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The map label for this gene is yhxA

Identifier: 255767216

GI number: 255767216

Start: 1000364

End: 1001716

Strand: Direct

Name: yhxA

Synonym: BSU09260

Alternate gene names: 255767216

Gene position: 1000364-1001716 (Clockwise)

Preceding gene: 255767215

Following gene: 16077992

Centisome position: 23.73

GC content: 50.85

Gene sequence:

>1353_bases
ATGGAGATGATGGGGATGGAAAACATTCAGCAAAATCAGGGATTAAAGCAAAAAGATGAGCAATTTGTGTGGCATGCCAT
GAAGGGAGCGCATCAAGCGGACAGCCTGATAGCCCAGAAGGCCGAAGGGGCCTGGGTAACCGACACAGACGGACGCCGCT
ATTTGGATGCGATGTCCGGTTTGTGGTGCGTCAACATTGGTTACGGCAGAAAGGAGCTTGCGGAGGCTGCCTATGAGCAA
CTAAAGGAGCTGCCTTACTACCCGTTAACGCAAAGTCACGCACCCGCAATTCAACTGGCGGAAAAGCTGAATGAATGGCT
TGGCGGCGATTATGTTATTTTTTTTTCCAACAGCGGATCGGAAGCAAACGAAACTGCTTTTAAAATTGCCCGCCAGTACC
ATCTGCAAAACGGCGACCACAGCCGTTATAAATTCATCTCAAGATATCGGGCATACCACGGCAATACATTGGGAGCGCTC
TCAGCTACCGGACAGGCGCAGCGGAAATATAAATACGAGCCTTTGAGCCAAGGGTTCCTGCATGCAGCTCCGCCAGATAT
ATACCGGAATCCTGATGATGCAGACACGCTTGAAAGCGCAAATGAAATCGACCGCATCATGACATGGGAATTAAGCGAAA
CGATTGCCGGGGTCATTATGGAGCCCATCATTACAGGCGGAGGCATCCTAATGCCGCCGGACGGATATATGAAGAAGGTG
GAGGACATTTGCCGGCGCCACGGAGCCCTTTTGATTTGCGATGAAGTGATCTGCGGGTTTGGACGGACAGGTGAGCCGTT
CGGGTTTATGCACTACGGTGTGAAGCCTGATATCATTACGATGGCAAAGGGAATCACAAGCGCGTATCTGCCATTGTCAG
CGACTGCTGTGAAACGGGACATTTTCGAAGCGTATCAGGGGGAAGCTCCTTATGACCGTTTCCGCCACGTGAACACGTTC
GGCGGAAGCCCGGCTGCCTGTGCTTTGGCGTTGAAAAACCTGCAAATTATGGAGGACGAACAGCTGATTCAGCGATCCCG
TGATCTTGGAGCAAAGCTTTTAGGTGAGCTTCAAGCTCTGAGAGAACACCCGGCAGTCGGGGATGTTAGAGGAAAAGGGC
TGCTGATCGGAATCGAACTCGTCAAAGACAAATTGACTAAAGAGCCGGCTGATGCCGCCAAAGTAAACCAAGTGGTTGCG
GCGTGCAAAGAAAAAGGGCTGATCATCGGCAAAAACGGCGATACAGTCGCCGGCTACAACAATGTCATCCAGCTTGCGCC
GCCATTTTGCCTGACAGAAGAGGACCTTTCCTTTATCGTGAAAACGGTGAAAGAAAGCTTTCAAACGATATAA

Upstream 100 bases:

>100_bases
GGTCTGGCTTTATATTGAAGGCAGGGAAATCATCACCAAGACATCACATAAAGAGAAGGTCTGTTTCAATTGAATTGGCA
TTATAGGGAACGCTAATGAA

Downstream 100 bases:

