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The map label for this gene is eno [H]

Identifier: 239618073

GI number: 239618073

Start: 1834723

End: 1836009

Strand: Reverse

Name: eno [H]

Synonym: Kole_1705

Alternate gene names: 239618073

Gene position: 1836009-1834723 (Counterclockwise)

Preceding gene: 239618074

Following gene: 239618070

Centisome position: 79.75

GC content: 45.45

Gene sequence:

>1287_bases
ATGTACGCCCAGATAGTTGATGTAATCGCAAGAGAGGTTCTCGATTCACGCGGAACACCAACAGTAGAAGTTGAAGTCTG
GCTGGACGATGGAGCCTATGGAAGGGCAATCGTTCCATCAGGAGCCTCTACTGGAAAGTTTGAGGCTCTCGAATTGAGAG
ATGGAGATAAAAAGAGATTTCTCGGTAAAGGTGTTTTGAAAGCTGTAGACAACGTGAACGAGGTTATCGCACCTGAAATC
ATTGGAATGAACGCTTTCGCCCAAACAGAGATCGACAGGATTCTCCTGGAACTCGATGGTACGGAATCAAAGGAAAAACT
CGGTGCCAACGCGATCCTTGGCGTATCCATGGCGGTAGCTAGAGCAGCAGCAGAAAGCCTCGGCCTTCCATTGTATCAAT
ACCTTGGCGGAGTCAACGCCAAACAGCTTCCGGTCCCTTTCATGAACATCATTAATGGTGGTAAACACGCAGACAATAAT
CTCGATATCCAGGAATTCATGATTGTCCCGGCAGGCTTCAAAACCTTCAAAGACGCTCTGAGAGCAGGTGTGGAGACATT
CCAACACCTGAAAAAATTGCTGAAAGCTGACGGACACGTCACTGCCGTTGGTGACGAAGGTGGTTTCGCTCCGAATTTCT
CCAACAATGAAGAAGCCATTCAGTACATTATAAAAGCTATTGAGAACGCTGGCTACAAAGCGGGAGAAGAGATTTTTGTT
GCTCTTGATTGTGCAGCAAGTTCATTCTACGATGAAGAGAAAAACATATACTACATCGATGGCAAGGAAATGACTTCGGA
AGATCTTATAAACTACTATGAAGACCTCGTTTCCAGATATCCGATAATCAGCATTGAAGACCCATTCTTCGAGGAAGACT
GGGAAGCCTTCACAAAGTTCAACGAAAGAGTCGGCAAAAAAGTCCAGATCGTTGGAGACGACCTGTACGTTACCAACTTG
AAAAGACTTAAGAAGGGTGTGGAAAATAACTCTTCCAACTCTATCCTGATAAAGCTCAACCAGATCGGTTCAGTTACCGA
GACTCTGGATACCATAGAGTACGCTCAGAAACACGGAATGACTTGTGTTATTTCCCACAGATCCGGTGAAACAGAAGACA
CGTTCATCGCTCATCTTGCAGTAGCGGCAAATACCGGCATGATAAAGACAGGTTCAGCCTCAAGAAGCGAAAGAATTGCC
AAGTACAACGAGCTTCTTAGAATCGAGGAAGAGCTGGGAGAAGACGCCAAGTTCCTGGGTCTGGATTCTTTCTACAACAT
AAAGTAA

Upstream 100 bases:

>100_bases
TCGTTATCGATTTCTTTACCAGCGGAAATCTGCTGCGTGCTATAATTGGCGAGAAAGTTGGTACTCTGGTAATTCCCGAC
TGAATACAGGAGGTGTTAAT

Downstream 100 bases:

>100_bases
ATTCGATTTATCAAGGGTAAAAACATGCCACGGGAATTGTTCCCGTGGTATGTTTTATTGTACCTTACCTGTTTCAGAAT
ATTCACTCAAAATTTCCAGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MYAQIVDVIAREVLDSRGTPTVEVEVWLDDGAYGRAIVPSGASTGKFEALELRDGDKKRFLGKGVLKAVDNVNEVIAPEI
IGMNAFAQTEIDRILLELDGTESKEKLGANAILGVSMAVARAAAESLGLPLYQYLGGVNAKQLPVPFMNIINGGKHADNN
LDIQEFMIVPAGFKTFKDALRAGVETFQHLKKLLKADGHVTAVGDEGGFAPNFSNNEEAIQYIIKAIENAGYKAGEEIFV
ALDCAASSFYDEEKNIYYIDGKEMTSEDLINYYEDLVSRYPIISIEDPFFEEDWEAFTKFNERVGKKVQIVGDDLYVTNL
KRLKKGVENNSSNSILIKLNQIGSVTETLDTIEYAQKHGMTCVISHRSGETEDTFIAHLAVAANTGMIKTGSASRSERIA
KYNELLRIEEELGEDAKFLGLDSFYNIK

Sequences:

>Translated_428_residues
MYAQIVDVIAREVLDSRGTPTVEVEVWLDDGAYGRAIVPSGASTGKFEALELRDGDKKRFLGKGVLKAVDNVNEVIAPEI
IGMNAFAQTEIDRILLELDGTESKEKLGANAILGVSMAVARAAAESLGLPLYQYLGGVNAKQLPVPFMNIINGGKHADNN
LDIQEFMIVPAGFKTFKDALRAGVETFQHLKKLLKADGHVTAVGDEGGFAPNFSNNEEAIQYIIKAIENAGYKAGEEIFV
ALDCAASSFYDEEKNIYYIDGKEMTSEDLINYYEDLVSRYPIISIEDPFFEEDWEAFTKFNERVGKKVQIVGDDLYVTNL
KRLKKGVENNSSNSILIKLNQIGSVTETLDTIEYAQKHGMTCVISHRSGETEDTFIAHLAVAANTGMIKTGSASRSERIA
KYNELLRIEEELGEDAKFLGLDSFYNIK
>Mature_428_residues
MYAQIVDVIAREVLDSRGTPTVEVEVWLDDGAYGRAIVPSGASTGKFEALELRDGDKKRFLGKGVLKAVDNVNEVIAPEI
IGMNAFAQTEIDRILLELDGTESKEKLGANAILGVSMAVARAAAESLGLPLYQYLGGVNAKQLPVPFMNIINGGKHADNN
LDIQEFMIVPAGFKTFKDALRAGVETFQHLKKLLKADGHVTAVGDEGGFAPNFSNNEEAIQYIIKAIENAGYKAGEEIFV
ALDCAASSFYDEEKNIYYIDGKEMTSEDLINYYEDLVSRYPIISIEDPFFEEDWEAFTKFNERVGKKVQIVGDDLYVTNL
KRLKKGVENNSSNSILIKLNQIGSVTETLDTIEYAQKHGMTCVISHRSGETEDTFIAHLAVAANTGMIKTGSASRSERIA
KYNELLRIEEELGEDAKFLGLDSFYNIK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=53.4883720930233, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=50.5747126436782, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=50.5747126436782, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50.4651162790698, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=46.6512702078522, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=26.6862170087977, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=26.6862170087977, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=26.6862170087977, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=57.6832151300236, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=50.8158508158508, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=50.8120649651972, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.3541666666667, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=50.3464203233256, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=48.8479262672811, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=48.6175115207373, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=48.6175115207373, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.4226327944573, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=426, Percent_Identity=49.5305164319249, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=426, Percent_Identity=49.5305164319249, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=426, Percent_Identity=49.5305164319249, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=426, Percent_Identity=49.5305164319249, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=426, Percent_Identity=49.5305164319249, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=426, Percent_Identity=49.5305164319249, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47171; Mature: 47171

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAQIVDVIAREVLDSRGTPTVEVEVWLDDGAYGRAIVPSGASTGKFEALELRDGDKKRF
CHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCCCCCEEEEEECCCCHHHH
LGKGVLKAVDNVNEVIAPEIIGMNAFAQTEIDRILLELDGTESKEKLGANAILGVSMAVA
HHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHEEEEECCCCCHHHHCCCHHHHHHHHHH
RAAAESLGLPLYQYLGGVNAKQLPVPFMNIINGGKHADNNLDIQEFMIVPAGFKTFKDAL
HHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHH
RAGVETFQHLKKLLKADGHVTAVGDEGGFAPNFSNNEEAIQYIIKAIENAGYKAGEEIFV
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
ALDCAASSFYDEEKNIYYIDGKEMTSEDLINYYEDLVSRYPIISIEDPFFEEDWEAFTKF
EEEEHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
NERVGKKVQIVGDDLYVTNLKRLKKGVENNSSNSILIKLNQIGSVTETLDTIEYAQKHGM
HHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
TCVISHRSGETEDTFIAHLAVAANTGMIKTGSASRSERIAKYNELLRIEEELGEDAKFLG
EEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
LDSFYNIK
CCCCCCCC
>Mature Secondary Structure
MYAQIVDVIAREVLDSRGTPTVEVEVWLDDGAYGRAIVPSGASTGKFEALELRDGDKKRF
CHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCCCCCEEEEEECCCCHHHH
LGKGVLKAVDNVNEVIAPEIIGMNAFAQTEIDRILLELDGTESKEKLGANAILGVSMAVA
HHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHEEEEECCCCCHHHHCCCHHHHHHHHHH
RAAAESLGLPLYQYLGGVNAKQLPVPFMNIINGGKHADNNLDIQEFMIVPAGFKTFKDAL
HHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHH
RAGVETFQHLKKLLKADGHVTAVGDEGGFAPNFSNNEEAIQYIIKAIENAGYKAGEEIFV
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
ALDCAASSFYDEEKNIYYIDGKEMTSEDLINYYEDLVSRYPIISIEDPFFEEDWEAFTKF
EEEEHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
NERVGKKVQIVGDDLYVTNLKRLKKGVENNSSNSILIKLNQIGSVTETLDTIEYAQKHGM
HHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
TCVISHRSGETEDTFIAHLAVAANTGMIKTGSASRSERIAKYNELLRIEEELGEDAKFLG
EEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
LDSFYNIK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA