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The map label for this gene is mdh [H]

Identifier: 239617324

GI number: 239617324

Start: 987358

End: 988416

Strand: Reverse

Name: mdh [H]

Synonym: Kole_0933

Alternate gene names: 239617324

Gene position: 988416-987358 (Counterclockwise)

Preceding gene: 239617325

Following gene: 239617323

Centisome position: 42.93

GC content: 40.7

Gene sequence:

>1059_bases
ATGAAGGTAAAAAGAGAAAAGTTAATGAACTTTACCGCTGATGTGATGAAAAAGATGGGATGCGACGATGAAAAGGCGAG
AGTGGTTGCCGAAGTACTCGTTGAAGCAGATATGAGGGGAATATCGTCACACGGAGTAGCAAGGTTAAAACGATACGTAA
ATCATATAAAAGAAAGGATTATAGATCCGAATGGTGAACCGGAAATAATCTTTGAAACTCCTGTTACTTTAGCTATAGAC
GGAAACAACGGGGTAGGACAACACATTGCTAAATTTTCAATGGAAAAGGTCATAGAAAAAGCGAAAAAATACGGAATTGG
AATGGCAACCGTCCGAAATTCAAACCACTATGGAATAGCTGGATATTACGCTGAAATGGCTGTGAAAATGGGTTTAGTAG
GAATATCACTCACCAATACAGCCCCTCTGGTTGTTCATACATTCTCGAGGGAAGCCCTTCTTGGAACAAACCCAATAGCT
TTTGCTTTTCCAACAAGTGAAAAATATCCGATACTCATAGATATGGCAACAAGTGTTGCGCCCCGGGGAAAACTGGAAGT
GTATTCCAGGTTGAACAAAAAGATTCCGAAAGGCTGGGCCGTAGATGAAACGGGGCATACAACAACAGATCCAGAAAAAG
TTCTTTCTAATTTAAAACAGAGAAAAGGCGGAGGACTTCTGCCTTTAGGTGGAGAAGGGGAAGAATTTAGCGGTCATAAA
GGTTATGGATTGGTGCTTCTGGTTGAACTTCTGACTTCGGGGTTGTCGCTCGGTTCTTTTAGTTACGATACTTATAAAGG
AAAAGGTAAAATAACACACTTTTTTGCCGCACTTGACCCGGCGATTTTCGGCAATCCTGAGAAAATAAAGGAACATGCGA
TGGAGTTGATAAACAGTTTGAGAAGCGCTAAAAAAGCGGATGGAAAAGAAAGGATTTATGTGCACGGAGAAAAAGAATAT
GAAAAACGCGAGAAATCACTCGAATGCGGAGTGGAGATTGACGAACCTACATATTATGAATTGAGAAAAATCGCTGAAAA
TTTAGACATTCCCTGGTAG

Upstream 100 bases:

>100_bases
TGTGTGAATGTGACTACCCGATAAAACTGCAAGGGCTCAGAAAATGTGTTAAAGTCCTTTCAGGTAGCCAAAAAGCTGGT
TGGCCAGTGGGGTGGAAAAA

Downstream 100 bases:

>100_bases
AGAGGAGAGGAAAGATAAAGTGCTCATTTTTAAGCAAGTTCTTGAAGCCTTTGCAGTCGGAGACGCTATGGGGATGCCCA
CTGAATTCATGACCTTAAAA

Product: Malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKVKREKLMNFTADVMKKMGCDDEKARVVAEVLVEADMRGISSHGVARLKRYVNHIKERIIDPNGEPEIIFETPVTLAID
GNNGVGQHIAKFSMEKVIEKAKKYGIGMATVRNSNHYGIAGYYAEMAVKMGLVGISLTNTAPLVVHTFSREALLGTNPIA
FAFPTSEKYPILIDMATSVAPRGKLEVYSRLNKKIPKGWAVDETGHTTTDPEKVLSNLKQRKGGGLLPLGGEGEEFSGHK
GYGLVLLVELLTSGLSLGSFSYDTYKGKGKITHFFAALDPAIFGNPEKIKEHAMELINSLRSAKKADGKERIYVHGEKEY
EKREKSLECGVEIDEPTYYELRKIAENLDIPW

Sequences:

>Translated_352_residues
MKVKREKLMNFTADVMKKMGCDDEKARVVAEVLVEADMRGISSHGVARLKRYVNHIKERIIDPNGEPEIIFETPVTLAID
GNNGVGQHIAKFSMEKVIEKAKKYGIGMATVRNSNHYGIAGYYAEMAVKMGLVGISLTNTAPLVVHTFSREALLGTNPIA
FAFPTSEKYPILIDMATSVAPRGKLEVYSRLNKKIPKGWAVDETGHTTTDPEKVLSNLKQRKGGGLLPLGGEGEEFSGHK
GYGLVLLVELLTSGLSLGSFSYDTYKGKGKITHFFAALDPAIFGNPEKIKEHAMELINSLRSAKKADGKERIYVHGEKEY
EKREKSLECGVEIDEPTYYELRKIAENLDIPW
>Mature_352_residues
MKVKREKLMNFTADVMKKMGCDDEKARVVAEVLVEADMRGISSHGVARLKRYVNHIKERIIDPNGEPEIIFETPVTLAID
GNNGVGQHIAKFSMEKVIEKAKKYGIGMATVRNSNHYGIAGYYAEMAVKMGLVGISLTNTAPLVVHTFSREALLGTNPIA
FAFPTSEKYPILIDMATSVAPRGKLEVYSRLNKKIPKGWAVDETGHTTTDPEKVLSNLKQRKGGGLLPLGGEGEEFSGHK
GYGLVLLVELLTSGLSLGSFSYDTYKGKGKITHFFAALDPAIFGNPEKIKEHAMELINSLRSAKKADGKERIYVHGEKEY
EKREKSLECGVEIDEPTYYELRKIAENLDIPW

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786727, Length=350, Percent_Identity=33.1428571428571, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787020, Length=356, Percent_Identity=27.247191011236, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790000, Length=349, Percent_Identity=27.2206303724928, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17507179, Length=326, Percent_Identity=36.8098159509202, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17536633, Length=330, Percent_Identity=34.2424242424242, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI45553211, Length=316, Percent_Identity=36.7088607594937, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24667912, Length=316, Percent_Identity=36.7088607594937, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24667908, Length=316, Percent_Identity=36.7088607594937, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24667904, Length=316, Percent_Identity=36.7088607594937, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24658010, Length=312, Percent_Identity=30.7692307692308, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24648360, Length=350, Percent_Identity=26.2857142857143, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 38991; Mature: 38991

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKREKLMNFTADVMKKMGCDDEKARVVAEVLVEADMRGISSHGVARLKRYVNHIKERI
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IDPNGEPEIIFETPVTLAIDGNNGVGQHIAKFSMEKVIEKAKKYGIGMATVRNSNHYGIA
CCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHH
GYYAEMAVKMGLVGISLTNTAPLVVHTFSREALLGTNPIAFAFPTSEKYPILIDMATSVA
HHHHHHHHHHHHEEEEECCCCCEEEEECCCCCEECCCCEEEEECCCCCCCEEEEECCCCC
PRGKLEVYSRLNKKIPKGWAVDETGHTTTDPEKVLSNLKQRKGGGLLPLGGEGEEFSGHK
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
GYGLVLLVELLTSGLSLGSFSYDTYKGKGKITHFFAALDPAIFGNPEKIKEHAMELINSL
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHHHHH
RSAKKADGKERIYVHGEKEYEKREKSLECGVEIDEPTYYELRKIAENLDIPW
HHHHHCCCCCEEEEECCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVKREKLMNFTADVMKKMGCDDEKARVVAEVLVEADMRGISSHGVARLKRYVNHIKERI
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IDPNGEPEIIFETPVTLAIDGNNGVGQHIAKFSMEKVIEKAKKYGIGMATVRNSNHYGIA
CCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHH
GYYAEMAVKMGLVGISLTNTAPLVVHTFSREALLGTNPIAFAFPTSEKYPILIDMATSVA
HHHHHHHHHHHHEEEEECCCCCEEEEECCCCCEECCCCEEEEECCCCCCCEEEEECCCCC
PRGKLEVYSRLNKKIPKGWAVDETGHTTTDPEKVLSNLKQRKGGGLLPLGGEGEEFSGHK
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCC
GYGLVLLVELLTSGLSLGSFSYDTYKGKGKITHFFAALDPAIFGNPEKIKEHAMELINSL
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHHHHH
RSAKKADGKERIYVHGEKEYEKREKSLECGVEIDEPTYYELRKIAENLDIPW
HHHHHCCCCCEEEEECCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]