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The map label for this gene is nadE2 [H]

Identifier: 239617986

GI number: 239617986

Start: 1731080

End: 1732816

Strand: Direct

Name: nadE2 [H]

Synonym: Kole_1614

Alternate gene names: 239617986

Gene position: 1731080-1732816 (Clockwise)

Preceding gene: 239617985

Following gene: 239617987

Centisome position: 75.19

GC content: 42.72

Gene sequence:

>1737_bases
ATGATAGTTAGACTTGGACTCGCACAGATCAACACCACAGTTGGGGATTTGAAAGGTAATGTAACGAAGATAAATGAATA
CATAGATCTTGCAGAGAAAAAAGGAGTTAGCATTCTTGTTTTTCCGGAATTGGCGATTACTGGTTATCCCCCAGAGGATC
TCGTTCTGCGCACGGGGTTCATAAAAGATAATCTTTCAGCTCTTGAAAAGGTCAGAGAATATACAATAGATAAGGAACTT
CTTGTGATTTTAGGATTCGTTGATTTTGACTTTGAGATATACAACGCCGCTGCGGTTCTTCATAACGGAGAAAAAGTCGC
TGTTTATCGCAAAATGCATCTCCCAAATTATTCAGTTTTTGACGAAAAGCGATATTTTTCGGCCGGAAAAAAGGCTTTAA
TTCTGAGAAACAAAAACCTCCGCATTGGCGTGAACATCTGTGAGGATCTCTGGGTCCCTTCAGGGCCAATAAATGAACAA
ACGATATTCGGGGCAAACCTCATAATAAACCTTTCCGCTTCTCCGTTCACTGCCGGAAAGTCAAGAAAACGTTTGGAATT
ATTATCTACACGCGCTTCAGAATACTCCTGTGCAATCGCTTATTGCAACCTCGTTGGAGGCCAGGACGAGCTTGTTTTCG
ATGGATTGAGCGCGGTTGTTCTGCCAGACGGAAGGAGCTTCATAGCACGAGGGTTTGAGGAGGAACTGTTCATTACGGAT
CTGGACCTCGATATCGCAACTCGATACAATCTTTATGAAGGAAAGCGGAAAAGCTTCAAAGCGAATGCTGAACTGGAAGA
AAAGGAAATAAGCTTCACACTTCGTGATAACATCGAGTTTTTGACTTCTCACAGGATAGCGGAAGGCATAGAGGAAATTT
TCAATGCGCTTGTCCTTGGTTTGAGAGACTACGTAAAGAAAAATGGTTTTAGTACCGTGACCCTCGGTCTCAGTGGTGGT
ATGGATTCTTCTCTTGCAGCTTGTATAGCCGTTGAGGCACTCGGAAGGGAAAACGTTGTTGGTGTGTTGATGCCTTCAGA
GTACACCTCCAACACGAGCATTGAAGATGCCAAAATGCTTGCAAAGAATCTCGGGATAAAAACCCTGACCGTTCCTATAA
CTGCGATTTATCATTCATATCTTGGAACGCTTTCTGATGCTTTTAAAGGAAGAGAACAGGATGTAACGGAAGAAAATATA
CAGGCACGAATAAGAGGCAACATCCTTATGGCACTTTCTAACAAATTCGGCTGGCTTGTTCTGGCAACCGGCAACAAGAG
CGAACTGGCTACGGGGTATGCCACCCTTTATGGCGATATGGCTGGAGGCTTTGCCGTCCTTAAAGACGTATACAAAACGG
ACGTTTATAGATTGGCAGATTATTTCAACCGTACTAAGAAAACCGAGATAATCCCCAGAAGAGTTTTTGAAAAACCGCCT
TCGGCTGAACTCCGGCCAAACCAGACAGATCAGGACACACTTCCTCCATACGAAACACTGGATAAAATCCTGAAACTTTT
TATAGAGGAAGGGCTTTCAGCCAATGAAATACTCGAACATGGTTTCGATAATAATACTGTGAACTTCACGCTAAAGCTGC
TTAAGAAAAATGAATATAAGCGAAGGCAGGGGGCACCTGGAATAAAAATTTCAAGGAGGGCCTTTGGAAAAGATTGGCGA
ATGCCGATAACTAACGATTATAATACAACATTTATGGATGGTGAAAACGGTGATTAA

