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The map label for this gene is nth [H]

Identifier: 239618511

GI number: 239618511

Start: 2281895

End: 2282527

Strand: Direct

Name: nth [H]

Synonym: Kole_2152

Alternate gene names: 239618511

Gene position: 2281895-2282527 (Clockwise)

Preceding gene: 239618510

Following gene: 239618514

Centisome position: 99.12

GC content: 44.08

Gene sequence:

>633_bases
ATGAATAATTGTAAACCGGAAGTTGTAGCGGCAAAAATAATCGAGCTTTTTCCGCGGATTCATCAGGACAGCGATCCTTA
CAGGGTCCTGGTTTCCACTGTGTTGAGTCAGAGGACGCGGGATGAAAACACAGAGGTGGCATCAAAAAAACTTTTCTCGG
TCTACCCTGATGTTTTTGCTATAGCAAAAGCAAAACCTGAGGATCTTTACAATCTGATTAAAGCGGCCGGGATGTATCGA
CAAAAGGCAGAAAGGATAGTCGAAATCTCAAAGATTATTGTTGAGACATACAACGGGAAAGTTCCGGATACCCTTGAAGA
ATTAACCAAATTGCCCGGAGTAGGAAGGAAAACCGCGAATATCGTTCTCAACGTTTCTTTTGGGAAAGCAGCACTTGCTG
TTGATACACATGTTCATAGGATCTCAAACAGGCTCGGGTGGATAAAGACAAAACAGCCGGAACAGTCTGAATTTGAGCTT
CAGAAGATTTTACCGGAGGAATTGTGGGGACCTTTGAATGGTTCTATGGTGGAATTCGGCCGAAGGGTGTGCAAGCCTGT
AAATCCACAATGCAATGAGTGTCCCATCAATTCCTGCTGCCGGTATTTCTCAGATGTTCAGAAAAAGAGGTGA

Upstream 100 bases:

>100_bases
GGCCCGGCGGTTCTTGGAATGCATTCACCCTTTGAAATAATCTCAAAAGCTGATCTCTATGAAACATACAGAGCGTACAA
AGCCTTTCTGGAGGGAAAGG

Downstream 100 bases:

>100_bases
AGAAACCGCCTATCAGTGCCAGTGAGACCAGAACGACAATCACCAGCAAAGAGCTCAGAAATCTCGCTTCGATGAGATGG
CGGCTGGACATCAACCGATA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNNCKPEVVAAKIIELFPRIHQDSDPYRVLVSTVLSQRTRDENTEVASKKLFSVYPDVFAIAKAKPEDLYNLIKAAGMYR
QKAERIVEISKIIVETYNGKVPDTLEELTKLPGVGRKTANIVLNVSFGKAALAVDTHVHRISNRLGWIKTKQPEQSEFEL
QKILPEELWGPLNGSMVEFGRRVCKPVNPQCNECPINSCCRYFSDVQKKR

Sequences:

>Translated_210_residues
MNNCKPEVVAAKIIELFPRIHQDSDPYRVLVSTVLSQRTRDENTEVASKKLFSVYPDVFAIAKAKPEDLYNLIKAAGMYR
QKAERIVEISKIIVETYNGKVPDTLEELTKLPGVGRKTANIVLNVSFGKAALAVDTHVHRISNRLGWIKTKQPEQSEFEL
QKILPEELWGPLNGSMVEFGRRVCKPVNPQCNECPINSCCRYFSDVQKKR
>Mature_210_residues
MNNCKPEVVAAKIIELFPRIHQDSDPYRVLVSTVLSQRTRDENTEVASKKLFSVYPDVFAIAKAKPEDLYNLIKAAGMYR
QKAERIVEISKIIVETYNGKVPDTLEELTKLPGVGRKTANIVLNVSFGKAALAVDTHVHRISNRLGWIKTKQPEQSEFEL
QKILPEELWGPLNGSMVEFGRRVCKPVNPQCNECPINSCCRYFSDVQKKR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=40.6779661016949, Blast_Score=141, Evalue=4e-34,
Organism=Homo sapiens, GI190358497, Length=201, Percent_Identity=29.3532338308458, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI115298654, Length=201, Percent_Identity=29.3532338308458, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI115298652, Length=201, Percent_Identity=29.3532338308458, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI115298650, Length=201, Percent_Identity=29.3532338308458, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI115298648, Length=201, Percent_Identity=29.3532338308458, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI6912520, Length=201, Percent_Identity=29.3532338308458, Blast_Score=88, Evalue=5e-18,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=35.1485148514851, Blast_Score=130, Evalue=4e-32,
Organism=Escherichia coli, GI1789331, Length=183, Percent_Identity=28.9617486338798, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554540, Length=186, Percent_Identity=40.8602150537634, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324530, Length=186, Percent_Identity=31.7204301075269, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=36.7231638418079, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23832; Mature: 23832

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNCKPEVVAAKIIELFPRIHQDSDPYRVLVSTVLSQRTRDENTEVASKKLFSVYPDVFA
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IAKAKPEDLYNLIKAAGMYRQKAERIVEISKIIVETYNGKVPDTLEELTKLPGVGRKTAN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEE
IVLNVSFGKAALAVDTHVHRISNRLGWIKTKQPEQSEFELQKILPEELWGPLNGSMVEFG
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHCCCCCCHHHHHH
RRVCKPVNPQCNECPINSCCRYFSDVQKKR
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNCKPEVVAAKIIELFPRIHQDSDPYRVLVSTVLSQRTRDENTEVASKKLFSVYPDVFA
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IAKAKPEDLYNLIKAAGMYRQKAERIVEISKIIVETYNGKVPDTLEELTKLPGVGRKTAN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEE
IVLNVSFGKAALAVDTHVHRISNRLGWIKTKQPEQSEFELQKILPEELWGPLNGSMVEFG
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHCCCCCCHHHHHH
RRVCKPVNPQCNECPINSCCRYFSDVQKKR
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]