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The map label for this gene is yhaA

Identifier: 255767235

GI number: 255767235

Start: 1080150

End: 1081340

Strand: Reverse

Name: yhaA

Synonym: BSU10070

Alternate gene names: 255767235

Gene position: 1081340-1080150 (Counterclockwise)

Preceding gene: 16078072

Following gene: 16078067

Centisome position: 25.65

GC content: 50.13

Gene sequence:

>1191_bases
TTGTCCATATCCACACTGCAGAAAGAGATAAACAAACAGCTCGACGGCTGTTTTGAAGAAATGGTTGAGATCAGGCGCCA
TTTTCATATGTATCCTGAGCTCTCATTTCAAGAAGAAAAAACCGCCGCATTTATTGCTTCCTATTATGAATCGTTAGGAG
TCCCAATCCGCACAAACGTTGGCGGTAGAGGGGTTTTAGCAAATATAGAAGGAAGCGAACCCGGCCCTACAGTCGCTTTG
AGGGCCGACTTTGACGCTCTCCCCATTCAAGATGAAAAAGATGTCCCTTACGCCTCCAAAGTGCCTGGTGTCATGCATGC
ATGCGGCCACGACGGCCACACCGCAGCTCTTCTCGCAGTGGCCAAGGTCCTTCACCAAAACAGACATGAACTGAAGGGAA
CATTTGTGATGATCCACCAGCATGCAGAAGAATATTATCCTGGAGGCGCAAAGCCAATGATTGATGACGGATGTCTCGAA
AACACGGATGTGATATTCGGCACTCATCTTTGGGCAACTGAGCCGCTCGGAACTATTCTCTGCCGCCCCGGCGCCGTAAT
GGCGGCGGCAGACCGATTTACGATTAAAGTCTTCGGAAAGGGCGGCCACGGCGCTCATCCGCATGATACTAAAGACGCCG
TCCTAATCGGTTCGCAAATCGTTTCCTCTTTGCAGCACATTGTCAGCCGCAAAGTCAATCCGATTCAATCCGCCGTCATT
TCGACAGGCTCCTTCATCGCCGACAATCCGTTTAATGTCATCGCAGACCAAGCAGTACTCATCGGCACAGCGCGTTCTTT
TGATGAAAATGTCCGGGACATTCTGGAGAAAGAAATTGAAGCGGTTGTAAAAGGAATATGCAGCATGCACGGCGCGTCCT
ATGAGTACACCTATGAACAGGGTTATCCAGCGGTTGTGAACCATCCTGCAGAAACGAACCACTTGGTGAGCACCGCAAAG
AATACCGAGGGCGTTCAGCAGGTCATTGACGGTGAACCACAAATGGGCGGCGAGGATTTTGCTTATTACTTACAAAACGT
GAAGGGCACTTTTTTCTTTACAGGCGCCGCTCCCGAACAGCCAGAGCGAGTCTATTCCCACCACCATCCGAAATTTGATA
TCAACGAAAAAGCCATGCTGACAGCGGCCAAGGTCCTTGCCGGCGCTGCGATCACCTATCATCAGCTATAA

Upstream 100 bases:

>100_bases
AACCCTCGCAGCTGTGATGATGCTTTAAAACAGCATGAGTTGAAATATTCAGACTTTTTCTATACAATCATGGAAAAGCA
TAGAAAAGGGGGAAGCGGCT

Downstream 100 bases:

>100_bases
AAAAACAGCCGGAGTGTTTATTCTCCGGCTGTTTCCTTTAATATCCTCAGATGAAAAACATGTCTTGCCGTTTCTCCAAG
CTGAGCAAGCAGCTTATAAT

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSISTLQKEINKQLDGCFEEMVEIRRHFHMYPELSFQEEKTAAFIASYYESLGVPIRTNVGGRGVLANIEGSEPGPTVAL
RADFDALPIQDEKDVPYASKVPGVMHACGHDGHTAALLAVAKVLHQNRHELKGTFVMIHQHAEEYYPGGAKPMIDDGCLE
NTDVIFGTHLWATEPLGTILCRPGAVMAAADRFTIKVFGKGGHGAHPHDTKDAVLIGSQIVSSLQHIVSRKVNPIQSAVI
STGSFIADNPFNVIADQAVLIGTARSFDENVRDILEKEIEAVVKGICSMHGASYEYTYEQGYPAVVNHPAETNHLVSTAK
NTEGVQQVIDGEPQMGGEDFAYYLQNVKGTFFFTGAAPEQPERVYSHHHPKFDINEKAMLTAAKVLAGAAITYHQL

Sequences:

