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The map label for this gene is prpE [H]

Identifier: 239616799

GI number: 239616799

Start: 410749

End: 411375

Strand: Direct

Name: prpE [H]

Synonym: Kole_0392

Alternate gene names: 239616799

Gene position: 410749-411375 (Clockwise)

Preceding gene: 239616798

Following gene: 239616800

Centisome position: 17.84

GC content: 38.92

Gene sequence:

>627_bases
ATGATTTATGCCATAGGCGATATCCACGGTTGCCTCGAAAGTCTTAAAAAGCTCATAGAAAAAATAAATCCTTCGCGCAC
TGATATGTTGATATTCGTTGGAGATTATATCGACAGAGGCCCCAATTCAAAAGGAGTAGTCGATTTTCTTATAAAATTAT
CCAGAAGTCGAAAATGTATTTTCATTCGTGGTAATCACGAGCAAATGTTGCTCGATTACCTGAACGGTGGAAAAACAAGA
AAGTTGTGGCATTTTAATGGAATGGAATATACGATAAACAGCTATGGTGGGATAGATAAAATCCCAGAGAAACACATTCG
CTTCTTCGAGAAAACCATCCTTTTCCATGAAACCGATGAATATGTTTTCGTTCACGCGGGCGTTCGACCGGGTATTCCGT
TAAACAAGCAAAAAGACGGAGATCTTCTATGGATAAGAGATGAATTTATATACGCTGAATGGCCAGTAAAAGACAAAGTA
GTCATTTTTGGTCACACACCAGTTCTCCAGGAACCTCTGATTATGAAGGACAAGATTGGTATTGATACCGGATGTGTTTA
TGGTGGAAAGCTAACCGCTCTTAGGTTGGATGACATGTGTTTCTTCTCAGTTAAGTCTATCTTCTAA

Upstream 100 bases:

>100_bases
TTTTTCCAAAGAAAAAAAGATCGAGTGGTTCATAAGTCCCACAGTGGGCATCGTGATTTCTATAGTATGGAAAATTACAA
TGCTGGGAGTATTCTGATAC

Downstream 100 bases:

>100_bases
CGTGGATGTTTTTTGGGGGGGATAGGATGAGTCATTATTCAAAAGTAAAAAGTGTCACGATGCAGGGACTTAAGGCCATC
GAAATACTCGTGGAGGTGGA

Product: metallophosphoesterase

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIYAIGDIHGCLESLKKLIEKINPSRTDMLIFVGDYIDRGPNSKGVVDFLIKLSRSRKCIFIRGNHEQMLLDYLNGGKTR
KLWHFNGMEYTINSYGGIDKIPEKHIRFFEKTILFHETDEYVFVHAGVRPGIPLNKQKDGDLLWIRDEFIYAEWPVKDKV
VIFGHTPVLQEPLIMKDKIGIDTGCVYGGKLTALRLDDMCFFSVKSIF

Sequences:

>Translated_208_residues
MIYAIGDIHGCLESLKKLIEKINPSRTDMLIFVGDYIDRGPNSKGVVDFLIKLSRSRKCIFIRGNHEQMLLDYLNGGKTR
KLWHFNGMEYTINSYGGIDKIPEKHIRFFEKTILFHETDEYVFVHAGVRPGIPLNKQKDGDLLWIRDEFIYAEWPVKDKV
VIFGHTPVLQEPLIMKDKIGIDTGCVYGGKLTALRLDDMCFFSVKSIF
>Mature_208_residues
MIYAIGDIHGCLESLKKLIEKINPSRTDMLIFVGDYIDRGPNSKGVVDFLIKLSRSRKCIFIRGNHEQMLLDYLNGGKTR
KLWHFNGMEYTINSYGGIDKIPEKHIRFFEKTILFHETDEYVFVHAGVRPGIPLNKQKDGDLLWIRDEFIYAEWPVKDKV
VIFGHTPVLQEPLIMKDKIGIDTGCVYGGKLTALRLDDMCFFSVKSIF

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=209, Percent_Identity=34.4497607655502, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 23990; Mature: 23990

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYAIGDIHGCLESLKKLIEKINPSRTDMLIFVGDYIDRGPNSKGVVDFLIKLSRSRKCI
CEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCCHHHHHHHHCCCCEEE
FIRGNHEQMLLDYLNGGKTRKLWHFNGMEYTINSYGGIDKIPEKHIRFFEKTILFHETDE
EEECCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHEEEECCC
YVFVHAGVRPGIPLNKQKDGDLLWIRDEFIYAEWPVKDKVVIFGHTPVLQEPLIMKDKIG
EEEEECCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCEEEEEECCCCCCCCCEEECCCC
IDTGCVYGGKLTALRLDDMCFFSVKSIF
CCCCEEECCEEEEEEECCEEEEEHHHCC
>Mature Secondary Structure
MIYAIGDIHGCLESLKKLIEKINPSRTDMLIFVGDYIDRGPNSKGVVDFLIKLSRSRKCI
CEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCCHHHHHHHHCCCCEEE
FIRGNHEQMLLDYLNGGKTRKLWHFNGMEYTINSYGGIDKIPEKHIRFFEKTILFHETDE
EEECCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHEEEECCC
YVFVHAGVRPGIPLNKQKDGDLLWIRDEFIYAEWPVKDKVVIFGHTPVLQEPLIMKDKIG
EEEEECCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCEEEEEECCCCCCCCCEEECCCC
IDTGCVYGGKLTALRLDDMCFFSVKSIF
CCCCEEECCEEEEEEECCEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA