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The map label for this gene is pqqE [H]

Identifier: 239617437

GI number: 239617437

Start: 1119082

End: 1120491

Strand: Reverse

Name: pqqE [H]

Synonym: Kole_1050

Alternate gene names: 239617437

Gene position: 1120491-1119082 (Counterclockwise)

Preceding gene: 239617438

Following gene: 239617436

Centisome position: 48.67

GC content: 35.32

Gene sequence:

>1410_bases
ATGCTCAACAGCTTGCAAATTTCAAGTATAAGATGGGAAATTGAACCAAAATGTAATCTCAATTGCAAGCACTGCTTTGT
AGGGAGTAAATTAGATAGTTTCCAGGAGCTTGATCTTGACAGTGCAATGAAAATAGTGAATAAGATAGCAAGTATTGGGG
TTAAAAAAGTAAATTTCACAACGAAAGAACCAACATTGTTTAAAGAACTTGACAAGCTTATCTTTTACTGTTCATTGAAA
GGGATTTATACTGAACTTGTTACAAATGGTGTATTACTTCGGGATATTACACTATCAGAGAAAATTGTAAATAGCGGTGT
TGGTGCAATAAATATAAGCATGGAAGGTATTAGCGATACATCGAACGATTATATACGTGGTAAAGGAACGTTTGTTCAGA
TTTTAAAGGCGCTAAATAATCTGAAAAATGTTATGGAACCAGGAAGGTACGTGCCCATTGTTATTCAAATGACTTTGAAT
AGGAAGAACAAATTAGAAGTTCATAAGATACCTGCATTTTTCAATTCACTTCCAGTTGATATGGTGGTTATAGGTGGGCT
TTTATTGGAGGGAAATGCTCGGGAGAACCCAGATATAGCTTTATCATCTGAAGAATTTTTAGAAGCGTGGGAAATTATTT
TGGAAGAGTACTTATCCCTAGACTATAAGAGATATTATCTCAATTCCAAATCATTGTTTCCTCCTGAAGCAGTTTACTAC
AATATCATGTATGGTACGGATTTATGTCCATTGCCACCAAGGTGTGGTGTATTAAACGGGAATTTTTCGCTACTTCCAAA
CGGCTGTATTATTCCATGTGTCGCTATTTTGGATAAAGAGGCAAATGTTGGCAGGCTCCCAATGATCGATCTCTTAAACA
AAGAGTTTATTGAAGAAAAAGACAAAAAACGAATGGATGATTTTATTAACAGTGTTAGTGAATTAATAAAACTGCGGAAA
CCTTATATTTGTTCTCAATGCTACTATGAAGATTGGTGTATGCCTTGCCCAGTTAGTTTGCTCCAAAAGGGGTTTTTAGA
TGAAATTTCCGAAAGATGTGAGCGTGCCTTGAAAGAGCTTGAAATGTTTTTACTAAATATCCAAAAAGCTTTCAAAGAAT
ACTATGTTGTGGTTAGTCAAGATACGCTGATGCACTTGGAAAAAGATAAAGCTTTCTTGACAAGGTTTTATAGATTAGGT
GAAACGCTGCAAAGAAAGTACAAAGTTGATGGATACAAAGCAAGGATACTGAAAAGTATTTTTGATAACGGAGCAATTGC
TCTTCCAGATTTATGTTCAGATCTATCTGTGACCTCTGCGGACGTCTTGAACTTTCTTAAACCATTATTTTACGACGGAT
TTATTGAATTCAGGAGGTCTGAAAGAGTCCCTTTTTACGCTTCAAAGTGA

Upstream 100 bases:

>100_bases
TGTTAAGGAAATCGGGCTTTACCAAAGAAATTGTATTAGAAGGCGAATTTTATACAGCTGATCTGGTTGTATATTCATAC
TTTTTGGGGTGAGTGATACC

Downstream 100 bases:

>100_bases
TGAGCATTTAAACATTCATCGTGATCGTGATTGCTTGACAAAAAAGATGAAGAAAAGATGGGGGTAATGCATTGCGATAA
GAATACAAGGCACTTTGAGC

Product: Radical SAM domain protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLNSLQISSIRWEIEPKCNLNCKHCFVGSKLDSFQELDLDSAMKIVNKIASIGVKKVNFTTKEPTLFKELDKLIFYCSLK
GIYTELVTNGVLLRDITLSEKIVNSGVGAINISMEGISDTSNDYIRGKGTFVQILKALNNLKNVMEPGRYVPIVIQMTLN
RKNKLEVHKIPAFFNSLPVDMVVIGGLLLEGNARENPDIALSSEEFLEAWEIILEEYLSLDYKRYYLNSKSLFPPEAVYY
NIMYGTDLCPLPPRCGVLNGNFSLLPNGCIIPCVAILDKEANVGRLPMIDLLNKEFIEEKDKKRMDDFINSVSELIKLRK
PYICSQCYYEDWCMPCPVSLLQKGFLDEISERCERALKELEMFLLNIQKAFKEYYVVVSQDTLMHLEKDKAFLTRFYRLG
ETLQRKYKVDGYKARILKSIFDNGAIALPDLCSDLSVTSADVLNFLKPLFYDGFIEFRRSERVPFYASK

Sequences:

>Translated_469_residues
MLNSLQISSIRWEIEPKCNLNCKHCFVGSKLDSFQELDLDSAMKIVNKIASIGVKKVNFTTKEPTLFKELDKLIFYCSLK
GIYTELVTNGVLLRDITLSEKIVNSGVGAINISMEGISDTSNDYIRGKGTFVQILKALNNLKNVMEPGRYVPIVIQMTLN
RKNKLEVHKIPAFFNSLPVDMVVIGGLLLEGNARENPDIALSSEEFLEAWEIILEEYLSLDYKRYYLNSKSLFPPEAVYY
NIMYGTDLCPLPPRCGVLNGNFSLLPNGCIIPCVAILDKEANVGRLPMIDLLNKEFIEEKDKKRMDDFINSVSELIKLRK
PYICSQCYYEDWCMPCPVSLLQKGFLDEISERCERALKELEMFLLNIQKAFKEYYVVVSQDTLMHLEKDKAFLTRFYRLG
ETLQRKYKVDGYKARILKSIFDNGAIALPDLCSDLSVTSADVLNFLKPLFYDGFIEFRRSERVPFYASK
>Mature_469_residues
MLNSLQISSIRWEIEPKCNLNCKHCFVGSKLDSFQELDLDSAMKIVNKIASIGVKKVNFTTKEPTLFKELDKLIFYCSLK
GIYTELVTNGVLLRDITLSEKIVNSGVGAINISMEGISDTSNDYIRGKGTFVQILKALNNLKNVMEPGRYVPIVIQMTLN
RKNKLEVHKIPAFFNSLPVDMVVIGGLLLEGNARENPDIALSSEEFLEAWEIILEEYLSLDYKRYYLNSKSLFPPEAVYY
NIMYGTDLCPLPPRCGVLNGNFSLLPNGCIIPCVAILDKEANVGRLPMIDLLNKEFIEEKDKKRMDDFINSVSELIKLRK
PYICSQCYYEDWCMPCPVSLLQKGFLDEISERCERALKELEMFLLNIQKAFKEYYVVVSQDTLMHLEKDKAFLTRFYRLG
ETLQRKYKVDGYKARILKSIFDNGAIALPDLCSDLSVTSADVLNFLKPLFYDGFIEFRRSERVPFYASK

Specific function: Unknown

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53703; Mature: 53703

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSLQISSIRWEIEPKCNLNCKHCFVGSKLDSFQELDLDSAMKIVNKIASIGVKKVNFT
CCCCEEEEEEEEEECCCCCCCCCEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEECC
TKEPTLFKELDKLIFYCSLKGIYTELVTNGVLLRDITLSEKIVNSGVGAINISMEGISDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCEEEEEECCCCCC
SNDYIRGKGTFVQILKALNNLKNVMEPGRYVPIVIQMTLNRKNKLEVHKIPAFFNSLPVD
CCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEHHHHHCCCCHH
MVVIGGLLLEGNARENPDIALSSEEFLEAWEIILEEYLSLDYKRYYLNSKSLFPPEAVYY
HHHHCCEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCCHHEEE
NIMYGTDLCPLPPRCGVLNGNFSLLPNGCIIPCVAILDKEANVGRLPMIDLLNKEFIEEK
EEEECCCCCCCCCCCCEECCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
DKKRMDDFINSVSELIKLRKPYICSQCYYEDWCMPCPVSLLQKGFLDEISERCERALKEL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EMFLLNIQKAFKEYYVVVSQDTLMHLEKDKAFLTRFYRLGETLQRKYKVDGYKARILKSI
HHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FDNGAIALPDLCSDLSVTSADVLNFLKPLFYDGFIEFRRSERVPFYASK
HCCCCEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MLNSLQISSIRWEIEPKCNLNCKHCFVGSKLDSFQELDLDSAMKIVNKIASIGVKKVNFT
CCCCEEEEEEEEEECCCCCCCCCEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEECC
TKEPTLFKELDKLIFYCSLKGIYTELVTNGVLLRDITLSEKIVNSGVGAINISMEGISDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCEEEEEECCCCCC
SNDYIRGKGTFVQILKALNNLKNVMEPGRYVPIVIQMTLNRKNKLEVHKIPAFFNSLPVD
CCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEHHHHHCCCCHH
MVVIGGLLLEGNARENPDIALSSEEFLEAWEIILEEYLSLDYKRYYLNSKSLFPPEAVYY
HHHHCCEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCCHHEEE
NIMYGTDLCPLPPRCGVLNGNFSLLPNGCIIPCVAILDKEANVGRLPMIDLLNKEFIEEK
EEEECCCCCCCCCCCCEECCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
DKKRMDDFINSVSELIKLRKPYICSQCYYEDWCMPCPVSLLQKGFLDEISERCERALKEL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EMFLLNIQKAFKEYYVVVSQDTLMHLEKDKAFLTRFYRLGETLQRKYKVDGYKARILKSI
HHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FDNGAIALPDLCSDLSVTSADVLNFLKPLFYDGFIEFRRSERVPFYASK
HCCCCEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]