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The map label for this gene is dat [H]

Identifier: 239616495

GI number: 239616495

Start: 99880

End: 100740

Strand: Reverse

Name: dat [H]

Synonym: Kole_0085

Alternate gene names: 239616495

Gene position: 100740-99880 (Counterclockwise)

Preceding gene: 239616496

Following gene: 239616489

Centisome position: 4.38

GC content: 41.93

Gene sequence:

>861_bases
ATGAAAGCATTTTTGAATGGAAAATTTGTTGAAAAAAACGAAAAGCTAATAACTCTTGAAAACAGAGGCCTGACCTTTGG
CGATGGGCTCTTCGAAGTCCTCAGAGTAAAAGATGGGCATATCTTTTTCTTCGAAGACCATCTATTGCGAATGAAAAGCA
GTGCGTCCTTTTTTGGCATTCCCTTTCCCTATTCACTTGATGAAATAAAAAACAGAGCCCACGAATTGATCTCTCTTAAC
CGTATAAATGATGGCGAGCTGTACATAGAACTGACCCGTGGTGTTGATACAAACCGTGAGCATAGATACCCACCGAGAAG
CACACAAACCACATTTTTCATGCTCGCTATTCCCCTGCGTGAGATCAATCCTGAAAACTGGAAAAGGGGTGCCGCGGTCT
TCAGCTTTCCGGATCTACGCCACAAACTCTGTGAGCATAAGACCATAAACCTCCTTCCAAACGTGATGGCAAAGAACTTT
GCCTATGAACGTGGAGGTTATGAAGCACTCATGTACAGAGAGAATTCAAAAGGAAAGTATATAACCGAAGGAGGCAGCTC
AAACTACTTCCTGGTAAAAAATTGTACTGTGATAACCCCTGAAATAGATAACATACTTCCCGGAATTACCAGAAGCAAGG
TCATCAAACTCCTCAAAGAAAGTGGAATAGAAGTGGTGGAAAGAAGAGTATATCTGGAGGAATTCCTTAGTGCTGATGAA
GTCTTCCTGGTAAGTACCGTTTCCATGGTGATGCCAGTGCGAAGAATAGACGACAGAGAATTCAAGGCTCCCGGTGAAAT
TACCTTAAAAGCTATGGAACTTTACAGAGACTTTATGGACAAAGAAAAAGAGCGGTATTAA

Upstream 100 bases:

>100_bases
CGGTAAATGTAGCCAGGGGGGACGGGAAAAGCGCGGTAGCTGCCAGGAGGGCTATGGAAAGAGCAGGCTGGTTTAATCAG
AAAGGAAGTGGTCAGAATCA

Downstream 100 bases:

>100_bases
CCGCCCCTATTTTTTGAACCTATCTCTTAACTGGTTGATGTATGTCACCATCACGATTTCGTTTTTCACTGTTACATCTA
TAAGCGTGAAAATCCCCGTA

Product: aminotransferase class IV

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKAFLNGKFVEKNEKLITLENRGLTFGDGLFEVLRVKDGHIFFFEDHLLRMKSSASFFGIPFPYSLDEIKNRAHELISLN
RINDGELYIELTRGVDTNREHRYPPRSTQTTFFMLAIPLREINPENWKRGAAVFSFPDLRHKLCEHKTINLLPNVMAKNF
AYERGGYEALMYRENSKGKYITEGGSSNYFLVKNCTVITPEIDNILPGITRSKVIKLLKESGIEVVERRVYLEEFLSADE
VFLVSTVSMVMPVRRIDDREFKAPGEITLKAMELYRDFMDKEKERY

Sequences:

>Translated_286_residues
MKAFLNGKFVEKNEKLITLENRGLTFGDGLFEVLRVKDGHIFFFEDHLLRMKSSASFFGIPFPYSLDEIKNRAHELISLN
RINDGELYIELTRGVDTNREHRYPPRSTQTTFFMLAIPLREINPENWKRGAAVFSFPDLRHKLCEHKTINLLPNVMAKNF
AYERGGYEALMYRENSKGKYITEGGSSNYFLVKNCTVITPEIDNILPGITRSKVIKLLKESGIEVVERRVYLEEFLSADE
VFLVSTVSMVMPVRRIDDREFKAPGEITLKAMELYRDFMDKEKERY
>Mature_286_residues
MKAFLNGKFVEKNEKLITLENRGLTFGDGLFEVLRVKDGHIFFFEDHLLRMKSSASFFGIPFPYSLDEIKNRAHELISLN
RINDGELYIELTRGVDTNREHRYPPRSTQTTFFMLAIPLREINPENWKRGAAVFSFPDLRHKLCEHKTINLLPNVMAKNF
AYERGGYEALMYRENSKGKYITEGGSSNYFLVKNCTVITPEIDNILPGITRSKVIKLLKESGIEVVERRVYLEEFLSADE
VFLVSTVSMVMPVRRIDDREFKAPGEITLKAMELYRDFMDKEKERY

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=271, Percent_Identity=28.7822878228782, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787338, Length=268, Percent_Identity=26.4925373134328, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 33302; Mature: 33302

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFLNGKFVEKNEKLITLENRGLTFGDGLFEVLRVKDGHIFFFEDHLLRMKSSASFFGI
CCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHEECCCEEEEEEHHHHHHHCCCCEECC
PFPYSLDEIKNRAHELISLNRINDGELYIELTRGVDTNREHRYPPRSTQTTFFMLAIPLR
CCCCCHHHHHHHHHHHEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEHH
EINPENWKRGAAVFSFPDLRHKLCEHKTINLLPNVMAKNFAYERGGYEALMYRENSKGKY
HCCCCHHHCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
ITEGGSSNYFLVKNCTVITPEIDNILPGITRSKVIKLLKESGIEVVERRVYLEEFLSADE
EECCCCCCEEEEECCEEECCCHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
VFLVSTVSMVMPVRRIDDREFKAPGEITLKAMELYRDFMDKEKERY
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAFLNGKFVEKNEKLITLENRGLTFGDGLFEVLRVKDGHIFFFEDHLLRMKSSASFFGI
CCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHEECCCEEEEEEHHHHHHHCCCCEECC
PFPYSLDEIKNRAHELISLNRINDGELYIELTRGVDTNREHRYPPRSTQTTFFMLAIPLR
CCCCCHHHHHHHHHHHEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEHH
EINPENWKRGAAVFSFPDLRHKLCEHKTINLLPNVMAKNFAYERGGYEALMYRENSKGKY
HCCCCHHHCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
ITEGGSSNYFLVKNCTVITPEIDNILPGITRSKVIKLLKESGIEVVERRVYLEEFLSADE
EECCCCCCEEEEECCEEECCCHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
VFLVSTVSMVMPVRRIDDREFKAPGEITLKAMELYRDFMDKEKERY
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]