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The map label for this gene is yqjC [H]

Identifier: 239617745

GI number: 239617745

Start: 1454422

End: 1454829

Strand: Reverse

Name: yqjC [H]

Synonym: Kole_1369

Alternate gene names: 239617745

Gene position: 1454829-1454422 (Counterclockwise)

Preceding gene: 239617746

Following gene: 239617744

Centisome position: 63.2

GC content: 42.4

Gene sequence:

>408_bases
TTGAAAATCGATCGTATCGACCATATCGGTATCGTTGTTCGCTCAATAGACGAAAAACTTCCGCTTTACAGGGACTTCCT
CGGATTGGAATTTGGAGGAATAGAAGAACTACCGGAAAGGGGGCTAAAGGTTGCATTCTTAAAAGTTGGAACCACGAGAA
TAGAATTACTAGAACCTTTGAATGAGAAAAGTCAAATATCGAAATTCCTTGAAAAAAAGGGTGAAGGCATCCATCATATA
GCTTATCACGTCGATAACATCAATGAAGCACTCGAAAAGGCTAAAAACGGTGGTTTTAAACCTCTCAGCGATGAACCTCA
GCCGGGTGCCGGTGGAACAAAAGTGATCTTCCTGCACCCAAAAACCACCGGCGGTGTTCTCATAGAAATGGTAGAAGGTC
AACACTAA

Upstream 100 bases:

>100_bases
TCAACAGTATCTGTGAAATCATACAGGAAAGATTCCTGGAAGATGAAAAACTGGACACTTTGTTGGAATTCATCAAAAAC
TTCGATAGGGGGGAAGAAGC

Downstream 100 bases:

>100_bases
TGGGGGGTGATGAGTTGAACGAAAAACTCGAGAAGTTCAAAAAGCTAGATGAAAAAATAACCCTTGGCGGTGGAAAAGAG
AGAATAGAGAAACAGCATGC

Product: methylmalonyl-CoA epimerase

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MKIDRIDHIGIVVRSIDEKLPLYRDFLGLEFGGIEELPERGLKVAFLKVGTTRIELLEPLNEKSQISKFLEKKGEGIHHI
AYHVDNINEALEKAKNGGFKPLSDEPQPGAGGTKVIFLHPKTTGGVLIEMVEGQH

Sequences:

>Translated_135_residues
MKIDRIDHIGIVVRSIDEKLPLYRDFLGLEFGGIEELPERGLKVAFLKVGTTRIELLEPLNEKSQISKFLEKKGEGIHHI
AYHVDNINEALEKAKNGGFKPLSDEPQPGAGGTKVIFLHPKTTGGVLIEMVEGQH
>Mature_135_residues
MKIDRIDHIGIVVRSIDEKLPLYRDFLGLEFGGIEELPERGLKVAFLKVGTTRIELLEPLNEKSQISKFLEKKGEGIHHI
AYHVDNINEALEKAKNGGFKPLSDEPQPGAGGTKVIFLHPKTTGGVLIEMVEGQH

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=131, Percent_Identity=41.9847328244275, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17506387, Length=129, Percent_Identity=37.984496124031, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14992; Mature: 14992

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDRIDHIGIVVRSIDEKLPLYRDFLGLEFGGIEELPERGLKVAFLKVGTTRIELLEPL
CCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHCC
NEKSQISKFLEKKGEGIHHIAYHVDNINEALEKAKNGGFKPLSDEPQPGAGGTKVIFLHP
CCHHHHHHHHHHHCCCEEEEHEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
KTTGGVLIEMVEGQH
CCCCCEEEEECCCCC
>Mature Secondary Structure
MKIDRIDHIGIVVRSIDEKLPLYRDFLGLEFGGIEELPERGLKVAFLKVGTTRIELLEPL
CCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHCC
NEKSQISKFLEKKGEGIHHIAYHVDNINEALEKAKNGGFKPLSDEPQPGAGGTKVIFLHP
CCHHHHHHHHHHHCCCEEEEHEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
KTTGGVLIEMVEGQH
CCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]