| Definition | Kosmotoga olearia TBF 19.5.1, complete genome. |
|---|---|
| Accession | NC_012785 |
| Length | 2,302,126 |
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The map label for this gene is pulA [H]
Identifier: 239618034
GI number: 239618034
Start: 1791687
End: 1794269
Strand: Direct
Name: pulA [H]
Synonym: Kole_1663
Alternate gene names: 239618034
Gene position: 1791687-1794269 (Clockwise)
Preceding gene: 239618033
Following gene: 239618035
Centisome position: 77.83
GC content: 42.78
Gene sequence:
>2583_bases ATGAAGTTTCTGAGAGTATTTTTTCTGGTGTTAATATTCATGGTGTTTGTGGAGACGGTTTTTGGGTTTGAAGCGTTGGC AACCGGTAAATCCGCGAAGGATTTTGGGGGTAATACGGTTTTGATCGTTCATTATCATCGTTACGATGGAAACTATAATG GTTGGAATCTTTGGGTATGGCCTCATAAACCGCAAAGTCTTGAGGGGAAGAGCTATAAGTTCACAGGTAAAGATGATTTT GGAGTACTGTCTATTGTTGTTTTCCAGGACAGATACACGCAATTAGGTTTCATCGTAAGGTTGAGGGAATGGGAAGCAAA GGATATTAACGCCGACCGATTTGTCGATATTCCGGAATCAGGTGTTGCCGAAATATGGGTTTTGGAAGGAGAAATGGAGT ATTACACAGATCCTTCTAACGTTGATCTTAGTCCGAGGGTGAAGGCGGCATTTTTGGATAGTTTTCAGGAAATTTATGCC TACCTCTCAACTCCTTTTAACACCAACAAATGGAAAGGAAAACTTTTGATCAGTATTGATGGAAAAAGCGTGGCGGTTAA AGATGTAACAAAAGCAGATCCAACGGACATTTCTACTACAAAATATATAAAGATAATCCTTGAATCGCCCTTAAAACCAA AGGATGTTTCAAAAGATATAAAGTTGAATATAAGAGGTTACATTGAAAAAACTGTTATTGTACGGAAAGTCCTTGACGAT GATGAATTTTATTACAGTGGGGATGACCTTGGAGCAAACTATTCACCTCAGCGTACCGTTTTCAAAATCTGGTCACCGGT TTCTTCGAAAGTGGAACTACTCCTTTACGATGATTTCAATTCGGAAAAACCGTCAAAGGTTGTGGAAATGCAAAGAGCCT CTGAAGGGGTATGGACAGCTACTGTTGAGGGAGATCTTCACCTTACAGCATATAAATATCGCTTCTATTCCTACGGTAAG ATCAGGGAAACCGTGGACATTCATTCCAGGGCGGTTACCAGAAACAGTGAAAAGTCTGTAATTGTTGATCTATCCAAGAC GAATTTTTTCGGTTGGGAGAAGGAGGTAAAACCAATCCTGCTCGCTCCGGAGGATTCTATAATTTATGAAATTCACATCG CCGATATCACCGCTGATCAATCAACGAATATCGTTCACAAGGGGAAATATCTCGGACTTACCGAAAAAGGCGCAACGGGC CCCGAAGGAGTAAAAGTGGGGCTTGACCATCTTGTGGAATTGGGAATTACCCATGTCCATATAATGCCGATTCATGATAT TTATTACATCAAAGAAGGCGAGCCGGATCAGTATGGCTGGGGCTATGATCCCTATCTTTACTGGGTTCCCGAAGGGCATT ACAGTACGAATCCTTCCGATCCTCTTTCAAGAATCGTTGAGGTAAAAAAGATGATAAAGGCCCTTCATGAAAATAATATC AGGGTTATCCTTGATGTGGTTTATAACCATACTGCTCAAACCGGCCCGGGCTCTCCTTTCGACCAGACGGTTCCTTATTA CTTCTATCGAACAGATAAAGCCGGGAATTATACGAATGGCTCAGGTGTTGGAAACGAAATTGCCACCGAACGACCGATGA TGAGAAAGTACATTGTTGATTCACTGCTTTACTGGGTTAGGGAGTATCATGTTGATGGTTTCAGGTTTGATCTTCTTGGC CTGTTTGACAGGAGAACAGTTTTAGAGATAGAAAAGGCTCTCCATAAGGTGGATCCAACGATACTTCTGTATGGTGAACC CTGGGGAGGCTTTGGTGCGAAGGTTACCTTTACCAAAGGCGATCAAAGAGGGACCAATGTTGCCCTTTTTAACGATGGTT TCAGAGATGCCATCCGGGGTAGCGTTTTCAATAAGACGGCGAAAGGCTTCGCGTTTGGTTCAAGAGGGAAGGAAACAAGA ATTAAACGCGGTGTTGTGGGCAGTATAAAATACGACAGCAAAATCGTTGATTTTGCCGCGGATCCTGAAGAAACGATAAA CTACGTTTCTTCCCACGACAATCAAACCCTCTGGGATAAGAACCTGAGCGCTATGTCGAGTGCTTCCGAAGAGTTTCTTA AGTCAGCCCAGAAATTATCGAATGCGATCATTTTGCTTTCTCAGGGAATCCCATTCCTTCACGGCGGTAGCGACTTCTGC CGGACCAAGTATGGTAACGAAAACAGTTATAATGCCGGAATTGAGATCAACAAATTCGATTGGGCGAGAAAAGTCAAATT TCTGGATGTTTTCGAATATTACAAAGGGCTTATAGAGCTCAGGAAAAAGCACATCGCCTTCAGGATGAGGACGGCAGAGG ATATACGCTCGAACCTTACCTTTATAAACACGCCGCAAAAGCGCATGGTAGCGTTTATTATAGACGGCAGAAAGCTTGGA GACACCTGGAACAAAATTCTTGTGGTTTTCAACGCCAACACTTCCCCCGTTTATTTCAGGTTGCCGGATGGGGAATGGGA AATGGTTGTTGATGGGAAGATTGCCGGGATAGCCACAATAAAAACCGTTAAGGGTATCGTAAGGCTTGATGGAACAAGTG CGTATGTGTTTCATAGTAAATAA
Upstream 100 bases:
>100_bases CAGTTTATTTGTGGCAGTACATAACAAGAGTATGAAGAAGGGAGGTACGGGTGTTCTTTCTTCCCCGGTTAAACGGGAAG CGTTTGGATGCCGAAAGCCT
Downstream 100 bases:
>100_bases CATAAAATGATGGGCATTTTGGAGGTGCACCATGTACAGAAGCTCTGTAATAAGGGTTCTTGAAACCGGCTATCTGGATG AAACCCGTTCCGCCGCACTG
Product: pullulanase, type I
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 860; Mature: 860
Protein sequence:
>860_residues MKFLRVFFLVLIFMVFVETVFGFEALATGKSAKDFGGNTVLIVHYHRYDGNYNGWNLWVWPHKPQSLEGKSYKFTGKDDF GVLSIVVFQDRYTQLGFIVRLREWEAKDINADRFVDIPESGVAEIWVLEGEMEYYTDPSNVDLSPRVKAAFLDSFQEIYA YLSTPFNTNKWKGKLLISIDGKSVAVKDVTKADPTDISTTKYIKIILESPLKPKDVSKDIKLNIRGYIEKTVIVRKVLDD DEFYYSGDDLGANYSPQRTVFKIWSPVSSKVELLLYDDFNSEKPSKVVEMQRASEGVWTATVEGDLHLTAYKYRFYSYGK IRETVDIHSRAVTRNSEKSVIVDLSKTNFFGWEKEVKPILLAPEDSIIYEIHIADITADQSTNIVHKGKYLGLTEKGATG PEGVKVGLDHLVELGITHVHIMPIHDIYYIKEGEPDQYGWGYDPYLYWVPEGHYSTNPSDPLSRIVEVKKMIKALHENNI RVILDVVYNHTAQTGPGSPFDQTVPYYFYRTDKAGNYTNGSGVGNEIATERPMMRKYIVDSLLYWVREYHVDGFRFDLLG LFDRRTVLEIEKALHKVDPTILLYGEPWGGFGAKVTFTKGDQRGTNVALFNDGFRDAIRGSVFNKTAKGFAFGSRGKETR IKRGVVGSIKYDSKIVDFAADPEETINYVSSHDNQTLWDKNLSAMSSASEEFLKSAQKLSNAIILLSQGIPFLHGGSDFC RTKYGNENSYNAGIEINKFDWARKVKFLDVFEYYKGLIELRKKHIAFRMRTAEDIRSNLTFINTPQKRMVAFIIDGRKLG DTWNKILVVFNANTSPVYFRLPDGEWEMVVDGKIAGIATIKTVKGIVRLDGTSAYVFHSK
Sequences:
>Translated_860_residues MKFLRVFFLVLIFMVFVETVFGFEALATGKSAKDFGGNTVLIVHYHRYDGNYNGWNLWVWPHKPQSLEGKSYKFTGKDDF GVLSIVVFQDRYTQLGFIVRLREWEAKDINADRFVDIPESGVAEIWVLEGEMEYYTDPSNVDLSPRVKAAFLDSFQEIYA YLSTPFNTNKWKGKLLISIDGKSVAVKDVTKADPTDISTTKYIKIILESPLKPKDVSKDIKLNIRGYIEKTVIVRKVLDD DEFYYSGDDLGANYSPQRTVFKIWSPVSSKVELLLYDDFNSEKPSKVVEMQRASEGVWTATVEGDLHLTAYKYRFYSYGK IRETVDIHSRAVTRNSEKSVIVDLSKTNFFGWEKEVKPILLAPEDSIIYEIHIADITADQSTNIVHKGKYLGLTEKGATG PEGVKVGLDHLVELGITHVHIMPIHDIYYIKEGEPDQYGWGYDPYLYWVPEGHYSTNPSDPLSRIVEVKKMIKALHENNI RVILDVVYNHTAQTGPGSPFDQTVPYYFYRTDKAGNYTNGSGVGNEIATERPMMRKYIVDSLLYWVREYHVDGFRFDLLG LFDRRTVLEIEKALHKVDPTILLYGEPWGGFGAKVTFTKGDQRGTNVALFNDGFRDAIRGSVFNKTAKGFAFGSRGKETR IKRGVVGSIKYDSKIVDFAADPEETINYVSSHDNQTLWDKNLSAMSSASEEFLKSAQKLSNAIILLSQGIPFLHGGSDFC RTKYGNENSYNAGIEINKFDWARKVKFLDVFEYYKGLIELRKKHIAFRMRTAEDIRSNLTFINTPQKRMVAFIIDGRKLG DTWNKILVVFNANTSPVYFRLPDGEWEMVVDGKIAGIATIKTVKGIVRLDGTSAYVFHSK >Mature_860_residues MKFLRVFFLVLIFMVFVETVFGFEALATGKSAKDFGGNTVLIVHYHRYDGNYNGWNLWVWPHKPQSLEGKSYKFTGKDDF GVLSIVVFQDRYTQLGFIVRLREWEAKDINADRFVDIPESGVAEIWVLEGEMEYYTDPSNVDLSPRVKAAFLDSFQEIYA YLSTPFNTNKWKGKLLISIDGKSVAVKDVTKADPTDISTTKYIKIILESPLKPKDVSKDIKLNIRGYIEKTVIVRKVLDD DEFYYSGDDLGANYSPQRTVFKIWSPVSSKVELLLYDDFNSEKPSKVVEMQRASEGVWTATVEGDLHLTAYKYRFYSYGK IRETVDIHSRAVTRNSEKSVIVDLSKTNFFGWEKEVKPILLAPEDSIIYEIHIADITADQSTNIVHKGKYLGLTEKGATG PEGVKVGLDHLVELGITHVHIMPIHDIYYIKEGEPDQYGWGYDPYLYWVPEGHYSTNPSDPLSRIVEVKKMIKALHENNI RVILDVVYNHTAQTGPGSPFDQTVPYYFYRTDKAGNYTNGSGVGNEIATERPMMRKYIVDSLLYWVREYHVDGFRFDLLG LFDRRTVLEIEKALHKVDPTILLYGEPWGGFGAKVTFTKGDQRGTNVALFNDGFRDAIRGSVFNKTAKGFAFGSRGKETR IKRGVVGSIKYDSKIVDFAADPEETINYVSSHDNQTLWDKNLSAMSSASEEFLKSAQKLSNAIILLSQGIPFLHGGSDFC RTKYGNENSYNAGIEINKFDWARKVKFLDVFEYYKGLIELRKKHIAFRMRTAEDIRSNLTFINTPQKRMVAFIIDGRKLG DTWNKILVVFNANTSPVYFRLPDGEWEMVVDGKIAGIATIKTVKGIVRLDGTSAYVFHSK
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=667, Percent_Identity=25.6371814092954, Blast_Score=136, Evalue=6e-33,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 97944; Mature: 97944
Theoretical pI: Translated: 7.63; Mature: 7.63
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFLRVFFLVLIFMVFVETVFGFEALATGKSAKDFGGNTVLIVHYHRYDGNYNGWNLWVW CHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHCCCCEEEEEEEEEECCCCCCEEEEEE PHKPQSLEGKSYKFTGKDDFGVLSIVVFQDRYTQLGFIVRLREWEAKDINADRFVDIPES CCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEECCCC GVAEIWVLEGEMEYYTDPSNVDLSPRVKAAFLDSFQEIYAYLSTPFNTNKWKGKLLISID CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC GKSVAVKDVTKADPTDISTTKYIKIILESPLKPKDVSKDIKLNIRGYIEKTVIVRKVLDD CCEEEEEECCCCCCCCCCCHHEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCC DEFYYSGDDLGANYSPQRTVFKIWSPVSSKVELLLYDDFNSEKPSKVVEMQRASEGVWTA CCEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEE TVEGDLHLTAYKYRFYSYGKIRETVDIHSRAVTRNSEKSVIVDLSKTNFFGWEKEVKPIL EECCCEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEE LAPEDSIIYEIHIADITADQSTNIVHKGKYLGLTEKGATGPEGVKVGLDHLVELGITHVH ECCCCCEEEEEEEEEEECCCCCCEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEE IMPIHDIYYIKEGEPDQYGWGYDPYLYWVPEGHYSTNPSDPLSRIVEVKKMIKALHENNI EEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC RVILDVVYNHTAQTGPGSPFDQTVPYYFYRTDKAGNYTNGSGVGNEIATERPMMRKYIVD EEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHHH SLLYWVREYHVDGFRFDLLGLFDRRTVLEIEKALHKVDPTILLYGEPWGGFGAKVTFTKG HHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECC DQRGTNVALFNDGFRDAIRGSVFNKTAKGFAFGSRGKETRIKRGVVGSIKYDSKIVDFAA CCCCCEEEEECCCCHHHHCCHHHCCCCCCCEECCCCCCHHHHCCCEEEEEECCEEEEECC DPEETINYVSSHDNQTLWDKNLSAMSSASEEFLKSAQKLSNAIILLSQGIPFLHGGSDFC CHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCHHH RTKYGNENSYNAGIEINKFDWARKVKFLDVFEYYKGLIELRKKHIAFRMRTAEDIRSNLT HCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCE FINTPQKRMVAFIIDGRKLGDTWNKILVVFNANTSPVYFRLPDGEWEMVVDGKIAGIATI EEECCHHHEEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEH KTVKGIVRLDGTSAYVFHSK HHHCEEEEECCCEEEEEECC >Mature Secondary Structure MKFLRVFFLVLIFMVFVETVFGFEALATGKSAKDFGGNTVLIVHYHRYDGNYNGWNLWVW CHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHCCCCEEEEEEEEEECCCCCCEEEEEE PHKPQSLEGKSYKFTGKDDFGVLSIVVFQDRYTQLGFIVRLREWEAKDINADRFVDIPES CCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEECCCC GVAEIWVLEGEMEYYTDPSNVDLSPRVKAAFLDSFQEIYAYLSTPFNTNKWKGKLLISID CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC GKSVAVKDVTKADPTDISTTKYIKIILESPLKPKDVSKDIKLNIRGYIEKTVIVRKVLDD CCEEEEEECCCCCCCCCCCHHEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCC DEFYYSGDDLGANYSPQRTVFKIWSPVSSKVELLLYDDFNSEKPSKVVEMQRASEGVWTA CCEEECCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEE TVEGDLHLTAYKYRFYSYGKIRETVDIHSRAVTRNSEKSVIVDLSKTNFFGWEKEVKPIL EECCCEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEE LAPEDSIIYEIHIADITADQSTNIVHKGKYLGLTEKGATGPEGVKVGLDHLVELGITHVH ECCCCCEEEEEEEEEEECCCCCCEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEE IMPIHDIYYIKEGEPDQYGWGYDPYLYWVPEGHYSTNPSDPLSRIVEVKKMIKALHENNI EEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC RVILDVVYNHTAQTGPGSPFDQTVPYYFYRTDKAGNYTNGSGVGNEIATERPMMRKYIVD EEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHHH SLLYWVREYHVDGFRFDLLGLFDRRTVLEIEKALHKVDPTILLYGEPWGGFGAKVTFTKG HHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECC DQRGTNVALFNDGFRDAIRGSVFNKTAKGFAFGSRGKETRIKRGVVGSIKYDSKIVDFAA CCCCCEEEEECCCCHHHHCCHHHCCCCCCCEECCCCCCHHHHCCCEEEEEECCEEEEECC DPEETINYVSSHDNQTLWDKNLSAMSSASEEFLKSAQKLSNAIILLSQGIPFLHGGSDFC CHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCHHH RTKYGNENSYNAGIEINKFDWARKVKFLDVFEYYKGLIELRKKHIAFRMRTAEDIRSNLT HCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCE FINTPQKRMVAFIIDGRKLGDTWNKILVVFNANTSPVYFRLPDGEWEMVVDGKIAGIATI EEECCHHHEEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEH KTVKGIVRLDGTSAYVFHSK HHHCEEEEECCCEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]