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The map label for this gene is mrdA [H]

Identifier: 239616673

GI number: 239616673

Start: 291668

End: 293320

Strand: Reverse

Name: mrdA [H]

Synonym: Kole_0266

Alternate gene names: 239616673

Gene position: 293320-291668 (Counterclockwise)

Preceding gene: 239616674

Following gene: 239616665

Centisome position: 12.74

GC content: 43.86

Gene sequence:

>1653_bases
TTGAATTCGAAAAGAATTGATTTACTATTTCTCTTCTTTCTCCTCGGGGCAGGACTTTTTATATTCAGGCTTTTTCAGTT
TCAAATCCTGGAGCACAAAGAATTCCAGGCAGAAGTGGAAAAAATAATAACACGCGTTTCTTCAATTGAAGCTCCAAGAG
GAAGAATATTCGACAGGAACGGGATCCCCTTAGCATGGAATGAACCGATTTATATTCTAAAGAAAAAGGTGCCTTTCCTT
TCTGAGAAAATCAGGAGCATTCTTTATACGGCACTCAATAGAGCTGATGATGCCACTTCAACCATCAGAAAACTGGAGGC
CTTTGGGGAGGTAGAAGCTCATTTAAGGGCTGATTATATACATCTTCTGGAAGATTCGGACGAAATTGATGTCGAAGAAC
ACTTCGTGCGGCGTTATAGCGAAAATCCAGGAATAGCACATGTCGTAGGCTACATTACATCTGAAGAACAACCGGTTCGG
GGGATTGAAAAATTTTACAACGACCTGTTAACCGGGAAAAATGGCCTGAAGCTCTTGAAAGTGGATAACCACGGAAAGGT
TGTTTCTGTACTGGAAAAAACAGAACCAATTCCCGGGGAAGATCTCGTTCTCACCATAGATGCGAGTCTCAGCAGTTACG
CTTATTCTTTACTGAAATCGTACAACCGTCCCGGCGCAGTTATTGTTATGAAAACCGACGGCCAGATTCTGGCAATGGCT
TCCAATCCCTCGTATTCTCCAGAACAATTCTCGTTAGGCCTTTCCGATAGGGAATGGACAAGACTCAAATACGATCCTGA
AGGCCCTCTGCTTAACAGAGCTGTTTCCCCGTATCCGCCTGGTTCAATCATAAAACCTTTTATTTCTATGGTGGCTCTTT
CAGAAAAAGTTTCGCCAGACGCAACCATCTTTTGTTCGGGGATTTTTCAGTACAAAGATTCAGAGGGACATACCCTGGCT
GTGTTTTCTGATTGGTATCTTTACGGTCATAAAGAAGTTGATTTGAGAAGAGCCCTTGCGGTCTCCTGCAACGTGTATTT
TTATGAGTTAGGGCTTAAACTTGGTATAGACACGCTAACCCATTATGCTGAACTGACGGGCATTTTTGGCAAAACAGGTA
TAGATCTTGAGGAAAGAGAGGGACTCATGCCATCAAGGGAATGGAAATTTAAAACCTACGGTGAGCCGTGGTATCCCGGT
GATACGATCCTAACCTACATCGGTCAGGGGTATGCGCTTATGACACCTCTTGAGGTAACAAGAATGACGGCGATAATAGC
TACAAAAGGGTGTGAGGTTGTCCCATATATATATCGAAGGAGTGCAACGAACGAAAAAGACTTTCATCTTTCCGAAGATG
CGTGGGAGACAATCATAGCTGGAATGAAAGATGTCATATCACGTGCCGGAACAGGTCCTGCTGATAGAGGAACGGCCTAC
CATGCTTTCAAAGGGTTTCCTTACGATGTTGCTGGAAAAACCGGAACGGCAGAAAATACTGGTAATGTTCCGCATTCCTG
GTTTACAGGCTTTTCTCCGGTTGATGATCCAGAAATCATAGTAACGGTGTTTGTCGAAAACGGCGGCTATGGTTCAACAA
CAGCCGCCGCGATAGCAAGAAAGATCTTTGAAGCATACTACGGGATTCGTTGA

Upstream 100 bases:

>100_bases
ACATTTCTGCTGTCGCTATCGACATTGGTATTCAATACCTTCTTTCCTGGAATTTTTGTAGCTCCGCTGCTGATTCTATT
TATTTTGAGGAGGAAAATCC

Downstream 100 bases:

>100_bases
TTGATTAACCTCGATTCCTTTCATATCTTCAAGAGACTTATTCGTAATTGGCAAATCGGCCACCAAGCATTGCTATTGCC
AGTATCATCCTGAATATTTC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MNSKRIDLLFLFFLLGAGLFIFRLFQFQILEHKEFQAEVEKIITRVSSIEAPRGRIFDRNGIPLAWNEPIYILKKKVPFL
SEKIRSILYTALNRADDATSTIRKLEAFGEVEAHLRADYIHLLEDSDEIDVEEHFVRRYSENPGIAHVVGYITSEEQPVR
GIEKFYNDLLTGKNGLKLLKVDNHGKVVSVLEKTEPIPGEDLVLTIDASLSSYAYSLLKSYNRPGAVIVMKTDGQILAMA
SNPSYSPEQFSLGLSDREWTRLKYDPEGPLLNRAVSPYPPGSIIKPFISMVALSEKVSPDATIFCSGIFQYKDSEGHTLA
VFSDWYLYGHKEVDLRRALAVSCNVYFYELGLKLGIDTLTHYAELTGIFGKTGIDLEEREGLMPSREWKFKTYGEPWYPG
DTILTYIGQGYALMTPLEVTRMTAIIATKGCEVVPYIYRRSATNEKDFHLSEDAWETIIAGMKDVISRAGTGPADRGTAY
HAFKGFPYDVAGKTGTAENTGNVPHSWFTGFSPVDDPEIIVTVFVENGGYGSTTAAAIARKIFEAYYGIR

Sequences:

>Translated_550_residues
MNSKRIDLLFLFFLLGAGLFIFRLFQFQILEHKEFQAEVEKIITRVSSIEAPRGRIFDRNGIPLAWNEPIYILKKKVPFL
SEKIRSILYTALNRADDATSTIRKLEAFGEVEAHLRADYIHLLEDSDEIDVEEHFVRRYSENPGIAHVVGYITSEEQPVR
GIEKFYNDLLTGKNGLKLLKVDNHGKVVSVLEKTEPIPGEDLVLTIDASLSSYAYSLLKSYNRPGAVIVMKTDGQILAMA
SNPSYSPEQFSLGLSDREWTRLKYDPEGPLLNRAVSPYPPGSIIKPFISMVALSEKVSPDATIFCSGIFQYKDSEGHTLA
VFSDWYLYGHKEVDLRRALAVSCNVYFYELGLKLGIDTLTHYAELTGIFGKTGIDLEEREGLMPSREWKFKTYGEPWYPG
DTILTYIGQGYALMTPLEVTRMTAIIATKGCEVVPYIYRRSATNEKDFHLSEDAWETIIAGMKDVISRAGTGPADRGTAY
HAFKGFPYDVAGKTGTAENTGNVPHSWFTGFSPVDDPEIIVTVFVENGGYGSTTAAAIARKIFEAYYGIR
>Mature_550_residues
MNSKRIDLLFLFFLLGAGLFIFRLFQFQILEHKEFQAEVEKIITRVSSIEAPRGRIFDRNGIPLAWNEPIYILKKKVPFL
SEKIRSILYTALNRADDATSTIRKLEAFGEVEAHLRADYIHLLEDSDEIDVEEHFVRRYSENPGIAHVVGYITSEEQPVR
GIEKFYNDLLTGKNGLKLLKVDNHGKVVSVLEKTEPIPGEDLVLTIDASLSSYAYSLLKSYNRPGAVIVMKTDGQILAMA
SNPSYSPEQFSLGLSDREWTRLKYDPEGPLLNRAVSPYPPGSIIKPFISMVALSEKVSPDATIFCSGIFQYKDSEGHTLA
VFSDWYLYGHKEVDLRRALAVSCNVYFYELGLKLGIDTLTHYAELTGIFGKTGIDLEEREGLMPSREWKFKTYGEPWYPG
DTILTYIGQGYALMTPLEVTRMTAIIATKGCEVVPYIYRRSATNEKDFHLSEDAWETIIAGMKDVISRAGTGPADRGTAY
HAFKGFPYDVAGKTGTAENTGNVPHSWFTGFSPVDDPEIIVTVFVENGGYGSTTAAAIARKIFEAYYGIR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=607, Percent_Identity=30.3130148270181, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1786272, Length=582, Percent_Identity=25.9450171821306, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 61707; Mature: 61707

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKRIDLLFLFFLLGAGLFIFRLFQFQILEHKEFQAEVEKIITRVSSIEAPRGRIFDRN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
GIPLAWNEPIYILKKKVPFLSEKIRSILYTALNRADDATSTIRKLEAFGEVEAHLRADYI
CCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HLLEDSDEIDVEEHFVRRYSENPGIAHVVGYITSEEQPVRGIEKFYNDLLTGKNGLKLLK
EEECCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEE
VDNHGKVVSVLEKTEPIPGEDLVLTIDASLSSYAYSLLKSYNRPGAVIVMKTDGQILAMA
ECCCCCEEEHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEE
SNPSYSPEQFSLGLSDREWTRLKYDPEGPLLNRAVSPYPPGSIIKPFISMVALSEKVSPD
CCCCCCCHHEEECCCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
ATIFCSGIFQYKDSEGHTLAVFSDWYLYGHKEVDLRRALAVSCNVYFYELGLKLGIDTLT
CEEEEEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHCCEEEEEEECHHHHHHHHH
HYAELTGIFGKTGIDLEEREGLMPSREWKFKTYGEPWYPGDTILTYIGQGYALMTPLEVT
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHCCCEEEECCHHHH
RMTAIIATKGCEVVPYIYRRSATNEKDFHLSEDAWETIIAGMKDVISRAGTGPADRGTAY
HHHHHEEECCCCEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
HAFKGFPYDVAGKTGTAENTGNVPHSWFTGFSPVDDPEIIVTVFVENGGYGSTTAAAIAR
EHHCCCCEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
KIFEAYYGIR
HHHHHHHCCC
>Mature Secondary Structure
MNSKRIDLLFLFFLLGAGLFIFRLFQFQILEHKEFQAEVEKIITRVSSIEAPRGRIFDRN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
GIPLAWNEPIYILKKKVPFLSEKIRSILYTALNRADDATSTIRKLEAFGEVEAHLRADYI
CCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HLLEDSDEIDVEEHFVRRYSENPGIAHVVGYITSEEQPVRGIEKFYNDLLTGKNGLKLLK
EEECCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEE
VDNHGKVVSVLEKTEPIPGEDLVLTIDASLSSYAYSLLKSYNRPGAVIVMKTDGQILAMA
ECCCCCEEEHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEE
SNPSYSPEQFSLGLSDREWTRLKYDPEGPLLNRAVSPYPPGSIIKPFISMVALSEKVSPD
CCCCCCCHHEEECCCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
ATIFCSGIFQYKDSEGHTLAVFSDWYLYGHKEVDLRRALAVSCNVYFYELGLKLGIDTLT
CEEEEEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHCCEEEEEEECHHHHHHHHH
HYAELTGIFGKTGIDLEEREGLMPSREWKFKTYGEPWYPGDTILTYIGQGYALMTPLEVT
HHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHCCCEEEECCHHHH
RMTAIIATKGCEVVPYIYRRSATNEKDFHLSEDAWETIIAGMKDVISRAGTGPADRGTAY
HHHHHEEECCCCEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
HAFKGFPYDVAGKTGTAENTGNVPHSWFTGFSPVDDPEIIVTVFVENGGYGSTTAAAIAR
EHHCCCCEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
KIFEAYYGIR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]