Click here to switch to the map view.

The map label for this gene is aprE

Identifier: 255767238

GI number: 255767238

Start: 1104423

End: 1105568

Strand: Reverse

Name: aprE

Synonym: BSU10300

Alternate gene names: 255767238

Gene position: 1105568-1104423 (Counterclockwise)

Preceding gene: 16078098

Following gene: 16078092

Centisome position: 26.23

GC content: 46.42

Gene sequence:

>1146_bases
GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAATCTTTACGATGGCGTTCAGCAACATGTCTGC
GCAGGCTGCCGGAAAAAGCAGTACAGAAAAGAAATACATTGTCGGATTTAAACAGACAATGAGTGCCATGAGTTCCGCCA
AGAAAAAGGATGTTATTTCTGAAAAAGGCGGAAAGGTTCAAAAGCAATTTAAGTATGTTAACGCGGCCGCAGCAACATTG
GATGAAAAAGCTGTAAAAGAATTGAAAAAAGATCCGAGCGTTGCATATGTGGAAGAAGATCATATTGCACATGAATATGC
GCAATCTGTTCCTTATGGCATTTCTCAAATTAAAGCGCCGGCTCTTCACTCTCAAGGCTACACAGGCTCTAACGTAAAAG
TAGCTGTTATCGACAGCGGAATTGACTCTTCTCATCCTGACTTAAACGTCAGAGGCGGAGCAAGCTTCGTACCTTCTGAA
ACAAACCCATACCAGGACGGCAGTTCTCACGGTACGCATGTAGCCGGTACGATTGCCGCTCTTAATAACTCAATCGGTGT
TCTGGGCGTAGCGCCAAGCGCATCATTATATGCAGTAAAAGTGCTTGATTCAACAGGAAGCGGCCAATATAGCTGGATTA
TTAACGGCATTGAGTGGGCCATTTCCAACAATATGGATGTTATCAACATGAGCCTTGGCGGACCTACTGGTTCTACAGCG
CTGAAAACAGTCGTTGACAAAGCCGTTTCCAGCGGTATCGTCGTTGCTGCCGCAGCCGGAAACGAAGGTTCATCCGGAAG
CACAAGCACAGTCGGCTACCCTGCAAAATATCCTTCTACTATTGCAGTAGGTGCGGTAAACAGCAGCAACCAAAGAGCTT
CATTCTCCAGCGCAGGTTCTGAGCTTGATGTGATGGCTCCTGGCGTGTCCATCCAAAGCACACTTCCTGGAGGCACTTAC
GGCGCTTATAACGGAACGTCCATGGCGACTCCTCACGTTGCCGGAGCAGCAGCGTTAATTCTTTCTAAGCACCCGACTTG
GACAAACGCGCAAGTCCGTGATCGTTTAGAAAGCACTGCAACATATCTTGGAAACTCTTTCTACTATGGAAAAGGGTTAA
TCAACGTACAAGCAGCTGCACAATAA

Upstream 100 bases:

>100_bases
ATGGGTCTACTAAAATATTATTCCATCTATTACAATAAATTCACAGAATAGTCTTTTAAGTAAGTCTACTCTGAATTTTT
TTAAAAGGAGAGGGTAAAGA

Downstream 100 bases:

>100_bases
TAGTAAAAAGAAGCAGGTTCCTCCATACCTGCTTCTTTTTATTTGTCAGCATCCTGATGTTCCGGCGCATTCTCTTCTTT
CTCCGCATGTTGAATCCGTT

Product: serine alkaline protease (subtilisin E)

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATL
DEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSE
TNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTA
LKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTY
GAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ

Sequences:

>Translated_381_residues
MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATL
DEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSE
TNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTA
LKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTY
GAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ
>Mature_381_residues
MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATL
DEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSE
TNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTA
LKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTY
GAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=303, Percent_Identity=31.023102310231, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI31317307, Length=293, Percent_Identity=31.0580204778157, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI186972143, Length=279, Percent_Identity=31.8996415770609, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71988308, Length=267, Percent_Identity=30.3370786516854, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71988301, Length=267, Percent_Identity=30.7116104868914, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71988312, Length=267, Percent_Identity=30.3370786516854, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988315, Length=267, Percent_Identity=30.3370786516854, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988321, Length=267, Percent_Identity=30.3370786516854, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988297, Length=267, Percent_Identity=30.3370786516854, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71987206, Length=288, Percent_Identity=27.4305555555556, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983555, Length=267, Percent_Identity=25.4681647940075, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320775, Length=316, Percent_Identity=35.4430379746835, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324576, Length=291, Percent_Identity=32.6460481099656, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319893, Length=237, Percent_Identity=32.4894514767932, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45550681, Length=243, Percent_Identity=31.2757201646091, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17137360, Length=256, Percent_Identity=31.640625, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24653270, Length=256, Percent_Identity=31.640625, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136192, Length=264, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 39480; Mature: 39480

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVIS
CCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
EKGGKVQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHCCC
ALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAA
CCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
LNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTA
HCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCHHH
LKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGS
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCCC
ELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTA
EEEEECCCCEEECCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHH
TYLGNSFYYGKGLINVQAAAQ
HHHCCCEEECCCEEEEEECCC
>Mature Secondary Structure
MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKSSTEKKYIVGFKQTMSAMSSAKKKDVIS
CCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
EKGGKVQKQFKYVNAAAATLDEKAVKELKKDPSVAYVEEDHIAHEYAQSVPYGISQIKAP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHCCC
ALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAA
CCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
LNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTA
HCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCHHH
LKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGS
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCCC
ELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTA
EEEEECCCCEEECCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHH
TYLGNSFYYGKGLINVQAAAQ
HHHCCCEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]