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The map label for this gene is ahpF [H]

Identifier: 239616566

GI number: 239616566

Start: 174135

End: 175373

Strand: Reverse

Name: ahpF [H]

Synonym: Kole_0158

Alternate gene names: 239616566

Gene position: 175373-174135 (Counterclockwise)

Preceding gene: 239616567

Following gene: 239616565

Centisome position: 7.62

GC content: 47.46

Gene sequence:

>1239_bases
ATGAAGAAACTCAAAACAGATGTACTCGTGATAGGCGGTGGAGCGGCCGGGATGGCTGCGGCCATTGGAGCTGCGAGAGA
GGGTTCGAAGGTGATTCTAATTGAACGTGAAGATCGTACCGGCGGGGTTCTTAACCAGTGTATTCACAATGGCTTCGGTT
TGAAGTTTTACGAAGAAGAGCTGACCGGACCCGAATTCGCAGCACGGTTGGAAGAAGAAATGAGTCAGAAAAGTATTGAT
ATCATTTCTCAAGCTTATTTGAGGGAAGTGGATTTAGATAACAAAATTGCATATATCCTTTCTCCGGAAGATGCTTTCAT
TGTCAAGTCGAAAGCGATTGTGATATCTACAGGTGCGAGGGAACGTCCTTTTGGCTCTCTTATGGTTCCCGGAGATAGGC
CAGCAGGGATCTTTACTGCCGGCACAGCCCAGAGGTTCGTCAATCTGGAAAACAGGCTTCCGGGAAAAAGGGCTCTCGTT
CTCGGTTCAGGGGATATAGGGCTCATAATGGCAAGAAGGCTCTTTCTTGAAGGAATGGAAGTGGTAGGGGTTGTAGAAAG
ACTTCCATATCCTGGAGGCCTCACGAGAAACGTGGTCCAGTGCCTTGAAGATTTCGGAATTCCTCTCTATCTATCAGCGA
CTGTAACGTATGTAAAAGGATTTCCGCGTTTAGAGGCTGTAGAGGTGATGGAGGTTGACAAAAACCTCAATCCAATCCCG
GGCACAGAGAAATGGTACAACGTGGATACGCTGGTTCTCTCTGCTGGTTTGCTTCCGCAGGTGGAAGATTTCTCCGACAG
GTTGCTGGTTGACCGTATAAACAGGGGATTTCTCGTTTCCAATACCTGTGAAACCTCCAAGCCAGGGATCTTTGCTGCGG
GAAACAACGTAGCCGTTTTCGATCTCGTCGATTATGTTGCGGCCGAGGGCTGGATAGCTGGAAGGCACGCAGCACTTACC
GCTCAAGAAAAGCGTCAAAATCACAAACAGTTTCCTGTTGTGAGGGGTAAAAACGTAGGAGTTCTTGTGCCAGGGAAAAT
ATCACTTGAGGAAAATCTGAGGATTTATCTCAGGTTGAAAAAACCAATGGAGTCGGGTACGGTGGTCTTAAAAGAACTCG
GGATTGAATCCTCTTTCAAATACGGAGTCCCAAGTGAGATGCTCCAGATTGTCGTGAACAAGAATAAACTCGCCGCGATT
AAGAATCACGAGAAGCTTACCGTGGAGGTGATTGAATGA

Upstream 100 bases:

>100_bases
GGAAGATTGCAAGAATACTTGCGAGAGAGTTGAATATCGATATATCGGAGGTTCTGCAGAATGAGGATGAAAGTTGGCTG
GTTAGAGAGAAGGTGAGGCC

Downstream 100 bases:

>100_bases
AGGGACATATAACCTGCGTTTTATGCCCTCTTGGTTGTCGTATCTCTATATCTGAAAAAAACGGTGAGTACAAAATTGAA
GGGAACAGATGTCCGCGTGG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: Alkyl hydroperoxide reductase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKKLKTDVLVIGGGAAGMAAAIGAAREGSKVILIEREDRTGGVLNQCIHNGFGLKFYEEELTGPEFAARLEEEMSQKSID
IISQAYLREVDLDNKIAYILSPEDAFIVKSKAIVISTGARERPFGSLMVPGDRPAGIFTAGTAQRFVNLENRLPGKRALV
LGSGDIGLIMARRLFLEGMEVVGVVERLPYPGGLTRNVVQCLEDFGIPLYLSATVTYVKGFPRLEAVEVMEVDKNLNPIP
GTEKWYNVDTLVLSAGLLPQVEDFSDRLLVDRINRGFLVSNTCETSKPGIFAAGNNVAVFDLVDYVAAEGWIAGRHAALT
AQEKRQNHKQFPVVRGKNVGVLVPGKISLEENLRIYLRLKKPMESGTVVLKELGIESSFKYGVPSEMLQIVVNKNKLAAI
KNHEKLTVEVIE

Sequences:

>Translated_412_residues
MKKLKTDVLVIGGGAAGMAAAIGAAREGSKVILIEREDRTGGVLNQCIHNGFGLKFYEEELTGPEFAARLEEEMSQKSID
IISQAYLREVDLDNKIAYILSPEDAFIVKSKAIVISTGARERPFGSLMVPGDRPAGIFTAGTAQRFVNLENRLPGKRALV
LGSGDIGLIMARRLFLEGMEVVGVVERLPYPGGLTRNVVQCLEDFGIPLYLSATVTYVKGFPRLEAVEVMEVDKNLNPIP
GTEKWYNVDTLVLSAGLLPQVEDFSDRLLVDRINRGFLVSNTCETSKPGIFAAGNNVAVFDLVDYVAAEGWIAGRHAALT
AQEKRQNHKQFPVVRGKNVGVLVPGKISLEENLRIYLRLKKPMESGTVVLKELGIESSFKYGVPSEMLQIVVNKNKLAAI
KNHEKLTVEVIE
>Mature_412_residues
MKKLKTDVLVIGGGAAGMAAAIGAAREGSKVILIEREDRTGGVLNQCIHNGFGLKFYEEELTGPEFAARLEEEMSQKSID
IISQAYLREVDLDNKIAYILSPEDAFIVKSKAIVISTGARERPFGSLMVPGDRPAGIFTAGTAQRFVNLENRLPGKRALV
LGSGDIGLIMARRLFLEGMEVVGVVERLPYPGGLTRNVVQCLEDFGIPLYLSATVTYVKGFPRLEAVEVMEVDKNLNPIP
GTEKWYNVDTLVLSAGLLPQVEDFSDRLLVDRINRGFLVSNTCETSKPGIFAAGNNVAVFDLVDYVAAEGWIAGRHAALT
AQEKRQNHKQFPVVRGKNVGVLVPGKISLEENLRIYLRLKKPMESGTVVLKELGIESSFKYGVPSEMLQIVVNKNKLAAI
KNHEKLTVEVIE

Specific function: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.6.99.3 [H]

Molecular weight: Translated: 45096; Mature: 45096

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKTDVLVIGGGAAGMAAAIGAAREGSKVILIEREDRTGGVLNQCIHNGFGLKFYEEE
CCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEHHH
LTGPEFAARLEEEMSQKSIDIISQAYLREVDLDNKIAYILSPEDAFIVKSKAIVISTGAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCEEEEECCCC
ERPFGSLMVPGDRPAGIFTAGTAQRFVNLENRLPGKRALVLGSGDIGLIMARRLFLEGME
CCCCCCEECCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCHH
VVGVVERLPYPGGLTRNVVQCLEDFGIPLYLSATVTYVKGFPRLEAVEVMEVDKNLNPIP
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHEECCCCCCCCC
GTEKWYNVDTLVLSAGLLPQVEDFSDRLLVDRINRGFLVSNTCETSKPGIFAAGNNVAVF
CCCCEEEHHHHEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEH
DLVDYVAAEGWIAGRHAALTAQEKRQNHKQFPVVRGKNVGVLVPGKISLEENLRIYLRLK
HHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCCEEECCCCCEEECCEEEECCCCEEEEEEE
KPMESGTVVLKELGIESSFKYGVPSEMLQIVVNKNKLAAIKNHEKLTVEVIE
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEEEEC
>Mature Secondary Structure
MKKLKTDVLVIGGGAAGMAAAIGAAREGSKVILIEREDRTGGVLNQCIHNGFGLKFYEEE
CCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEHHH
LTGPEFAARLEEEMSQKSIDIISQAYLREVDLDNKIAYILSPEDAFIVKSKAIVISTGAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCEEEEECCCC
ERPFGSLMVPGDRPAGIFTAGTAQRFVNLENRLPGKRALVLGSGDIGLIMARRLFLEGME
CCCCCCEECCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCHH
VVGVVERLPYPGGLTRNVVQCLEDFGIPLYLSATVTYVKGFPRLEAVEVMEVDKNLNPIP
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHEECCCCCCCCC
GTEKWYNVDTLVLSAGLLPQVEDFSDRLLVDRINRGFLVSNTCETSKPGIFAAGNNVAVF
CCCCEEEHHHHEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEH
DLVDYVAAEGWIAGRHAALTAQEKRQNHKQFPVVRGKNVGVLVPGKISLEENLRIYLRLK
HHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCCEEECCCCCEEECCEEEECCCCEEEEEEE
KPMESGTVVLKELGIESSFKYGVPSEMLQIVVNKNKLAAIKNHEKLTVEVIE
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1917890; 2760020 [H]