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The map label for this gene is polA [H]

Identifier: 239616633

GI number: 239616633

Start: 253586

End: 256276

Strand: Reverse

Name: polA [H]

Synonym: Kole_0226

Alternate gene names: 239616633

Gene position: 256276-253586 (Counterclockwise)

Preceding gene: 239616634

Following gene: 239616632

Centisome position: 11.13

GC content: 44.78

Gene sequence:

>2691_bases
ATGGCTAACAGGCTTTTTCTTTTTGACGGAACGGCCATTGTTTACAGGGCATACTTCGCGATAGATGTTTCTCTGACAAA
CTCCAAAGGAGAACCCACCAACGCGGTTTACGGCACCGCGAGAATGCTTTCCAGATTCATCAAAAACTACATTGCTGAAG
GAGACTACGCACTATTTGCCTTTGATCGCAAAGAAGCCACACACCGCCACGATCTCTTCGAAGGATATAAAGCGACTCGC
GCAGAGATGCCGGATGCCCTCGTAGCACAACTCAAATATATTCCAGACCTTGTAGAAGGCTTTGGAATAAAATTTCATTC
CGTGGCCACCCTTGAAGCCGACGATATAATTGCCACTGCCGTTACGAAATACAGGGATAAGTTCGATGAGATAGTTATAG
TATCTGGCGATAAGGATATCCTCCAGCTCGTGGATGAAAAAGTCAAAGTTCTGCGCTTTGTTTCGGGGCTTACCGATCTG
GAGGAATACGACAGGAGGAAGGTTGAAGAAAAGTTTGGGCTTCCGCCGGAAAAGATATACGAACTGCTATCACTTTGTGG
TGATACCTCTGACAATATTCCCGGTGTTCCGGGTATTGGAATAAAAACGGCATTGAAGTTATTGAAAGAGTACGGCTCCA
TTGATGAGATATACAAGAATATCCGTAAACTGAGCCCCGGGCTCAGAAAGAAGCTGATGGACGGCAAAAAAGCGCTGGAG
ATGTCGAAAAAGCTCGTTCGCCTGGTTACGGATGCGGATTTACACATAGAACTTGAAGAGCTCAGATACAGAGGATTGGA
CAAAGAAAAACTCAGAGAACTTTTCCTGAAGCTGGAATTTGCCTCGCTTTTAAGAGAATTCGATCTTACCAACTTGGGCT
CTGATAATAACGAAGACAATGGTTACAGGCCGGTAAGCTCCCAGCAAGAGCTCGATGAGCTCTTTGAAACCCTCAAAGGT
AGCGATGTGATTTCGATTGATCTCGAAACCAGCTCCCTTGACCCTCACGAAGCGAAGATTGTCGGTATCTCTGTAGCGAT
AAAGGATGGAGAGGGTTTTTATATTCCTGTTAATCACGAAAGCAGCGATTGGCAGGCAGACGAAAAACAGGTTCTTCTGA
GACTCAAAGAACTTCTGGAAGATTCCGGCACGAAGATCGTCGGCCAGAACCTAAAATTCGATTACGAAATACTCGAAAAG
CATGGTATAGAACCAGTTGTGCCTCATTTCGATACAATGATTGCAGCTTATCTTTTGAACCCGGATTCAAGACGTTTCAA
CCTTGATGACCTGGCTTTGAAGTTCCTGGGATATCGCACCACTTCTTTTAGCGAGCTGATGAATAAGAATCAATTGAAAG
ATCAGTTTGAAAAAGTTTCCGTTGAGGAAGCTGCAAAATACTCTGTCGAAGACGCCGATATAGCGCTCAGGCTCTATCGG
GTGCTTTCTAAGAAATTATATGAATCTGATCTCGACAACATTTTCCATAAAATCGAGATGGAACTCATACCCGTTTTGGC
GGAACTCGAACTCAACGGCGTGTACATGAACGTGGAATCTTTGAAAAATCTTTCAGAAGAATACGAGAAGAGACTCGGGA
AGATAAGAGAAGAACTCTTTGAACTTGCTGGTGAGCCTTTTAATCCAAATTCTCCCACTCAGGTTTCAAAGATACTTTTT
GAGAAACTCGGATTGAATCCGCCGAAAAAGACCAAGCATGGCGCTTATTCTACGAGCGCAGCTGTTCTAGAAGAGCTTGT
GAATGAGCATCCTATTATTCAGAAACTCCTGGATTACAGGAAGTACCAGAAACTCAAATCCACGTATCTCGATACGCTTC
CGACCCTTGTAAACCCGGTTACGGGAAGGATTCACGCCAGTTATCACCAGACGGGAACCGGTACAGGCCGTCTTTCAAGC
AGTAATCCCAACATGCAGAATTTACCGATAAAGGGTGAAGAGGGCAAGGAAATCAGAAAATGTGTTGTCCCTCAGAAGAG
TGATTGGAAGATCATAAGTGCCGATTATTCCCAGATTGAACTCAGGGTACTGGCGCACTTCAGCGAGGACGAAAGATTGA
TTTCGGCATTCAAAAACGGCGAAGACGTGCATGCCCTTACCGCGTCACGTTTGTACGGAGTTTCTGTTAAAGATGTGACA
CCGGAGATGAGACAGGTCGGAAAGATGGTTAACTTTTCGATTATTTACGGGATATCTCCTTACGGTCTTGCAAGAAGACT
CAAGATAAAAACCCACATAGCTGAGAATATGATATCGAATTATTTCAACGCTTATCCGGGGGTCAGGAAGTTCATAAATG
AAGTTATACACGAAGCCAAAGAAAAGGGATACGTGAGGACGTTGTTCGGTAGAAAAAGGGAGATACCTCACTTCAGGACG
CGTAACAAGATGAAGATCCAGGAAGGGGAAAGAATTGCGATCAACACCCCGATTCAGGGAACGGCGGCGGATATCATGAA
GCTTGCCATGATCAAGATACATAAGATGGTTAAGGAAGAGGGATTGGAAGCTTTCCCAATTTTGCAGGTGCACGATGAAC
TGGTTTTTGAAGCTCCCGCAACAGAGGTTGACAGAGTTTGTGATATACTCACCAGGGGTATGTCCGGGGTCGTTGAACTG
AAGGTTCCGCTTGAGGTAGATGTGGCAGTTAACAGTTACTGGGGTAAATAA

