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The map label for this gene is pcm [H]

Identifier: 239617391

GI number: 239617391

Start: 1058071

End: 1058805

Strand: Reverse

Name: pcm [H]

Synonym: Kole_1001

Alternate gene names: 239617391

Gene position: 1058805-1058071 (Counterclockwise)

Preceding gene: 239617392

Following gene: 239617387

Centisome position: 45.99

GC content: 41.5

Gene sequence:

>735_bases
ATGAAGTCCCGTGACCTCGCCGATGAACTGCTCCAGAGGAACATCATAAGAGATTCAAGAATTTATCAGGCGATGTGCGA
AATTGACAGAAGCGAGTTTGTCCTGCCGAAATATAAATTCCTCGCGCACATCGATGAAGTCCTTCCTTCCGCAGAAAAGA
ACGGGTTCATCATCAGCACCTCAACCCAACCATCCCTTGTTGTCCAAATGATTCAAAATCTAAATCTCACAGGAAATGAG
AAAGTGCTTGACATAGGGACAGGAACAGGATATGCCACTCTAATACTCGCCAAAATTCTCAAAGATGGCGAAGTCATTAG
CATCGAGTACAATCGTGAACTCCATGAAATAGCACTAAGGAATTTCGAACGTTATGGGGTTGAAAATATTACCTGCATCA
GTGGAGACGGATTTGAGGGATATAGTTTGAAGGCTCCTTACGATGCCATAATCTCTATGGTTGCTTGCGAAACCCTTCCC
GTTCAATGGATAGAACAACTCAAAAGTTCAGGTGGAAGGATCGTTGCCCCGATTGTCTTCAACAGCTTTTACACACCAGT
TTATAAGTTCACAAGAAAGGGAGAGCAAATACACGCTTATAAGTTAATAGACGCTGTTTTTATGTCAATGAAAGGGCAAC
ACGAATATAGAACGTTCTTACCTCAGATCTTCGACTACGAACTTATTTTCGATGGAAAAAATTTAAAACCCATTGATGAA
AACACTCTGGATTAA

Upstream 100 bases:

>100_bases
TAATACGTGAAAGAAGACAAAAAAAAACAAAAATTGGCTAAAAATCCGAAGAAGGGAGGTACTGGCATTTCATTAATAAT
ACGAGGAATGCCTAAACATC

Downstream 100 bases:

>100_bases
GAAACACCAAACAGTGCTCATAAAAAAGCCGGGAGATGACCTCCCGGCTTGATTCTTTTTATTCAAACTCAGATATTGTG
CTTTTTCAGGAACTCATCTA

Product: Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKSRDLADELLQRNIIRDSRIYQAMCEIDRSEFVLPKYKFLAHIDEVLPSAEKNGFIISTSTQPSLVVQMIQNLNLTGNE
KVLDIGTGTGYATLILAKILKDGEVISIEYNRELHEIALRNFERYGVENITCISGDGFEGYSLKAPYDAIISMVACETLP
VQWIEQLKSSGGRIVAPIVFNSFYTPVYKFTRKGEQIHAYKLIDAVFMSMKGQHEYRTFLPQIFDYELIFDGKNLKPIDE
NTLD

Sequences:

>Translated_244_residues
MKSRDLADELLQRNIIRDSRIYQAMCEIDRSEFVLPKYKFLAHIDEVLPSAEKNGFIISTSTQPSLVVQMIQNLNLTGNE
KVLDIGTGTGYATLILAKILKDGEVISIEYNRELHEIALRNFERYGVENITCISGDGFEGYSLKAPYDAIISMVACETLP
VQWIEQLKSSGGRIVAPIVFNSFYTPVYKFTRKGEQIHAYKLIDAVFMSMKGQHEYRTFLPQIFDYELIFDGKNLKPIDE
NTLD
>Mature_244_residues
MKSRDLADELLQRNIIRDSRIYQAMCEIDRSEFVLPKYKFLAHIDEVLPSAEKNGFIISTSTQPSLVVQMIQNLNLTGNE
KVLDIGTGTGYATLILAKILKDGEVISIEYNRELHEIALRNFERYGVENITCISGDGFEGYSLKAPYDAIISMVACETLP
VQWIEQLKSSGGRIVAPIVFNSFYTPVYKFTRKGEQIHAYKLIDAVFMSMKGQHEYRTFLPQIFDYELIFDGKNLKPIDE
NTLD

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=182, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789100, Length=179, Percent_Identity=31.8435754189944, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983477, Length=201, Percent_Identity=29.3532338308458, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=201, Percent_Identity=29.3532338308458, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17981723, Length=185, Percent_Identity=27.5675675675676, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 27822; Mature: 27822

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRDLADELLQRNIIRDSRIYQAMCEIDRSEFVLPKYKFLAHIDEVLPSAEKNGFIIST
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEC
STQPSLVVQMIQNLNLTGNEKVLDIGTGTGYATLILAKILKDGEVISIEYNRELHEIALR
CCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
NFERYGVENITCISGDGFEGYSLKAPYDAIISMVACETLPVQWIEQLKSSGGRIVAPIVF
HHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEHHHH
NSFYTPVYKFTRKGEQIHAYKLIDAVFMSMKGQHEYRTFLPQIFDYELIFDGKNLKPIDE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCC
NTLD
CCCC
>Mature Secondary Structure
MKSRDLADELLQRNIIRDSRIYQAMCEIDRSEFVLPKYKFLAHIDEVLPSAEKNGFIIST
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEC
STQPSLVVQMIQNLNLTGNEKVLDIGTGTGYATLILAKILKDGEVISIEYNRELHEIALR
CCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
NFERYGVENITCISGDGFEGYSLKAPYDAIISMVACETLPVQWIEQLKSSGGRIVAPIVF
HHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEHHHH
NSFYTPVYKFTRKGEQIHAYKLIDAVFMSMKGQHEYRTFLPQIFDYELIFDGKNLKPIDE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCC
NTLD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA