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The map label for this gene is regA [H]

Identifier: 239617428

GI number: 239617428

Start: 1106899

End: 1107909

Strand: Reverse

Name: regA [H]

Synonym: Kole_1040

Alternate gene names: 239617428

Gene position: 1107909-1106899 (Counterclockwise)

Preceding gene: 239617429

Following gene: 239617427

Centisome position: 48.13

GC content: 41.25

Gene sequence:

>1011_bases
ATGGTGAAAACGGTTCAACCAACAATCGAAATGGTTGCCAAAGCCGCGGGAGTTTCAAAGATGACTGTATCCCGGGCAAT
AAACCACCCTGAAAAGGTTAACCCCGAAACATTAAACCTGATCATGGAAACCATGAAAAAACTTAATTACCGCCCGAGAT
TTGTGGCAAGAGTGCTTGCAGGGGGGAAATCCAGAACAATTGGTTTTCTCGTAAAATCAAATGAAGACTTCATCATCTCA
CCGTTTTATGGAGAATGTATAAGAGCAGCTGTCGAATGGCTCAAGACTCAGAACTACCGTAGTGTTATTTTCAATCTGCT
TGACTCTCAAGGTAAATCCCTCTTTGTGGACTATATGAACTCCGGACTTCTTGATGGTCTCATCGTTTTCGAGGGCATTT
ACGAACGTGAACTACTGGAAACGCTAAAGATAAATTCCATACCTTCCATCCTCGTTGGAGAGAATCTCATGAAAGAGTAC
GATTTCGTCACGGTATCTTCAGACAATTACGGTGGAGCAAATAAAGCCGTTGAACATCTAATAAAAACTGGCTGTTCGAG
AATAGTCTATGTAACCGGCATGGGGGAAAAACCTTCATACCTCCACAGGAAAAAGGCTTATATAGATACCCTCAAAAAAT
ACAAAATAAGCTATTGCAAGATAGTCGAGACTCCAGTGAGTATAAATGGTGGAAAAAAAGCGGTAGAAAAACTTCTCAAA
GAAGGCGAAGACTTTGATGGGCTGTTCTGCTTTTCAGATTTAATAGCTATTGGTGCGCTAAGAGCGCTCTACGAAAACCG
GGTAAAAGTCCCGAGTGAGGTAAAAGTAGTTGGGTTCGACAATATACGCCTTTCGAGAGATTTTGTACCTTCTTTAACTA
CTGTTGCGCAGAATATGCACCTTATGGGAGAACTCGCATCAAATATGCTCTTAAGAATTCTATCAGGAAAAAAGGCAAAA
CAGAAAAATATACTTCTATCCACAGAACTCATCGTTCGGGAAAGCGCTTGA

Upstream 100 bases:

>100_bases
CCACCATACTTCATTTGTGACATGAGTTAATCCTTGCTAAATAACTTTGGAAAATCTATTGTATTATGAGATAATGTTAC
CGGTAACATGAGGAGGGTGG

Downstream 100 bases:

>100_bases
ATGTCACGGATGGTGTTTGCCAGTGAGCTTTTCTGTCTGTATTTTTGATATGGATGGAGTAATTGTTGATACTGCTAGGT
ATCACTTTCTAGCATGGAAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MVKTVQPTIEMVAKAAGVSKMTVSRAINHPEKVNPETLNLIMETMKKLNYRPRFVARVLAGGKSRTIGFLVKSNEDFIIS
PFYGECIRAAVEWLKTQNYRSVIFNLLDSQGKSLFVDYMNSGLLDGLIVFEGIYERELLETLKINSIPSILVGENLMKEY
DFVTVSSDNYGGANKAVEHLIKTGCSRIVYVTGMGEKPSYLHRKKAYIDTLKKYKISYCKIVETPVSINGGKKAVEKLLK
EGEDFDGLFCFSDLIAIGALRALYENRVKVPSEVKVVGFDNIRLSRDFVPSLTTVAQNMHLMGELASNMLLRILSGKKAK
QKNILLSTELIVRESA

Sequences:

>Translated_336_residues
MVKTVQPTIEMVAKAAGVSKMTVSRAINHPEKVNPETLNLIMETMKKLNYRPRFVARVLAGGKSRTIGFLVKSNEDFIIS
PFYGECIRAAVEWLKTQNYRSVIFNLLDSQGKSLFVDYMNSGLLDGLIVFEGIYERELLETLKINSIPSILVGENLMKEY
DFVTVSSDNYGGANKAVEHLIKTGCSRIVYVTGMGEKPSYLHRKKAYIDTLKKYKISYCKIVETPVSINGGKKAVEKLLK
EGEDFDGLFCFSDLIAIGALRALYENRVKVPSEVKVVGFDNIRLSRDFVPSLTTVAQNMHLMGELASNMLLRILSGKKAK
QKNILLSTELIVRESA
>Mature_336_residues
MVKTVQPTIEMVAKAAGVSKMTVSRAINHPEKVNPETLNLIMETMKKLNYRPRFVARVLAGGKSRTIGFLVKSNEDFIIS
PFYGECIRAAVEWLKTQNYRSVIFNLLDSQGKSLFVDYMNSGLLDGLIVFEGIYERELLETLKINSIPSILVGENLMKEY
DFVTVSSDNYGGANKAVEHLIKTGCSRIVYVTGMGEKPSYLHRKKAYIDTLKKYKISYCKIVETPVSINGGKKAVEKLLK
EGEDFDGLFCFSDLIAIGALRALYENRVKVPSEVKVVGFDNIRLSRDFVPSLTTVAQNMHLMGELASNMLLRILSGKKAK
QKNILLSTELIVRESA

Specific function: Involved in the regulation of amylase production [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=29.2397660818713, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=30.6306306306306, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=27.4626865671642, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=28.3582089552239, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=29.1095890410959, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=28.2958199356913, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789068, Length=295, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=26.5060240963855, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=24.223602484472, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=23.4939759036145, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787906, Length=341, Percent_Identity=24.633431085044, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37596; Mature: 37596

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKTVQPTIEMVAKAAGVSKMTVSRAINHPEKVNPETLNLIMETMKKLNYRPRFVARVLA
CCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
GGKSRTIGFLVKSNEDFIISPFYGECIRAAVEWLKTQNYRSVIFNLLDSQGKSLFVDYMN
CCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEHHHHH
SGLLDGLIVFEGIYERELLETLKINSIPSILVGENLMKEYDFVTVSSDNYGGANKAVEHL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHH
IKTGCSRIVYVTGMGEKPSYLHRKKAYIDTLKKYKISYCKIVETPVSINGGKKAVEKLLK
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
EGEDFDGLFCFSDLIAIGALRALYENRVKVPSEVKVVGFDNIRLSRDFVPSLTTVAQNMH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEECHHHHHHHHHHHHHHH
LMGELASNMLLRILSGKKAKQKNILLSTELIVRESA
HHHHHHHHHHHHHHCCCCCHHCCEEEEHHEEEECCC
>Mature Secondary Structure
MVKTVQPTIEMVAKAAGVSKMTVSRAINHPEKVNPETLNLIMETMKKLNYRPRFVARVLA
CCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
GGKSRTIGFLVKSNEDFIISPFYGECIRAAVEWLKTQNYRSVIFNLLDSQGKSLFVDYMN
CCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEHHHHH
SGLLDGLIVFEGIYERELLETLKINSIPSILVGENLMKEYDFVTVSSDNYGGANKAVEHL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHH
IKTGCSRIVYVTGMGEKPSYLHRKKAYIDTLKKYKISYCKIVETPVSINGGKKAVEKLLK
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
EGEDFDGLFCFSDLIAIGALRALYENRVKVPSEVKVVGFDNIRLSRDFVPSLTTVAQNMH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEECHHHHHHHHHHHHHHH
LMGELASNMLLRILSGKKAKQKNILLSTELIVRESA
HHHHHHHHHHHHHHCCCCCHHCCEEEEHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]