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The map label for this gene is rpfG [H]

Identifier: 239617134

GI number: 239617134

Start: 788078

End: 789688

Strand: Reverse

Name: rpfG [H]

Synonym: Kole_0738

Alternate gene names: 239617134

Gene position: 789688-788078 (Counterclockwise)

Preceding gene: 239617135

Following gene: 239617125

Centisome position: 34.3

GC content: 38.61

Gene sequence:

>1611_bases
GTGGGAAGTTTTATAAAACATAACAGGATATTTCTGTTGACTATTGTGATTCTTTCTGTTCTCTTTTGGGTGGTTATACA
TTTTGGTGGCGAAAGAATATACGAAATCGAAGCGCAATGGCGGCATGAAAGTATTGTTTCTCAATTACTTACTACCGAAG
AGGTCATCAGGGTACTATTCCGTAATTTCGAACGAGATTTGCTCTTACTAAAAAACATTCCGAACACCAAAAACTTCGTG
AACAGTAATTTTGAATCTATTAGCCACAAAAACACAATTAAAGAAATATTCTATGAACTTGCCAAAGCCTCCAAGTGGTA
TTATCAAATAAGAATTATCGATTCTTCAGGCCAGGAAATAGTTAGAGTCAATCACAACCCGGATGGTACTACGATAATTG
TTCCCGATTCACGACTGCAAAATAAAGAGGACCGTTACTATTTTCAAGAAGCCATGAAAATGGATGAAAACCAGATCTAC
GTTTCTCCGATAGATTTGAACATTGAAAAAGGAAAGATAGAAATACCCTACACTCCGGTAATAAGGCTGGCAACCCCTTT
GTTTAATTCTGAAGGCAGAAAAATGGGGATTGTGATTCTAAATGTACGCTTTTCAGAAGTTCTTGAACTGTTATATAAAA
ATATGTTTATTCAGACGGAAGACGGATACTTAATATCGTTAAATCCAGACAAATCAGTTAATTTCCGTAAATCCAATTAC
AACTTCCCCAATGATTCTGGTCATATAGAGCTTTCAGATATAGAAACCTTACATTATTCAACTGTTGAATTTCTTCCTGG
CAATCGCTTGATCATTGCAGCATATCACAAACATCCAACATTGAAAGCGGCACTCCAAAACCTGACTTTGACGTCAACAC
TGGTGTTCATCTTATTTCTCACACTCATGCTGGTAGTTATTTATTTAAATTTATCGAAATCAAAAGATCTGATAGGTGCA
CAAAAAGCTATCATGTCTTCTCTTGCCACGCTTGCTGAACGAAGAGATACCGAAACAGGGCGCCATTTAAAAAGAACGAG
AGAGTACGCAGCGATACTGGCAAAACAACTGCGCAAAAACAAGAAATACCGGAAAATCATCACAAATGAATTCATTGAGA
ACCTTTACGATACCACCCCTTTGCACGACATAGGGAAGGTAGGGATACGGGATTCTATCCTGTTGAAGGAAGGAAAATTA
ACAGAACAGGAATATGAAGAGATGAAAAAACACACTTTAATAGGAAAACAGATACTTCAGGATGCTATCGATAAGTATCA
ACTGAAAGGCTCGTTTATTACTATGGCAAGAAACATCTGTGCGTATCACCATGAAAAGTACAACGGTGAGGGATATCCGG
AAGGCTTGAAAGGAGAAGAAATTCCCCTGGAAGCTAGAATTTTCGCCCTGTGCGATGCCTACGATGCAATCAGGTCGAAA
AGGCCCTACAAAGATGCGCTGCCGCACGAGGAAGCTCTCAGAAGAATAAAGGCAGATAGAGGTAAACACTTTGACCCCGA
TATTGTCGATGCTTTCCTGGAATGCGAAAAAGAGTTTGAAAGGGTAAGCAACATTTATAGTACAACCGCTGAAAATACCC
ATTCCCTTTAG

Upstream 100 bases:

>100_bases
CTGGGTTTATCTAGCTAAGTGCATAGTTTGGGAATTTAGTATACTTATCTCTTTACTCCCGATATATTTTCGTTACTATG
TTTCGAAAGAGGTTGATTGT

Downstream 100 bases:

>100_bases
GGAGTAGACAGCATGTCAAACGAGATCTATATTTTCCATACCACCAAAATAAGGTGCATTATAAAAAGGTAAAGCCTCCA
GAGAGTTTAGCTTCAAAACG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MGSFIKHNRIFLLTIVILSVLFWVVIHFGGERIYEIEAQWRHESIVSQLLTTEEVIRVLFRNFERDLLLLKNIPNTKNFV
NSNFESISHKNTIKEIFYELAKASKWYYQIRIIDSSGQEIVRVNHNPDGTTIIVPDSRLQNKEDRYYFQEAMKMDENQIY
VSPIDLNIEKGKIEIPYTPVIRLATPLFNSEGRKMGIVILNVRFSEVLELLYKNMFIQTEDGYLISLNPDKSVNFRKSNY
NFPNDSGHIELSDIETLHYSTVEFLPGNRLIIAAYHKHPTLKAALQNLTLTSTLVFILFLTLMLVVIYLNLSKSKDLIGA
QKAIMSSLATLAERRDTETGRHLKRTREYAAILAKQLRKNKKYRKIITNEFIENLYDTTPLHDIGKVGIRDSILLKEGKL
TEQEYEEMKKHTLIGKQILQDAIDKYQLKGSFITMARNICAYHHEKYNGEGYPEGLKGEEIPLEARIFALCDAYDAIRSK
RPYKDALPHEEALRRIKADRGKHFDPDIVDAFLECEKEFERVSNIYSTTAENTHSL

Sequences:

>Translated_536_residues
MGSFIKHNRIFLLTIVILSVLFWVVIHFGGERIYEIEAQWRHESIVSQLLTTEEVIRVLFRNFERDLLLLKNIPNTKNFV
NSNFESISHKNTIKEIFYELAKASKWYYQIRIIDSSGQEIVRVNHNPDGTTIIVPDSRLQNKEDRYYFQEAMKMDENQIY
VSPIDLNIEKGKIEIPYTPVIRLATPLFNSEGRKMGIVILNVRFSEVLELLYKNMFIQTEDGYLISLNPDKSVNFRKSNY
NFPNDSGHIELSDIETLHYSTVEFLPGNRLIIAAYHKHPTLKAALQNLTLTSTLVFILFLTLMLVVIYLNLSKSKDLIGA
QKAIMSSLATLAERRDTETGRHLKRTREYAAILAKQLRKNKKYRKIITNEFIENLYDTTPLHDIGKVGIRDSILLKEGKL
TEQEYEEMKKHTLIGKQILQDAIDKYQLKGSFITMARNICAYHHEKYNGEGYPEGLKGEEIPLEARIFALCDAYDAIRSK
RPYKDALPHEEALRRIKADRGKHFDPDIVDAFLECEKEFERVSNIYSTTAENTHSL
>Mature_535_residues
GSFIKHNRIFLLTIVILSVLFWVVIHFGGERIYEIEAQWRHESIVSQLLTTEEVIRVLFRNFERDLLLLKNIPNTKNFVN
SNFESISHKNTIKEIFYELAKASKWYYQIRIIDSSGQEIVRVNHNPDGTTIIVPDSRLQNKEDRYYFQEAMKMDENQIYV
SPIDLNIEKGKIEIPYTPVIRLATPLFNSEGRKMGIVILNVRFSEVLELLYKNMFIQTEDGYLISLNPDKSVNFRKSNYN
FPNDSGHIELSDIETLHYSTVEFLPGNRLIIAAYHKHPTLKAALQNLTLTSTLVFILFLTLMLVVIYLNLSKSKDLIGAQ
KAIMSSLATLAERRDTETGRHLKRTREYAAILAKQLRKNKKYRKIITNEFIENLYDTTPLHDIGKVGIRDSILLKEGKLT
EQEYEEMKKHTLIGKQILQDAIDKYQLKGSFITMARNICAYHHEKYNGEGYPEGLKGEEIPLEARIFALCDAYDAIRSKR
PYKDALPHEEALRRIKADRGKHFDPDIVDAFLECEKEFERVSNIYSTTAENTHSL

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 62236; Mature: 62104

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFIKHNRIFLLTIVILSVLFWVVIHFGGERIYEIEAQWRHESIVSQLLTTEEVIRVLF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
RNFERDLLLLKNIPNTKNFVNSNFESISHKNTIKEIFYELAKASKWYYQIRIIDSSGQEI
HCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEE
VRVNHNPDGTTIIVPDSRLQNKEDRYYFQEAMKMDENQIYVSPIDLNIEKGKIEIPYTPV
EEEECCCCCCEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCEEECCCCCH
IRLATPLFNSEGRKMGIVILNVRFSEVLELLYKNMFIQTEDGYLISLNPDKSVNFRKSNY
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCEEEECCCEEEEECCCCCCCEEECCC
NFPNDSGHIELSDIETLHYSTVEFLPGNRLIIAAYHKHPTLKAALQNLTLTSTLVFILFL
CCCCCCCCEEEECCCCHHHHHEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TLMLVVIYLNLSKSKDLIGAQKAIMSSLATLAERRDTETGRHLKRTREYAAILAKQLRKN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
KKYRKIITNEFIENLYDTTPLHDIGKVGIRDSILLKEGKLTEQEYEEMKKHTLIGKQILQ
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
DAIDKYQLKGSFITMARNICAYHHEKYNGEGYPEGLKGEEIPLEARIFALCDAYDAIRSK
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RPYKDALPHEEALRRIKADRGKHFDPDIVDAFLECEKEFERVSNIYSTTAENTHSL
CCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GSFIKHNRIFLLTIVILSVLFWVVIHFGGERIYEIEAQWRHESIVSQLLTTEEVIRVLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
RNFERDLLLLKNIPNTKNFVNSNFESISHKNTIKEIFYELAKASKWYYQIRIIDSSGQEI
HCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEE
VRVNHNPDGTTIIVPDSRLQNKEDRYYFQEAMKMDENQIYVSPIDLNIEKGKIEIPYTPV
EEEECCCCCCEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCEEECCCCCH
IRLATPLFNSEGRKMGIVILNVRFSEVLELLYKNMFIQTEDGYLISLNPDKSVNFRKSNY
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCEEEECCCEEEEECCCCCCCEEECCC
NFPNDSGHIELSDIETLHYSTVEFLPGNRLIIAAYHKHPTLKAALQNLTLTSTLVFILFL
CCCCCCCCEEEECCCCHHHHHEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
TLMLVVIYLNLSKSKDLIGAQKAIMSSLATLAERRDTETGRHLKRTREYAAILAKQLRKN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
KKYRKIITNEFIENLYDTTPLHDIGKVGIRDSILLKEGKLTEQEYEEMKKHTLIGKQILQ
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
DAIDKYQLKGSFITMARNICAYHHEKYNGEGYPEGLKGEEIPLEARIFALCDAYDAIRSK
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RPYKDALPHEEALRRIKADRGKHFDPDIVDAFLECEKEFERVSNIYSTTAENTHSL
CCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]