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The map label for this gene is ddlB [C]

Identifier: 239617317

GI number: 239617317

Start: 980753

End: 981718

Strand: Direct

Name: ddlB [C]

Synonym: Kole_0926

Alternate gene names: 239617317

Gene position: 980753-981718 (Clockwise)

Preceding gene: 239617316

Following gene: 239617334

Centisome position: 42.6

GC content: 40.17

Gene sequence:

>966_bases
ATGAAAGTCGCGGTTGTTTATGACCTTCCAATGAGTGAGGAAGATAAAAGGATGGTGGAAGCCGTTATCAGGGCTCTTTC
TAAGAAGCATGATTCTCAACCTCTTCCAATAGACGAAAAATTTATGGAAACAGTGAAAGAATACGACTTTGCTTTCAACC
TCTCAACCGGTGGTGGAAAACTTTCCAAACAGGTTCATGCTCCTGCGATTTTAGATAGGCTTGGAGTACCTTTCACCGGC
TCATCGGCGATCGTCCACGCAATATGCATTGATAAAAGCATAACAAAACTGATACTGGAAAAATCCGGTATCCCCACCCC
AAGGTTCGTCAAGATAGATGACCCGAATAATATTCCTGAAATAGACTTTTACCCAGCCTTTGTTAAACCCTCCTGCGAAG
GAAGTGCTCTTGGCATCTCCAGAGATTCCGTTGTAAAAAACCAGAAGGAATTGAAAAAACAGGTTGAGATGATCATGAAC
AAATTCAATCAACCTGCGATCGTAGAAGAGTTTATAGACGGAAGAGAATTTACCGTTGGAATTATAGGTAATGGTAATGA
TTTGAAAGTTTTACCCATACTGGAGATCGATTTTTCAAATCTTCCAGAAGGATTGGAACATTTTTATTCCCACAGGGTGA
AACATCATTATGGGGAAAAAACGACCTACATTTGTCCTGCAAGGTTGGAAGAAAGCCAGAGAAAGAAGTTAGAAAATTAC
GCAAAAAGAGCCTTTAAAGCTATAGGTCTCTTTGATTTTGCACGGATCGATGTTAGAATGCGAGTTAACGAATTTTATAT
AATCGAAGTTAATTCACTACCGCTTCTTGTTCCTGTTTATTCAGACCTGACGAAAATGCTTGCACCTATTGGCTGGAAGT
ACGAGGATCTTGTTAACGCAATTCTTGAGGCAGCGATTGCACGATACCAGAAGTCTTCTGAAAACTCAAAACTCAATCTC
GGATAA

Upstream 100 bases:

>100_bases
ATAAATGGTGCTTATTACGCTGTGAATGAAAAGGGAAAACCGATTTCCGAAGCTCAAAGACTTAAGTCGCTGGGATTTGA
AGTATGAAGGAGATGATAGG

Downstream 100 bases:

>100_bases
GGAAGATTCCACAGATTCAAGGAACGGCCATTGAGAAGCTCCTAACTCATTAACATCTTTCCAGTGTACCTGTGTCTTGA
TTTCTATTTTCCTGACATCC

Product: D-alanine--D-alanine ligase

Products: ADP; phosphate; D-alanyl-D-alanine

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKVAVVYDLPMSEEDKRMVEAVIRALSKKHDSQPLPIDEKFMETVKEYDFAFNLSTGGGKLSKQVHAPAILDRLGVPFTG
SSAIVHAICIDKSITKLILEKSGIPTPRFVKIDDPNNIPEIDFYPAFVKPSCEGSALGISRDSVVKNQKELKKQVEMIMN
KFNQPAIVEEFIDGREFTVGIIGNGNDLKVLPILEIDFSNLPEGLEHFYSHRVKHHYGEKTTYICPARLEESQRKKLENY
AKRAFKAIGLFDFARIDVRMRVNEFYIIEVNSLPLLVPVYSDLTKMLAPIGWKYEDLVNAILEAAIARYQKSSENSKLNL
G

Sequences:

>Translated_321_residues
MKVAVVYDLPMSEEDKRMVEAVIRALSKKHDSQPLPIDEKFMETVKEYDFAFNLSTGGGKLSKQVHAPAILDRLGVPFTG
SSAIVHAICIDKSITKLILEKSGIPTPRFVKIDDPNNIPEIDFYPAFVKPSCEGSALGISRDSVVKNQKELKKQVEMIMN
KFNQPAIVEEFIDGREFTVGIIGNGNDLKVLPILEIDFSNLPEGLEHFYSHRVKHHYGEKTTYICPARLEESQRKKLENY
AKRAFKAIGLFDFARIDVRMRVNEFYIIEVNSLPLLVPVYSDLTKMLAPIGWKYEDLVNAILEAAIARYQKSSENSKLNL
G
>Mature_321_residues
MKVAVVYDLPMSEEDKRMVEAVIRALSKKHDSQPLPIDEKFMETVKEYDFAFNLSTGGGKLSKQVHAPAILDRLGVPFTG
SSAIVHAICIDKSITKLILEKSGIPTPRFVKIDDPNNIPEIDFYPAFVKPSCEGSALGISRDSVVKNQKELKKQVEMIMN
KFNQPAIVEEFIDGREFTVGIIGNGNDLKVLPILEIDFSNLPEGLEHFYSHRVKHHYGEKTTYICPARLEESQRKKLENY
AKRAFKAIGLFDFARIDVRMRVNEFYIIEVNSLPLLVPVYSDLTKMLAPIGWKYEDLVNAILEAAIARYQKSSENSKLNL
G

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=249, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786579, Length=259, Percent_Identity=27.4131274131274, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4

Molecular weight: Translated: 36279; Mature: 36279

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVVYDLPMSEEDKRMVEAVIRALSKKHDSQPLPIDEKFMETVKEYDFAFNLSTGGGK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCCH
LSKQVHAPAILDRLGVPFTGSSAIVHAICIDKSITKLILEKSGIPTPRFVKIDDPNNIPE
HHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
IDFYPAFVKPSCEGSALGISRDSVVKNQKELKKQVEMIMNKFNQPAIVEEFIDGREFTVG
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEE
IIGNGNDLKVLPILEIDFSNLPEGLEHFYSHRVKHHYGEKTTYICPARLEESQRKKLENY
EEECCCCEEEEEEEECCHHCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHH
AKRAFKAIGLFDFARIDVRMRVNEFYIIEVNSLPLLVPVYSDLTKMLAPIGWKYEDLVNA
HHHHHHHHCHHHHEEEEEEEEECEEEEEEECCCEEEEHHHHHHHHHHHHCCCCHHHHHHH
ILEAAIARYQKSSENSKLNLG
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKVAVVYDLPMSEEDKRMVEAVIRALSKKHDSQPLPIDEKFMETVKEYDFAFNLSTGGGK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCCH
LSKQVHAPAILDRLGVPFTGSSAIVHAICIDKSITKLILEKSGIPTPRFVKIDDPNNIPE
HHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
IDFYPAFVKPSCEGSALGISRDSVVKNQKELKKQVEMIMNKFNQPAIVEEFIDGREFTVG
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEE
IIGNGNDLKVLPILEIDFSNLPEGLEHFYSHRVKHHYGEKTTYICPARLEESQRKKLENY
EEECCCCEEEEEEEECCHHCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHH
AKRAFKAIGLFDFARIDVRMRVNEFYIIEVNSLPLLVPVYSDLTKMLAPIGWKYEDLVNA
HHHHHHHHCHHHHEEEEEEEEECEEEEEEECCCEEEEHHHHHHHHHHHHCCCCHHHHHHH
ILEAAIARYQKSSENSKLNLG
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1018 [C]

Specific activity: 31

Km value (mM): 6 {D-Phe}} 9 {D-Met}} 15 {D-norleucine}} 40 {ATP}} 2.5 {ATP}} 2.4 {ATP}} 0.179 {ATP}} 0.161 {ATP}} 0.055 {ATP}} 0.049 {ATP}} 0.033 {ATP}} 0.008 {ATP}} 86 {D-Ala}} 70.3 {D-Ala}} 60.4 {D-Ala}} 57.5 {D-

Substrates: ATP; D-alanine

Specific reaction: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: [1 (S)-Aminoethyl] [(2RS) 2-carboxy-1-octyl] phosphinic acid; Aminoalkyl phosphinate; D-Ala-D-2-hydroxy; D-Ala-D-Ala; D-Ala-D-Alaphosphinate; D-Cycloserine; Gly; Gly-Gly; Phosphinates; Phosphonate dipeptide analogs [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]