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The map label for this gene is gltX [H]

Identifier: 239618313

GI number: 239618313

Start: 2067892

End: 2069319

Strand: Reverse

Name: gltX [H]

Synonym: Kole_1950

Alternate gene names: 239618313

Gene position: 2069319-2067892 (Counterclockwise)

Preceding gene: 239618314

Following gene: 239618312

Centisome position: 89.89

GC content: 43.42

Gene sequence:

>1428_bases
ATGGTAAGATGTAGATTTGCACCGAGCCCCACAGGACATATGCACGTGGGTGGCGTAAGGACCGCTCTTTTCAACTGGCT
TTTCGCAAGAAATCAGGGTGGAAGTTTTATTTTGAGAATCGAGGATACCGACACAGAAAGGTCCACTAGAGAATCTGAAG
AACAGATAATCAACTCCCTGCGGTGGTGTGGTCTTAACTGGGATGAAGGCCCGGACATAGGTGGAAAGTACGGTCCTTAC
AGACAGAGTGAACGCACCAAAGCGGGATTGTATAAAAGGTATGCGGATGAACTGGTAAAGAACAATCACGCATACTATGC
TATATATCACAAAGACGATCCTAAAACAGTTATTAAAACCACTACGGAAGAACCCACAGATCTTGGAGAAGAATACACAT
ATACTGTTAACTTCAAGGTTCCAAAGGAAGGCGAAACCACCTTTGAAGATCTTCTCAAAGGCAACATGGTTTTTAAAAAC
GAGACCTTTGACGACTTCGTTATAATAAAATCCAACGGGTTCCCTACCTATAATTTTGCCGTGGTGATAGATGACCATCT
GATGGAAATCACCCATGTCTTCCGTGGAGAAGACCATCTCTCGAACACTCCGAAACAACTGATGATTTACGACGCCTTCG
GCTGGAAAGCGCCGACGTTTATGCATATACCTCTTATACTCGGCAATGATAGAAGTCCGTTATCTAAAAGGCATGGACAC
ACGAGCGTCGATTACTTCAGGAGAGAAGGCTATTTGAGCACTGCCCTTATGAATTATCTTGCACTTCTGGGATGGAGTGT
AGATGAAGAGATCTTTGATTTTAGAGATAAAATCAGGGACTTTGATCCCCGTTCCATATCCAATAAAGCCGTTATTTTTG
ACTATGAGAAGCTTGAATGGATCAACGGTAAGCACCTCAGAGTGATCCCATTGGAGGAACTCATGACCGAGTTCGAGCTC
TGGTTGAGATACACTGAAAAATTCGATTTTTATGAGAAACTACTGAAAGATCCTGAGTACAGCAAACAGGTTATTTCGAT
CTGTCGCGAAAAGATAAATACCATGTCGCAGCTCCTTGATTTTTCTGCTCCTTTCTTTTCAGATGAATTGGAATATGAAA
GCAGATTTGTGGATAAATATCTCAAAAAAAGCTGGAGCACAGAGGTTTTAGAAGCCGCCATAAGGCGTTTTGAAGAGAAC
GATGATTGGTCTATTGAAGGCGTGGAGAAGGTTATCCGGGACGTGAGTGAAGAGAAGCACACTTCCAAGAAAAACACCTT
CCAGACAATTCGAGGAGCCGTAACAGGCAGATTGGTCACTCCGGGACTTTTTGAAACCGTTGCTGTGCTTGGGAAAGAGA
AAACATTGGAAAGGCTCGAGGCAACTCTTGACCTCGCAGAAAGCTTGAAGGATGAAGAAGGGATTTAG

Upstream 100 bases:

>100_bases
CCTAATAGACGGGTATTATCTGGATAATCGCCGCCTGACAAAGATCCTTCAAATATCCTCAATAGAAGAATTGATGCTAC
ACATCTAAAGGAGGAATACC

Downstream 100 bases:

>100_bases
TCTCGTTGGAATTTTGATGTCTCTGGTGGCGACGATTTTTATCGTTGCTGTCGTTTTTTCTGTCTTCACAGAATTCAATC
TCGTGAATTCGCAATACATG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2 [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MVRCRFAPSPTGHMHVGGVRTALFNWLFARNQGGSFILRIEDTDTERSTRESEEQIINSLRWCGLNWDEGPDIGGKYGPY
RQSERTKAGLYKRYADELVKNNHAYYAIYHKDDPKTVIKTTTEEPTDLGEEYTYTVNFKVPKEGETTFEDLLKGNMVFKN
ETFDDFVIIKSNGFPTYNFAVVIDDHLMEITHVFRGEDHLSNTPKQLMIYDAFGWKAPTFMHIPLILGNDRSPLSKRHGH
TSVDYFRREGYLSTALMNYLALLGWSVDEEIFDFRDKIRDFDPRSISNKAVIFDYEKLEWINGKHLRVIPLEELMTEFEL
WLRYTEKFDFYEKLLKDPEYSKQVISICREKINTMSQLLDFSAPFFSDELEYESRFVDKYLKKSWSTEVLEAAIRRFEEN
DDWSIEGVEKVIRDVSEEKHTSKKNTFQTIRGAVTGRLVTPGLFETVAVLGKEKTLERLEATLDLAESLKDEEGI

Sequences:

>Translated_475_residues
MVRCRFAPSPTGHMHVGGVRTALFNWLFARNQGGSFILRIEDTDTERSTRESEEQIINSLRWCGLNWDEGPDIGGKYGPY
RQSERTKAGLYKRYADELVKNNHAYYAIYHKDDPKTVIKTTTEEPTDLGEEYTYTVNFKVPKEGETTFEDLLKGNMVFKN
ETFDDFVIIKSNGFPTYNFAVVIDDHLMEITHVFRGEDHLSNTPKQLMIYDAFGWKAPTFMHIPLILGNDRSPLSKRHGH
TSVDYFRREGYLSTALMNYLALLGWSVDEEIFDFRDKIRDFDPRSISNKAVIFDYEKLEWINGKHLRVIPLEELMTEFEL
WLRYTEKFDFYEKLLKDPEYSKQVISICREKINTMSQLLDFSAPFFSDELEYESRFVDKYLKKSWSTEVLEAAIRRFEEN
DDWSIEGVEKVIRDVSEEKHTSKKNTFQTIRGAVTGRLVTPGLFETVAVLGKEKTLERLEATLDLAESLKDEEGI
>Mature_475_residues
MVRCRFAPSPTGHMHVGGVRTALFNWLFARNQGGSFILRIEDTDTERSTRESEEQIINSLRWCGLNWDEGPDIGGKYGPY
RQSERTKAGLYKRYADELVKNNHAYYAIYHKDDPKTVIKTTTEEPTDLGEEYTYTVNFKVPKEGETTFEDLLKGNMVFKN
ETFDDFVIIKSNGFPTYNFAVVIDDHLMEITHVFRGEDHLSNTPKQLMIYDAFGWKAPTFMHIPLILGNDRSPLSKRHGH
TSVDYFRREGYLSTALMNYLALLGWSVDEEIFDFRDKIRDFDPRSISNKAVIFDYEKLEWINGKHLRVIPLEELMTEFEL
WLRYTEKFDFYEKLLKDPEYSKQVISICREKINTMSQLLDFSAPFFSDELEYESRFVDKYLKKSWSTEVLEAAIRRFEEN
DDWSIEGVEKVIRDVSEEKHTSKKNTFQTIRGAVTGRLVTPGLFETVAVLGKEKTLERLEATLDLAESLKDEEGI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=509, Percent_Identity=29.4695481335953, Blast_Score=218, Evalue=8e-57,
Organism=Escherichia coli, GI1788743, Length=490, Percent_Identity=35.5102040816327, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI226510925, Length=272, Percent_Identity=30.1470588235294, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=321, Percent_Identity=38.006230529595, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=512, Percent_Identity=32.8125, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24665321, Length=506, Percent_Identity=33.201581027668, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55396; Mature: 55396

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRCRFAPSPTGHMHVGGVRTALFNWLFARNQGGSFILRIEDTDTERSTRESEEQIINSL
CEEEECCCCCCCCEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
RWCGLNWDEGPDIGGKYGPYRQSERTKAGLYKRYADELVKNNHAYYAIYHKDDPKTVIKT
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEE
TTEEPTDLGEEYTYTVNFKVPKEGETTFEDLLKGNMVFKNETFDDFVIIKSNGFPTYNFA
CCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCCEEEECCCCCCEEEEECCCCCCEEEE
VVIDDHLMEITHVFRGEDHLSNTPKQLMIYDAFGWKAPTFMHIPLILGNDRSPLSKRHGH
EEECHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHCCC
TSVDYFRREGYLSTALMNYLALLGWSVDEEIFDFRDKIRDFDPRSISNKAVIFDYEKLEW
CHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHC
INGKHLRVIPLEELMTEFELWLRYTEKFDFYEKLLKDPEYSKQVISICREKINTMSQLLD
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FSAPFFSDELEYESRFVDKYLKKSWSTEVLEAAIRRFEENDDWSIEGVEKVIRDVSEEKH
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TSKKNTFQTIRGAVTGRLVTPGLFETVAVLGKEKTLERLEATLDLAESLKDEEGI
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVRCRFAPSPTGHMHVGGVRTALFNWLFARNQGGSFILRIEDTDTERSTRESEEQIINSL
CEEEECCCCCCCCEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
RWCGLNWDEGPDIGGKYGPYRQSERTKAGLYKRYADELVKNNHAYYAIYHKDDPKTVIKT
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEE
TTEEPTDLGEEYTYTVNFKVPKEGETTFEDLLKGNMVFKNETFDDFVIIKSNGFPTYNFA
CCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCCEEEECCCCCCEEEEECCCCCCEEEE
VVIDDHLMEITHVFRGEDHLSNTPKQLMIYDAFGWKAPTFMHIPLILGNDRSPLSKRHGH
EEECHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHCCC
TSVDYFRREGYLSTALMNYLALLGWSVDEEIFDFRDKIRDFDPRSISNKAVIFDYEKLEW
CHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHC
INGKHLRVIPLEELMTEFELWLRYTEKFDFYEKLLKDPEYSKQVISICREKINTMSQLLD
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FSAPFFSDELEYESRFVDKYLKKSWSTEVLEAAIRRFEENDDWSIEGVEKVIRDVSEEKH
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TSKKNTFQTIRGAVTGRLVTPGLFETVAVLGKEKTLERLEATLDLAESLKDEEGI
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]