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The map label for this gene is phoA [H]

Identifier: 239617735

GI number: 239617735

Start: 1442031

End: 1443359

Strand: Direct

Name: phoA [H]

Synonym: Kole_1359

Alternate gene names: 239617735

Gene position: 1442031-1443359 (Clockwise)

Preceding gene: 239617733

Following gene: 239617736

Centisome position: 62.64

GC content: 45.22

Gene sequence:

>1329_bases
ATGCGAAGGTTTTTGCTTGTCTTTGTTCTTTTGCTTATAGTTTCATTGCTCGTTGCTTCTGGTGTTAAGAACGTTATCTA
CATGATTGGTGACGGTATGGGAATTAACCATGTCCTTTTGACAAGTTATCTTGAGGGTAGGGAACTTTACATGCTTCAGG
CTCCCTACACAGGGTTTGTCCTGACTTTTTCAGCAAACAGCAATGTTCCAGATTCAGCGTCTGCTGGAACAGCGCTAGCA
TCTGGCTACAAAACCAACAACGGAATGATCGGCATGCTTCCAGACGGAACAGTAATCACCAACATCGCTGAGATCGCGGC
TCAGTATGGTATCAAGACAGGAATTGTTGCCACCTGCCGTATAACTCACGCAACTCCAGCTGCTTTCTACGGTCACGTAA
GTTCAAGAAGAGATGAAAATACGCTAGCTGAGCAGCTCGTAGACAGTTCTTTGACCGTTGCTTTTGGTGGCGGTTGGAGA
CATTTTGTCCCAACGGGTGGCAAAAGGAAAGACGGGAAAGATCTTATAGCAATAGCTAAAGCCAATGGTTTCGACTACAT
CACGACAAAAACAGAGTTGTTGAATTACGATGGTGACAGAGTCCTCGGACTCTTCAGCTACAGTCATCTCAAAGCCGCTT
CCGAAAGAACTGGAGAAGAACCAATGCTTCCGGAAATGGTTGAGAAAGCTCTCGAGATACTTTCAAAAGACGATTCTCCA
TTCTTCCTGATGGTCGAAGGTTCTCAGATTGACTGGGAAGCCCACGGAAACGATGTTTACGGTCTATGGAAAGAAATCGT
CGAATTCGACAATGCTGTGAAAGTCGCTCTTGATTTTGCAAAGGAACACCCGAATACACTCATCGTTATTACTGCAGACC
ATGAAACAGGCGGTCTCGGACTCTCCACAGGCGGATACACAATGAATCTCGAAAAGATAAGGAAGGATTACACAAAAACC
GCTGATTGGATAGTTTCTAATTTCGGTGATTCCGATGAGGAACTCAAGGAAGCTGTCAAGACATACTTCAATGTTGAGCT
CACCGATGAAGACCTTGCATATTACCATGAAGAAAAAGCCAAATCTTTCAACGGTGCAGGAAATGCTATCGGGAGAATCC
TCAGCAACAAAGCCCAGATAGGCTGGACAACACATGACCACACAGGAGGAGCTGTCCCGATATTTGCCTTCGGTCCAGGT
GCTGAACTCTTTAGTGGCGTTCATGACAACACCGAGATTCCAAATTTGATTGCTTATCTCCTTGGAATCGGTAAGGTCAG
TGATGTTGAGTATGGTATTGAAGATGTCCCGTTGGTTGGTGGACATTGA

Upstream 100 bases:

>100_bases
TAGCCCATAAATACCGGTAGTTGTTATGAGATATTCTGTAATAAAACTATGCGATAGTGGTATACTTTTAATGCCACATT
CATTAAAAGGGGGTTTGTTT

Downstream 100 bases:

>100_bases
AAATAATTGAAAGATTTTAGGGATTTAGCGCCCCGAGAAGGGGCGCTTTGTTTTGTATGTAATATATAATTGTTTTTAGG
AATCATTCTCAGCTTTTCTA

Product: Alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRRFLLVFVLLLIVSLLVASGVKNVIYMIGDGMGINHVLLTSYLEGRELYMLQAPYTGFVLTFSANSNVPDSASAGTALA
SGYKTNNGMIGMLPDGTVITNIAEIAAQYGIKTGIVATCRITHATPAAFYGHVSSRRDENTLAEQLVDSSLTVAFGGGWR
HFVPTGGKRKDGKDLIAIAKANGFDYITTKTELLNYDGDRVLGLFSYSHLKAASERTGEEPMLPEMVEKALEILSKDDSP
FFLMVEGSQIDWEAHGNDVYGLWKEIVEFDNAVKVALDFAKEHPNTLIVITADHETGGLGLSTGGYTMNLEKIRKDYTKT
ADWIVSNFGDSDEELKEAVKTYFNVELTDEDLAYYHEEKAKSFNGAGNAIGRILSNKAQIGWTTHDHTGGAVPIFAFGPG
AELFSGVHDNTEIPNLIAYLLGIGKVSDVEYGIEDVPLVGGH

Sequences:

>Translated_442_residues
MRRFLLVFVLLLIVSLLVASGVKNVIYMIGDGMGINHVLLTSYLEGRELYMLQAPYTGFVLTFSANSNVPDSASAGTALA
SGYKTNNGMIGMLPDGTVITNIAEIAAQYGIKTGIVATCRITHATPAAFYGHVSSRRDENTLAEQLVDSSLTVAFGGGWR
HFVPTGGKRKDGKDLIAIAKANGFDYITTKTELLNYDGDRVLGLFSYSHLKAASERTGEEPMLPEMVEKALEILSKDDSP
FFLMVEGSQIDWEAHGNDVYGLWKEIVEFDNAVKVALDFAKEHPNTLIVITADHETGGLGLSTGGYTMNLEKIRKDYTKT
ADWIVSNFGDSDEELKEAVKTYFNVELTDEDLAYYHEEKAKSFNGAGNAIGRILSNKAQIGWTTHDHTGGAVPIFAFGPG
AELFSGVHDNTEIPNLIAYLLGIGKVSDVEYGIEDVPLVGGH
>Mature_442_residues
MRRFLLVFVLLLIVSLLVASGVKNVIYMIGDGMGINHVLLTSYLEGRELYMLQAPYTGFVLTFSANSNVPDSASAGTALA
SGYKTNNGMIGMLPDGTVITNIAEIAAQYGIKTGIVATCRITHATPAAFYGHVSSRRDENTLAEQLVDSSLTVAFGGGWR
HFVPTGGKRKDGKDLIAIAKANGFDYITTKTELLNYDGDRVLGLFSYSHLKAASERTGEEPMLPEMVEKALEILSKDDSP
FFLMVEGSQIDWEAHGNDVYGLWKEIVEFDNAVKVALDFAKEHPNTLIVITADHETGGLGLSTGGYTMNLEKIRKDYTKT
ADWIVSNFGDSDEELKEAVKTYFNVELTDEDLAYYHEEKAKSFNGAGNAIGRILSNKAQIGWTTHDHTGGAVPIFAFGPG
AELFSGVHDNTEIPNLIAYLLGIGKVSDVEYGIEDVPLVGGH

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=454, Percent_Identity=28.4140969162996, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI157266292, Length=452, Percent_Identity=28.5398230088496, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI94721246, Length=454, Percent_Identity=27.7533039647577, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI116734717, Length=463, Percent_Identity=29.5896328293737, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI294660770, Length=457, Percent_Identity=29.1028446389497, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI294660772, Length=425, Percent_Identity=28.9411764705882, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI48994877, Length=380, Percent_Identity=28.9473684210526, Blast_Score=138, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6320689, Length=492, Percent_Identity=32.1138211382114, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21355981, Length=338, Percent_Identity=34.9112426035503, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI85725200, Length=458, Percent_Identity=32.0960698689956, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI85815833, Length=458, Percent_Identity=32.0960698689956, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21355149, Length=457, Percent_Identity=30.4157549234136, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355151, Length=455, Percent_Identity=29.6703296703297, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI19921912, Length=470, Percent_Identity=31.063829787234, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18859923, Length=435, Percent_Identity=28.9655172413793, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24585213, Length=470, Percent_Identity=30.4255319148936, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358067, Length=470, Percent_Identity=28.5106382978723, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651554, Length=486, Percent_Identity=28.6008230452675, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24657835, Length=472, Percent_Identity=28.6016949152542, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24657842, Length=471, Percent_Identity=28.6624203821656, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24657827, Length=468, Percent_Identity=29.4871794871795, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24651556, Length=455, Percent_Identity=28.3516483516484, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47997; Mature: 47997

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFLLVFVLLLIVSLLVASGVKNVIYMIGDGMGINHVLLTSYLEGRELYMLQAPYTGFV
CHHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCEE
LTFSANSNVPDSASAGTALASGYKTNNGMIGMLPDGTVITNIAEIAAQYGIKTGIVATCR
EEEECCCCCCCCCCCCCHHHCCEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEE
ITHATPAAFYGHVSSRRDENTLAEQLVDSSLTVAFGGGWRHFVPTGGKRKDGKDLIAIAK
EECCCCHHHHHCHHCCCCHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCCEEEEEE
ANGFDYITTKTELLNYDGDRVLGLFSYSHLKAASERTGEEPMLPEMVEKALEILSKDDSP
CCCCEEEEEHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
FFLMVEGSQIDWEAHGNDVYGLWKEIVEFDNAVKVALDFAKEHPNTLIVITADHETGGLG
EEEEEECCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCCCCE
LSTGGYTMNLEKIRKDYTKTADWIVSNFGDSDEELKEAVKTYFNVELTDEDLAYYHEEKA
EECCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHH
KSFNGAGNAIGRILSNKAQIGWTTHDHTGGAVPIFAFGPGAELFSGVHDNTEIPNLIAYL
HCCCCCHHHHHHHHCCCCEECCEECCCCCCEEEEEEECCCHHHHCCCCCCCCHHHHHHHH
LGIGKVSDVEYGIEDVPLVGGH
HCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRFLLVFVLLLIVSLLVASGVKNVIYMIGDGMGINHVLLTSYLEGRELYMLQAPYTGFV
CHHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCEE
LTFSANSNVPDSASAGTALASGYKTNNGMIGMLPDGTVITNIAEIAAQYGIKTGIVATCR
EEEECCCCCCCCCCCCCHHHCCEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEE
ITHATPAAFYGHVSSRRDENTLAEQLVDSSLTVAFGGGWRHFVPTGGKRKDGKDLIAIAK
EECCCCHHHHHCHHCCCCHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCCEEEEEE
ANGFDYITTKTELLNYDGDRVLGLFSYSHLKAASERTGEEPMLPEMVEKALEILSKDDSP
CCCCEEEEEHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
FFLMVEGSQIDWEAHGNDVYGLWKEIVEFDNAVKVALDFAKEHPNTLIVITADHETGGLG
EEEEEECCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCCCCE
LSTGGYTMNLEKIRKDYTKTADWIVSNFGDSDEELKEAVKTYFNVELTDEDLAYYHEEKA
EECCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHH
KSFNGAGNAIGRILSNKAQIGWTTHDHTGGAVPIFAFGPGAELFSGVHDNTEIPNLIAYL
HCCCCCHHHHHHHHCCCCEECCEECCCCCCEEEEEEECCCHHHHCCCCCCCCHHHHHHHH
LGIGKVSDVEYGIEDVPLVGGH
HCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]