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The map label for this gene is arsB [C]

Identifier: 239618526

GI number: 239618526

Start: 2298306

End: 2299676

Strand: Direct

Name: arsB [C]

Synonym: Kole_2167

Alternate gene names: 239618526

Gene position: 2298306-2299676 (Clockwise)

Preceding gene: 239618516

Following gene: 239618527

Centisome position: 99.83

GC content: 42.01

Gene sequence:

>1371_bases
GTGGCTTTTGTCTACGGAGCAATTGTATTGTTCTTGATGGGTATTGTGTATTACTTCCTGATCAGTAACAAGATGGACAA
AGCCGCGAGCGCTTTTGCCGCTGGAGCGATCCTTCTGTTTTTGACCGCTATTTTTACAAACACTTATCCTGAACTCGTCC
CGGAGGCTATCAAGAAAATTAGTCTCGATGAGCTTAGCGAATTTGTGGACTTTAAAACCCTTGGATTGTTGCTTGGTATG
ATGATAATACTCCCTTTCATAGAAGAATCCGGATTTTTCCAGTTCGTTGCGATAACCGTTGTGAAGTTAAGCCGAGGAAA
TTTTCGCCTGCTTTTTGTGGTTACTTCGGTTATTGTTGCCATCTCTTCCGCTTTTTTGAACAATGTCAGCACCGTTATGG
TTTTTGTCCCTGTGGTTCTTGCAGTTACCGAGACCATTGGCAAGAACCCTTTCCCCTATCTCGTAATGATAATCCTTTCT
GCGAATCTCGGAGGTGCTGCAACCCTGATTGGTGATCCACCTAACATGCTTGTGGGTTTTGCAGCTGAAAAGAGTTTTAA
CGATTTTCTGGTAAATGTAGCTCCCGTAGTAGGAATAACAATCTTTACTGTGATTATTCTGCTATTGTGGAAAGAGGGAA
AATATTTTAAACTGGATGCAGAAGAAAAAGTTATTCTGAGAAGGTTTTCTCAGACCAATCCTAAAAGTCAGATTCGTGAT
AAGAAATTGATGGCAAAATCTCTGCTGGTCTTTGTTGGTGCTTTAATAGGATTCATGCTTCCACCGTCTCTGGGAATAGA
TCCGGCACTTGTATCACTCCTGGCTGCAGCTGTACTTCTCTTCTTGCTCAGGGTTGACTCCGAGACGATGGAGAAAGTGT
TTAAGAAAATAGAATGGGGAACTATTTTCTTTTTCCTTGGAATGTTCACCCTTGTCTACGGTCTGGAAGCTGTGGGGGTT
ATGGAGTATCTCGTGAATGGACTTGTTCATCTGTTTCCATCGCCGATTTTCCTGTTACTTTTTGTTCTATGGTTCCCGCT
ATTGATGGCTGGATTGGTTAGCGCTGTACCAATGGTGATGATAATGATACCAATCGTCGAAAACATAATCCATAATAGTA
GTCTTATTTTTTCAACGAACACCGGCCTGGCAGATACTTTGTGGTGGGCTCTCGTTCTTGGAGCATGTTACGGTGGAAAT
GGTACAATCGTTGGAGCAGCAGCAAATATAGTGGTAAGCGGAATGAGTCAGAAACTCGAGAGAGGAAAACTTACTTTTTC
AGAGTATTTCAGATACAGCTTCCCCATAGTCATCGTTACAGGAGTGATAGCGAGTTTGTATGTTTTATTCAGATACTATA
TTGCGCTATAA

Upstream 100 bases:

>100_bases
TTAAACCCATTCAGAGTATCGCGGAAAAACATATATGAAATTTCGTGCATGCAAGTATTTGCAAAGATGAAAATACCTTC
ATATTAGAGGAGTGGTTATA

Downstream 100 bases:

>100_bases
GGAGGTGCTTGAATTGTCAAAATTAGTAAAGAAAAATTATCTTCTCGCTCCCGGCCCTACACCCGTGCCAATTGATCTTT
TGCTGGAAGGAGCTCGGGAC

Product: Citrate transporter

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAFVYGAIVLFLMGIVYYFLISNKMDKAASAFAAGAILLFLTAIFTNTYPELVPEAIKKISLDELSEFVDFKTLGLLLGM
MIILPFIEESGFFQFVAITVVKLSRGNFRLLFVVTSVIVAISSAFLNNVSTVMVFVPVVLAVTETIGKNPFPYLVMIILS
ANLGGAATLIGDPPNMLVGFAAEKSFNDFLVNVAPVVGITIFTVIILLLWKEGKYFKLDAEEKVILRRFSQTNPKSQIRD
KKLMAKSLLVFVGALIGFMLPPSLGIDPALVSLLAAAVLLFLLRVDSETMEKVFKKIEWGTIFFFLGMFTLVYGLEAVGV
MEYLVNGLVHLFPSPIFLLLFVLWFPLLMAGLVSAVPMVMIMIPIVENIIHNSSLIFSTNTGLADTLWWALVLGACYGGN
GTIVGAAANIVVSGMSQKLERGKLTFSEYFRYSFPIVIVTGVIASLYVLFRYYIAL

Sequences:

>Translated_456_residues
MAFVYGAIVLFLMGIVYYFLISNKMDKAASAFAAGAILLFLTAIFTNTYPELVPEAIKKISLDELSEFVDFKTLGLLLGM
MIILPFIEESGFFQFVAITVVKLSRGNFRLLFVVTSVIVAISSAFLNNVSTVMVFVPVVLAVTETIGKNPFPYLVMIILS
ANLGGAATLIGDPPNMLVGFAAEKSFNDFLVNVAPVVGITIFTVIILLLWKEGKYFKLDAEEKVILRRFSQTNPKSQIRD
KKLMAKSLLVFVGALIGFMLPPSLGIDPALVSLLAAAVLLFLLRVDSETMEKVFKKIEWGTIFFFLGMFTLVYGLEAVGV
MEYLVNGLVHLFPSPIFLLLFVLWFPLLMAGLVSAVPMVMIMIPIVENIIHNSSLIFSTNTGLADTLWWALVLGACYGGN
GTIVGAAANIVVSGMSQKLERGKLTFSEYFRYSFPIVIVTGVIASLYVLFRYYIAL
>Mature_455_residues
AFVYGAIVLFLMGIVYYFLISNKMDKAASAFAAGAILLFLTAIFTNTYPELVPEAIKKISLDELSEFVDFKTLGLLLGMM
IILPFIEESGFFQFVAITVVKLSRGNFRLLFVVTSVIVAISSAFLNNVSTVMVFVPVVLAVTETIGKNPFPYLVMIILSA
NLGGAATLIGDPPNMLVGFAAEKSFNDFLVNVAPVVGITIFTVIILLLWKEGKYFKLDAEEKVILRRFSQTNPKSQIRDK
KLMAKSLLVFVGALIGFMLPPSLGIDPALVSLLAAAVLLFLLRVDSETMEKVFKKIEWGTIFFFLGMFTLVYGLEAVGVM
EYLVNGLVHLFPSPIFLLLFVLWFPLLMAGLVSAVPMVMIMIPIVENIIHNSSLIFSTNTGLADTLWWALVLGACYGGNG
TIVGAAANIVVSGMSQKLERGKLTFSEYFRYSFPIVIVTGVIASLYVLFRYYIAL

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

Organism=Homo sapiens, GI157266326, Length=472, Percent_Identity=29.6610169491525, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI87082277, Length=433, Percent_Identity=25.8660508083141, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24581759, Length=457, Percent_Identity=28.8840262582057, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28574683, Length=457, Percent_Identity=28.8840262582057, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28574681, Length=457, Percent_Identity=28.8840262582057, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28574685, Length=457, Percent_Identity=28.8840262582057, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28574678, Length=457, Percent_Identity=28.8840262582057, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920704, Length=457, Percent_Identity=28.8840262582057, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45550608, Length=450, Percent_Identity=27.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24656008, Length=452, Percent_Identity=24.5575221238938, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI85724998, Length=450, Percent_Identity=24, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI62484377, Length=450, Percent_Identity=24, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI221472711, Length=180, Percent_Identity=30.5555555555556, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24581764, Length=180, Percent_Identity=30, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 50099; Mature: 49968

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFVYGAIVLFLMGIVYYFLISNKMDKAASAFAAGAILLFLTAIFTNTYPELVPEAIKKI
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
SLDELSEFVDFKTLGLLLGMMIILPFIEESGFFQFVAITVVKLSRGNFRLLFVVTSVIVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHH
ISSAFLNNVSTVMVFVPVVLAVTETIGKNPFPYLVMIILSANLGGAATLIGDPPNMLVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHEEH
AAEKSFNDFLVNVAPVVGITIFTVIILLLWKEGKYFKLDAEEKVILRRFSQTNPKSQIRD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCHHHHHH
KKLMAKSLLVFVGALIGFMLPPSLGIDPALVSLLAAAVLLFLLRVDSETMEKVFKKIEWG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TIFFFLGMFTLVYGLEAVGVMEYLVNGLVHLFPSPIFLLLFVLWFPLLMAGLVSAVPMVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IMIPIVENIIHNSSLIFSTNTGLADTLWWALVLGACYGGNGTIVGAAANIVVSGMSQKLE
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
RGKLTFSEYFRYSFPIVIVTGVIASLYVLFRYYIAL
HCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AFVYGAIVLFLMGIVYYFLISNKMDKAASAFAAGAILLFLTAIFTNTYPELVPEAIKKI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC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HCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]