Upstream 100 bases:

>100_bases
AAGCCTTTAACGTCGCGCTTGTAGTGGGAGGTTCGATAGTTATACCCCATTTCAGACGCGCGGTTGCTCTCCTCGATGAA
CTGGAAGGAGAGGATTGAAA

Downstream 100 bases:

>100_bases
CTCCAGAGGTGGGCGTGGTGAAAAAGAAAAGGATCGATTTCAGACAATTAAACGCTATAAACATGGTATTTCAATTTGGA
ATCGTTGTAATCTCCAACAT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 896; Mature: 895

Protein sequence:

>896_residues
MANRLFLFDGTAIVYRAYFAIDVSLTNSKGEPTNAVYGTARMLSRFIKNYIAEGDYALFAFDRKEATHRHDLFEGYKATR
AEMPDALVAQLKYIPDLVEGFGIKFHSVATLEADDIIATAVTKYRDKFDEIVIVSGDKDILQLVDEKVKVLRFVSGLTDL
EEYDRRKVEEKFGLPPEKIYELLSLCGDTSDNIPGVPGIGIKTALKLLKEYGSIDEIYKNIRKLSPGLRKKLMDGKKALE
MSKKLVRLVTDADLHIELEELRYRGLDKEKLRELFLKLEFASLLREFDLTNLGSDNNEDNGYRPVSSQQELDELFETLKG
SDVISIDLETSSLDPHEAKIVGISVAIKDGEGFYIPVNHESSDWQADEKQVLLRLKELLEDSGTKIVGQNLKFDYEILEK
HGIEPVVPHFDTMIAAYLLNPDSRRFNLDDLALKFLGYRTTSFSELMNKNQLKDQFEKVSVEEAAKYSVEDADIALRLYR
VLSKKLYESDLDNIFHKIEMELIPVLAELELNGVYMNVESLKNLSEEYEKRLGKIREELFELAGEPFNPNSPTQVSKILF
EKLGLNPPKKTKHGAYSTSAAVLEELVNEHPIIQKLLDYRKYQKLKSTYLDTLPTLVNPVTGRIHASYHQTGTGTGRLSS
SNPNMQNLPIKGEEGKEIRKCVVPQKSDWKIISADYSQIELRVLAHFSEDERLISAFKNGEDVHALTASRLYGVSVKDVT
PEMRQVGKMVNFSIIYGISPYGLARRLKIKTHIAENMISNYFNAYPGVRKFINEVIHEAKEKGYVRTLFGRKREIPHFRT
RNKMKIQEGERIAINTPIQGTAADIMKLAMIKIHKMVKEEGLEAFPILQVHDELVFEAPATEVDRVCDILTRGMSGVVEL
KVPLEVDVAVNSYWGK

Sequences:

>Translated_896_residues
MANRLFLFDGTAIVYRAYFAIDVSLTNSKGEPTNAVYGTARMLSRFIKNYIAEGDYALFAFDRKEATHRHDLFEGYKATR
AEMPDALVAQLKYIPDLVEGFGIKFHSVATLEADDIIATAVTKYRDKFDEIVIVSGDKDILQLVDEKVKVLRFVSGLTDL
EEYDRRKVEEKFGLPPEKIYELLSLCGDTSDNIPGVPGIGIKTALKLLKEYGSIDEIYKNIRKLSPGLRKKLMDGKKALE
MSKKLVRLVTDADLHIELEELRYRGLDKEKLRELFLKLEFASLLREFDLTNLGSDNNEDNGYRPVSSQQELDELFETLKG
SDVISIDLETSSLDPHEAKIVGISVAIKDGEGFYIPVNHESSDWQADEKQVLLRLKELLEDSGTKIVGQNLKFDYEILEK
HGIEPVVPHFDTMIAAYLLNPDSRRFNLDDLALKFLGYRTTSFSELMNKNQLKDQFEKVSVEEAAKYSVEDADIALRLYR
VLSKKLYESDLDNIFHKIEMELIPVLAELELNGVYMNVESLKNLSEEYEKRLGKIREELFELAGEPFNPNSPTQVSKILF
EKLGLNPPKKTKHGAYSTSAAVLEELVNEHPIIQKLLDYRKYQKLKSTYLDTLPTLVNPVTGRIHASYHQTGTGTGRLSS
SNPNMQNLPIKGEEGKEIRKCVVPQKSDWKIISADYSQIELRVLAHFSEDERLISAFKNGEDVHALTASRLYGVSVKDVT
PEMRQVGKMVNFSIIYGISPYGLARRLKIKTHIAENMISNYFNAYPGVRKFINEVIHEAKEKGYVRTLFGRKREIPHFRT
RNKMKIQEGERIAINTPIQGTAADIMKLAMIKIHKMVKEEGLEAFPILQVHDELVFEAPATEVDRVCDILTRGMSGVVEL
KVPLEVDVAVNSYWGK
>Mature_895_residues
ANRLFLFDGTAIVYRAYFAIDVSLTNSKGEPTNAVYGTARMLSRFIKNYIAEGDYALFAFDRKEATHRHDLFEGYKATRA
EMPDALVAQLKYIPDLVEGFGIKFHSVATLEADDIIATAVTKYRDKFDEIVIVSGDKDILQLVDEKVKVLRFVSGLTDLE
EYDRRKVEEKFGLPPEKIYELLSLCGDTSDNIPGVPGIGIKTALKLLKEYGSIDEIYKNIRKLSPGLRKKLMDGKKALEM
SKKLVRLVTDADLHIELEELRYRGLDKEKLRELFLKLEFASLLREFDLTNLGSDNNEDNGYRPVSSQQELDELFETLKGS
DVISIDLETSSLDPHEAKIVGISVAIKDGEGFYIPVNHESSDWQADEKQVLLRLKELLEDSGTKIVGQNLKFDYEILEKH
GIEPVVPHFDTMIAAYLLNPDSRRFNLDDLALKFLGYRTTSFSELMNKNQLKDQFEKVSVEEAAKYSVEDADIALRLYRV
LSKKLYESDLDNIFHKIEMELIPVLAELELNGVYMNVESLKNLSEEYEKRLGKIREELFELAGEPFNPNSPTQVSKILFE
KLGLNPPKKTKHGAYSTSAAVLEELVNEHPIIQKLLDYRKYQKLKSTYLDTLPTLVNPVTGRIHASYHQTGTGTGRLSSS
NPNMQNLPIKGEEGKEIRKCVVPQKSDWKIISADYSQIELRVLAHFSEDERLISAFKNGEDVHALTASRLYGVSVKDVTP
EMRQVGKMVNFSIIYGISPYGLARRLKIKTHIAENMISNYFNAYPGVRKFINEVIHEAKEKGYVRTLFGRKREIPHFRTR
NKMKIQEGERIAINTPIQGTAADIMKLAMIKIHKMVKEEGLEAFPILQVHDELVFEAPATEVDRVCDILTRGMSGVVELK
VPLEVDVAVNSYWGK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=518, Percent_Identity=31.4671814671815, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI139394648, Length=599, Percent_Identity=25.8764607679466, Blast_Score=191, Evalue=4e-48,
Organism=Escherichia coli, GI1790294, Length=933, Percent_Identity=40.3001071811361, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI226510970, Length=206, Percent_Identity=30.0970873786408, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI86563272, Length=448, Percent_Identity=28.5714285714286, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI17933644, Length=428, Percent_Identity=31.5420560747664, Blast_Score=197, Evalue=3e-50,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 101820; Mature: 101689

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLFLFDGTAIVYRAYFAIDVSLTNSKGEPTNAVYGTARMLSRFIKNYIAEGDYALFA
CCCEEEEECCHHHHEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
FDRKEATHRHDLFEGYKATRAEMPDALVAQLKYIPDLVEGFGIKFHSVATLEADDIIATA
ECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHH
VTKYRDKFDEIVIVSGDKDILQLVDEKVKVLRFVSGLTDLEEYDRRKVEEKFGLPPEKIY
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELLSLCGDTSDNIPGVPGIGIKTALKLLKEYGSIDEIYKNIRKLSPGLRKKLMDGKKALE
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHCHHHHHH
MSKKLVRLVTDADLHIELEELRYRGLDKEKLRELFLKLEFASLLREFDLTNLGSDNNEDN
HHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GYRPVSSQQELDELFETLKGSDVISIDLETSSLDPHEAKIVGISVAIKDGEGFYIPVNHE
CCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCEEEEECCCC
SSDWQADEKQVLLRLKELLEDSGTKIVGQNLKFDYEILEKHGIEPVVPHFDTMIAAYLLN
CCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCC
PDSRRFNLDDLALKFLGYRTTSFSELMNKNQLKDQFEKVSVEEAAKYSVEDADIALRLYR
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHH
VLSKKLYESDLDNIFHKIEMELIPVLAELELNGVYMNVESLKNLSEEYEKRLGKIREELF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ELAGEPFNPNSPTQVSKILFEKLGLNPPKKTKHGAYSTSAAVLEELVNEHPIIQKLLDYR
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
KYQKLKSTYLDTLPTLVNPVTGRIHASYHQTGTGTGRLSSSNPNMQNLPIKGEEGKEIRK
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHH
CVVPQKSDWKIISADYSQIELRVLAHFSEDERLISAFKNGEDVHALTASRLYGVSVKDVT
HCCCCCCCCEEEECCHHHEEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCC
PEMRQVGKMVNFSIIYGISPYGLARRLKIKTHIAENMISNYFNAYPGVRKFINEVIHEAK
HHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EKGYVRTLFGRKREIPHFRTRNKMKIQEGERIAINTPIQGTAADIMKLAMIKIHKMVKEE
HCCCHHHHHCCCCCCCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
GLEAFPILQVHDELVFEAPATEVDRVCDILTRGMSGVVELKVPLEVDVAVNSYWGK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCCCCC
>Mature Secondary Structure 
ANRLFLFDGTAIVYRAYFAIDVSLTNSKGEPTNAVYGTARMLSRFIKNYIAEGDYALFA
CCEEEEECCHHHHEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
FDRKEATHRHDLFEGYKATRAEMPDALVAQLKYIPDLVEGFGIKFHSVATLEADDIIATA
ECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHH
VTKYRDKFDEIVIVSGDKDILQLVDEKVKVLRFVSGLTDLEEYDRRKVEEKFGLPPEKIY
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELLSLCGDTSDNIPGVPGIGIKTALKLLKEYGSIDEIYKNIRKLSPGLRKKLMDGKKALE
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHCHHHHHH
MSKKLVRLVTDADLHIELEELRYRGLDKEKLRELFLKLEFASLLREFDLTNLGSDNNEDN
HHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GYRPVSSQQELDELFETLKGSDVISIDLETSSLDPHEAKIVGISVAIKDGEGFYIPVNHE
CCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCEEEEECCCC
SSDWQADEKQVLLRLKELLEDSGTKIVGQNLKFDYEILEKHGIEPVVPHFDTMIAAYLLN
CCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCC
PDSRRFNLDDLALKFLGYRTTSFSELMNKNQLKDQFEKVSVEEAAKYSVEDADIALRLYR
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHH
VLSKKLYESDLDNIFHKIEMELIPVLAELELNGVYMNVESLKNLSEEYEKRLGKIREELF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ELAGEPFNPNSPTQVSKILFEKLGLNPPKKTKHGAYSTSAAVLEELVNEHPIIQKLLDYR
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
KYQKLKSTYLDTLPTLVNPVTGRIHASYHQTGTGTGRLSSSNPNMQNLPIKGEEGKEIRK
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHH
CVVPQKSDWKIISADYSQIELRVLAHFSEDERLISAFKNGEDVHALTASRLYGVSVKDVT
HCCCCCCCCEEEECCHHHEEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCC
PEMRQVGKMVNFSIIYGISPYGLARRLKIKTHIAENMISNYFNAYPGVRKFINEVIHEAK
HHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EKGYVRTLFGRKREIPHFRTRNKMKIQEGERIAINTPIQGTAADIMKLAMIKIHKMVKEE
HCCCHHHHHCCCCCCCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
GLEAFPILQVHDELVFEAPATEVDRVCDILTRGMSGVVELKVPLEVDVAVNSYWGK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]