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The map label for this gene is rpoA [H]

Identifier: 239618237

GI number: 239618237

Start: 2009600

End: 2010640

Strand: Reverse

Name: rpoA [H]

Synonym: Kole_1874

Alternate gene names: 239618237

Gene position: 2010640-2009600 (Counterclockwise)

Preceding gene: 239618238

Following gene: 239618236

Centisome position: 87.34

GC content: 39.67

Gene sequence:

>1041_bases
GTGCCCGATACTTGGGCAGTCACAATGGTTGGATTTGTAATGCCAAAATCTCTAAAGATTGAAGAGGAGAGAGAGGAACA
GAACAGTTATTACGCAAAGTTTGTCCTTTCACCAATGGAAAGAGGATACGCTGTAACCATAGGAAATGCGCTCAGAAGAG
TTCTCCTCTCCTCAATTCCAAGTATGGCAATAACCAGATTGAAAATCCCGGGGAAATATCATGAGTATGATACAATCGAT
GGGGTTAAAGAGGACATCCTTGAAATAATCCTGAATTTAAAAAAAGTGCAGCTTAAACCTGCCCTAGAGTTTTCTGATCC
TGTTAAGCTAAGCATAGAGAAAAAGGGCCCAGCTATTTTAACTGCTGGAGATATAAAAGCTCCTACAGGAGTCGAAGTGG
TTAACCCTTCCCAATATATAGCCACCTTAAATGAAGATGCTGCTGTTTACATGGAGCTCTTTGCTGAAATTGGTAAGGGT
TTCGTTCCAGTCTCGGAAATGGAGCTGGAACAGGATGTGGAAATGATCTATATAGATGGTATATTCAGTCCGGTTCTTAA
AGTCAATTTCATTTCCGAAAATGTTCGTGTAGGAAAGAAAACCGACTACGACAAACTCATTCTGGAAGTCTGGACAAAGA
AATCTATAAAACCAACAGAAGCGCTTTTAAAGGCAACAGATATATTGATAAAGCATTTTGAGGTTACCACATCAAGTCTG
AATCAGGTACTTGATAATTCAGGAATTTTAGTAAACAGCGCTTCGATTTTTGAACCCGAAGAACCCCAACCGGAAGAAGA
ACCTGTTGAAGAACCCATCTCAGAAGAGGATTCCGTGCTTTCAAGACGTATAGAAGAACTGGAACTATCCGTAAGGTCGC
TTAACTGTCTGAAGCGCGATAAAATCAACACAATTGGTGACCTTATAAGCAGAACAGAGGCTGATCTTCTTAAAATAAGG
AACTTTGGTGATAAATCAATGGAAGAAGTCAAGAAAAAATTGAAGGAGAAGTTCAACCTCTCACTAAAGAAAGAACAGTA
A

Upstream 100 bases:

>100_bases
TAGATTCCCTGAGAGAGAAGAAGTAGATATTCCTGTCGATCTGCAATCCATCATCGAGCTCTACTCGAAGTAATGTTGTT
ACCTTCTTGAAAGGAGGTGT

Downstream 100 bases:

>100_bases
AAGAAGGTTAAGGAGGTTGCCCCATGAGACATAGAGTCAAGAAGAAAAAGCTCAACAGACCTCACAGCCAAAGGCTCGCA
TTAATGCGAGGTCTTGCGAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPDTWAVTMVGFVMPKSLKIEEEREEQNSYYAKFVLSPMERGYAVTIGNALRRVLLSSIPSMAITRLKIPGKYHEYDTID
GVKEDILEIILNLKKVQLKPALEFSDPVKLSIEKKGPAILTAGDIKAPTGVEVVNPSQYIATLNEDAAVYMELFAEIGKG
FVPVSEMELEQDVEMIYIDGIFSPVLKVNFISENVRVGKKTDYDKLILEVWTKKSIKPTEALLKATDILIKHFEVTTSSL
NQVLDNSGILVNSASIFEPEEPQPEEEPVEEPISEEDSVLSRRIEELELSVRSLNCLKRDKINTIGDLISRTEADLLKIR
NFGDKSMEEVKKKLKEKFNLSLKKEQ

Sequences:

>Translated_346_residues
MPDTWAVTMVGFVMPKSLKIEEEREEQNSYYAKFVLSPMERGYAVTIGNALRRVLLSSIPSMAITRLKIPGKYHEYDTID
GVKEDILEIILNLKKVQLKPALEFSDPVKLSIEKKGPAILTAGDIKAPTGVEVVNPSQYIATLNEDAAVYMELFAEIGKG
FVPVSEMELEQDVEMIYIDGIFSPVLKVNFISENVRVGKKTDYDKLILEVWTKKSIKPTEALLKATDILIKHFEVTTSSL
NQVLDNSGILVNSASIFEPEEPQPEEEPVEEPISEEDSVLSRRIEELELSVRSLNCLKRDKINTIGDLISRTEADLLKIR
NFGDKSMEEVKKKLKEKFNLSLKKEQ
>Mature_345_residues
PDTWAVTMVGFVMPKSLKIEEEREEQNSYYAKFVLSPMERGYAVTIGNALRRVLLSSIPSMAITRLKIPGKYHEYDTIDG
VKEDILEIILNLKKVQLKPALEFSDPVKLSIEKKGPAILTAGDIKAPTGVEVVNPSQYIATLNEDAAVYMELFAEIGKGF
VPVSEMELEQDVEMIYIDGIFSPVLKVNFISENVRVGKKTDYDKLILEVWTKKSIKPTEALLKATDILIKHFEVTTSSLN
QVLDNSGILVNSASIFEPEEPQPEEEPVEEPISEEDSVLSRRIEELELSVRSLNCLKRDKINTIGDLISRTEADLLKIRN
FGDKSMEEVKKKLKEKFNLSLKKEQ

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=336, Percent_Identity=38.9880952380952, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 39067; Mature: 38935

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTWAVTMVGFVMPKSLKIEEEREEQNSYYAKFVLSPMERGYAVTIGNALRRVLLSSIP
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCC
SMAITRLKIPGKYHEYDTIDGVKEDILEIILNLKKVQLKPALEFSDPVKLSIEKKGPAIL
HHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCEEE
TAGDIKAPTGVEVVNPSQYIATLNEDAAVYMELFAEIGKGFVPVSEMELEQDVEMIYIDG
EECCCCCCCCCEEECCHHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECC
IFSPVLKVNFISENVRVGKKTDYDKLILEVWTKKSIKPTEALLKATDILIKHFEVTTSSL
HHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NQVLDNSGILVNSASIFEPEEPQPEEEPVEEPISEEDSVLSRRIEELELSVRSLNCLKRD
HHHHCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KINTIGDLISRTEADLLKIRNFGDKSMEEVKKKLKEKFNLSLKKEQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PDTWAVTMVGFVMPKSLKIEEEREEQNSYYAKFVLSPMERGYAVTIGNALRRVLLSSIP
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCC
SMAITRLKIPGKYHEYDTIDGVKEDILEIILNLKKVQLKPALEFSDPVKLSIEKKGPAIL
HHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCEEE
TAGDIKAPTGVEVVNPSQYIATLNEDAAVYMELFAEIGKGFVPVSEMELEQDVEMIYIDG
EECCCCCCCCCEEECCHHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECC
IFSPVLKVNFISENVRVGKKTDYDKLILEVWTKKSIKPTEALLKATDILIKHFEVTTSSL
HHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NQVLDNSGILVNSASIFEPEEPQPEEEPVEEPISEEDSVLSRRIEELELSVRSLNCLKRD
HHHHCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KINTIGDLISRTEADLLKIRNFGDKSMEEVKKKLKEKFNLSLKKEQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]