>Translated_396_residues
MSISTLQKEINKQLDGCFEEMVEIRRHFHMYPELSFQEEKTAAFIASYYESLGVPIRTNVGGRGVLANIEGSEPGPTVAL
RADFDALPIQDEKDVPYASKVPGVMHACGHDGHTAALLAVAKVLHQNRHELKGTFVMIHQHAEEYYPGGAKPMIDDGCLE
NTDVIFGTHLWATEPLGTILCRPGAVMAAADRFTIKVFGKGGHGAHPHDTKDAVLIGSQIVSSLQHIVSRKVNPIQSAVI
STGSFIADNPFNVIADQAVLIGTARSFDENVRDILEKEIEAVVKGICSMHGASYEYTYEQGYPAVVNHPAETNHLVSTAK
NTEGVQQVIDGEPQMGGEDFAYYLQNVKGTFFFTGAAPEQPERVYSHHHPKFDINEKAMLTAAKVLAGAAITYHQL
>Mature_395_residues
SISTLQKEINKQLDGCFEEMVEIRRHFHMYPELSFQEEKTAAFIASYYESLGVPIRTNVGGRGVLANIEGSEPGPTVALR
ADFDALPIQDEKDVPYASKVPGVMHACGHDGHTAALLAVAKVLHQNRHELKGTFVMIHQHAEEYYPGGAKPMIDDGCLEN
TDVIFGTHLWATEPLGTILCRPGAVMAAADRFTIKVFGKGGHGAHPHDTKDAVLIGSQIVSSLQHIVSRKVNPIQSAVIS
TGSFIADNPFNVIADQAVLIGTARSFDENVRDILEKEIEAVVKGICSMHGASYEYTYEQGYPAVVNHPAETNHLVSTAKN
TEGVQQVIDGEPQMGGEDFAYYLQNVKGTFFFTGAAPEQPERVYSHHHPKFDINEKAMLTAAKVLAGAAITYHQL

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family

Homologues:

Organism=Escherichia coli, GI87081880, Length=441, Percent_Identity=26.984126984127, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHAA_BACSU (O07598)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_388888.2
- HSSP:   P54970
- ProteinModelPortal:   O07598
- SMR:   O07598
- MEROPS:   M20.008
- EnsemblBacteria:   EBBACT00000003538
- GeneID:   936299
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10070
- GenoList:   BSU10070
- GeneTree:   EBGT00070000031917
- HOGENOM:   HBG708500
- ProtClustDB:   CLSK886992
- BioCyc:   BSUB:BSU10070-MONOMER
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: NA

Molecular weight: Translated: 43220; Mature: 43089

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 85-85 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISTLQKEINKQLDGCFEEMVEIRRHFHMYPELSFQEEKTAAFIASYYESLGVPIRTNV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECC
GGRGVLANIEGSEPGPTVALRADFDALPIQDEKDVPYASKVPGVMHACGHDGHTAALLAV
CCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCHHHHHCCCCCHHHHHHHH
AKVLHQNRHELKGTFVMIHQHAEEYYPGGAKPMIDDGCLENTDVIFGTHLWATEPLGTIL
HHHHHCCHHHHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHEE
CRPGAVMAAADRFTIKVFGKGGHGAHPHDTKDAVLIGSQIVSSLQHIVSRKVNPIQSAVI
ECCCCEEEECCEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHH
STGSFIADNPFNVIADQAVLIGTARSFDENVRDILEKEIEAVVKGICSMHGASYEYTYEQ
HCCCEECCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GYPAVVNHPAETNHLVSTAKNTEGVQQVIDGEPQMGGEDFAYYLQNVKGTFFFTGAAPEQ
CCCCEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCC
PERVYSHHHPKFDINEKAMLTAAKVLAGAAITYHQL
CHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SISTLQKEINKQLDGCFEEMVEIRRHFHMYPELSFQEEKTAAFIASYYESLGVPIRTNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECC
GGRGVLANIEGSEPGPTVALRADFDALPIQDEKDVPYASKVPGVMHACGHDGHTAALLAV
CCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCHHHHHCCCCCHHHHHHHH
AKVLHQNRHELKGTFVMIHQHAEEYYPGGAKPMIDDGCLENTDVIFGTHLWATEPLGTIL
HHHHHCCHHHHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHEE
CRPGAVMAAADRFTIKVFGKGGHGAHPHDTKDAVLIGSQIVSSLQHIVSRKVNPIQSAVI
ECCCCEEEECCEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHH
STGSFIADNPFNVIADQAVLIGTARSFDENVRDILEKEIEAVVKGICSMHGASYEYTYEQ
HCCCEECCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GYPAVVNHPAETNHLVSTAKNTEGVQQVIDGEPQMGGEDFAYYLQNVKGTFFFTGAAPEQ
CCCCEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCC
PERVYSHHHPKFDINEKAMLTAAKVLAGAAITYHQL
CHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377