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The map label for this gene is tyrS [H]

Identifier: 239617872

GI number: 239617872

Start: 1607377

End: 1608570

Strand: Direct

Name: tyrS [H]

Synonym: Kole_1499

Alternate gene names: 239617872

Gene position: 1607377-1608570 (Clockwise)

Preceding gene: 239617871

Following gene: 239617873

Centisome position: 69.82

GC content: 42.55

Gene sequence:

>1194_bases
TTGGAAGTACCGGAAAAACAATTGCAGATACTTAAAAGGAACTCCGTTTCCTTGATTTCGGAGGAAGAATTAATTGAAAG
ATTGAAGCTCGGAAGGCCGCTTCGAGTCAAGCTTGGAGTTGATCCTTCAAGACCGGATCTTCATCTTGGCCATGCAGTTG
TTCTCAGGAAACTCCGGGAATTTCAGGACCTCGGCCATCACGTTATTTTGATAATAGGTGATTTTACGGCTCGAATTGGC
GACCCTTCCGGTCGCTCGAAAACCCGCCCGATGCTCTCAAAAGAAGAAGCGAAACAGAATGCCATCACCTACAGTCAGCA
AGCCTTTAAAATCCTCGATCGAGATAAAACAGAGATAAAGTTTAATTCTGAATGGCTGGATGGTATGAGTTTTGAAGATG
TGATACGGCTTTCGGCTAAGTATACCGTTGCGAGGATGCTTGAAAGAAATGATTTCGAGAAGCGGTATAAGAATAACGAA
CCTATAAGCATAGCAGAGTTTCTTTACCCGCTTGCCCAGGCTTATGATTCGGTGGTGGTCAAAGCGGATGTGGAGATCGG
CGGTGACGACCAGTTCTTCAATTTTGTTGTTGCGCGTAAGATTCAGGAAGAATATGGTTTGCAACCCCAGGTCATTCTCA
CCATGCCTCTCATAGAGGGAACCGACGGCAATTTGAAAATGTCCAAGAGCTATGGCAATTATGTTGCCTTTGAAGACCCT
CCAGAGGAAATGTTTGGAAAACTCATGTCGATTCCCGATACCTTAATAATTAAATATATGAGGTTGCTCACAGACATTCC
TGAAGAGCGGATTCTCCAATACGAAAGGGATATAAAATCAAGGAAAATCAATCCACGGGATGTAAAAATGTTGCTGGCTT
TTGAGATAACAAGATTTTTCCACGGTGAAGAAGCTGCGGTGCGTGCAAGGGAGAATTTCATAAAAGTTTTCAGAAAAAAG
GAACTTCCCAGTGAAATACCTGAAATTAAAGTAAAAGAATCCGAACTATCTCTTGTAGATCTGATGGTTAAATATGCTAA
TATTTCATCAAAAAGCGAGGCCAGACGGTTGATTTCTCAAGGCGGTGTTCGGCTGAACAACACGGTGATAAAAGATATTC
ATGCTAAGGTCGCAGTATCCAATGGGGACATACTACGTATCGGTAAACGTAGATTCTTCAAGATTTCGTGCTAA

Upstream 100 bases:

>100_bases
GCCGGGAAGATCACCCTCGGCCTTTCCATCGCTTTTATGGTGAACAGCGTTATTTTGACATTTGTTATCTCAAAAGCATT
CCCGTCGTAAGGTGATAATC

Downstream 100 bases:

>100_bases
ACTTATAGAAATCAAAACTTGAACAAGTTGGCCTGCATACTGTATAATAAATTCGTGCTTTAGGCAGATTTACAACTTTC
CGAAAGGGGGTAGGTTCCAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MEVPEKQLQILKRNSVSLISEEELIERLKLGRPLRVKLGVDPSRPDLHLGHAVVLRKLREFQDLGHHVILIIGDFTARIG
DPSGRSKTRPMLSKEEAKQNAITYSQQAFKILDRDKTEIKFNSEWLDGMSFEDVIRLSAKYTVARMLERNDFEKRYKNNE
PISIAEFLYPLAQAYDSVVVKADVEIGGDDQFFNFVVARKIQEEYGLQPQVILTMPLIEGTDGNLKMSKSYGNYVAFEDP
PEEMFGKLMSIPDTLIIKYMRLLTDIPEERILQYERDIKSRKINPRDVKMLLAFEITRFFHGEEAAVRARENFIKVFRKK
ELPSEIPEIKVKESELSLVDLMVKYANISSKSEARRLISQGGVRLNNTVIKDIHAKVAVSNGDILRIGKRRFFKISC

Sequences:

>Translated_397_residues
MEVPEKQLQILKRNSVSLISEEELIERLKLGRPLRVKLGVDPSRPDLHLGHAVVLRKLREFQDLGHHVILIIGDFTARIG
DPSGRSKTRPMLSKEEAKQNAITYSQQAFKILDRDKTEIKFNSEWLDGMSFEDVIRLSAKYTVARMLERNDFEKRYKNNE
PISIAEFLYPLAQAYDSVVVKADVEIGGDDQFFNFVVARKIQEEYGLQPQVILTMPLIEGTDGNLKMSKSYGNYVAFEDP
PEEMFGKLMSIPDTLIIKYMRLLTDIPEERILQYERDIKSRKINPRDVKMLLAFEITRFFHGEEAAVRARENFIKVFRKK
ELPSEIPEIKVKESELSLVDLMVKYANISSKSEARRLISQGGVRLNNTVIKDIHAKVAVSNGDILRIGKRRFFKISC
>Mature_397_residues
MEVPEKQLQILKRNSVSLISEEELIERLKLGRPLRVKLGVDPSRPDLHLGHAVVLRKLREFQDLGHHVILIIGDFTARIG
DPSGRSKTRPMLSKEEAKQNAITYSQQAFKILDRDKTEIKFNSEWLDGMSFEDVIRLSAKYTVARMLERNDFEKRYKNNE
PISIAEFLYPLAQAYDSVVVKADVEIGGDDQFFNFVVARKIQEEYGLQPQVILTMPLIEGTDGNLKMSKSYGNYVAFEDP
PEEMFGKLMSIPDTLIIKYMRLLTDIPEERILQYERDIKSRKINPRDVKMLLAFEITRFFHGEEAAVRARENFIKVFRKK
ELPSEIPEIKVKESELSLVDLMVKYANISSKSEARRLISQGGVRLNNTVIKDIHAKVAVSNGDILRIGKRRFFKISC

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=420, Percent_Identity=28.0952380952381, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787925, Length=325, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=290, Percent_Identity=28.6206896551724, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=433, Percent_Identity=28.4064665127021, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130383, Length=401, Percent_Identity=25.9351620947631, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45749; Mature: 45749

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVPEKQLQILKRNSVSLISEEELIERLKLGRPLRVKLGVDPSRPDLHLGHAVVLRKLRE
CCCCHHHHHHHHCCCHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQDLGHHVILIIGDFTARIGDPSGRSKTRPMLSKEEAKQNAITYSQQAFKILDRDKTEIK
HHHCCCEEEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
FNSEWLDGMSFEDVIRLSAKYTVARMLERNDFEKRYKNNEPISIAEFLYPLAQAYDSVVV
ECCHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHCCEEE
KADVEIGGDDQFFNFVVARKIQEEYGLQPQVILTMPLIEGTDGNLKMSKSYGNYVAFEDP
EEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEECCCCCEEECCCC
PEEMFGKLMSIPDTLIIKYMRLLTDIPEERILQYERDIKSRKINPRDVKMLLAFEITRFF
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HGEEAAVRARENFIKVFRKKELPSEIPEIKVKESELSLVDLMVKYANISSKSEARRLISQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GGVRLNNTVIKDIHAKVAVSNGDILRIGKRRFFKISC
CCCEECCHHHHHHHHEEEECCCCEEEECCCEEEEECC
>Mature Secondary Structure
MEVPEKQLQILKRNSVSLISEEELIERLKLGRPLRVKLGVDPSRPDLHLGHAVVLRKLRE
CCCCHHHHHHHHCCCHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQDLGHHVILIIGDFTARIGDPSGRSKTRPMLSKEEAKQNAITYSQQAFKILDRDKTEIK
HHHCCCEEEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
FNSEWLDGMSFEDVIRLSAKYTVARMLERNDFEKRYKNNEPISIAEFLYPLAQAYDSVVV
ECCHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHCCEEE
KADVEIGGDDQFFNFVVARKIQEEYGLQPQVILTMPLIEGTDGNLKMSKSYGNYVAFEDP
EEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEECCCCCEEECCCC
PEEMFGKLMSIPDTLIIKYMRLLTDIPEERILQYERDIKSRKINPRDVKMLLAFEITRFF
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HGEEAAVRARENFIKVFRKKELPSEIPEIKVKESELSLVDLMVKYANISSKSEARRLISQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GGVRLNNTVIKDIHAKVAVSNGDILRIGKRRFFKISC
CCCEECCHHHHHHHHEEEECCCCEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]