>100_bases
TATAAAAGTGAATTATTAAAGGAGCACATGATGAGTTTTCACAACCAGCCGATCTTACCGGCTATTCGCAATATGAAGCA
ATTTGATGAGTTTTTGAACA

Product: aminotransferase

Products: L-Glutamate; L-Glutamate 5-semialdehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MEMMGMENIQQNQGLKQKDEQFVWHAMKGAHQADSLIAQKAEGAWVTDTDGRRYLDAMSGLWCVNIGYGRKELAEAAYEQ
LKELPYYPLTQSHAPAIQLAEKLNEWLGGDYVIFFSNSGSEANETAFKIARQYHLQNGDHSRYKFISRYRAYHGNTLGAL
SATGQAQRKYKYEPLSQGFLHAAPPDIYRNPDDADTLESANEIDRIMTWELSETIAGVIMEPIITGGGILMPPDGYMKKV
EDICRRHGALLICDEVICGFGRTGEPFGFMHYGVKPDIITMAKGITSAYLPLSATAVKRDIFEAYQGEAPYDRFRHVNTF
GGSPAACALALKNLQIMEDEQLIQRSRDLGAKLLGELQALREHPAVGDVRGKGLLIGIELVKDKLTKEPADAAKVNQVVA
ACKEKGLIIGKNGDTVAGYNNVIQLAPPFCLTEEDLSFIVKTVKESFQTI

Sequences:

>Translated_450_residues
MEMMGMENIQQNQGLKQKDEQFVWHAMKGAHQADSLIAQKAEGAWVTDTDGRRYLDAMSGLWCVNIGYGRKELAEAAYEQ
LKELPYYPLTQSHAPAIQLAEKLNEWLGGDYVIFFSNSGSEANETAFKIARQYHLQNGDHSRYKFISRYRAYHGNTLGAL
SATGQAQRKYKYEPLSQGFLHAAPPDIYRNPDDADTLESANEIDRIMTWELSETIAGVIMEPIITGGGILMPPDGYMKKV
EDICRRHGALLICDEVICGFGRTGEPFGFMHYGVKPDIITMAKGITSAYLPLSATAVKRDIFEAYQGEAPYDRFRHVNTF
GGSPAACALALKNLQIMEDEQLIQRSRDLGAKLLGELQALREHPAVGDVRGKGLLIGIELVKDKLTKEPADAAKVNQVVA
ACKEKGLIIGKNGDTVAGYNNVIQLAPPFCLTEEDLSFIVKTVKESFQTI
>Mature_450_residues
MEMMGMENIQQNQGLKQKDEQFVWHAMKGAHQADSLIAQKAEGAWVTDTDGRRYLDAMSGLWCVNIGYGRKELAEAAYEQ
LKELPYYPLTQSHAPAIQLAEKLNEWLGGDYVIFFSNSGSEANETAFKIARQYHLQNGDHSRYKFISRYRAYHGNTLGAL
SATGQAQRKYKYEPLSQGFLHAAPPDIYRNPDDADTLESANEIDRIMTWELSETIAGVIMEPIITGGGILMPPDGYMKKV
EDICRRHGALLICDEVICGFGRTGEPFGFMHYGVKPDIITMAKGITSAYLPLSATAVKRDIFEAYQGEAPYDRFRHVNTF
GGSPAACALALKNLQIMEDEQLIQRSRDLGAKLLGELQALREHPAVGDVRGKGLLIGIELVKDKLTKEPADAAKVNQVVA
ACKEKGLIIGKNGDTVAGYNNVIQLAPPFCLTEEDLSFIVKTVKESFQTI

Specific function: Essential for glycerol catabolism

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI13994255, Length=444, Percent_Identity=32.8828828828829, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI37574042, Length=426, Percent_Identity=32.1596244131455, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI226442705, Length=426, Percent_Identity=32.3943661971831, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI24119277, Length=424, Percent_Identity=31.3679245283019, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI226442709, Length=363, Percent_Identity=33.8842975206612, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI4557809, Length=440, Percent_Identity=27.9545454545455, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI284507298, Length=334, Percent_Identity=29.940119760479, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI188536080, Length=421, Percent_Identity=25.1781472684086, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI38679950, Length=421, Percent_Identity=25.1781472684086, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI38679946, Length=421, Percent_Identity=25.1781472684086, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI145693181, Length=409, Percent_Identity=31.0513447432763, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789016, Length=454, Percent_Identity=32.15859030837, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789759, Length=402, Percent_Identity=29.3532338308458, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788044, Length=404, Percent_Identity=30.4455445544554, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1786991, Length=442, Percent_Identity=31.2217194570136, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=29.5238095238095, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1786349, Length=334, Percent_Identity=25.748502994012, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32564660, Length=442, Percent_Identity=30.9954751131222, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI71992977, Length=431, Percent_Identity=30.1624129930394, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25144271, Length=441, Percent_Identity=27.6643990929705, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25144274, Length=332, Percent_Identity=28.3132530120482, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=429, Percent_Identity=25.6410256410256, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=439, Percent_Identity=30.751708428246, Blast_Score=186, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6324386, Length=463, Percent_Identity=27.8617710583153, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324432, Length=415, Percent_Identity=26.9879518072289, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321456, Length=452, Percent_Identity=24.7787610619469, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161085790, Length=444, Percent_Identity=31.981981981982, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28574759, Length=439, Percent_Identity=31.4350797266515, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21356575, Length=473, Percent_Identity=29.1754756871036, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21357415, Length=427, Percent_Identity=28.1030444964871, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281366494, Length=452, Percent_Identity=24.3362831858407, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667139, Length=452, Percent_Identity=24.3362831858407, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667143, Length=452, Percent_Identity=24.3362831858407, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHXA_BACSU (P33189)

