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The map label for this gene is degA [H]

Identifier: 239617602

GI number: 239617602

Start: 1299197

End: 1300198

Strand: Direct

Name: degA [H]

Synonym: Kole_1223

Alternate gene names: 239617602

Gene position: 1299197-1300198 (Clockwise)

Preceding gene: 239617596

Following gene: 239617603

Centisome position: 56.43

GC content: 38.92

Gene sequence:

>1002_bases
ATGAAAAAAGTTACCATAACAGATATTGCGAAACTTACTGGCTATTCTATAAAAACCGTTTCGCGGGTAATTAACAACGC
CCCTAATGTCAAAGAAGAAACACGTAGAAAAATTTTACGCGTTATCGAGGAGCATAATTATAGTGTAAACCTTCTCGCGA
AAGCCCTAAGAAACAGCGAGACCAGAACGGTGATCCTTTTTATCGATAGAAGAATGGGTGAGTACTGGAATGAATGGCAT
GACCTCATGTTAAAAAGTCTCATGGTTAAGTTTAGAAATAGAGGTTACAAAGTTATTCTCTCTCCATCCTCCGGTGAAGA
GACGATAAGTGATGACACCGATGGTTTCCATTTACTTAAAAGTGGGCTTGCCGATGGCGCTATCATGTTTGATGTAATAT
CGGAAGATTGCAGAGTTAGATATTTAAGAGCGAACAATATTCCCTTTGTAATTATCGGAAAGGACAAGGATTTTTCCGAT
ACAACTTTTGTAGACCTTGACAACTATCTTGTGGGGTATATTGGAGCGGAATACCTTATAAACAAAGGTTATGAAAATAT
AGCTCTATTTCTGGGGCCAAAAGTTTTTAACGTCAATCGCGAGAGGGCTCAGGGATTCGAAGAAGCCTGTGAAAAGCATG
GAATAGCTCATAGCGTTTTTTATGGGTTATCCACTATAAAAAGAGTCTATGAAAAAACCCACGAACTACTGGACAAAAGT
ATCGTGGATGCATTCTTTATCTCTGGAGATGCCAAAGTACTTGGAGCTTATAGAGCCATTCGAGAAAAAGGATTAAAGAT
TCCGGAAGATGTTGCTGTTCTTGGAATAGACAATCTGCCCTCTAGTGAATACATGTACCCGCCTTTAACTTCTATCGACC
AAAATGTCGAAAAATTCTCTGACCAGGCAGTTAATCTTTTGATAGAGATGATGCATTCAAAGAAGAAATCACCAAAGAGG
ATAATCCTGCCGCCCAGAATAATTGAAAGGCAATCAGTATGA

Upstream 100 bases:

>100_bases
ATTACTATTATTTGAATGTGGCGAAATTTCATACTTTTTTTAATTTATACTATAATAATGTGATCGACGATGATAATGTT
GTCATAAAAGGGGGAGTCTT

Downstream 100 bases:

>100_bases
AAGGATGTTGTATGTGAAGTTGTTAAAGGATGTAAATCACAATGGAACCAGATTGGATTATTCAGACTACTTAAAATATC
TTGAAGGTGAAAAAAATTCT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKVTITDIAKLTGYSIKTVSRVINNAPNVKEETRRKILRVIEEHNYSVNLLAKALRNSETRTVILFIDRRMGEYWNEWH
DLMLKSLMVKFRNRGYKVILSPSSGEETISDDTDGFHLLKSGLADGAIMFDVISEDCRVRYLRANNIPFVIIGKDKDFSD
TTFVDLDNYLVGYIGAEYLINKGYENIALFLGPKVFNVNRERAQGFEEACEKHGIAHSVFYGLSTIKRVYEKTHELLDKS
IVDAFFISGDAKVLGAYRAIREKGLKIPEDVAVLGIDNLPSSEYMYPPLTSIDQNVEKFSDQAVNLLIEMMHSKKKSPKR
IILPPRIIERQSV

Sequences:

>Translated_333_residues
MKKVTITDIAKLTGYSIKTVSRVINNAPNVKEETRRKILRVIEEHNYSVNLLAKALRNSETRTVILFIDRRMGEYWNEWH
DLMLKSLMVKFRNRGYKVILSPSSGEETISDDTDGFHLLKSGLADGAIMFDVISEDCRVRYLRANNIPFVIIGKDKDFSD
TTFVDLDNYLVGYIGAEYLINKGYENIALFLGPKVFNVNRERAQGFEEACEKHGIAHSVFYGLSTIKRVYEKTHELLDKS
IVDAFFISGDAKVLGAYRAIREKGLKIPEDVAVLGIDNLPSSEYMYPPLTSIDQNVEKFSDQAVNLLIEMMHSKKKSPKR
IILPPRIIERQSV
>Mature_333_residues
MKKVTITDIAKLTGYSIKTVSRVINNAPNVKEETRRKILRVIEEHNYSVNLLAKALRNSETRTVILFIDRRMGEYWNEWH
DLMLKSLMVKFRNRGYKVILSPSSGEETISDDTDGFHLLKSGLADGAIMFDVISEDCRVRYLRANNIPFVIIGKDKDFSD
TTFVDLDNYLVGYIGAEYLINKGYENIALFLGPKVFNVNRERAQGFEEACEKHGIAHSVFYGLSTIKRVYEKTHELLDKS
IVDAFFISGDAKVLGAYRAIREKGLKIPEDVAVLGIDNLPSSEYMYPPLTSIDQNVEKFSDQAVNLLIEMMHSKKKSPKR
IILPPRIIERQSV

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=29.1666666666667, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=30.1538461538462, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=26.5671641791045, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790369, Length=324, Percent_Identity=25.6172839506173, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=25.8160237388724, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=26.2536873156342, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=24.0853658536585, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=26.1764705882353, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789202, Length=351, Percent_Identity=23.0769230769231, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790715, Length=303, Percent_Identity=23.7623762376238, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=22.3564954682779, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786268, Length=329, Percent_Identity=24.3161094224924, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38005; Mature: 38005

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTITDIAKLTGYSIKTVSRVINNAPNVKEETRRKILRVIEEHNYSVNLLAKALRNSE
CCCEEHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TRTVILFIDRRMGEYWNEWHDLMLKSLMVKFRNRGYKVILSPSSGEETISDDTDGFHLLK
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHH
SGLADGAIMFDVISEDCRVRYLRANNIPFVIIGKDKDFSDTTFVDLDNYLVGYIGAEYLI
HCCCCCHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
NKGYENIALFLGPKVFNVNRERAQGFEEACEKHGIAHSVFYGLSTIKRVYEKTHELLDKS
HCCCCCEEEEECCEEECCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IVDAFFISGDAKVLGAYRAIREKGLKIPEDVAVLGIDNLPSSEYMYPPLTSIDQNVEKFS
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
DQAVNLLIEMMHSKKKSPKRIILPPRIIERQSV
HHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCC
>Mature Secondary Structure
MKKVTITDIAKLTGYSIKTVSRVINNAPNVKEETRRKILRVIEEHNYSVNLLAKALRNSE
CCCEEHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
TRTVILFIDRRMGEYWNEWHDLMLKSLMVKFRNRGYKVILSPSSGEETISDDTDGFHLLK
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHH
SGLADGAIMFDVISEDCRVRYLRANNIPFVIIGKDKDFSDTTFVDLDNYLVGYIGAEYLI
HCCCCCHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
NKGYENIALFLGPKVFNVNRERAQGFEEACEKHGIAHSVFYGLSTIKRVYEKTHELLDKS
HCCCCCEEEEECCEEECCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IVDAFFISGDAKVLGAYRAIREKGLKIPEDVAVLGIDNLPSSEYMYPPLTSIDQNVEKFS
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
DQAVNLLIEMMHSKKKSPKRIILPPRIIERQSV
HHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]