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The map label for this gene is truA

Identifier: 239618279

GI number: 239618279

Start: 2038830

End: 2039582

Strand: Reverse

Name: truA

Synonym: Kole_1916

Alternate gene names: 239618279

Gene position: 2039582-2038830 (Counterclockwise)

Preceding gene: 239618280

Following gene: 239618278

Centisome position: 88.6

GC content: 40.9

Gene sequence:

>753_bases
ATGAAGCGATTTGCAGCGGTTGTAGTTTACGATGGCAGTAACTTTTTCGGATACCAGATTCAACCAAACAAAAGGACAGT
TCAAGGGGTTTTTGAAGAAGCCCTTGAGCGAATACATAAAGCAAAAATTCCAACTGATGGTGCAGGTAGAACCGATACGG
GCGTACATGCTTACGGTCAGGTAATAGCCTTCACCTCGAACCTTGACCGGCTAACCCCCAAACATATGAAAGATGCTTTA
AATGCTAATCTTCCAGATGACATATATGTACGGAGAATTCATGAAGTCCCGTCGAACTTCAGTCCAAGGTTCGCGGCAAA
AAAGAGGATTTACCACTATTACATACTGAATACGAAAGAACCGGATCTTTTTCGAAGGAAGTATTTCTGGTGGTTTCCGT
ATGAGGTAGACTTAAATCGTATGCGTCAGGCGTCAAGATATCTGATCGGTGAACATGATTTTACATCCTTCAGAACCGGT
CATGACGACAGAAGCACCATAAGAACAATAACAGGAATAAGGATTTTAAGGCTACAGAAAAACATCATTTTAATAAGAGT
TGAAGGTATTTCCTTCCTCAGGAGAATGGTGAGAAATATAGTAGGTCTTTTAATGAGAGTTGGAACTGGTGGAATTGAAC
CGAAAAAAACAAAAGAAATCCTTGAAGCACGGGACAGATCAAAACTTCCTAGTTCAGCTCCTGCTCATGGCTTGGTGCTT
CACAAGGTGATTTTTGATGAGTTTGAAACTTAG

Upstream 100 bases:

>100_bases
CTACGGAGGGGATTTGAACCGTTTGAGAAAGGATTTATCCGCCATAGTTTACACCTATACCGAAATGAAGGATTTCTTTG
AAGGGGTCTGAGGTGAAGTC

Downstream 100 bases:

>100_bases
ACTTGATGAATTGCTTGTAGCAAGAAACTTTTTCTCGAGCCGTTCGAGAGCCAGCGATATAATCAAACAGGGGAAGGTTT
CGATTAACGGCAAGATTGTT

Product: tRNA pseudouridine synthase ACD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKRFAAVVVYDGSNFFGYQIQPNKRTVQGVFEEALERIHKAKIPTDGAGRTDTGVHAYGQVIAFTSNLDRLTPKHMKDAL
NANLPDDIYVRRIHEVPSNFSPRFAAKKRIYHYYILNTKEPDLFRRKYFWWFPYEVDLNRMRQASRYLIGEHDFTSFRTG
HDDRSTIRTITGIRILRLQKNIILIRVEGISFLRRMVRNIVGLLMRVGTGGIEPKKTKEILEARDRSKLPSSAPAHGLVL
HKVIFDEFET

Sequences:

>Translated_250_residues
MKRFAAVVVYDGSNFFGYQIQPNKRTVQGVFEEALERIHKAKIPTDGAGRTDTGVHAYGQVIAFTSNLDRLTPKHMKDAL
NANLPDDIYVRRIHEVPSNFSPRFAAKKRIYHYYILNTKEPDLFRRKYFWWFPYEVDLNRMRQASRYLIGEHDFTSFRTG
HDDRSTIRTITGIRILRLQKNIILIRVEGISFLRRMVRNIVGLLMRVGTGGIEPKKTKEILEARDRSKLPSSAPAHGLVL
HKVIFDEFET
>Mature_250_residues
MKRFAAVVVYDGSNFFGYQIQPNKRTVQGVFEEALERIHKAKIPTDGAGRTDTGVHAYGQVIAFTSNLDRLTPKHMKDAL
NANLPDDIYVRRIHEVPSNFSPRFAAKKRIYHYYILNTKEPDLFRRKYFWWFPYEVDLNRMRQASRYLIGEHDFTSFRTG
HDDRSTIRTITGIRILRLQKNIILIRVEGISFLRRMVRNIVGLLMRVGTGGIEPKKTKEILEARDRSKLPSSAPAHGLVL
HKVIFDEFET

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=30.5970149253731, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=37.0517928286853, Blast_Score=144, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321112, Length=232, Percent_Identity=28.8793103448276, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_KOSOT (C5CGS8)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941601.1
- GeneID:   7968407
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1916
- ProtClustDB:   PRK14586
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29066; Mature: 29066

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFAAVVVYDGSNFFGYQIQPNKRTVQGVFEEALERIHKAKIPTDGAGRTDTGVHAYGQ
CCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
VIAFTSNLDRLTPKHMKDALNANLPDDIYVRRIHEVPSNFSPRFAAKKRIYHYYILNTKE
EEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHEEEEEEEEECCC
PDLFRRKYFWWFPYEVDLNRMRQASRYLIGEHDFTSFRTGHDDRSTIRTITGIRILRLQK
CHHHHHEEEEEECEEECHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEEC
NIILIRVEGISFLRRMVRNIVGLLMRVGTGGIEPKKTKEILEARDRSKLPSSAPAHGLVL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
HKVIFDEFET
HHHHHHHCCC
>Mature Secondary Structure
MKRFAAVVVYDGSNFFGYQIQPNKRTVQGVFEEALERIHKAKIPTDGAGRTDTGVHAYGQ
CCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
VIAFTSNLDRLTPKHMKDALNANLPDDIYVRRIHEVPSNFSPRFAAKKRIYHYYILNTKE
EEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHEEEEEEEEECCC
PDLFRRKYFWWFPYEVDLNRMRQASRYLIGEHDFTSFRTGHDDRSTIRTITGIRILRLQK
CHHHHHEEEEEECEEECHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEEC
NIILIRVEGISFLRRMVRNIVGLLMRVGTGGIEPKKTKEILEARDRSKLPSSAPAHGLVL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
HKVIFDEFET
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA