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The map label for this gene is rimO [H]

Identifier: 239617933

GI number: 239617933

Start: 1673092

End: 1674393

Strand: Direct

Name: rimO [H]

Synonym: Kole_1561

Alternate gene names: 239617933

Gene position: 1673092-1674393 (Clockwise)

Preceding gene: 239617932

Following gene: 239617934

Centisome position: 72.68

GC content: 41.24

Gene sequence:

>1302_bases
ATGAGAATAGGAACACTCGTACTTGGTTGTCCCAAAAACATCGCCGATATGGATAATTTCAAAGGAATAATGCTCAAAAG
AGGCCACGAATTCGCTAATGGCGTGGAAAGTGCTGGTATTGTCGTTATCGATACCTGTGGGTTCATAGATGAAGCAAAAA
AAGAAAGCATAGAAGAAATCCTGAGGCTCTGCTCATTCAAAGAAGAAAAACCAGATCTTAAGGTAATCGCTGTTGGGTGC
CTTGTTCAGAGATACTATCGGGAATTAAAGGAAGGAATCCCTGAAATCGATGGGTTGATAGGAGTTACATCTCCGGAAAA
ATTGGCCGATCTTATTGAGGCAGGGAATTTTTTCTTTATTGAAAAACCGTCAACAGTTTATGATTTCAAATTTCGAACGT
ATGGCAAACCTTATTCTTATGTGAAGATTGGCGATGGTTGTGATCGTGGCTGTGCCTTTTGTTCTATCCCATCGTTCAAG
GGTTCATCACGGAGCAGACCTATTGAAGAAATAGAGAAAGAGGTTTGTTTTCTCGTTTCTGAAGGGATAAGAGAGATTGT
ACTGGTTTCACAGGATACCACACAATATGGAGTGGATATATACGGCAAGCAGGCTTTGCCGGATCTGTTGAAATCTTTGA
ACGAGATCCCTGGAGATTTCTGGATCCGGGTAATGTATTTGCATCCTGACCATCTTACTGAAGGAATCATTAATTCAATT
TTGAAACTTGAAAAAGTAGTGAATTATTTCGATGTACCAGTACAAAGCGGTTCTGACTATATTCTTAAACGCATGGGAAG
AACAAAAAAAGCTGGAGAATTGAAAGCCATGTTTGAAAAGATAAGGGCGAAAGAATCCGATGTGACTTTGCGAACCACGA
TAATGGTGGGATTTCCCGGGGAGACACGAAGGACTTTCGAAGAGACGTTGGAGTTTGTGAATGATGTTAAGTTTGATAAG
CTTGGTGGATTTGTATATTCTCCTGAGGAAGGAACACCAGCATATTCATTTAAAATGACTATTGATGAAAATATGGCCCG
TCAATACCTTGATGAACTCCTGGATTTGCAGGATGAGATATCCTATGAACTCAATCAACGACACTTTGGTAAGGTACTCA
AGGTTCTTATAGAGGAAAATACTGAAGAAGTCATGATTGGCAGGAGCCCGCACTTTGCTCCCGAAATCGACGGCAGCATC
TTTCTCAAAAGAGCCGATGAAGCGGGAGAGTTTGTGATGTGTCGTATAACCGGGGTTTACGAGCATGACCTGGAAGGTGT
GATTGTGGATGAATCTGCCTAA

Upstream 100 bases:

>100_bases
CATTGGATTATACCTACCCGAATTACAAAGATCAGGAATATATTCAGGAATTGACCGGTATCAGAGAAATTTATCTGAAA
AAGAGAAAGGAATTTTATAG

Downstream 100 bases:

>100_bases
TATATTGACGTTTTCAAGAATGTTATTGACATTTCCTGTGGTAGCATTACTTACAGTAGAAAATAGGGTTTCGGTTATTA
TTTCCGTTCTTTTATTTACG

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRIGTLVLGCPKNIADMDNFKGIMLKRGHEFANGVESAGIVVIDTCGFIDEAKKESIEEILRLCSFKEEKPDLKVIAVGC
LVQRYYRELKEGIPEIDGLIGVTSPEKLADLIEAGNFFFIEKPSTVYDFKFRTYGKPYSYVKIGDGCDRGCAFCSIPSFK
GSSRSRPIEEIEKEVCFLVSEGIREIVLVSQDTTQYGVDIYGKQALPDLLKSLNEIPGDFWIRVMYLHPDHLTEGIINSI
LKLEKVVNYFDVPVQSGSDYILKRMGRTKKAGELKAMFEKIRAKESDVTLRTTIMVGFPGETRRTFEETLEFVNDVKFDK
LGGFVYSPEEGTPAYSFKMTIDENMARQYLDELLDLQDEISYELNQRHFGKVLKVLIEENTEEVMIGRSPHFAPEIDGSI
FLKRADEAGEFVMCRITGVYEHDLEGVIVDESA

Sequences:

>Translated_433_residues
MRIGTLVLGCPKNIADMDNFKGIMLKRGHEFANGVESAGIVVIDTCGFIDEAKKESIEEILRLCSFKEEKPDLKVIAVGC
LVQRYYRELKEGIPEIDGLIGVTSPEKLADLIEAGNFFFIEKPSTVYDFKFRTYGKPYSYVKIGDGCDRGCAFCSIPSFK
GSSRSRPIEEIEKEVCFLVSEGIREIVLVSQDTTQYGVDIYGKQALPDLLKSLNEIPGDFWIRVMYLHPDHLTEGIINSI
LKLEKVVNYFDVPVQSGSDYILKRMGRTKKAGELKAMFEKIRAKESDVTLRTTIMVGFPGETRRTFEETLEFVNDVKFDK
LGGFVYSPEEGTPAYSFKMTIDENMARQYLDELLDLQDEISYELNQRHFGKVLKVLIEENTEEVMIGRSPHFAPEIDGSI
FLKRADEAGEFVMCRITGVYEHDLEGVIVDESA
>Mature_433_residues
MRIGTLVLGCPKNIADMDNFKGIMLKRGHEFANGVESAGIVVIDTCGFIDEAKKESIEEILRLCSFKEEKPDLKVIAVGC
LVQRYYRELKEGIPEIDGLIGVTSPEKLADLIEAGNFFFIEKPSTVYDFKFRTYGKPYSYVKIGDGCDRGCAFCSIPSFK
GSSRSRPIEEIEKEVCFLVSEGIREIVLVSQDTTQYGVDIYGKQALPDLLKSLNEIPGDFWIRVMYLHPDHLTEGIINSI
LKLEKVVNYFDVPVQSGSDYILKRMGRTKKAGELKAMFEKIRAKESDVTLRTTIMVGFPGETRRTFEETLEFVNDVKFDK
LGGFVYSPEEGTPAYSFKMTIDENMARQYLDELLDLQDEISYELNQRHFGKVLKVLIEENTEEVMIGRSPHFAPEIDGSI
FLKRADEAGEFVMCRITGVYEHDLEGVIVDESA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=468, Percent_Identity=22.8632478632479, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI28872782, Length=468, Percent_Identity=22.8632478632479, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI93277076, Length=430, Percent_Identity=24.6511627906977, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=32.5056433408578, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=28.6036036036036, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=25.3731343283582, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=287, Percent_Identity=29.2682926829268, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=23.1578947368421, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=26.8292682926829, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48984; Mature: 48984

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGTLVLGCPKNIADMDNFKGIMLKRGHEFANGVESAGIVVIDTCGFIDEAKKESIEEI
CCCCEEEEECCCCCCCCCCCCCCEEECCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
LRLCSFKEEKPDLKVIAVGCLVQRYYRELKEGIPEIDGLIGVTSPEKLADLIEAGNFFFI
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCCEEEE
EKPSTVYDFKFRTYGKPYSYVKIGDGCDRGCAFCSIPSFKGSSRSRPIEEIEKEVCFLVS
ECCCEEEEEEEEECCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
EGIREIVLVSQDTTQYGVDIYGKQALPDLLKSLNEIPGDFWIRVMYLHPDHLTEGIINSI
HCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHH
LKLEKVVNYFDVPVQSGSDYILKRMGRTKKAGELKAMFEKIRAKESDVTLRTTIMVGFPG
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCC
ETRRTFEETLEFVNDVKFDKLGGFVYSPEEGTPAYSFKMTIDENMARQYLDELLDLQDEI
CHHHHHHHHHHHHHCCCHHHHCCEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHH
SYELNQRHFGKVLKVLIEENTEEVMIGRSPHFAPEIDGSIFLKRADEAGEFVMCRITGVY
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECEE
EHDLEGVIVDESA
ECCCCEEEEECCC
>Mature Secondary Structure
MRIGTLVLGCPKNIADMDNFKGIMLKRGHEFANGVESAGIVVIDTCGFIDEAKKESIEEI
CCCCEEEEECCCCCCCCCCCCCCEEECCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
LRLCSFKEEKPDLKVIAVGCLVQRYYRELKEGIPEIDGLIGVTSPEKLADLIEAGNFFFI
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCCEEEE
EKPSTVYDFKFRTYGKPYSYVKIGDGCDRGCAFCSIPSFKGSSRSRPIEEIEKEVCFLVS
ECCCEEEEEEEEECCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
EGIREIVLVSQDTTQYGVDIYGKQALPDLLKSLNEIPGDFWIRVMYLHPDHLTEGIINSI
HCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHH
LKLEKVVNYFDVPVQSGSDYILKRMGRTKKAGELKAMFEKIRAKESDVTLRTTIMVGFPG
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCC
ETRRTFEETLEFVNDVKFDKLGGFVYSPEEGTPAYSFKMTIDENMARQYLDELLDLQDEI
CHHHHHHHHHHHHHCCCHHHHCCEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHH
SYELNQRHFGKVLKVLIEENTEEVMIGRSPHFAPEIDGSIFLKRADEAGEFVMCRITGVY
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECEE
EHDLEGVIVDESA
ECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]