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The map label for this gene is miaB [H]

Identifier: 239617912

GI number: 239617912

Start: 1650262

End: 1651584

Strand: Direct

Name: miaB [H]

Synonym: Kole_1540

Alternate gene names: 239617912

Gene position: 1650262-1651584 (Clockwise)

Preceding gene: 239617911

Following gene: 239617915

Centisome position: 71.68

GC content: 41.72

Gene sequence:

>1323_bases
ATGAAAATAGCTTTCAGAACCTTTGGATGCCAAATGAATGTGAACGATACGGAAACTATGGCCGGCATTCTCAAATCGAA
TGGGTATGTGATTGTCGATAATGAAGAAGAAGCTGATGTTGTTATCGTTAACACCTGTGCGGTTAGAGAGAAGGCCGAGA
AAAAACTTTATGGAAAACTTGGAAGACTGAGATCCCTGAAGAAGAAAAACAGGAACCTGATAATCGGCGTTTCCGGTTGT
GTGGCCGAGAAAGAAAAAGAGGCCCTTTTGAAGCGCGAAGAGGTGAACTTTGTCTTCGGGACTCGAAGTATATCAAGGGT
TAATGAATTTTTGGAGAGAGCTATTAAGGGAGAGCGCTTTGTCGAGTTATCGGATTTCATAGACGAAATTAATTCAAGCA
CCCCACGATTAAGAACTAGTCGTCATCATGCTTGGGTGACGATAATCTATGGCTGCAACAAATTTTGTTCGTATTGTATC
GTTCCTTATACTAGAGGGAGAGAAAAAAGCAGGTCCATGGAAGACATACTCAATGAAGTCAAAAGGCTCGCCTCAAAGGG
CTATAGAGAGGTAACATATCTAGGGCAAAATGTGGATTCTTACGGCAAAGATCTTGCTGATGGCAGCACCCTTGCGAAGT
TGATAAGAGAGACCCTCAAGATAGAGCAGATAGAAAGAATCTGGTATCTGACATCTTACCCGAAAGATTTCAGCGACGAG
TTGATCGATGTTATAGCAACCTCCAAACGTGTCTCGCGTTCTATTCATTTACCAATACAATCCGGGAGTAATAGAATACT
GAAAGCAATGAATAGAGGTTACACACGTGAAGAGTATCTAGATCTTATCCGCAGGATCAGGACAAAGGTACCCGATGCTT
CAATAAGCACCGATATCATTGTCGGCTTTCCGGGCGAAAGTGAAGACGATTATCTCCAGACCAAATCGCTGCTTGAAGAG
GTTATTTTCGAAAGGGTTAACCTGGCAATCTATTCCCCACGGGAGGGAACGATATCAGCGAAGTATATGAAAGATGACGT
TCCTCATGAAATTAAGGTGAGAAGACTTCAGGAGCTTCTGGAGCTTCAAAAATGTTTGAATAGAAAAATCAATTCGGAGT
ATATCGGGAAAACAGTCGAAATAATCGTCGAAGCCAAACACAAAGATGGGATGCTTTATGGTAGAACGATCAACAACAAA
ATCGTTTTCTTCCACGGTTCTGAAGAGTTGATAGGTTCAAAAGCGAAAATAACCATTGAAAAGACAACAGCCGGCCCTCT
TTATGGCCAGCTTGTTGAAAATGAGCTCATAACTAAAAAATAA

Upstream 100 bases:

>100_bases
AAAGAAGAACTCTGAGGATTTCTGAAGATGGTAAAAGCGACGGCGTGGATGCCGTCGCTTTTTTGTAACGGTTGAAAAAA
CACATAGAGGTGATATCACG

Downstream 100 bases:

>100_bases
CAAAAGTAAATAGATTTATCCGTTCATGAGATCGTGGTATTTCTTATCGATCTCAGCTTTTTTGTTTTGTACCACAGCAA
GTTTTTCCCTGCAGAGCATT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKIAFRTFGCQMNVNDTETMAGILKSNGYVIVDNEEEADVVIVNTCAVREKAEKKLYGKLGRLRSLKKKNRNLIIGVSGC
VAEKEKEALLKREEVNFVFGTRSISRVNEFLERAIKGERFVELSDFIDEINSSTPRLRTSRHHAWVTIIYGCNKFCSYCI
VPYTRGREKSRSMEDILNEVKRLASKGYREVTYLGQNVDSYGKDLADGSTLAKLIRETLKIEQIERIWYLTSYPKDFSDE
LIDVIATSKRVSRSIHLPIQSGSNRILKAMNRGYTREEYLDLIRRIRTKVPDASISTDIIVGFPGESEDDYLQTKSLLEE
VIFERVNLAIYSPREGTISAKYMKDDVPHEIKVRRLQELLELQKCLNRKINSEYIGKTVEIIVEAKHKDGMLYGRTINNK
IVFFHGSEELIGSKAKITIEKTTAGPLYGQLVENELITKK

Sequences:

>Translated_440_residues
MKIAFRTFGCQMNVNDTETMAGILKSNGYVIVDNEEEADVVIVNTCAVREKAEKKLYGKLGRLRSLKKKNRNLIIGVSGC
VAEKEKEALLKREEVNFVFGTRSISRVNEFLERAIKGERFVELSDFIDEINSSTPRLRTSRHHAWVTIIYGCNKFCSYCI
VPYTRGREKSRSMEDILNEVKRLASKGYREVTYLGQNVDSYGKDLADGSTLAKLIRETLKIEQIERIWYLTSYPKDFSDE
LIDVIATSKRVSRSIHLPIQSGSNRILKAMNRGYTREEYLDLIRRIRTKVPDASISTDIIVGFPGESEDDYLQTKSLLEE
VIFERVNLAIYSPREGTISAKYMKDDVPHEIKVRRLQELLELQKCLNRKINSEYIGKTVEIIVEAKHKDGMLYGRTINNK
IVFFHGSEELIGSKAKITIEKTTAGPLYGQLVENELITKK
>Mature_440_residues
MKIAFRTFGCQMNVNDTETMAGILKSNGYVIVDNEEEADVVIVNTCAVREKAEKKLYGKLGRLRSLKKKNRNLIIGVSGC
VAEKEKEALLKREEVNFVFGTRSISRVNEFLERAIKGERFVELSDFIDEINSSTPRLRTSRHHAWVTIIYGCNKFCSYCI
VPYTRGREKSRSMEDILNEVKRLASKGYREVTYLGQNVDSYGKDLADGSTLAKLIRETLKIEQIERIWYLTSYPKDFSDE
LIDVIATSKRVSRSIHLPIQSGSNRILKAMNRGYTREEYLDLIRRIRTKVPDASISTDIIVGFPGESEDDYLQTKSLLEE
VIFERVNLAIYSPREGTISAKYMKDDVPHEIKVRRLQELLELQKCLNRKINSEYIGKTVEIIVEAKHKDGMLYGRTINNK
IVFFHGSEELIGSKAKITIEKTTAGPLYGQLVENELITKK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=439, Percent_Identity=39.1799544419134, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI28872784, Length=439, Percent_Identity=39.1799544419134, Blast_Score=304, Evalue=9e-83,
Organism=Homo sapiens, GI93277076, Length=368, Percent_Identity=29.0760869565217, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=40.7158836689038, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=28.1879194630872, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=33.9092872570194, Blast_Score=260, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=275, Percent_Identity=25.8181818181818, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.6443418013857, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI19922432, Length=449, Percent_Identity=26.5033407572383, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50379; Mature: 50379

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFRTFGCQMNVNDTETMAGILKSNGYVIVDNEEEADVVIVNTCAVREKAEKKLYGKL
CEEEEEEECCEECCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
GRLRSLKKKNRNLIIGVSGCVAEKEKEALLKREEVNFVFGTRSISRVNEFLERAIKGERF
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCE
VELSDFIDEINSSTPRLRTSRHHAWVTIIYGCNKFCSYCIVPYTRGREKSRSMEDILNEV
EEHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHCEEECCCCCCCCHHHHHHHHHHHH
KRLASKGYREVTYLGQNVDSYGKDLADGSTLAKLIRETLKIEQIERIWYLTSYPKDFSDE
HHHHHCCCCEEHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHH
LIDVIATSKRVSRSIHLPIQSGSNRILKAMNRGYTREEYLDLIRRIRTKVPDASISTDII
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
VGFPGESEDDYLQTKSLLEEVIFERVNLAIYSPREGTISAKYMKDDVPHEIKVRRLQELL
EECCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEEHHHCCCCCHHHHHHHHHHHH
ELQKCLNRKINSEYIGKTVEIIVEAKHKDGMLYGRTINNKIVFFHGSEELIGSKAKITIE
HHHHHHCCCCCHHHCCCEEEEEEEECCCCCEEEEEEECCEEEEEECCHHHHCCCEEEEEE
KTTAGPLYGQLVENELITKK
ECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIAFRTFGCQMNVNDTETMAGILKSNGYVIVDNEEEADVVIVNTCAVREKAEKKLYGKL
CEEEEEEECCEECCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHH
GRLRSLKKKNRNLIIGVSGCVAEKEKEALLKREEVNFVFGTRSISRVNEFLERAIKGERF
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCE
VELSDFIDEINSSTPRLRTSRHHAWVTIIYGCNKFCSYCIVPYTRGREKSRSMEDILNEV
EEHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHCEEECCCCCCCCHHHHHHHHHHHH
KRLASKGYREVTYLGQNVDSYGKDLADGSTLAKLIRETLKIEQIERIWYLTSYPKDFSDE
HHHHHCCCCEEHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHH
LIDVIATSKRVSRSIHLPIQSGSNRILKAMNRGYTREEYLDLIRRIRTKVPDASISTDII
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
VGFPGESEDDYLQTKSLLEEVIFERVNLAIYSPREGTISAKYMKDDVPHEIKVRRLQELL
EECCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEEHHHCCCCCHHHHHHHHHHHH
ELQKCLNRKINSEYIGKTVEIIVEAKHKDGMLYGRTINNKIVFFHGSEELIGSKAKITIE
HHHHHHCCCCCHHHCCCEEEEEEEECCCCCEEEEEEECCEEEEEECCHHHHCCCEEEEEE
KTTAGPLYGQLVENELITKK
ECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]