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The map label for this gene is thiH [H]

Identifier: 239616801

GI number: 239616801

Start: 413150

End: 414550

Strand: Direct

Name: thiH [H]

Synonym: Kole_0394

Alternate gene names: 239616801

Gene position: 413150-414550 (Clockwise)

Preceding gene: 239616800

Following gene: 239616802

Centisome position: 17.95

GC content: 41.61

Gene sequence:

>1401_bases
ATGAGAACGGAAGTGAGAAGTTTCATCAACGTTGAAGAAATCGAAGAGCACTTGGAGAAAAATTCTTTTCCCAGTAGAGA
AAGAATCGAGGAAATAATTCAAAAGTCTCTCGACAAAAATCGACTGAATCCAGACGAGGTAGCAGCATTGTTGAACGTCA
GAGATCCTGAACTGAGAGAACTAATCTTCGAAGGTGCAAGAGAACTGAAAAAACGGGTATATGGGAACAGAATTGTCCTT
TTTGCACCATTGTACATCGGAAATAAGTGCATCAACAATTGTGTCTACTGTGGTTTCAGGGTTAGCAACAAGGAGGTTGT
AAGGCGCACACTCAATTTCGATGAACTCAAACTCGAAACGAAAGCCCTTGTAAACAGGGGACACAAAAGGCTGATACTCG
TTTATGGAGAACACCCTGATTACAATGCGGATTTCATAGCAAAAACCGTCGAAATCGTTTACTCCATCAAAGAAGGAAAT
GGCGAAATCAGGCGTGTTAATATCAATGCAGCTCCGTTAGAGGTCGAGGATTATAAAAAAGTTAAAGAAGCCGGGATTGG
AACCTATCAGATTTTCCAGGAAACTTACCACCCTGGAGTATATAAAAAAATGCACCCAACAGGACCAAAAGCCGATTATC
TTTACAGGTTATACGGTCTTGACAGAGCCATGGAAGCAGGGATAGATGATGTTGGAATTGGTGCTCTTTTCGGACTTTAC
GATTGGAAATTTGAAGTTATGGGATTACTTTATCACACAATACATCTTGAAGAACGTTTTGGAGTAGGGCCGCACACGAT
ATCGTTCCCAAGAATCGAACCAGCAATAGGAACTCCTATCTCCTCGAAACCACCGTTTTCCGTAAATGATGAGGATTTCA
AACATCTTGTCGCTACTTTGCGATTAGCTGTGCCTTATACTGGCATGATCCTCACAGCGAGAGAACCCATAGAGATACGC
AGGGAAGTCTTAAAACTTGGGGTATCTCAAATAGATGCAGGTTCAAATATCGGAATAGGGGCTTATTCTAAAGAAGATAA
AGAAGCTTATAAAAAATCCCAATTTACCCTTGGGGACACGCGACCGTTGGATGTCGTTATAAAGGAACTCGCACAGGAAG
GTTACATCCCTTCTTTTTGTACAGCCTGCTACAGATTAGGTAGAACTGGCGAACACTTTATGGAATTTGCTATCCCTGGA
TTTGTTAAACGCTTCTGCCAACCAAATGCTGTCCTTACATTCGCCGAGTACCTGGCAGATTACGCTTCAGAGGAAACAAA
GGAAGTAGGCAGCAAACTGATAAAAAAAGAGATAAACAACGTCGAGGATGAGAAATTAAAAGAAACCCTGAGGCGGTACT
ATGAGAGAGTTCTCAATGGAGAAAGAGATCTGTATTTCTAA

Upstream 100 bases:

>100_bases
GGGTGGATCGGAAAAATGAATTCAATAGGAACTAAATTAGTTCCTTTGAAGATAATCCATCCCCGGCTTTGCCGGGGATT
TTTGTTTGGAGGGGTAAACA

Downstream 100 bases:

>100_bases
GATCTTAGAAATTCTAGAAAGGTTCGCAAATAAAGATACAAAAGTATGGATAGCCGAAAAATTTGGCAGACGTTGGTCCT
ATATCAAGGGATTCGGCGAG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRTEVRSFINVEEIEEHLEKNSFPSRERIEEIIQKSLDKNRLNPDEVAALLNVRDPELRELIFEGARELKKRVYGNRIVL
FAPLYIGNKCINNCVYCGFRVSNKEVVRRTLNFDELKLETKALVNRGHKRLILVYGEHPDYNADFIAKTVEIVYSIKEGN
GEIRRVNINAAPLEVEDYKKVKEAGIGTYQIFQETYHPGVYKKMHPTGPKADYLYRLYGLDRAMEAGIDDVGIGALFGLY
DWKFEVMGLLYHTIHLEERFGVGPHTISFPRIEPAIGTPISSKPPFSVNDEDFKHLVATLRLAVPYTGMILTAREPIEIR
REVLKLGVSQIDAGSNIGIGAYSKEDKEAYKKSQFTLGDTRPLDVVIKELAQEGYIPSFCTACYRLGRTGEHFMEFAIPG
FVKRFCQPNAVLTFAEYLADYASEETKEVGSKLIKKEINNVEDEKLKETLRRYYERVLNGERDLYF

Sequences:

>Translated_466_residues
MRTEVRSFINVEEIEEHLEKNSFPSRERIEEIIQKSLDKNRLNPDEVAALLNVRDPELRELIFEGARELKKRVYGNRIVL
FAPLYIGNKCINNCVYCGFRVSNKEVVRRTLNFDELKLETKALVNRGHKRLILVYGEHPDYNADFIAKTVEIVYSIKEGN
GEIRRVNINAAPLEVEDYKKVKEAGIGTYQIFQETYHPGVYKKMHPTGPKADYLYRLYGLDRAMEAGIDDVGIGALFGLY
DWKFEVMGLLYHTIHLEERFGVGPHTISFPRIEPAIGTPISSKPPFSVNDEDFKHLVATLRLAVPYTGMILTAREPIEIR
REVLKLGVSQIDAGSNIGIGAYSKEDKEAYKKSQFTLGDTRPLDVVIKELAQEGYIPSFCTACYRLGRTGEHFMEFAIPG
FVKRFCQPNAVLTFAEYLADYASEETKEVGSKLIKKEINNVEDEKLKETLRRYYERVLNGERDLYF
>Mature_466_residues
MRTEVRSFINVEEIEEHLEKNSFPSRERIEEIIQKSLDKNRLNPDEVAALLNVRDPELRELIFEGARELKKRVYGNRIVL
FAPLYIGNKCINNCVYCGFRVSNKEVVRRTLNFDELKLETKALVNRGHKRLILVYGEHPDYNADFIAKTVEIVYSIKEGN
GEIRRVNINAAPLEVEDYKKVKEAGIGTYQIFQETYHPGVYKKMHPTGPKADYLYRLYGLDRAMEAGIDDVGIGALFGLY
DWKFEVMGLLYHTIHLEERFGVGPHTISFPRIEPAIGTPISSKPPFSVNDEDFKHLVATLRLAVPYTGMILTAREPIEIR
REVLKLGVSQIDAGSNIGIGAYSKEDKEAYKKSQFTLGDTRPLDVVIKELAQEGYIPSFCTACYRLGRTGEHFMEFAIPG
FVKRFCQPNAVLTFAEYLADYASEETKEVGSKLIKKEINNVEDEKLKETLRRYYERVLNGERDLYF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=29.7814207650273, Blast_Score=178, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53491; Mature: 53491

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEVRSFINVEEIEEHLEKNSFPSRERIEEIIQKSLDKNRLNPDEVAALLNVRDPELRE
CCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
LIFEGARELKKRVYGNRIVLFAPLYIGNKCINNCVYCGFRVSNKEVVRRTLNFDELKLET
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHEEECEEECCHHHHHHHCCHHHHHHHH
KALVNRGHKRLILVYGEHPDYNADFIAKTVEIVYSIKEGNGEIRRVNINAAPLEVEDYKK
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
VKEAGIGTYQIFQETYHPGVYKKMHPTGPKADYLYRLYGLDRAMEAGIDDVGIGALFGLY
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DWKFEVMGLLYHTIHLEERFGVGPHTISFPRIEPAIGTPISSKPPFSVNDEDFKHLVATL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLAVPYTGMILTAREPIEIRREVLKLGVSQIDAGSNIGIGAYSKEDKEAYKKSQFTLGDT
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
RPLDVVIKELAQEGYIPSFCTACYRLGRTGEHFMEFAIPGFVKRFCQPNAVLTFAEYLAD
CHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YASEETKEVGSKLIKKEINNVEDEKLKETLRRYYERVLNGERDLYF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRTEVRSFINVEEIEEHLEKNSFPSRERIEEIIQKSLDKNRLNPDEVAALLNVRDPELRE
CCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
LIFEGARELKKRVYGNRIVLFAPLYIGNKCINNCVYCGFRVSNKEVVRRTLNFDELKLET
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHEEECEEECCHHHHHHHCCHHHHHHHH
KALVNRGHKRLILVYGEHPDYNADFIAKTVEIVYSIKEGNGEIRRVNINAAPLEVEDYKK
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
VKEAGIGTYQIFQETYHPGVYKKMHPTGPKADYLYRLYGLDRAMEAGIDDVGIGALFGLY
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DWKFEVMGLLYHTIHLEERFGVGPHTISFPRIEPAIGTPISSKPPFSVNDEDFKHLVATL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLAVPYTGMILTAREPIEIRREVLKLGVSQIDAGSNIGIGAYSKEDKEAYKKSQFTLGDT
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
RPLDVVIKELAQEGYIPSFCTACYRLGRTGEHFMEFAIPGFVKRFCQPNAVLTFAEYLAD
CHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YASEETKEVGSKLIKKEINNVEDEKLKETLRRYYERVLNGERDLYF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]