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The map label for this gene is glnA [H]

Identifier: 239616697

GI number: 239616697

Start: 314642

End: 315967

Strand: Reverse

Name: glnA [H]

Synonym: Kole_0290

Alternate gene names: 239616697

Gene position: 315967-314642 (Counterclockwise)

Preceding gene: 239616699

Following gene: 239616695

Centisome position: 13.73

GC content: 40.87

Gene sequence:

>1326_bases
ATGGATAAGAAATCTGTTCTTGAGCTGGTTAAAAGGGAAAACGTAAGGTTTGTAAGGTTGCAAATCACAGACATCAACGG
CTTTTTGAAAAACGTAGAGATACCTGTGAAGCTTCTGGAGAAGAGTTTAGATGAAGGAGTTATGTTCGACGGTTCTTCAA
TTGATGGGTTTGTGAGAATCGAAGAATCGGATATGTACCTTATTCCTGATGCATCAACTGTGGCCCTTCTTCCGTGGAAA
GATGATGATGAAAAAACGATGAGAATTATCTGTGATGTTCACCTTCCAAATGGTGAACCATTCGAAGGAGATCCAAGATA
TAGGTTGAAGAAAGTCGTACAAAGAGCTAAAGAAATGGGATTTATTGCCCACGCCGGACCTGAGCCTGAATTTTTTCTCT
TCAAGAGGGATGAAAATTCTGATAGGCCCATCGTTGGATTTATGGACAGGGGAAGTTATTTCGATATGCTGCCGGTGGAT
AAAGGGGAAGAGACGAGAAAAGAGATAGTTATATCTCTTGAAAAGATGGGATTTGATATTGAAGCGGCTCACCATGAAGT
TGCACCTTCACAGCACGAGATTGATTTTCGATATGCCGATATCCTGAAAACGGCTGATAACATTCAAACATTCAAATGGG
TTGTTAAAACGATAGCGCTCATGAACGGTTTATACGCGACCTTTATGCCAAAGCCCTTCGCTCAGGTTAATGGTTCGGGG
ATGCATATTCATTTGAGTTTATTTGAAAACGGGAAGAACGCTTTCTATGATCCTGAAAAACCCTATGAGCTCAGTGACGT
TTTGCTTAATTTTGTTGGTGGAATCATAAAGTATGCGAAAGAATTGACGATAGTAACCAATCCAATAATTAACAGTTACA
AGCGGCTTGTTCCGGGCTACGAAGCACCAATTAACATTTCCTGGGCTCTCGGTAATCGAAGCGCTATGATTCGCGTTCCA
GCGGCTCGAGGTCAGGCAACGAGAATAGAAATCAGAAATCCTGATCCTTCGTGTAACCCTTATCTAGCTTTAGCTGTTTT
GCTGGAAGCTGGGCTAAGAGGCATTGAGGAAGGGATCTTGCCGCCTAAACCAGTGGGAGAGAACATATTCAAGCTCAATC
AAACAGAAAGAAAAAAGCGGAAGATTCAGAATCTCCCGGGCAATTTGAATGAAGCACTGGAATATTTTTCTCGAAGTTCT
CTGATCAAAGATGTACTTGGGGATCATATTTATAAGAAATTTCTCGAGACCAAGAAAAAAGAATGGAAAGAATTCATGAT
GCACGTTACTGATTGGGAAAAAGAGAAATATCTTGAACTTTACTAA

Upstream 100 bases:

>100_bases
TTTTATCAAACGTGAACGTGTTTTCCTTGACTTTCTTCTCTGAATTTGTGTAAATTTGAAGCAATAGTTCATGTATTTCT
TTAGATTCAGGGGTGATTGA

Downstream 100 bases:

>100_bases
AGAGATTGAAAAATAAGCCGCTCTCTGTTTCAACGGGGAGCGGCTTGTTCATTTAAAAATTGCTCAGGTATGAACTTTTT
CATGAAGTCACTTTCTTTTC

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MDKKSVLELVKRENVRFVRLQITDINGFLKNVEIPVKLLEKSLDEGVMFDGSSIDGFVRIEESDMYLIPDASTVALLPWK
DDDEKTMRIICDVHLPNGEPFEGDPRYRLKKVVQRAKEMGFIAHAGPEPEFFLFKRDENSDRPIVGFMDRGSYFDMLPVD
KGEETRKEIVISLEKMGFDIEAAHHEVAPSQHEIDFRYADILKTADNIQTFKWVVKTIALMNGLYATFMPKPFAQVNGSG
MHIHLSLFENGKNAFYDPEKPYELSDVLLNFVGGIIKYAKELTIVTNPIINSYKRLVPGYEAPINISWALGNRSAMIRVP
AARGQATRIEIRNPDPSCNPYLALAVLLEAGLRGIEEGILPPKPVGENIFKLNQTERKKRKIQNLPGNLNEALEYFSRSS
LIKDVLGDHIYKKFLETKKKEWKEFMMHVTDWEKEKYLELY

Sequences:

>Translated_441_residues
MDKKSVLELVKRENVRFVRLQITDINGFLKNVEIPVKLLEKSLDEGVMFDGSSIDGFVRIEESDMYLIPDASTVALLPWK
DDDEKTMRIICDVHLPNGEPFEGDPRYRLKKVVQRAKEMGFIAHAGPEPEFFLFKRDENSDRPIVGFMDRGSYFDMLPVD
KGEETRKEIVISLEKMGFDIEAAHHEVAPSQHEIDFRYADILKTADNIQTFKWVVKTIALMNGLYATFMPKPFAQVNGSG
MHIHLSLFENGKNAFYDPEKPYELSDVLLNFVGGIIKYAKELTIVTNPIINSYKRLVPGYEAPINISWALGNRSAMIRVP
AARGQATRIEIRNPDPSCNPYLALAVLLEAGLRGIEEGILPPKPVGENIFKLNQTERKKRKIQNLPGNLNEALEYFSRSS
LIKDVLGDHIYKKFLETKKKEWKEFMMHVTDWEKEKYLELY
>Mature_441_residues
MDKKSVLELVKRENVRFVRLQITDINGFLKNVEIPVKLLEKSLDEGVMFDGSSIDGFVRIEESDMYLIPDASTVALLPWK
DDDEKTMRIICDVHLPNGEPFEGDPRYRLKKVVQRAKEMGFIAHAGPEPEFFLFKRDENSDRPIVGFMDRGSYFDMLPVD
KGEETRKEIVISLEKMGFDIEAAHHEVAPSQHEIDFRYADILKTADNIQTFKWVVKTIALMNGLYATFMPKPFAQVNGSG
MHIHLSLFENGKNAFYDPEKPYELSDVLLNFVGGIIKYAKELTIVTNPIINSYKRLVPGYEAPINISWALGNRSAMIRVP
AARGQATRIEIRNPDPSCNPYLALAVLLEAGLRGIEEGILPPKPVGENIFKLNQTERKKRKIQNLPGNLNEALEYFSRSS
LIKDVLGDHIYKKFLETKKKEWKEFMMHVTDWEKEKYLELY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=458, Percent_Identity=23.5807860262009, Blast_Score=122, Evalue=9e-28,
Organism=Escherichia coli, GI1790301, Length=428, Percent_Identity=40.4205607476636, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI87081870, Length=448, Percent_Identity=27.4553571428571, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17540008, Length=321, Percent_Identity=24.2990654205607, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50592; Mature: 50592

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKSVLELVKRENVRFVRLQITDINGFLKNVEIPVKLLEKSLDEGVMFDGSSIDGFVRI
CCHHHHHHHHHHCCCEEEEEEEEECCHHHHCCCCHHHHHHHHHCCCEEECCCCCCEEEEE
EESDMYLIPDASTVALLPWKDDDEKTMRIICDVHLPNGEPFEGDPRYRLKKVVQRAKEMG
ECCCEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
FIAHAGPEPEFFLFKRDENSDRPIVGFMDRGSYFDMLPVDKGEETRKEIVISLEKMGFDI
EEEECCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCH
EAAHHEVAPSQHEIDFRYADILKTADNIQTFKWVVKTIALMNGLYATFMPKPFAQVNGSG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
MHIHLSLFENGKNAFYDPEKPYELSDVLLNFVGGIIKYAKELTIVTNPIINSYKRLVPGY
EEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCCC
EAPINISWALGNRSAMIRVPAARGQATRIEIRNPDPSCNPYLALAVLLEAGLRGIEEGIL
CCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
PPKPVGENIFKLNQTERKKRKIQNLPGNLNEALEYFSRSSLIKDVLGDHIYKKFLETKKK
CCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EWKEFMMHVTDWEKEKYLELY
HHHHHHHHHHCCCCCHHEECC
>Mature Secondary Structure
MDKKSVLELVKRENVRFVRLQITDINGFLKNVEIPVKLLEKSLDEGVMFDGSSIDGFVRI
CCHHHHHHHHHHCCCEEEEEEEEECCHHHHCCCCHHHHHHHHHCCCEEECCCCCCEEEEE
EESDMYLIPDASTVALLPWKDDDEKTMRIICDVHLPNGEPFEGDPRYRLKKVVQRAKEMG
ECCCEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
FIAHAGPEPEFFLFKRDENSDRPIVGFMDRGSYFDMLPVDKGEETRKEIVISLEKMGFDI
EEEECCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCH
EAAHHEVAPSQHEIDFRYADILKTADNIQTFKWVVKTIALMNGLYATFMPKPFAQVNGSG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
MHIHLSLFENGKNAFYDPEKPYELSDVLLNFVGGIIKYAKELTIVTNPIINSYKRLVPGY
EEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCCC
EAPINISWALGNRSAMIRVPAARGQATRIEIRNPDPSCNPYLALAVLLEAGLRGIEEGIL
CCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
PPKPVGENIFKLNQTERKKRKIQNLPGNLNEALEYFSRSSLIKDVLGDHIYKKFLETKKK
CCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EWKEFMMHVTDWEKEKYLELY
HHHHHHHHHHCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1348781; 10360571 [H]