Other databases:

- EMBL:   Y14079
- EMBL:   AL009126
- EMBL:   X96983
- EMBL:   M99611
- PIR:   B69835
- RefSeq:   NP_388807.2
- ProteinModelPortal:   P33189
- SMR:   P33189
- EnsemblBacteria:   EBBACT00000000100
- GeneID:   939742
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09260
- NMPDR:   fig|224308.1.peg.926
- GenoList:   BSU09260
- GeneTree:   EBGT00070000031850
- HOGENOM:   HBG725944
- ProtClustDB:   PRK07678
- BioCyc:   BSUB:BSU09260-MONOMER
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 2.6.1.13 [C]

Molecular weight: Translated: 49877; Mature: 49877

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMMGMENIQQNQGLKQKDEQFVWHAMKGAHQADSLIAQKAEGAWVTDTDGRRYLDAMSG
CCCCCCCHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCHHHHHHHCC
LWCVNIGYGRKELAEAAYEQLKELPYYPLTQSHAPAIQLAEKLNEWLGGDYVIFFSNSGS
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCC
EANETAFKIARQYHLQNGDHSRYKFISRYRAYHGNTLGALSATGQAQRKYKYEPLSQGFL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHCCCC
HAAPPDIYRNPDDADTLESANEIDRIMTWELSETIAGVIMEPIITGGGILMPPDGYMKKV
CCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHH
EDICRRHGALLICDEVICGFGRTGEPFGFMHYGVKPDIITMAKGITSAYLPLSATAVKRD
HHHHHHCCCEEEEHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHH
IFEAYQGEAPYDRFRHVNTFGGSPAACALALKNLQIMEDEQLIQRSRDLGAKLLGELQAL
HHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHH
REHPAVGDVRGKGLLIGIELVKDKLTKEPADAAKVNQVVAACKEKGLIIGKNGDTVAGYN
HHCCCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCEECCCC
NVIQLAPPFCLTEEDLSFIVKTVKESFQTI
CEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEMMGMENIQQNQGLKQKDEQFVWHAMKGAHQADSLIAQKAEGAWVTDTDGRRYLDAMSG
CCCCCCCHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCHHHHHHHCC
LWCVNIGYGRKELAEAAYEQLKELPYYPLTQSHAPAIQLAEKLNEWLGGDYVIFFSNSGS
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCC
EANETAFKIARQYHLQNGDHSRYKFISRYRAYHGNTLGALSATGQAQRKYKYEPLSQGFL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHCCCC
HAAPPDIYRNPDDADTLESANEIDRIMTWELSETIAGVIMEPIITGGGILMPPDGYMKKV
CCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHH
EDICRRHGALLICDEVICGFGRTGEPFGFMHYGVKPDIITMAKGITSAYLPLSATAVKRD
HHHHHHCCCEEEEHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHH
IFEAYQGEAPYDRFRHVNTFGGSPAACALALKNLQIMEDEQLIQRSRDLGAKLLGELQAL
HHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHH
REHPAVGDVRGKGLLIGIELVKDKLTKEPADAAKVNQVVAACKEKGLIIGKNGDTVAGYN
HHCCCCCCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCEECCCC
NVIQLAPPFCLTEEDLSFIVKTVKESFQTI
CEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; Ornithine [C]

Specific reaction: 2-Oxoglutarate + Ornithine --> L-Glutamate + L-Glutamate 5-semialdehyde [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8969498; 8436953