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The map label for this gene is nplT [H]

Identifier: 239617948

GI number: 239617948

Start: 1691085

End: 1692359

Strand: Direct

Name: nplT [H]

Synonym: Kole_1576

Alternate gene names: 239617948

Gene position: 1691085-1692359 (Clockwise)

Preceding gene: 239617947

Following gene: 239617949

Centisome position: 73.46

GC content: 41.96

Gene sequence:

>1275_bases
ATGGCAAAGGATACGCAGGTTACGGCCTTTGACTTAGCGATTTATGAAATCTTTCCAAGAATGTTTGTGGACAAAGGGGG
AGGCGCCTTTAAGAATATCTCTGGAATTCTGTCTGAACTCAAATCATTGGGAATTAATACGATCTGGTTGATGCCTATTC
ATCCTGTTGGGATAGAGAATCGCAAAGGTGCTTCCGGGTCTCCGTATGCGGTAAGAGATTATTTTGAGATCGATTCAAAA
CTCGGAAGCAAAGAAGATTACCGTGTTTTTGTAAAAACAGCGCATTCGTATGGTATAAAGGTTTTTATGGACATGGTGAT
GAATCACTGCGCGTGTGATTCTGTTTTAGTGAGGGAACACCCGGATTGGATCTTGAAAGATGGTCAGGGAAAATTCGGTC
GCAAGATAGCCGAATGGGAGGATGTTTATGACCTTGACTATCACAACCAGCAACTGGTTGATTACATGGTAAGCGTTTTG
AGGTACTGGGTCGAAGAATTTGACATTGACGGTTATCGTTGTGATGTGGCCGATCTGGTTCCTCTCAGTTTTTGGTTGAA
GGCAAGGCATGAAATCTCCATGATAAAGCCCGATTTTTTATGGCTTTCAGAAGGCTACGAAAATGAAATGTACCGGGCTT
TCGATATCACATATGATTACGATGGTTTTACAAACTTTCTTCAATATCTCGAGAAAAAGATCCCATTGATGGATTATATT
TCCTATTTGAATTATCAGCATAGGAATTCGCCCAAAAATGCTGTAAAGTTACGTTTCCTGGAGAACCATGACCAACTAAG
GATTTCAGGCAGGCTGGGAGTCGAAGAAGTCTTGGAATGGACGGCGTTTATGCTCATGCTTCGTGGGGTTCCCCTGATAT
ATAATGGTCAGGAATATCTCAAGGGTTCCCGACCGGATATTTTTGATTATCCTCCATTGTATGAAAAAAAGAACCTTTGG
TTCAGGGATTTTCTGGCCGGGTTGCTGAAGCTCAGAAGTAAATCACCTGCGTTGAGGTACGGCGAAATGGAATTATCATT
TGAAGACCGCGATGTGGTTTTACTGAAGCGTTTTTACAATGGTAGTGAGGTTCTGGCAATATTCAATTTCGGTAACGAGG
AAAGGGAAATAGCAATTCGAACTTCCGAAAGATCCGGGGTTCTTCATTACATGGAACATTTGAGCGATAGTCCCGTACAT
CTAAAGGTTAGAAACCATGAGATGCGATTAGCCCTGAATTCAAAACCTCTGGTACTTTCGAGAGTCATATACTAA

Upstream 100 bases:

>100_bases
AAAAGTGATTGTTGAACGCGAAAGAGAGCAAGAAAACGGTTAATAAGCACAAAGAAGACGATTTTGGGGGGCGATAGAGC
CCCCTTTTTTGGGAGGTAAA

Downstream 100 bases:

>100_bases
GAGGTGATTTTGTGCATTTTGGATATACCACTTATAAGATCAAACTTTACGCCATAAATTCACTGAAGGAGAAAAGAAGC
GTTGTAAAACGATTGATAAA

Product: alpha amylase catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKDTQVTAFDLAIYEIFPRMFVDKGGGAFKNISGILSELKSLGINTIWLMPIHPVGIENRKGASGSPYAVRDYFEIDSK
LGSKEDYRVFVKTAHSYGIKVFMDMVMNHCACDSVLVREHPDWILKDGQGKFGRKIAEWEDVYDLDYHNQQLVDYMVSVL
RYWVEEFDIDGYRCDVADLVPLSFWLKARHEISMIKPDFLWLSEGYENEMYRAFDITYDYDGFTNFLQYLEKKIPLMDYI
SYLNYQHRNSPKNAVKLRFLENHDQLRISGRLGVEEVLEWTAFMLMLRGVPLIYNGQEYLKGSRPDIFDYPPLYEKKNLW
FRDFLAGLLKLRSKSPALRYGEMELSFEDRDVVLLKRFYNGSEVLAIFNFGNEEREIAIRTSERSGVLHYMEHLSDSPVH
LKVRNHEMRLALNSKPLVLSRVIY

Sequences:

>Translated_424_residues
MAKDTQVTAFDLAIYEIFPRMFVDKGGGAFKNISGILSELKSLGINTIWLMPIHPVGIENRKGASGSPYAVRDYFEIDSK
LGSKEDYRVFVKTAHSYGIKVFMDMVMNHCACDSVLVREHPDWILKDGQGKFGRKIAEWEDVYDLDYHNQQLVDYMVSVL
RYWVEEFDIDGYRCDVADLVPLSFWLKARHEISMIKPDFLWLSEGYENEMYRAFDITYDYDGFTNFLQYLEKKIPLMDYI
SYLNYQHRNSPKNAVKLRFLENHDQLRISGRLGVEEVLEWTAFMLMLRGVPLIYNGQEYLKGSRPDIFDYPPLYEKKNLW
FRDFLAGLLKLRSKSPALRYGEMELSFEDRDVVLLKRFYNGSEVLAIFNFGNEEREIAIRTSERSGVLHYMEHLSDSPVH
LKVRNHEMRLALNSKPLVLSRVIY
>Mature_423_residues
AKDTQVTAFDLAIYEIFPRMFVDKGGGAFKNISGILSELKSLGINTIWLMPIHPVGIENRKGASGSPYAVRDYFEIDSKL
GSKEDYRVFVKTAHSYGIKVFMDMVMNHCACDSVLVREHPDWILKDGQGKFGRKIAEWEDVYDLDYHNQQLVDYMVSVLR
YWVEEFDIDGYRCDVADLVPLSFWLKARHEISMIKPDFLWLSEGYENEMYRAFDITYDYDGFTNFLQYLEKKIPLMDYIS
YLNYQHRNSPKNAVKLRFLENHDQLRISGRLGVEEVLEWTAFMLMLRGVPLIYNGQEYLKGSRPDIFDYPPLYEKKNLWF
RDFLAGLLKLRSKSPALRYGEMELSFEDRDVVLLKRFYNGSEVLAIFNFGNEEREIAIRTSERSGVLHYMEHLSDSPVHL
KVRNHEMRLALNSKPLVLSRVIY

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1790687, Length=200, Percent_Identity=29, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=112, Percent_Identity=29.4642857142857, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586599, Length=214, Percent_Identity=29.9065420560748, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586597, Length=209, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45549022, Length=379, Percent_Identity=21.6358839050132, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586591, Length=202, Percent_Identity=25.7425742574257, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 49713; Mature: 49582

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDTQVTAFDLAIYEIFPRMFVDKGGGAFKNISGILSELKSLGINTIWLMPIHPVGIEN
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
RKGASGSPYAVRDYFEIDSKLGSKEDYRVFVKTAHSYGIKVFMDMVMNHCACDSVLVREH
CCCCCCCCCEEHHHHHHHHHCCCCCCEEEEEEEHHHCHHHHHHHHHHHHHCCCCCHHHCC
PDWILKDGQGKFGRKIAEWEDVYDLDYHNQQLVDYMVSVLRYWVEEFDIDGYRCDVADLV
CCEEEECCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHH
PLSFWLKARHEISMIKPDFLWLSEGYENEMYRAFDITYDYDGFTNFLQYLEKKIPLMDYI
HHHHHHHHCCCCEEECCCEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHH
SYLNYQHRNSPKNAVKLRFLENHDQLRISGRLGVEEVLEWTAFMLMLRGVPLIYNGQEYL
HHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCHHHH
KGSRPDIFDYPPLYEKKNLWFRDFLAGLLKLRSKSPALRYGEMELSFEDRDVVLLKRFYN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEEECCCCEEEEEECCC
GSEVLAIFNFGNEEREIAIRTSERSGVLHYMEHLSDSPVHLKVRNHEMRLALNSKPLVLS
CCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEECCEEEEEECCCCEEHH
RVIY
HHCC
>Mature Secondary Structure 
AKDTQVTAFDLAIYEIFPRMFVDKGGGAFKNISGILSELKSLGINTIWLMPIHPVGIEN
CCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
RKGASGSPYAVRDYFEIDSKLGSKEDYRVFVKTAHSYGIKVFMDMVMNHCACDSVLVREH
CCCCCCCCCEEHHHHHHHHHCCCCCCEEEEEEEHHHCHHHHHHHHHHHHHCCCCCHHHCC
PDWILKDGQGKFGRKIAEWEDVYDLDYHNQQLVDYMVSVLRYWVEEFDIDGYRCDVADLV
CCEEEECCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHH
PLSFWLKARHEISMIKPDFLWLSEGYENEMYRAFDITYDYDGFTNFLQYLEKKIPLMDYI
HHHHHHHHCCCCEEECCCEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHH
SYLNYQHRNSPKNAVKLRFLENHDQLRISGRLGVEEVLEWTAFMLMLRGVPLIYNGQEYL
HHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCHHHH
KGSRPDIFDYPPLYEKKNLWFRDFLAGLLKLRSKSPALRYGEMELSFEDRDVVLLKRFYN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEEECCCCEEEEEECCC
GSEVLAIFNFGNEEREIAIRTSERSGVLHYMEHLSDSPVHLKVRNHEMRLALNSKPLVLS
CCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEECCEEEEEECCCCEEHH
RVIY
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]