Click here to switch to the map view.

The map label for this gene is punA [H]

Identifier: 239618080

GI number: 239618080

Start: 1841214

End: 1842074

Strand: Direct

Name: punA [H]

Synonym: Kole_1712

Alternate gene names: 239618080

Gene position: 1841214-1842074 (Clockwise)

Preceding gene: 239618072

Following gene: 239618091

Centisome position: 79.98

GC content: 43.9

Gene sequence:

>861_bases
ATGTCTATTTTTGATCTCAAAAAAGAAATTCAGGAATATGTTCAAAAGGTAAAAAATTCAGCTCATTATCTCGAAGAGAG
ATTTCCTGAGAATCCAAAGATAGGTATTATCCTTGGGTCCGGTTTGGGTGATATATCCAAAAGTCTCGAAGATCCCATTG
CAGTGCCTTATTCTGAGATACCTGACTTTCCAGAATCAACAGCTCCCGGGCATAAAGGAGAACTGATTTTTGGTAAAATC
AGTGATCAACCGGTTATCCTTATGAACGGAAGGTTCCATTATTATGAGGGTTATGATATGCGTACCGTGACTTTTCCGAT
ACGTGTCATGCAGGAACTTGGCGTCCAGATTTTAGTGGTTACCAATGCTGCTGGTGGACTCGATCCCGATTTTGAAGTAG
GTCATCCGATGATAATAAGGGATCATATAAACTTCATGGGGAATAACCCGCTGATTGGCCCCAATATTGAAAAATGGGGG
CCAAGATTCCCGGATATGAGTGAACCCTATGACAGAAAACTCAGCGAATTAGCCGTTCTTAGTGCTAAGGAGCTTGGTAT
CGGAGTATATCAGGGTGTTTACGTTGGTGTTTCCGGGCCGAGTTTCGAAACCCCGGCAGAGCTCAGGATGTTGAGACGCT
TTGGAGCTGATGCTGTTGGAATGTCCACAGTTCCGGAGGTTATAGTAGCTAAACACGCGGGAATGCGTGTCCTGGGGATT
TCTGCCATCACAGATAGAGCCGTTCCTGACGATTTGAAACCTCTTACAGCGGAAGAGGTTATAGAGGTTGCGAACAGAAC
GGGGAAACAGATTGCAGCAATTATCCTCAAAGTTATGCAGAAGATAAAAGAATCGAAGTGA

Upstream 100 bases:

>100_bases
ACTATTTCGGTTGTGATTATAACATGTCCAGACATAGTTTTCCTGATTTGTTATAATCTAATTGAAGTTAACAAGTTGAA
TTAGTGAGGAGGGAAACAGA

Downstream 100 bases:

>100_bases
TTTCGAAGATAAAAAACGGGGAGACAGTTTGTCTCCCCATTTTTTATTTGGTGGGTGCGGCAGGGATTGAACCTACGACC
TCTTCCTCGTCAAGGAAGCG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSIFDLKKEIQEYVQKVKNSAHYLEERFPENPKIGIILGSGLGDISKSLEDPIAVPYSEIPDFPESTAPGHKGELIFGKI
SDQPVILMNGRFHYYEGYDMRTVTFPIRVMQELGVQILVVTNAAGGLDPDFEVGHPMIIRDHINFMGNNPLIGPNIEKWG
PRFPDMSEPYDRKLSELAVLSAKELGIGVYQGVYVGVSGPSFETPAELRMLRRFGADAVGMSTVPEVIVAKHAGMRVLGI
SAITDRAVPDDLKPLTAEEVIEVANRTGKQIAAIILKVMQKIKESK

Sequences:

>Translated_286_residues
MSIFDLKKEIQEYVQKVKNSAHYLEERFPENPKIGIILGSGLGDISKSLEDPIAVPYSEIPDFPESTAPGHKGELIFGKI
SDQPVILMNGRFHYYEGYDMRTVTFPIRVMQELGVQILVVTNAAGGLDPDFEVGHPMIIRDHINFMGNNPLIGPNIEKWG
PRFPDMSEPYDRKLSELAVLSAKELGIGVYQGVYVGVSGPSFETPAELRMLRRFGADAVGMSTVPEVIVAKHAGMRVLGI
SAITDRAVPDDLKPLTAEEVIEVANRTGKQIAAIILKVMQKIKESK
>Mature_285_residues
SIFDLKKEIQEYVQKVKNSAHYLEERFPENPKIGIILGSGLGDISKSLEDPIAVPYSEIPDFPESTAPGHKGELIFGKIS
DQPVILMNGRFHYYEGYDMRTVTFPIRVMQELGVQILVVTNAAGGLDPDFEVGHPMIIRDHINFMGNNPLIGPNIEKWGP
RFPDMSEPYDRKLSELAVLSAKELGIGVYQGVYVGVSGPSFETPAELRMLRRFGADAVGMSTVPEVIVAKHAGMRVLGIS
AITDRAVPDDLKPLTAEEVIEVANRTGKQIAAIILKVMQKIKESK

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=272, Percent_Identity=49.2647058823529, Blast_Score=267, Evalue=7e-72,
Organism=Homo sapiens, GI47132622, Length=260, Percent_Identity=28.0769230769231, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=39.6825396825397, Blast_Score=202, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17541190, Length=255, Percent_Identity=40.7843137254902, Blast_Score=210, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6323238, Length=266, Percent_Identity=46.9924812030075, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=44.6153846153846, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24656090, Length=260, Percent_Identity=44.6153846153846, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45552885, Length=260, Percent_Identity=44.6153846153846, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45552887, Length=260, Percent_Identity=44.6153846153846, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24762376, Length=274, Percent_Identity=35.036496350365, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20130079, Length=228, Percent_Identity=28.5087719298246, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 31560; Mature: 31429

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFDLKKEIQEYVQKVKNSAHYLEERFPENPKIGIILGSGLGDISKSLEDPIAVPYSEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHC
PDFPESTAPGHKGELIFGKISDQPVILMNGRFHYYEGYDMRTVTFPIRVMQELGVQILVV
CCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEEECCCCEEEEEHHHHHHHHCCCEEEEE
TNAAGGLDPDFEVGHPMIIRDHINFMGNNPLIGPNIEKWGPRFPDMSEPYDRKLSELAVL
ECCCCCCCCCCCCCCCEEEEECHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHH
SAKELGIGVYQGVYVGVSGPSFETPAELRMLRRFGADAVGMSTVPEVIVAKHAGMRVLGI
HHHHHCCEEECCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEE
SAITDRAVPDDLKPLTAEEVIEVANRTGKQIAAIILKVMQKIKESK
HHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIFDLKKEIQEYVQKVKNSAHYLEERFPENPKIGIILGSGLGDISKSLEDPIAVPYSEI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHC
PDFPESTAPGHKGELIFGKISDQPVILMNGRFHYYEGYDMRTVTFPIRVMQELGVQILVV
CCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEEECCCCEEEEEHHHHHHHHCCCEEEEE
TNAAGGLDPDFEVGHPMIIRDHINFMGNNPLIGPNIEKWGPRFPDMSEPYDRKLSELAVL
ECCCCCCCCCCCCCCCEEEEECHHHCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHH
SAKELGIGVYQGVYVGVSGPSFETPAELRMLRRFGADAVGMSTVPEVIVAKHAGMRVLGI
HHHHHCCEEECCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEE
SAITDRAVPDDLKPLTAEEVIEVANRTGKQIAAIILKVMQKIKESK
HHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]