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The map label for this gene is gsaB

Identifier: 255767197

GI number: 255767197

Start: 942449

End: 943738

Strand: Reverse

Name: gsaB

Synonym: BSU08710

Alternate gene names: 255767197

Gene position: 943738-942449 (Counterclockwise)

Preceding gene: 50812203

Following gene: 16077936

Centisome position: 22.39

GC content: 47.36

Gene sequence:

>1290_bases
TTGCATACAAAATCAATTGAACTTCACAATGAGGCATTACAGCACATTGTCGGCGGTGTAAACAGCCCGTCTAGATCATA
TAAAGCAGTCGGCGGCGGTTCTCCTGTTGCAATGGAGAAAGCAAGCGGCGCTTATTTTTGGGATGTGGACGGCAACAAAT
ACATCGATTATTTAGCGGCGTACGGTCCGATTATTACAGGACATGCCCATCCGCATATTACAGAAGCGATTAAAAAAGCA
GCAGAGAACGGTGTATTGTATGGAACACCAACGAAACACGAGGTTACATTTGCAAAAATGCTCAAAGAAGCCATTCCCGC
TATGGACAAAGTAAGATTCGTCAATTCGGGTACAGAAGCCGTTATGACGACCATCCGCGTGGCTCGCGCATATACAGGCC
GGACAAAAATCATTAAATTTGCGGGCTGCTATCACGGGCATTCGGACCTTGTGCTTGTCGCCGCAGGCTCCGGCCCGTCT
ACTCTCGGCACACCAGACTCAGCCGGTGTACCGAAAAGCATTGCAAACGAAGTGATCACAGTACCATTTAATGATATCGA
TAGCTATAAAGCAGCTCTTGAAAAATGGGGCAGTGAAATCGCGGCGGTTCTCGTTGAACCGATTGTCGGCAACTTCGGTA
TCGTGGAGCCGAAAGAGGGCTTCTTAGAACAAGTAAATGAATTAACACATAATGCAGGGGCTCTCGTTATTTACGATGAA
GTCATTACGGCTTTCCGCTTTATGTATGGCGGCGCGCAGGATCTCTTACAGGTGAAACCAGATTTAACAGCACTCGGGAA
AATTATCGGCGGCGGACTTCCGATTGGGGCCTATGGCGGCAAACAAGAAATCATGGAGCAGGTAGCTCCGCTAGGGCCTG
CTTATCAGGCAGGAACAATGGCAGGGAACCCCGCTTCCATCCTATCAGGCATCGCTTGTCTGGAAGTGCTCAAAGAAAAA
GGCGTGTATGAAAAGCTTGATCACCTTGGCGCTATGCTTGAGGAAGGCATTTTGAAGCACGCAGAAACACACGGCATCAC
CATTACGGTCAACCGGTTAAAAGGTGCGCTCACGGTATACTTCTCTGATGAGAAAGTGGAAAATTACGAGCAGGCTGAAC
GGAGTGACGGAGAAACATTCTCTACGTTTTTCAAGCTGATGCTGGAACGCGGAATTAATTTAGCACCTTCTAAGTATGAA
GCTTGGTTTATTACCACAGCACATACAGAGCAGGATATTAAAGACACGCTTACAGCGGTCGAGGATGCATTTAAACATCT
AAAAAACTAG

Upstream 100 bases:

>100_bases
TACAACCATCTCAGCTGAATCTGAAGCCCGAAGCCATTGTTCTTGTTCGGAAGCATGCTACAATAGAAAGGACGCATGGA
GATATAGGAGGTTTTTATTC

Downstream 100 bases:

>100_bases
AATCAAATACCTTGAACAGGAGCTTTGAGCTCCTGTTTTTTTATGGAATCAAACAGCGTGTAAGATTTTGGTCTTTTTCT
AGAAAAATAGCTTGAATATT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1 [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MHTKSIELHNEALQHIVGGVNSPSRSYKAVGGGSPVAMEKASGAYFWDVDGNKYIDYLAAYGPIITGHAHPHITEAIKKA
AENGVLYGTPTKHEVTFAKMLKEAIPAMDKVRFVNSGTEAVMTTIRVARAYTGRTKIIKFAGCYHGHSDLVLVAAGSGPS
TLGTPDSAGVPKSIANEVITVPFNDIDSYKAALEKWGSEIAAVLVEPIVGNFGIVEPKEGFLEQVNELTHNAGALVIYDE
VITAFRFMYGGAQDLLQVKPDLTALGKIIGGGLPIGAYGGKQEIMEQVAPLGPAYQAGTMAGNPASILSGIACLEVLKEK
GVYEKLDHLGAMLEEGILKHAETHGITITVNRLKGALTVYFSDEKVENYEQAERSDGETFSTFFKLMLERGINLAPSKYE
AWFITTAHTEQDIKDTLTAVEDAFKHLKN

Sequences:

>Translated_429_residues
MHTKSIELHNEALQHIVGGVNSPSRSYKAVGGGSPVAMEKASGAYFWDVDGNKYIDYLAAYGPIITGHAHPHITEAIKKA
AENGVLYGTPTKHEVTFAKMLKEAIPAMDKVRFVNSGTEAVMTTIRVARAYTGRTKIIKFAGCYHGHSDLVLVAAGSGPS
TLGTPDSAGVPKSIANEVITVPFNDIDSYKAALEKWGSEIAAVLVEPIVGNFGIVEPKEGFLEQVNELTHNAGALVIYDE
VITAFRFMYGGAQDLLQVKPDLTALGKIIGGGLPIGAYGGKQEIMEQVAPLGPAYQAGTMAGNPASILSGIACLEVLKEK
GVYEKLDHLGAMLEEGILKHAETHGITITVNRLKGALTVYFSDEKVENYEQAERSDGETFSTFFKLMLERGINLAPSKYE
AWFITTAHTEQDIKDTLTAVEDAFKHLKN
>Mature_429_residues
MHTKSIELHNEALQHIVGGVNSPSRSYKAVGGGSPVAMEKASGAYFWDVDGNKYIDYLAAYGPIITGHAHPHITEAIKKA
AENGVLYGTPTKHEVTFAKMLKEAIPAMDKVRFVNSGTEAVMTTIRVARAYTGRTKIIKFAGCYHGHSDLVLVAAGSGPS
TLGTPDSAGVPKSIANEVITVPFNDIDSYKAALEKWGSEIAAVLVEPIVGNFGIVEPKEGFLEQVNELTHNAGALVIYDE
VITAFRFMYGGAQDLLQVKPDLTALGKIIGGGLPIGAYGGKQEIMEQVAPLGPAYQAGTMAGNPASILSGIACLEVLKEK
GVYEKLDHLGAMLEEGILKHAETHGITITVNRLKGALTVYFSDEKVENYEQAERSDGETFSTFFKLMLERGINLAPSKYE
AWFITTAHTEQDIKDTLTAVEDAFKHLKN

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=344, Percent_Identity=32.5581395348837, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI13994255, Length=379, Percent_Identity=27.1767810026385, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI37574042, Length=355, Percent_Identity=27.6056338028169, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI24119277, Length=345, Percent_Identity=28.4057971014493, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI226442705, Length=355, Percent_Identity=27.0422535211268, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI284507298, Length=255, Percent_Identity=32.156862745098, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI226442709, Length=314, Percent_Identity=28.343949044586, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=49.2957746478873, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789016, Length=334, Percent_Identity=29.0419161676647, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI145693181, Length=381, Percent_Identity=29.9212598425197, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787560, Length=347, Percent_Identity=29.6829971181556, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1789759, Length=305, Percent_Identity=31.4754098360656, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788044, Length=293, Percent_Identity=32.0819112627986, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786991, Length=333, Percent_Identity=23.7237237237237, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144271, Length=320, Percent_Identity=29.0625, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564660, Length=362, Percent_Identity=28.1767955801105, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=372, Percent_Identity=25.2688172043011, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=305, Percent_Identity=27.8688524590164, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71981843, Length=111, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=388, Percent_Identity=27.8350515463918, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=309, Percent_Identity=24.9190938511327, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21357415, Length=356, Percent_Identity=29.4943820224719, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21356575, Length=356, Percent_Identity=27.8089887640449, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46184; Mature: 46184

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTKSIELHNEALQHIVGGVNSPSRSYKAVGGGSPVAMEKASGAYFWDVDGNKYIDYLAA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEECCCCCEEEECCCCHHHHHHHH
YGPIITGHAHPHITEAIKKAAENGVLYGTPTKHEVTFAKMLKEAIPAMDKVRFVNSGTEA
HCCEEECCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHH
VMTTIRVARAYTGRTKIIKFAGCYHGHSDLVLVAAGSGPSTLGTPDSAGVPKSIANEVIT
HHHHHHHHHHHCCCHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCEEE
VPFNDIDSYKAALEKWGSEIAAVLVEPIVGNFGIVEPKEGFLEQVNELTHNAGALVIYDE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHH
VITAFRFMYGGAQDLLQVKPDLTALGKIIGGGLPIGAYGGKQEIMEQVAPLGPAYQAGTM
HHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
AGNPASILSGIACLEVLKEKGVYEKLDHLGAMLEEGILKHAETHGITITVNRLKGALTVY
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
FSDEKVENYEQAERSDGETFSTFFKLMLERGINLAPSKYEAWFITTAHTEQDIKDTLTAV
ECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
EDAFKHLKN
HHHHHHHCC
>Mature Secondary Structure
MHTKSIELHNEALQHIVGGVNSPSRSYKAVGGGSPVAMEKASGAYFWDVDGNKYIDYLAA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEECCCCCEEEECCCCHHHHHHHH
YGPIITGHAHPHITEAIKKAAENGVLYGTPTKHEVTFAKMLKEAIPAMDKVRFVNSGTEA
HCCEEECCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHH
VMTTIRVARAYTGRTKIIKFAGCYHGHSDLVLVAAGSGPSTLGTPDSAGVPKSIANEVIT
HHHHHHHHHHHCCCHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCEEE
VPFNDIDSYKAALEKWGSEIAAVLVEPIVGNFGIVEPKEGFLEQVNELTHNAGALVIYDE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHH
VITAFRFMYGGAQDLLQVKPDLTALGKIIGGGLPIGAYGGKQEIMEQVAPLGPAYQAGTM
HHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
AGNPASILSGIACLEVLKEKGVYEKLDHLGAMLEEGILKHAETHGITITVNRLKGALTVY
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
FSDEKVENYEQAERSDGETFSTFFKLMLERGINLAPSKYEAWFITTAHTEQDIKDTLTAV
ECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
EDAFKHLKN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA