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The map label for this gene is ribH

Identifier: 239617613

GI number: 239617613

Start: 1311492

End: 1311962

Strand: Direct

Name: ribH

Synonym: Kole_1234

Alternate gene names: 239617613

Gene position: 1311492-1311962 (Clockwise)

Preceding gene: 239617612

Following gene: 239617614

Centisome position: 56.97

GC content: 45.44

Gene sequence:

>471_bases
ATGAGGGTTTTCGAAGGAAGATATTACGGAGAAGGGCTGAAGATCGCCATCGTGATTTCGAGATTTAATTCCGCTGTTAC
GAAGGAACTTTTAGACGGAGCTCTGGATGCTTTGAAGAGACACGAAGTAAAAGATGAAAACATAGATGTTATTTGGGTTC
CTGGTGCTATGGAAATACCGCACATCACAAGGTCAATCGCTCTCAAAAAGAAACACGACGCGATAATTGCGCTTGGCGCG
GTTATCCGTGGCGAGACATATCATTTCGACGTCGTGGCCAATGAAGTCTCGAAAGGTATCGCGGCGATCAACCTGGAAGT
GGATGTACCTGTATCGTTTGGAATCATCACATCCGACACTGTCGAGCAAGCCTTGAACAGAGCAGGCATTAAATCAGGAA
ACAAAGGTTTTGAAGCGGCTATGGTGGCGCTGGAAATGGCAAACCTAAAAAGGCAGATAGAAGCAATTTAA

Upstream 100 bases:

>100_bases
AAACAAGGAACATCTTCGGCGAGATAACACCATACAACCACTTCTATCTAAAGACAAAAATGGAAAGAATGGGTCACACA
TTTGAGGAGGTGCTTAAAAA

Downstream 100 bases:

>100_bases
TAAATTGTGGTTCAAGAGTTTGAAAGTGAAACAGTAAAAAACAAAGAAGCGTGGTACTGATGAATAATATGACTCTGTTG
ATACTCAAGAAATAGTCTTA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRVFEGRYYGEGLKIAIVISRFNSAVTKELLDGALDALKRHEVKDENIDVIWVPGAMEIPHITRSIALKKKHDAIIALGA
VIRGETYHFDVVANEVSKGIAAINLEVDVPVSFGIITSDTVEQALNRAGIKSGNKGFEAAMVALEMANLKRQIEAI

Sequences:

>Translated_156_residues
MRVFEGRYYGEGLKIAIVISRFNSAVTKELLDGALDALKRHEVKDENIDVIWVPGAMEIPHITRSIALKKKHDAIIALGA
VIRGETYHFDVVANEVSKGIAAINLEVDVPVSFGIITSDTVEQALNRAGIKSGNKGFEAAMVALEMANLKRQIEAI
>Mature_156_residues
MRVFEGRYYGEGLKIAIVISRFNSAVTKELLDGALDALKRHEVKDENIDVIWVPGAMEIPHITRSIALKKKHDAIIALGA
VIRGETYHFDVVANEVSKGIAAINLEVDVPVSFGIITSDTVEQALNRAGIKSGNKGFEAAMVALEMANLKRQIEAI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=49.3589743589744, Blast_Score=157, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=32.8859060402685, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_KOSOT (C5CJ15)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940935.1
- GeneID:   7969194
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1234
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17027; Mature: 17027

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFEGRYYGEGLKIAIVISRFNSAVTKELLDGALDALKRHEVKDENIDVIWVPGAMEIP
CCEECCEEECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
HITRSIALKKKHDAIIALGAVIRGETYHFDVVANEVSKGIAAINLEVDVPVSFGIITSDT
HHHHHHHHCCCCCEEEEEEHHHCCCEEEEEEEHHHHHCCEEEEEEEEECCEEECEEEHHH
VEQALNRAGIKSGNKGFEAAMVALEMANLKRQIEAI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVFEGRYYGEGLKIAIVISRFNSAVTKELLDGALDALKRHEVKDENIDVIWVPGAMEIP
CCEECCEEECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
HITRSIALKKKHDAIIALGAVIRGETYHFDVVANEVSKGIAAINLEVDVPVSFGIITSDT
HHHHHHHHCCCCCEEEEEEHHHCCCEEEEEEEHHHHHCCEEEEEEEEECCEEECEEEHHH
VEQALNRAGIKSGNKGFEAAMVALEMANLKRQIEAI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA