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The map label for this gene is serA [H]

Identifier: 239618528

GI number: 239618528

Start: 2300832

End: 2301755

Strand: Direct

Name: serA [H]

Synonym: Kole_2169

Alternate gene names: 239618528

Gene position: 2300832-2301755 (Clockwise)

Preceding gene: 239618527

Following gene: 239618529

Centisome position: 99.94

GC content: 44.05

Gene sequence:

>924_bases
ATGTTCAGGCTTCATGCTAATGATCCCCTGGCTCCAAAAGCAATGAATATGTTAGAGGAATCTGGACTTTTTGAAGTAAC
TCAGGAGCACCTGGACAAAGAAGAATTGCTGAAGATCATTCCAGATATCGAATTCCTCGTTGTCAGGAGTGCCACGAAGG
TCACAGCAGATATTTTGAAAGCTGGAACAAAACTCAAAGTAGTAGGAAGAGCTGGCGTAGGGCTTGACAATGTTGACGTT
TCCACTGCGAAAGAACTTGGAATCAGGGTTTACAATACACCAGGTGCAAATGCGATTTCAGCTGCTGAACTTACCATCGG
TCTTTTAATCGCTCTCATGAGGCAAATCCCCAGAGGAACCAATGGTTTGAAAGAAGGTAAGTGGGAAAAGAAGAAGCTTA
AAGGGCATGAAATTTACGGAAAAACCCTTGGACTCATAGGATTCGGTGCGATTGGCAGGGAAGTTGCAAAGAGAGCGTTA
GCTTTTGGAATGCATGTTGTTGCTTTTGATCCCTACGTTGAGAACACAGACTTAGATGTAGAACTCACCAAATCGGTTGA
CGATGTTCTTGCAAAAGCTGATGTTGTCTCGCTTCATGTTCCATTGACTCCCCGAACATTGCACATCCTCGGAGAAAAGG
AGATAGAAAAAATGAAAGATGGAGCTGTTATAATCAATGCAGCCAGAGGTGGAGTACTAGATGAACAGGCTCTTTATGAT
GCTTTAATTGCAGGAAAGTTAGCGGGTGCGGCTCTCGATGTCTTTGAGGTTGAACCGCCCGTTGATGAGCTTCGCAGAAA
GTTGCTGGGATTACCCAATGTTGTAGCCACACCTCATATTGGCGCAAGCACCTATGAAGGTCAGGAACGCGTTGGAATTG
AAATGGCCAAAAAGCTCATTGAGGTTGCAAAAGAAATAGAATAA

Upstream 100 bases:

>100_bases
TCTCCGCTCTTGAAATGGTATTGAGAAAACTCGGATACAACATAGAATACGGCTCAGGTGTGAAAGCAGCAGAAGAGATC
TTTGAGAAGGAGGGGGTTTG

Downstream 100 bases:

>100_bases
AATCTTATCAACCATTTCACTTTAGGAGGTGTTAGTGTGAAAGTTTACGTCGACAAGGACACTTGCATCGGTTGTGGCGT
TTGTGAAGGTCTCTGTCCAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MFRLHANDPLAPKAMNMLEESGLFEVTQEHLDKEELLKIIPDIEFLVVRSATKVTADILKAGTKLKVVGRAGVGLDNVDV
STAKELGIRVYNTPGANAISAAELTIGLLIALMRQIPRGTNGLKEGKWEKKKLKGHEIYGKTLGLIGFGAIGREVAKRAL
AFGMHVVAFDPYVENTDLDVELTKSVDDVLAKADVVSLHVPLTPRTLHILGEKEIEKMKDGAVIINAARGGVLDEQALYD
ALIAGKLAGAALDVFEVEPPVDELRRKLLGLPNVVATPHIGASTYEGQERVGIEMAKKLIEVAKEIE

Sequences:

>Translated_307_residues
MFRLHANDPLAPKAMNMLEESGLFEVTQEHLDKEELLKIIPDIEFLVVRSATKVTADILKAGTKLKVVGRAGVGLDNVDV
STAKELGIRVYNTPGANAISAAELTIGLLIALMRQIPRGTNGLKEGKWEKKKLKGHEIYGKTLGLIGFGAIGREVAKRAL
AFGMHVVAFDPYVENTDLDVELTKSVDDVLAKADVVSLHVPLTPRTLHILGEKEIEKMKDGAVIINAARGGVLDEQALYD
ALIAGKLAGAALDVFEVEPPVDELRRKLLGLPNVVATPHIGASTYEGQERVGIEMAKKLIEVAKEIE
>Mature_307_residues
MFRLHANDPLAPKAMNMLEESGLFEVTQEHLDKEELLKIIPDIEFLVVRSATKVTADILKAGTKLKVVGRAGVGLDNVDV
STAKELGIRVYNTPGANAISAAELTIGLLIALMRQIPRGTNGLKEGKWEKKKLKGHEIYGKTLGLIGFGAIGREVAKRAL
AFGMHVVAFDPYVENTDLDVELTKSVDDVLAKADVVSLHVPLTPRTLHILGEKEIEKMKDGAVIINAARGGVLDEQALYD
ALIAGKLAGAALDVFEVEPPVDELRRKLLGLPNVVATPHIGASTYEGQERVGIEMAKKLIEVAKEIE

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=301, Percent_Identity=46.1794019933555, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=38.4920634920635, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=38.4920634920635, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI145580575, Length=252, Percent_Identity=38.8888888888889, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4557497, Length=263, Percent_Identity=35.7414448669202, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI61743967, Length=263, Percent_Identity=35.7414448669202, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI6912396, Length=248, Percent_Identity=34.2741935483871, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1789279, Length=297, Percent_Identity=41.0774410774411, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1787645, Length=228, Percent_Identity=35.0877192982456, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI87082289, Length=260, Percent_Identity=33.4615384615385, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788660, Length=256, Percent_Identity=33.59375, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI87081824, Length=149, Percent_Identity=33.5570469798658, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=301, Percent_Identity=43.1893687707641, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25147481, Length=245, Percent_Identity=31.8367346938775, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320925, Length=303, Percent_Identity=38.9438943894389, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6322116, Length=303, Percent_Identity=38.6138613861386, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324055, Length=247, Percent_Identity=35.2226720647773, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324964, Length=302, Percent_Identity=27.8145695364238, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6325144, Length=155, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=153, Percent_Identity=30.0653594771242, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=175, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921140, Length=296, Percent_Identity=41.554054054054, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=37.1647509578544, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=37.1647509578544, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=37.1647509578544, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=37.1647509578544, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=37.1647509578544, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28574286, Length=293, Percent_Identity=34.1296928327645, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45552429, Length=295, Percent_Identity=32.5423728813559, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI28574284, Length=295, Percent_Identity=32.5423728813559, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24585514, Length=295, Percent_Identity=32.5423728813559, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI28574282, Length=295, Percent_Identity=32.5423728813559, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI45551003, Length=295, Percent_Identity=32.5423728813559, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24585516, Length=307, Percent_Identity=28.9902280130293, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571528, Length=276, Percent_Identity=32.2463768115942, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 33189; Mature: 33189

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLHANDPLAPKAMNMLEESGLFEVTQEHLDKEELLKIIPDIEFLVVRSATKVTADILK
CEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHH
AGTKLKVVGRAGVGLDNVDVSTAKELGIRVYNTPGANAISAAELTIGLLIALMRQIPRGT
CCCEEEEEECCCCCCCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
NGLKEGKWEKKKLKGHEIYGKTLGLIGFGAIGREVAKRALAFGMHVVAFDPYVENTDLDV
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCE
ELTKSVDDVLAKADVVSLHVPLTPRTLHILGEKEIEKMKDGAVIINAARGGVLDEQALYD
EEHHHHHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHCCCEEEEEECCCCCCCHHHHHH
ALIAGKLAGAALDVFEVEPPVDELRRKLLGLPNVVATPHIGASTYEGQERVGIEMAKKLI
HHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EVAKEIE
HHHHHCC
>Mature Secondary Structure
MFRLHANDPLAPKAMNMLEESGLFEVTQEHLDKEELLKIIPDIEFLVVRSATKVTADILK
CEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHH
AGTKLKVVGRAGVGLDNVDVSTAKELGIRVYNTPGANAISAAELTIGLLIALMRQIPRGT
CCCEEEEEECCCCCCCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
NGLKEGKWEKKKLKGHEIYGKTLGLIGFGAIGREVAKRALAFGMHVVAFDPYVENTDLDV
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCE
ELTKSVDDVLAKADVVSLHVPLTPRTLHILGEKEIEKMKDGAVIINAARGGVLDEQALYD
EEHHHHHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHCCCEEEEEECCCCCCCHHHHHH
ALIAGKLAGAALDVFEVEPPVDELRRKLLGLPNVVATPHIGASTYEGQERVGIEMAKKLI
HHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EVAKEIE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]