Click here to switch to the map view.

The map label for this gene is asnS [H]

Identifier: 239618190

GI number: 239618190

Start: 1954320

End: 1955264

Strand: Direct

Name: asnS [H]

Synonym: Kole_1826

Alternate gene names: 239618190

Gene position: 1954320-1955264 (Clockwise)

Preceding gene: 239618186

Following gene: 239618191

Centisome position: 84.89

GC content: 42.96

Gene sequence:

>945_bases
ATGATGAAAGGTTGTTCAGACCGTCTTGAAATGGTGAGAAGTTATTTAGTGGACGAAACTTACAGAGACGCGTTATTAGT
CCAGAGCGAACTTTTGAAAGGCTTGAGGGAAACTCTTGACGCCAAAGGTTTTGTCGAAATTCTTCCTGTTATTCTCTCTC
CCATTACCGATCCGCTGAACCACGAGGTGTTTGACGCCAGTGTGGAATACTACGGCCAGAATTATGCTGTAACGAAATCT
ATGATACTTCACAAACAGATAAGTGTTCTTGTACACGAAAAGATATACAGCGTATCGCCGAATGTTCGTCTCGAAACAGC
CGATAAGGCAGATTCAGGCCGACACCTTTTTGAATTTGTGCAGCTGGATATGGAAGTCGCCGGAGCTTCCAGGGACGAGA
TAATGGATGTAATGGAAGATGCAATAATCTATGCGATAAGAAATGTTTTGTCTAAATATCCATGGGTTAAAGAGAAATAC
CATCCGACATTGAAAGTTCCCGCAAAACCTTTCAAAAGGATAACGGTGAAAGAAGCGGTGGAAAAGTATGGAGAAGCTTT
TGAAAGTGGTCTTTCAAAGGATATTGATGAACCTGTCTGGCTCATAGACATTCCGTTGCTCAACAGAGAATTCTACGATA
AACAGGACCCGGAAAGACCGGATGTGTTGCTTGATTTTGATCTTATATATCCTGAAGGTTTCGGTGAAGGAATATCGGGT
GGAGAAAGGGAACACGAATATGAACAGATAATCAGGAGAATGAAATTAAAGGGAACGAGCCCGGAAGAATATACGGACTA
TCTTGAACTGGCAAAACAGGGGGTTTTAAAACCGTCTGCCGGTTGCGGGATAGGTATAGAAAGATTTACAAGGTACGTTA
TGGGACTTGAACACGTGCGGCAGACGAGACTTTTCGCCAAAGTTCCGGGAGAAAGCGCACTTTAA

Upstream 100 bases:

>100_bases
CGCCGGCTCCGGTGCTAAGTGCTGTTTTTGCACATAAAGAGCCGGCACCTATTGTTGGGTGCCGGCTTTTTTTATATACA
CTTAAAGGAGGCGAGGTTTA

Downstream 100 bases:

>100_bases
TCCTGCGATGAAAAAATTTTAGGAGATTCTACGATGTTTTTTCAAAACCCTCCGGGGCTGCTATTATAGGAATGAGCCCA
TAGAAAGGTGGGAAAAGTAT

Product: asparagine synthetase A

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MMKGCSDRLEMVRSYLVDETYRDALLVQSELLKGLRETLDAKGFVEILPVILSPITDPLNHEVFDASVEYYGQNYAVTKS
MILHKQISVLVHEKIYSVSPNVRLETADKADSGRHLFEFVQLDMEVAGASRDEIMDVMEDAIIYAIRNVLSKYPWVKEKY
HPTLKVPAKPFKRITVKEAVEKYGEAFESGLSKDIDEPVWLIDIPLLNREFYDKQDPERPDVLLDFDLIYPEGFGEGISG
GEREHEYEQIIRRMKLKGTSPEEYTDYLELAKQGVLKPSAGCGIGIERFTRYVMGLEHVRQTRLFAKVPGESAL

Sequences:

>Translated_314_residues
MMKGCSDRLEMVRSYLVDETYRDALLVQSELLKGLRETLDAKGFVEILPVILSPITDPLNHEVFDASVEYYGQNYAVTKS
MILHKQISVLVHEKIYSVSPNVRLETADKADSGRHLFEFVQLDMEVAGASRDEIMDVMEDAIIYAIRNVLSKYPWVKEKY
HPTLKVPAKPFKRITVKEAVEKYGEAFESGLSKDIDEPVWLIDIPLLNREFYDKQDPERPDVLLDFDLIYPEGFGEGISG
GEREHEYEQIIRRMKLKGTSPEEYTDYLELAKQGVLKPSAGCGIGIERFTRYVMGLEHVRQTRLFAKVPGESAL
>Mature_314_residues
MMKGCSDRLEMVRSYLVDETYRDALLVQSELLKGLRETLDAKGFVEILPVILSPITDPLNHEVFDASVEYYGQNYAVTKS
MILHKQISVLVHEKIYSVSPNVRLETADKADSGRHLFEFVQLDMEVAGASRDEIMDVMEDAIIYAIRNVLSKYPWVKEKY
HPTLKVPAKPFKRITVKEAVEKYGEAFESGLSKDIDEPVWLIDIPLLNREFYDKQDPERPDVLLDFDLIYPEGFGEGISG
GEREHEYEQIIRRMKLKGTSPEEYTDYLELAKQGVLKPSAGCGIGIERFTRYVMGLEHVRQTRLFAKVPGESAL

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=322, Percent_Identity=30.4347826086957, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI4758762, Length=322, Percent_Identity=22.9813664596273, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI226491502, Length=328, Percent_Identity=25, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1787161, Length=277, Percent_Identity=25.6317689530686, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=311, Percent_Identity=26.3665594855305, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17551876, Length=316, Percent_Identity=27.5316455696203, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=306, Percent_Identity=25.1633986928105, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319872, Length=322, Percent_Identity=26.3975155279503, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323011, Length=318, Percent_Identity=27.0440251572327, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921528, Length=323, Percent_Identity=25.077399380805, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17136276, Length=309, Percent_Identity=27.831715210356, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 35917; Mature: 35917

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKGCSDRLEMVRSYLVDETYRDALLVQSELLKGLRETLDAKGFVEILPVILSPITDPLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
HEVFDASVEYYGQNYAVTKSMILHKQISVLVHEKIYSVSPNVRLETADKADSGRHLFEFV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
QLDMEVAGASRDEIMDVMEDAIIYAIRNVLSKYPWVKEKYHPTLKVPAKPFKRITVKEAV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEECCCCHHHHHHHHHHH
EKYGEAFESGLSKDIDEPVWLIDIPLLNREFYDKQDPERPDVLLDFDLIYPEGFGEGISG
HHHHHHHHHHHHCCCCCCEEEEECCHHCCHHCCCCCCCCCCEEEEEEEECCCCCCCCCCC
GEREHEYEQIIRRMKLKGTSPEEYTDYLELAKQGVLKPSAGCGIGIERFTRYVMGLEHVR
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QTRLFAKVPGESAL
HHHHHHCCCCCCCC
>Mature Secondary Structure
MMKGCSDRLEMVRSYLVDETYRDALLVQSELLKGLRETLDAKGFVEILPVILSPITDPLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
HEVFDASVEYYGQNYAVTKSMILHKQISVLVHEKIYSVSPNVRLETADKADSGRHLFEFV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
QLDMEVAGASRDEIMDVMEDAIIYAIRNVLSKYPWVKEKYHPTLKVPAKPFKRITVKEAV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEECCCCHHHHHHHHHHH
EKYGEAFESGLSKDIDEPVWLIDIPLLNREFYDKQDPERPDVLLDFDLIYPEGFGEGISG
HHHHHHHHHHHHCCCCCCEEEEECCHHCCHHCCCCCCCCCCEEEEEEEECCCCCCCCCCC
GEREHEYEQIIRRMKLKGTSPEEYTDYLELAKQGVLKPSAGCGIGIERFTRYVMGLEHVR
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QTRLFAKVPGESAL
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA