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The map label for this gene is gldA [H]

Identifier: 239616909

GI number: 239616909

Start: 551927

End: 553021

Strand: Direct

Name: gldA [H]

Synonym: Kole_0505

Alternate gene names: 239616909

Gene position: 551927-553021 (Clockwise)

Preceding gene: 239616894

Following gene: 239616910

Centisome position: 23.97

GC content: 46.94

Gene sequence:

>1095_bases
ATGATCAGTACGACTATTTTTCCGGGTCGCTACGTTCAGGGTAGCGGTGCTTTGGAAAGATTAGGATCGGAAATGGTACG
ATTTGGCAAGAAGGGATTCGTAATCTGTGATTCTTTCGTTTTAGAAAATCTTTTGCCTGACTTCAGGCAGAGTATGGAAC
AGTCTCTGGAAGTAGTTGTAGAAAAATTCGGCGGTGAATGCTCTGATGAAGAAATAAGCAGGCTGGCTGACCTGGCCAAA
GGTGCCGGTTGCGAACTCATCGTTGGTATAGGTGGAGGCAAAACGCTCGATACGGCCAAAGCAGTTGCCTACGAACTAAA
GCTCCCTGTCGTCATCGTTCCAACCCTTGCTTCAACAGACGCACCTTGTAGCGCTCTTTCAGTGATATATACACCCAACG
GAGAATTCAAAAGATATCTGATTCTGCCTAAAAACCCCGATCTTGTATTGGTAGACACCAAAATAATTGCTCAAGCACCA
GCCAGATTCCTGGTTTCAGGTATGGGTGATGCACTGGCTACCTGGTTCGAAGCTGAATCGTGCATGAAAAAGTACGCCAA
AAACATGACCGGTAACGTAGGTTCTATGACCGCTTACGCTCTGGCAAAGCTCTGCTATGAGACCCTGCTTGAATACGGTG
TTGCTGCAAAATCATCTTGTGAAGCCAACGCCGTAACACCCGCTTTAGAACACATCGTAGAAGCAAACACACTGCTGAGT
GGCTTGGGTTTTGAAAGCGGCGGCTTGGCTGCAGCACACGCTATCCACAATGGTCTTACGGTATTAGAGCAAACTCACAA
ATATTATCACGGTGAAAAAGTTGCATTTGGTACCCTCACGTCCCTGTTCCTTACAGATAAACCAAGAGAAATCATTGATG
AGGTCTATTCTTTCTGTGAATCCGTAGGCTTGCCCACCACACTTGCAGACATTGGGCTCGACGGTGTTTCAGACGAGGAA
CTCATGAAAGTAGCGGAAGCGGCATGTGCCGAAGGGGAAACCATACACAACGAACCCATTCCTGTCACTCCTGAAGCTGT
TTATTCTGCTATCAAAACCGCAGACGCTGAAGGTAAAAGGAGAAAAGCGGTTTGA

Upstream 100 bases:

>100_bases
CTAACCTGAAAAAATAAAGAAAACTTTTCTGTGTTAAAGTGAAGATGAAAAAACAAAAAGATATAAGTACCATATCCCAA
CCAAAAGGGAGGTAAAAAGC

Downstream 100 bases:

>100_bases
TTGAAGTAGGTTTACTCACAGGTGGTCAAATAAATAAAGATATGTTCCCTGGATAACGAAGAGTCAATTTTAAATTTTAT
TTTACGGCTTAATTTGGAGT

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MISTTIFPGRYVQGSGALERLGSEMVRFGKKGFVICDSFVLENLLPDFRQSMEQSLEVVVEKFGGECSDEEISRLADLAK
GAGCELIVGIGGGKTLDTAKAVAYELKLPVVIVPTLASTDAPCSALSVIYTPNGEFKRYLILPKNPDLVLVDTKIIAQAP
ARFLVSGMGDALATWFEAESCMKKYAKNMTGNVGSMTAYALAKLCYETLLEYGVAAKSSCEANAVTPALEHIVEANTLLS
GLGFESGGLAAAHAIHNGLTVLEQTHKYYHGEKVAFGTLTSLFLTDKPREIIDEVYSFCESVGLPTTLADIGLDGVSDEE
LMKVAEAACAEGETIHNEPIPVTPEAVYSAIKTADAEGKRRKAV

Sequences:

>Translated_364_residues
MISTTIFPGRYVQGSGALERLGSEMVRFGKKGFVICDSFVLENLLPDFRQSMEQSLEVVVEKFGGECSDEEISRLADLAK
GAGCELIVGIGGGKTLDTAKAVAYELKLPVVIVPTLASTDAPCSALSVIYTPNGEFKRYLILPKNPDLVLVDTKIIAQAP
ARFLVSGMGDALATWFEAESCMKKYAKNMTGNVGSMTAYALAKLCYETLLEYGVAAKSSCEANAVTPALEHIVEANTLLS
GLGFESGGLAAAHAIHNGLTVLEQTHKYYHGEKVAFGTLTSLFLTDKPREIIDEVYSFCESVGLPTTLADIGLDGVSDEE
LMKVAEAACAEGETIHNEPIPVTPEAVYSAIKTADAEGKRRKAV
>Mature_364_residues
MISTTIFPGRYVQGSGALERLGSEMVRFGKKGFVICDSFVLENLLPDFRQSMEQSLEVVVEKFGGECSDEEISRLADLAK
GAGCELIVGIGGGKTLDTAKAVAYELKLPVVIVPTLASTDAPCSALSVIYTPNGEFKRYLILPKNPDLVLVDTKIIAQAP
ARFLVSGMGDALATWFEAESCMKKYAKNMTGNVGSMTAYALAKLCYETLLEYGVAAKSSCEANAVTPALEHIVEANTLLS
GLGFESGGLAAAHAIHNGLTVLEQTHKYYHGEKVAFGTLTSLFLTDKPREIIDEVYSFCESVGLPTTLADIGLDGVSDEE
LMKVAEAACAEGETIHNEPIPVTPEAVYSAIKTADAEGKRRKAV

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=354, Percent_Identity=55.3672316384181, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786815, Length=299, Percent_Identity=35.1170568561873, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38864; Mature: 38864

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISTTIFPGRYVQGSGALERLGSEMVRFGKKGFVICDSFVLENLLPDFRQSMEQSLEVVV
CCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
EKFGGECSDEEISRLADLAKGAGCELIVGIGGGKTLDTAKAVAYELKLPVVIVPTLASTD
HHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCC
APCSALSVIYTPNGEFKRYLILPKNPDLVLVDTKIIAQAPARFLVSGMGDALATWFEAES
CCCCEEEEEECCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CMKKYAKNMTGNVGSMTAYALAKLCYETLLEYGVAAKSSCEANAVTPALEHIVEANTLLS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GLGFESGGLAAAHAIHNGLTVLEQTHKYYHGEKVAFGTLTSLFLTDKPREIIDEVYSFCE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHH
SVGLPTTLADIGLDGVSDEELMKVAEAACAEGETIHNEPIPVTPEAVYSAIKTADAEGKR
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
RKAV
CCCC
>Mature Secondary Structure
MISTTIFPGRYVQGSGALERLGSEMVRFGKKGFVICDSFVLENLLPDFRQSMEQSLEVVV
CCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
EKFGGECSDEEISRLADLAKGAGCELIVGIGGGKTLDTAKAVAYELKLPVVIVPTLASTD
HHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCC
APCSALSVIYTPNGEFKRYLILPKNPDLVLVDTKIIAQAPARFLVSGMGDALATWFEAES
CCCCEEEEEECCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CMKKYAKNMTGNVGSMTAYALAKLCYETLLEYGVAAKSSCEANAVTPALEHIVEANTLLS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GLGFESGGLAAAHAIHNGLTVLEQTHKYYHGEKVAFGTLTSLFLTDKPREIIDEVYSFCE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHH
SVGLPTTLADIGLDGVSDEELMKVAEAACAEGETIHNEPIPVTPEAVYSAIKTADAEGKR
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
RKAV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571; 12193646 [H]