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The map label for this gene is phoP [H]

Identifier: 239616779

GI number: 239616779

Start: 390211

End: 390837

Strand: Direct

Name: phoP [H]

Synonym: Kole_0372

Alternate gene names: 239616779

Gene position: 390211-390837 (Clockwise)

Preceding gene: 239616778

Following gene: 239616780

Centisome position: 16.95

GC content: 39.23

Gene sequence:

>627_bases
ATGATGGCAAAAATTTTTGTCATAGATAACACATTGAGTATCAGACGATTAATGAAAGACATCCTTGAAAGAGAAGGATA
CGAGGTTGAGAGTTTCGCAACCGCCGAAGAAGCTCTTCATGAGATAGAGAAAAATGAACCTGATCTGGTGATGGTAGATT
TGAGATTACCATCTATGGATGGTATTACCTTTATAAAAACACTGGAAGAGAATGGTTTCATTTTCCCGATTGTCGTTGTA
TCGGATATTACTTCGCCGGAAGCCATTACCGAAGCTTTCAGACATGGGATTTGCGATTTTATCAGTAAGCCTTTTTCGCC
TGAAGAAATTGGAAGTGCTGTAGAAAGGTGCATCAAGAATGATGAAAGTCTCCAAAAACGCGCCAGAGAAATAGAGCGCA
TGCTGGAAAAAAGTGACTTTAGAAGGGCATTGAAACATATAAGCAAATTGTTTTCCGATTTTCCAAACTCTGCTTATCCG
CATTTTCTTTACGCTTTGGCTCTCAAAGAATCAAGACGCGGGGAAGCAATACGTCATTTAAAAGCAGCTTTAGCTCTTGA
ACCAGGCTTTATGAGAGCGAAAAATGAACTCGAAAAACTTGAAAATTCCGGGGATGGTGAAAAATAA

Upstream 100 bases:

>100_bases
CGTAAATTATTTGATTAAACCCTCATGAAATAACATATTAACCAGATTCAAACAGGTTTATCACTTTATGTGGTAATATG
TTTTGTGTTTGGAGGTGATT

Downstream 100 bases:

>100_bases
ATGCCAATTGTCAAAGGTGCATTGAAAAAGAAAGGGTACTTCGTTGTTATCTTCGGTTGTGGAAGATTGGGATCGAGAAT
TGCCAACTGGTTATCTTCAA

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MMAKIFVIDNTLSIRRLMKDILEREGYEVESFATAEEALHEIEKNEPDLVMVDLRLPSMDGITFIKTLEENGFIFPIVVV
SDITSPEAITEAFRHGICDFISKPFSPEEIGSAVERCIKNDESLQKRAREIERMLEKSDFRRALKHISKLFSDFPNSAYP
HFLYALALKESRRGEAIRHLKAALALEPGFMRAKNELEKLENSGDGEK

Sequences:

>Translated_208_residues
MMAKIFVIDNTLSIRRLMKDILEREGYEVESFATAEEALHEIEKNEPDLVMVDLRLPSMDGITFIKTLEENGFIFPIVVV
SDITSPEAITEAFRHGICDFISKPFSPEEIGSAVERCIKNDESLQKRAREIERMLEKSDFRRALKHISKLFSDFPNSAYP
HFLYALALKESRRGEAIRHLKAALALEPGFMRAKNELEKLENSGDGEK
>Mature_208_residues
MMAKIFVIDNTLSIRRLMKDILEREGYEVESFATAEEALHEIEKNEPDLVMVDLRLPSMDGITFIKTLEENGFIFPIVVV
SDITSPEAITEAFRHGICDFISKPFSPEEIGSAVERCIKNDESLQKRAREIERMLEKSDFRRALKHISKLFSDFPNSAYP
HFLYALALKESRRGEAIRHLKAALALEPGFMRAKNELEKLENSGDGEK

Specific function: Member of the two-component regulatory system phoP/phoR involved in the regulation of alkaline phosphatase genes phoA and phoB and of phosphodiesterase [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=26.5625, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=28.1481481481481, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787375, Length=121, Percent_Identity=26.4462809917355, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=25.8333333333333, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=29.6610169491525, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=27.5862068965517, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 23778; Mature: 23778

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKIFVIDNTLSIRRLMKDILEREGYEVESFATAEEALHEIEKNEPDLVMVDLRLPSMD
CCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GITFIKTLEENGFIFPIVVVSDITSPEAITEAFRHGICDFISKPFSPEEIGSAVERCIKN
CHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
DESLQKRAREIERMLEKSDFRRALKHISKLFSDFPNSAYPHFLYALALKESRRGEAIRHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KAALALEPGFMRAKNELEKLENSGDGEK
HHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMAKIFVIDNTLSIRRLMKDILEREGYEVESFATAEEALHEIEKNEPDLVMVDLRLPSMD
CCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GITFIKTLEENGFIFPIVVVSDITSPEAITEAFRHGICDFISKPFSPEEIGSAVERCIKN
CHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
DESLQKRAREIERMLEKSDFRRALKHISKLFSDFPNSAYPHFLYALALKESRRGEAIRHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KAALALEPGFMRAKNELEKLENSGDGEK
HHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1454550; 3036763; 9387221; 9384377; 8045898; 3142862 [H]