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The map label for this gene is panB

Identifier: 239618092

GI number: 239618092

Start: 1852960

End: 1853763

Strand: Direct

Name: panB

Synonym: Kole_1724

Alternate gene names: 239618092

Gene position: 1852960-1853763 (Clockwise)

Preceding gene: 239618091

Following gene: 239618093

Centisome position: 80.49

GC content: 46.52

Gene sequence:

>804_bases
TTGAACATAAGGCAGATAATTAATTCAAAGGGCAAGCGAAAGCTTTCCATGGTGACAGCTTACAGTTACTTTCAGGCTAA
GATGGCAGAAGAAGCCGGTATAGATATGATTCTTGTTGGAGATTCTTATGGTAATACTATACTCGGTTATGAAAATACCT
TACCGGTGACAATGGAGGAGATGCTTATCGCCGTTTCGGCCGTGAGAAGGGGTGCGCCGAACACCTTTGTTATAGCGGAT
ATGCCCTTTTTGTCTTACCAGGTTTCTGAAGAAAAGGCGATAGAAAACGCCGGGCGTTTTATTAAGGTTGGTGCAAACGC
TGTGAAGCTTGAAGGCGGCGCCGAGGTTGCGGGGCTTGTGAAGAAACTGGTGGATTTTGGCATACCAGTGATGGGGCACC
TTGGGTTGACACCCCAGCATGTTAACGTAATAGGCGGATATCGTGTGCAGGGAAAGACCGATAAAAGTGTTAAACGATTA
TTGGAAGGCGTCAAGCTATTGGAAGAAGCGGGAGTGTTTGCCATTGTTCTCGAGCTGGTTGTTGAGGGAATAGCAAAAAA
GCTTACGGAAGAGACCAGCGTTCCTACGATAGGTATTGGTGCTGGTAGGTATTGTGACGGGCAGGTGCTTGTATGGCATG
ATCTGCTGGGAATCAATACGGAATTCTCGCCGAGATTCGTCAAGAGATACGCCAATCTGAGAAAGGACATCGTTGATGCC
CTGAAGAAGTACAATGAAGAGGTGAAGAACGGGGAATTCCCGGGACCGGAAAATGTTTTTGAGAGTGGTGGTTTTGATGA
ATAA

Upstream 100 bases:

>100_bases
AAAAAAGAAACTGAGGAATTGAAAAAACTATCACAGGATCTTTCGGAACTTTACGAACTTTTGAGCAAAACGATAATCAG
TTTCAAGGGGAGGGGAAACC

Downstream 100 bases:

>100_bases
CTGGGTGAACGTTGTTATAAAGATTGGATTGAGTCTGGCTGTTTTAGGCATATCCTTTTATATCTCAAAAACGATTTTTA
AGATTGTCTCCGCAGTTTCG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNIRQIINSKGKRKLSMVTAYSYFQAKMAEEAGIDMILVGDSYGNTILGYENTLPVTMEEMLIAVSAVRRGAPNTFVIAD
MPFLSYQVSEEKAIENAGRFIKVGANAVKLEGGAEVAGLVKKLVDFGIPVMGHLGLTPQHVNVIGGYRVQGKTDKSVKRL
LEGVKLLEEAGVFAIVLELVVEGIAKKLTEETSVPTIGIGAGRYCDGQVLVWHDLLGINTEFSPRFVKRYANLRKDIVDA
LKKYNEEVKNGEFPGPENVFESGGFDE

Sequences:

>Translated_267_residues
MNIRQIINSKGKRKLSMVTAYSYFQAKMAEEAGIDMILVGDSYGNTILGYENTLPVTMEEMLIAVSAVRRGAPNTFVIAD
MPFLSYQVSEEKAIENAGRFIKVGANAVKLEGGAEVAGLVKKLVDFGIPVMGHLGLTPQHVNVIGGYRVQGKTDKSVKRL
LEGVKLLEEAGVFAIVLELVVEGIAKKLTEETSVPTIGIGAGRYCDGQVLVWHDLLGINTEFSPRFVKRYANLRKDIVDA
LKKYNEEVKNGEFPGPENVFESGGFDE
>Mature_267_residues
MNIRQIINSKGKRKLSMVTAYSYFQAKMAEEAGIDMILVGDSYGNTILGYENTLPVTMEEMLIAVSAVRRGAPNTFVIAD
MPFLSYQVSEEKAIENAGRFIKVGANAVKLEGGAEVAGLVKKLVDFGIPVMGHLGLTPQHVNVIGGYRVQGKTDKSVKRL
LEGVKLLEEAGVFAIVLELVVEGIAKKLTEETSVPTIGIGAGRYCDGQVLVWHDLLGINTEFSPRFVKRYANLRKDIVDA
LKKYNEEVKNGEFPGPENVFESGGFDE

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=250, Percent_Identity=44.8, Blast_Score=227, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6319653, Length=248, Percent_Identity=37.9032258064516, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_KOSOT (C5CFR2)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941414.1
- GeneID:   7968125
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1724
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29174; Mature: 29174

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: ACT_SITE 178-178 BINDING 80-80 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRQIINSKGKRKLSMVTAYSYFQAKMAEEAGIDMILVGDSYGNTILGYENTLPVTMEE
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHH
MLIAVSAVRRGAPNTFVIADMPFLSYQVSEEKAIENAGRFIKVGANAVKLEGGAEVAGLV
HHHHHHHHHCCCCCEEEEECCCHHHEECCHHHHHHHCCCEEEECCCEEEECCCHHHHHHH
KKLVDFGIPVMGHLGLTPQHVNVIGGYRVQGKTDKSVKRLLEGVKLLEEAGVFAIVLELV
HHHHHCCCCHHHHCCCCCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
VEGIAKKLTEETSVPTIGIGAGRYCDGQVLVWHDLLGINTEFSPRFVKRYANLRKDIVDA
HHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHH
LKKYNEEVKNGEFPGPENVFESGGFDE
HHHHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MNIRQIINSKGKRKLSMVTAYSYFQAKMAEEAGIDMILVGDSYGNTILGYENTLPVTMEE
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHH
MLIAVSAVRRGAPNTFVIADMPFLSYQVSEEKAIENAGRFIKVGANAVKLEGGAEVAGLV
HHHHHHHHHCCCCCEEEEECCCHHHEECCHHHHHHHCCCEEEECCCEEEECCCHHHHHHH
KKLVDFGIPVMGHLGLTPQHVNVIGGYRVQGKTDKSVKRLLEGVKLLEEAGVFAIVLELV
HHHHHCCCCHHHHCCCCCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
VEGIAKKLTEETSVPTIGIGAGRYCDGQVLVWHDLLGINTEFSPRFVKRYANLRKDIVDA
HHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHH
LKKYNEEVKNGEFPGPENVFESGGFDE
HHHHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA