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The map label for this gene is thiC

Identifier: 255767202

GI number: 255767202

Start: 955895

End: 957667

Strand: Direct

Name: thiC

Synonym: BSU08790

Alternate gene names: 255767202

Gene position: 955895-957667 (Clockwise)

Preceding gene: 255767201

Following gene: 16077946

Centisome position: 22.68

GC content: 46.19

Gene sequence:

>1773_bases
ATGCAAAACAATTCAGTGCAGCAAGCCAACATTTCCATTATGTCAAGTTTTTCAGGCAGCAAGAAGGTATACGTGGAGGG
TTCTTCTTCGGACATCCAAGTGCCGATGAGGGAGATTGCATTGAGTCCGACGACGGGATCGTTCGGCGAAGAGGAAAACG
CGCCTGTGCGTGTCTATGATACAAGCGGACCGTATACGGACCCTGAAGTAACCATTAATATTCAAGAAGGATTAAAGCCG
CTCCGCCAAAAATGGATCACAGAGCGCGGTGACGTGGAAGAGTATGAAGGTCGGGCTATAAAACCAGAAGATAACGGTTA
TAAAAAAGCAAAACCAAACGTAAGCTATCCAGGATTAAAGCGGAAACCGCTGAGGGCGAAGGCAGGTCAAAATGTCACAC
AAATGCACTATGCAAAAAAGGGGATCATTACACCTGAGATGGAATTTATCGCCATTCGTGAGCATGTGTCTCCTGAATTC
GTCAGAGACGAGGTAGCCAGCGGCCGGGCGATTATTCCTTCAAACATTAATCACCCTGAAAGTGAGCCAATGATTATCGG
CCGGAATTTTCATGTGAAGATTAATGCAAATATCGGGAATTCAGCGGTTACGTCATCCATTGAAGAAGAAGTGGAAAAAA
TGACGTGGGCCATCCGCTGGGGAGCGGATACGATGATGGATCTCTCTACCGGAAAAGATATTCATACGACGCGTGAATGG
ATTATCCGCAATTGCCCCGTGCCTGTGGGAACTGTTCCGATTTATCAGGCGCTTGAAAAAGTAAACGGCGTAGCTGAGGA
TTTAACGTGGGAGATTTATCGTGATACGCTAATCGAGCAGGCAGAGCAGGGAGTGGATTATTTTACGATTCACGCGGGTG
TTTTGCTTAGGTATGTACCGCTGACTGCCAAACGGACAACTGGTATCGTGTCACGCGGGGGAGCGATTATGGCGCAGTGG
TGTCTGGCTCATCATCAAGAAAGCTTTTTATACACACATTTTGAAGAAATATGTGAGATTATGAAAATGTATGATATTGC
TTTCTCTCTTGGGGATGGTTTGCGTCCCGGATCAATTGCTGATGCCAATGACGAAGCGCAGTTTGCTGAATTAGAGACGC
TTGGAGAGCTGACGCAAATTGCCTGGAAGCATGATGTTCAGGTGATGATTGAGGGTCCTGGACATGTTCCGATGCATAAA
ATCAAAGAGAACGTCGATAAACAAATGGACATTTGTAAAGAAGCGCCTTTTTACACATTGGGGCCGTTGACAACGGATAT
TGCTCCGGGATATGACCATATTACTTCAGCGATCGGAGCGGCGATGATCGGTTGGTACGGGACAGCAATGCTTTGTTATG
TAACGCCGAAGGAGCATTTGGGGCTGCCAAACCGTGATGATGTTCGTGAAGGAGTCATTACATATAAGATCGCTGCGCAC
GCTGCGGACCTTGCAAAAGGGCATCCCGGAGCGCAAATCCGGGATGATGCGTTATCGAAAGCCCGTTTTGAATTCCGCTG
GCGGGATCAGTTTAATTTATCTCTTGATCCGGAGAGAGCCCTTGAATACCATGATGAAACCCTGCCTGCTGAAGGAGCAA
AAACCGCTCACTTTTGTTCCATGTGCGGTCCGAAATTTTGCAGCATGAGAATTTCACAGGATATTCGGGATTATGCGAAG
AAAAACGATCTCAGTGAAGCAGAAGCCATAAATAAAGGATTAAAAGAAAAAGCAAAAGAGTTTGTCGATACGGGAAGCAA
TTTATATCAATAA

Upstream 100 bases:

>100_bases
TTTCTGACTGTAACCGGGTTCATTTATATGAATCCGGTTTTTTGTGCTGGTTATGGGCTTGATTTTGTCCTTTCAAACGT
AGAAAGGGGAAGGAAAAGTA

Downstream 100 bases:

>100_bases
AAAAAATGAAGATGGAGAATGCTCCATCTTCATTTTTTTAGAAAAACAGAGAGCGTCGAAGCGGAGGAATACTCTGTTCA
AAGCGGAAAACATTTTCTGG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MQNNSVQQANISIMSSFSGSKKVYVEGSSSDIQVPMREIALSPTTGSFGEEENAPVRVYDTSGPYTDPEVTINIQEGLKP
LRQKWITERGDVEEYEGRAIKPEDNGYKKAKPNVSYPGLKRKPLRAKAGQNVTQMHYAKKGIITPEMEFIAIREHVSPEF
VRDEVASGRAIIPSNINHPESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREW
IIRNCPVPVGTVPIYQALEKVNGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRTTGIVSRGGAIMAQW
CLAHHQESFLYTHFEEICEIMKMYDIAFSLGDGLRPGSIADANDEAQFAELETLGELTQIAWKHDVQVMIEGPGHVPMHK
IKENVDKQMDICKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAH
AADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISQDIRDYAK
KNDLSEAEAINKGLKEKAKEFVDTGSNLYQ

Sequences:

>Translated_590_residues
MQNNSVQQANISIMSSFSGSKKVYVEGSSSDIQVPMREIALSPTTGSFGEEENAPVRVYDTSGPYTDPEVTINIQEGLKP
LRQKWITERGDVEEYEGRAIKPEDNGYKKAKPNVSYPGLKRKPLRAKAGQNVTQMHYAKKGIITPEMEFIAIREHVSPEF
VRDEVASGRAIIPSNINHPESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREW
IIRNCPVPVGTVPIYQALEKVNGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRTTGIVSRGGAIMAQW
CLAHHQESFLYTHFEEICEIMKMYDIAFSLGDGLRPGSIADANDEAQFAELETLGELTQIAWKHDVQVMIEGPGHVPMHK
IKENVDKQMDICKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAH
AADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISQDIRDYAK
KNDLSEAEAINKGLKEKAKEFVDTGSNLYQ
>Mature_590_residues
MQNNSVQQANISIMSSFSGSKKVYVEGSSSDIQVPMREIALSPTTGSFGEEENAPVRVYDTSGPYTDPEVTINIQEGLKP
LRQKWITERGDVEEYEGRAIKPEDNGYKKAKPNVSYPGLKRKPLRAKAGQNVTQMHYAKKGIITPEMEFIAIREHVSPEF
VRDEVASGRAIIPSNINHPESEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREW
IIRNCPVPVGTVPIYQALEKVNGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRTTGIVSRGGAIMAQW
CLAHHQESFLYTHFEEICEIMKMYDIAFSLGDGLRPGSIADANDEAQFAELETLGELTQIAWKHDVQVMIEGPGHVPMHK
IKENVDKQMDICKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAH
AADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCSMCGPKFCSMRISQDIRDYAK
KNDLSEAEAINKGLKEKAKEFVDTGSNLYQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=613, Percent_Identity=66.557911908646, Blast_Score=847, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 65932; Mature: 65932

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNNSVQQANISIMSSFSGSKKVYVEGSSSDIQVPMREIALSPTTGSFGEEENAPVRVYD
CCCCCCCHHHHHHEECCCCCCEEEEECCCCCCCCCHHHHEECCCCCCCCCCCCCCEEEEE
TSGPYTDPEVTINIQEGLKPLRQKWITERGDVEEYEGRAIKPEDNGYKKAKPNVSYPGLK
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHCCCEECCCCCCCCCCCCCCCCCCCC
RKPLRAKAGQNVTQMHYAKKGIITPEMEFIAIREHVSPEFVRDEVASGRAIIPSNINHPE
CCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCC
SEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREW
CCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCHHHHHHH
IIRNCPVPVGTVPIYQALEKVNGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVP
HHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHC
LTAKRTTGIVSRGGAIMAQWCLAHHQESFLYTHFEEICEIMKMYDIAFSLGDGLRPGSIA
CCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DANDEAQFAELETLGELTQIAWKHDVQVMIEGPGHVPMHKIKENVDKQMDICKEAPFYTL
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEE
GPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHH
AADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCS
HHHHHCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHH
MCGPKFCSMRISQDIRDYAKKNDLSEAEAINKGLKEKAKEFVDTGSNLYQ
HCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MQNNSVQQANISIMSSFSGSKKVYVEGSSSDIQVPMREIALSPTTGSFGEEENAPVRVYD
CCCCCCCHHHHHHEECCCCCCEEEEECCCCCCCCCHHHHEECCCCCCCCCCCCCCEEEEE
TSGPYTDPEVTINIQEGLKPLRQKWITERGDVEEYEGRAIKPEDNGYKKAKPNVSYPGLK
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHCCCEECCCCCCCCCCCCCCCCCCCC
RKPLRAKAGQNVTQMHYAKKGIITPEMEFIAIREHVSPEFVRDEVASGRAIIPSNINHPE
CCCCHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCC
SEPMIIGRNFHVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTMMDLSTGKDIHTTREW
CCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCCHHHHHHH
IIRNCPVPVGTVPIYQALEKVNGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVP
HHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHC
LTAKRTTGIVSRGGAIMAQWCLAHHQESFLYTHFEEICEIMKMYDIAFSLGDGLRPGSIA
CCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DANDEAQFAELETLGELTQIAWKHDVQVMIEGPGHVPMHKIKENVDKQMDICKEAPFYTL
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEE
GPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRDDVREGVITYKIAAH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHH
AADLAKGHPGAQIRDDALSKARFEFRWRDQFNLSLDPERALEYHDETLPAEGAKTAHFCS
HHHHHCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHH
MCGPKFCSMRISQDIRDYAKKNDLSEAEAINKGLKEKAKEFVDTGSNLYQ
HCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA