDKKNLLAIEKICEEYGVSVNINRCNPLAGDPEDSYDVDFIPIGTGKNEIVTFMLEKYNLNTERAIAFGDSGNDVRML
QTVGNGYLLKNATQEAKNLHNLITDSEYSKGITNTLKKLIGS

Sequences:

>Translated_282_residues
MLLSKKSEYKTLSTVEHPQYIVFCDFDETYFPHTIDEQKQQDIYELEDYLEQKSKDGELIIGWVTGSSIESILDKMGRGK
FRYFPHFIASDLGTEITYFSEHNFGQQDNKWNSRINEGFSKEKVEKLVKQLHENHNILLNPQTQLGKSRYKHNFYYQEQD
EINDKKNLLAIEKICEEYGVSVNINRCNPLAGDPEDSYDVDFIPIGTGKNEIVTFMLEKYNLNTERAIAFGDSGNDVRML
QTVGNGYLLKNATQEAKNLHNLITDSEYSKGITNTLKKLIGS
>Mature_282_residues
MLLSKKSEYKTLSTVEHPQYIVFCDFDETYFPHTIDEQKQQDIYELEDYLEQKSKDGELIIGWVTGSSIESILDKMGRGK
FRYFPHFIASDLGTEITYFSEHNFGQQDNKWNSRINEGFSKEKVEKLVKQLHENHNILLNPQTQLGKSRYKHNFYYQEQD
EINDKKNLLAIEKICEEYGVSVNINRCNPLAGDPEDSYDVDFIPIGTGKNEIVTFMLEKYNLNTERAIAFGDSGNDVRML
QTVGNGYLLKNATQEAKNLHNLITDSEYSKGITNTLKKLIGS

Specific function: Part of the ntdABC operon that biosynthesizes the NTD (3,3'-neotrehalosadiamine) antibiotic

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NTDB_BACSU (O07565)

Other databases:

- EMBL:   Y14081
- EMBL:   AL009126
- PIR:   B69834
- RefSeq:   NP_388935.2
- PDB:   3GYG
- PDBsum:   3GYG
- ProteinModelPortal:   O07565
- SMR:   O07565
- EnsemblBacteria:   EBBACT00000000944
- GeneID:   939323
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10540
- NMPDR:   fig|224308.1.peg.1054
- GenoList:   BSU10540
- GeneTree:   EBGT00050000001016
- HOGENOM:   HBG566946
- ProtClustDB:   CLSK2393375
- BioCyc:   BSUB:BSU10540-MONOMER
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150
- InterPro:   IPR006380
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01484

Pfam domain/function: PF05116 S6PP; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 32569; Mature: 32569

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01228 COF_1; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSKKSEYKTLSTVEHPQYIVFCDFDETYFPHTIDEQKQQDIYELEDYLEQKSKDGELI
CCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
IGWVTGSSIESILDKMGRGKFRYFPHFIASDLGTEITYFSEHNFGQQDNKWNSRINEGFS
EEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCC
KEKVEKLVKQLHENHNILLNPQTQLGKSRYKHNFYYQEQDEINDKKNLLAIEKICEEYGV
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCC
SVNINRCNPLAGDPEDSYDVDFIPIGTGKNEIVTFMLEKYNLNTERAIAFGDSGNDVRML
EEEEECCCCCCCCCCCCCCEEEEECCCCCCHHEEEEHHHHCCCCCCEEEECCCCCCEEEE
QTVGNGYLLKNATQEAKNLHNLITDSEYSKGITNTLKKLIGS
EECCCCEEECCHHHHHHHHHHHHCCCHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MLLSKKSEYKTLSTVEHPQYIVFCDFDETYFPHTIDEQKQQDIYELEDYLEQKSKDGELI
CCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
IGWVTGSSIESILDKMGRGKFRYFPHFIASDLGTEITYFSEHNFGQQDNKWNSRINEGFS
EEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCC
KEKVEKLVKQLHENHNILLNPQTQLGKSRYKHNFYYQEQDEINDKKNLLAIEKICEEYGV
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCC
SVNINRCNPLAGDPEDSYDVDFIPIGTGKNEIVTFMLEKYNLNTERAIAFGDSGNDVRML
EEEEECCCCCCCCCCCCCCEEEEECCCCCCHHEEEEHHHHCCCCCCEEEECCCCCCEEEE
QTVGNGYLLKNATQEAKNLHNLITDSEYSKGITNTLKKLIGS
EECCCCEEECCHHHHHHHHHHHHCCCHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377