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The map label for this gene is folA [H]

Identifier: 239618396

GI number: 239618396

Start: 2162978

End: 2163517

Strand: Reverse

Name: folA [H]

Synonym: Kole_2036

Alternate gene names: 239618396

Gene position: 2163517-2162978 (Counterclockwise)

Preceding gene: 239618397

Following gene: 239618388

Centisome position: 93.98

GC content: 35.74

Gene sequence:

>540_bases
ATGAAAATCAGTATTGTCGCTGTTAATTCAGTCAATGGTCTTATTTCAAGAGATTCCGATTACGATGTAGATTGGTCATC
CCCGGATGACAGGAAGTTCTTCAAGAAAATTACTACATCCGCTGGAGTCGTGATTTTCGGAAGACGAACCTTCGAAACTA
TTGGTAAACCTCTGCCGAAAAGATTGAATGTCATTCTCACAAGAACTCCCGAGAAATTTCATTCAACTGAAAATCTTTTA
TTTACCAACGATGAACCTGAAATCCTGATCAGCAAGATTGGAAGACTTGGTTTCAAACATATAGTCATAGGTGGAGGAAG
AGAGATATATACGCTTTTTTTGAAAAACAAACTCGTTACAGACATCTATCTTTCAATAGAACCTATAATATTAAAGGGAA
AAGTAACTATGCTAAACGTCGAAGAACTTAACAACGACATAAGATTGGAACTAGAAGAACTCTTAAAACTCTCGAAGAAT
ACATTGGTTGTTCATTATACAATCCCTGAAAATAGAGTAAGAGAGCTATTTTCACCTTGA

Upstream 100 bases:

>100_bases
AACTAAACGGATCGATAAAACTTCTTCTCGAAGAAGAGATGGACGTTATTTTCCCGCGTTCGGAAGCCATAGTTCGTCAT
ATAGTGGCGGAGGAGAAAGA

Downstream 100 bases:

>100_bases
CAATGTGATATCCCGGGTGCTAAAATCTCTTTGTGTGCGGGTGTAGCTCAGTTGGTAGAGCATCAGCTTCCCAAGCTGAG
GGTCGCGGGTTCGAGTCCCG

Product: FolA

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKISIVAVNSVNGLISRDSDYDVDWSSPDDRKFFKKITTSAGVVIFGRRTFETIGKPLPKRLNVILTRTPEKFHSTENLL
FTNDEPEILISKIGRLGFKHIVIGGGREIYTLFLKNKLVTDIYLSIEPIILKGKVTMLNVEELNNDIRLELEELLKLSKN
TLVVHYTIPENRVRELFSP

Sequences:

>Translated_179_residues
MKISIVAVNSVNGLISRDSDYDVDWSSPDDRKFFKKITTSAGVVIFGRRTFETIGKPLPKRLNVILTRTPEKFHSTENLL
FTNDEPEILISKIGRLGFKHIVIGGGREIYTLFLKNKLVTDIYLSIEPIILKGKVTMLNVEELNNDIRLELEELLKLSKN
TLVVHYTIPENRVRELFSP
>Mature_179_residues
MKISIVAVNSVNGLISRDSDYDVDWSSPDDRKFFKKITTSAGVVIFGRRTFETIGKPLPKRLNVILTRTPEKFHSTENLL
FTNDEPEILISKIGRLGFKHIVIGGGREIYTLFLKNKLVTDIYLSIEPIILKGKVTMLNVEELNNDIRLELEELLKLSKN
TLVVHYTIPENRVRELFSP

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20471; Mature: 20471

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISIVAVNSVNGLISRDSDYDVDWSSPDDRKFFKKITTSAGVVIFGRRTFETIGKPLPK
CEEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHCCCCCC
RLNVILTRTPEKFHSTENLLFTNDEPEILISKIGRLGFKHIVIGGGREIYTLFLKNKLVT
CEEEEEEECCHHHCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEE
DIYLSIEPIILKGKVTMLNVEELNNDIRLELEELLKLSKNTLVVHYTIPENRVRELFSP
EEEEEEEEEEEECEEEEEEHHHCCCCEEEEHHHHHHCCCCEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MKISIVAVNSVNGLISRDSDYDVDWSSPDDRKFFKKITTSAGVVIFGRRTFETIGKPLPK
CEEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHCCCCCC
RLNVILTRTPEKFHSTENLLFTNDEPEILISKIGRLGFKHIVIGGGREIYTLFLKNKLVT
CEEEEEEECCHHHCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEE
DIYLSIEPIILKGKVTMLNVEELNNDIRLELEELLKLSKNTLVVHYTIPENRVRELFSP
EEEEEEEEEEEECEEEEEEHHHCCCCEEEEHHHHHHCCCCEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7789791; 9563834; 10360571; 10731419 [H]