Upstream 100 bases:

>100_bases
GCCTGGGAAAGAAATCGAGAAATCATTGTTATAATTGAATTGAAAGAGATTGTAGACAGGCTTATAAATCATTTTAACTA
CATCTGGGAGGTTTGTTCAA

Downstream 100 bases:

>100_bases
TGGTGTCGTTATCGATATAGATGGAACTTTTATAGACAGCGAAGAGAAGATCCCGATAGAAAACCTTGAAGTTTTCAGAG
AACTTGAGGCTAAAGGCTTA

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MIVRLGLAQINTTVGDLKGNVTKINEYIDLAEKKGVSILVFPELAITGYPPEDLVLRTGFIKDNLSALEKVREYTIDKEL
LVILGFVDFDFEIYNAAAVLHNGEKVAVYRKMHLPNYSVFDEKRYFSAGKKALILRNKNLRIGVNICEDLWVPSGPINEQ
TIFGANLIINLSASPFTAGKSRKRLELLSTRASEYSCAIAYCNLVGGQDELVFDGLSAVVLPDGRSFIARGFEEELFITD
LDLDIATRYNLYEGKRKSFKANAELEEKEISFTLRDNIEFLTSHRIAEGIEEIFNALVLGLRDYVKKNGFSTVTLGLSGG
MDSSLAACIAVEALGRENVVGVLMPSEYTSNTSIEDAKMLAKNLGIKTLTVPITAIYHSYLGTLSDAFKGREQDVTEENI
QARIRGNILMALSNKFGWLVLATGNKSELATGYATLYGDMAGGFAVLKDVYKTDVYRLADYFNRTKKTEIIPRRVFEKPP
SAELRPNQTDQDTLPPYETLDKILKLFIEEGLSANEILEHGFDNNTVNFTLKLLKKNEYKRRQGAPGIKISRRAFGKDWR
MPITNDYNTTFMDGENGD

Sequences:

>Translated_578_residues
MIVRLGLAQINTTVGDLKGNVTKINEYIDLAEKKGVSILVFPELAITGYPPEDLVLRTGFIKDNLSALEKVREYTIDKEL
LVILGFVDFDFEIYNAAAVLHNGEKVAVYRKMHLPNYSVFDEKRYFSAGKKALILRNKNLRIGVNICEDLWVPSGPINEQ
TIFGANLIINLSASPFTAGKSRKRLELLSTRASEYSCAIAYCNLVGGQDELVFDGLSAVVLPDGRSFIARGFEEELFITD
LDLDIATRYNLYEGKRKSFKANAELEEKEISFTLRDNIEFLTSHRIAEGIEEIFNALVLGLRDYVKKNGFSTVTLGLSGG
MDSSLAACIAVEALGRENVVGVLMPSEYTSNTSIEDAKMLAKNLGIKTLTVPITAIYHSYLGTLSDAFKGREQDVTEENI
QARIRGNILMALSNKFGWLVLATGNKSELATGYATLYGDMAGGFAVLKDVYKTDVYRLADYFNRTKKTEIIPRRVFEKPP
SAELRPNQTDQDTLPPYETLDKILKLFIEEGLSANEILEHGFDNNTVNFTLKLLKKNEYKRRQGAPGIKISRRAFGKDWR
MPITNDYNTTFMDGENGD
>Mature_578_residues
MIVRLGLAQINTTVGDLKGNVTKINEYIDLAEKKGVSILVFPELAITGYPPEDLVLRTGFIKDNLSALEKVREYTIDKEL
LVILGFVDFDFEIYNAAAVLHNGEKVAVYRKMHLPNYSVFDEKRYFSAGKKALILRNKNLRIGVNICEDLWVPSGPINEQ
TIFGANLIINLSASPFTAGKSRKRLELLSTRASEYSCAIAYCNLVGGQDELVFDGLSAVVLPDGRSFIARGFEEELFITD
LDLDIATRYNLYEGKRKSFKANAELEEKEISFTLRDNIEFLTSHRIAEGIEEIFNALVLGLRDYVKKNGFSTVTLGLSGG
MDSSLAACIAVEALGRENVVGVLMPSEYTSNTSIEDAKMLAKNLGIKTLTVPITAIYHSYLGTLSDAFKGREQDVTEENI
QARIRGNILMALSNKFGWLVLATGNKSELATGYATLYGDMAGGFAVLKDVYKTDVYRLADYFNRTKKTEIIPRRVFEKPP
SAELRPNQTDQDTLPPYETLDKILKLFIEEGLSANEILEHGFDNNTVNFTLKLLKKNEYKRRQGAPGIKISRRAFGKDWR
MPITNDYNTTFMDGENGD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI24641841, Length=578, Percent_Identity=24.0484429065744, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24641843, Length=578, Percent_Identity=24.0484429065744, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 64599; Mature: 64599

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVRLGLAQINTTVGDLKGNVTKINEYIDLAEKKGVSILVFPELAITGYPPEDLVLRTGF
CEEEEEHHHHHCHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHEEEECC
IKDNLSALEKVREYTIDKELLVILGFVDFDFEIYNAAAVLHNGEKVAVYRKMHLPNYSVF
CHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEEEEEEEEECCCEEEEEEEECCCCCCCC
DEKRYFSAGKKALILRNKNLRIGVNICEDLWVPSGPINEQTIFGANLIINLSASPFTAGK
CCHHHHHCCCEEEEEECCCEEEEEEEHHHCCCCCCCCCCCEEEECEEEEEECCCCCCCCC
SRKRLELLSTRASEYSCAIAYCNLVGGQDELVFDGLSAVVLPDGRSFIARGFEEELFITD
CHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCEEEECCCHHHHHCCCCCCEEEEE
LDLDIATRYNLYEGKRKSFKANAELEEKEISFTLRDNIEFLTSHRIAEGIEEIFNALVLG
CCCCCEEECCCCCCCCCCEECCCCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
LRDYVKKNGFSTVTLGLSGGMDSSLAACIAVEALGRENVVGVLMPSEYTSNTSIEDAKML
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHH
AKNLGIKTLTVPITAIYHSYLGTLSDAFKGREQDVTEENIQARIRGNILMALSNKFGWLV
HHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCEEEEEECCCCEEE
LATGNKSELATGYATLYGDMAGGFAVLKDVYKTDVYRLADYFNRTKKTEIIPRRVFEKPP
EEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
SAELRPNQTDQDTLPPYETLDKILKLFIEEGLSANEILEHGFDNNTVNFTLKLLKKNEYK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCHHH
RRQGAPGIKISRRAFGKDWRMPITNDYNTTFMDGENGD
HHCCCCCCEEEHHHCCCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MIVRLGLAQINTTVGDLKGNVTKINEYIDLAEKKGVSILVFPELAITGYPPEDLVLRTGF
CEEEEEHHHHHCHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHEEEECC
IKDNLSALEKVREYTIDKELLVILGFVDFDFEIYNAAAVLHNGEKVAVYRKMHLPNYSVF
CHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEEEEEEEEECCCEEEEEEEECCCCCCCC
DEKRYFSAGKKALILRNKNLRIGVNICEDLWVPSGPINEQTIFGANLIINLSASPFTAGK
CCHHHHHCCCEEEEEECCCEEEEEEEHHHCCCCCCCCCCCEEEECEEEEEECCCCCCCCC
SRKRLELLSTRASEYSCAIAYCNLVGGQDELVFDGLSAVVLPDGRSFIARGFEEELFITD
CHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCCCEEEECCCHHHHHCCCCCCEEEEE
LDLDIATRYNLYEGKRKSFKANAELEEKEISFTLRDNIEFLTSHRIAEGIEEIFNALVLG
CCCCCEEECCCCCCCCCCEECCCCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
LRDYVKKNGFSTVTLGLSGGMDSSLAACIAVEALGRENVVGVLMPSEYTSNTSIEDAKML
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHH
AKNLGIKTLTVPITAIYHSYLGTLSDAFKGREQDVTEENIQARIRGNILMALSNKFGWLV
HHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEECCCEEEEEECCCCEEE
LATGNKSELATGYATLYGDMAGGFAVLKDVYKTDVYRLADYFNRTKKTEIIPRRVFEKPP
EEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
SAELRPNQTDQDTLPPYETLDKILKLFIEEGLSANEILEHGFDNNTVNFTLKLLKKNEYK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCHHH
RRQGAPGIKISRRAFGKDWRMPITNDYNTTFMDGENGD
HHCCCCCCEEEHHHCCCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]