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The map label for this gene is trmFO [H]

Identifier: 239616442

GI number: 239616442

Start: 38770

End: 40083

Strand: Reverse

Name: trmFO [H]

Synonym: Kole_0031

Alternate gene names: 239616442

Gene position: 40083-38770 (Counterclockwise)

Preceding gene: 239616443

Following gene: 239616441

Centisome position: 1.74

GC content: 45.89

Gene sequence:

>1314_bases
GTGAAGGTGAAGATAAATATAGTCGGTGGTGGGCTGGCCGGGTCTGAAGCGGCCCTGAGCCTAGCTGAAAAGGGAATAGA
AGTAACATTATACGAGATGAGACCAAAGGTAAAAACCGATGTTCACCAGACGGGGTATTTTGCTGAACTGGTGTGCAGTA
ACTCTCTTAAATCCGTGGAATTAACCAACGCTTCAGGATTGTTGAAAGAGGAAGCCAGGATGCTGGGTTCAATTCTTCTG
GAGATAGCGGAAAGACACCGCGTTCCTGCCGGAAAAGCTTTCGCGGTTGATAGAAAAACCTTCTCTAAGGAGGTTACCGA
AAGGATTGAAAGCTCCCCATATATTCATGTGGTGAGAGAAGAAGTCAAAGAAATTGATCTCAACGATGAGGGAACGCTCT
GGATTATCGCTACCGGTCCTCTTTCTTCGCCGGCTTTTGAGAAATGGCTTTCGGGTTTGTTTGGAGAGGACCTTTTCTTT
TTCGATGCTCTGTCACCAATAGTAGCAGCGGATTCTATTGATTATAGACGCTGTTTTGTCGGCGATCGTTACGGGAAGGG
AAGCGGCGATTATCTGAACTGTCCCCTTGATAAGGAAACCTACGAGAGATTCTGGAATGAACTTGTGAATGCCCAGGTTG
CTCCAATAGAGAATTTTTCCGACAAGCTTCTCTTTGATCGCTGTCAACCGGTTGAGGAGATCGCGAGAACAGGCATCGAT
GCTTTGCGCTATGGTCCAATGAAACCTGTAGGATTGATAGATCCGAAAACGGGTAGTGAACCCTACGCGGTGGTTCAGCT
AAGAAAAGAAAATAAAGAGGGGACCCTTTACAACCTTGTCGGGTTTCAAACCCGTCTTAAATGGGGAGAGCAAAAACGGG
TATTTCGGATAATTCCTGCTTTAGAGCATGCTGAATTTGTTCGGTATGGTGTTATGCACCGAAATACCTATCTGAATTCC
CCGAAGATTCTGAATAGAGATCTAAGAAGCAAGGGTTATCCAAACCTGTTTTTCTGTGGTCAAATAACAGGATTGGAAGG
TTATGTTGAGGCCATCACAAGCGGGAGATTTGTCGCATTGAACGTCATGGAGTTAGTGAAAACCGGTTCAACCTTTTACC
TGCCGGAAAAGACCATGCTTGGAGCCTTGATAAACCACATAACGGTCTCCGGGAGGGTTCCCCTTTCTCCCGTGTACGCA
AATTATGGTCTCCTTCCCCCCGTTAAAGCGAAGGGGAAGAGAGAAAGGAACCTGAAAAAGGCCGAGAGAGCTATTGAGGA
GTTAAAAAAGTTTCTTAAAGAAAGGGGCTTTTGA

Upstream 100 bases:

>100_bases
TAATGGAGTTTGGCATGGAAACCTTTGCCCCTTACGCAGGTTACTATCAGGAAGCTGTGTTCAGATACTATAGAACGCAC
AAATTGGGGAGAGATAAACA

Downstream 100 bases:

>100_bases
GATGATATACCTTTCTGAACCCCTGTTTTCTGAACGCCCGTGGGGGGATCCTGAACTCAACAAGCTGTATGGTGTGAATT
CGGAGCTTCCCATCGGTGAG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKVKINIVGGGLAGSEAALSLAEKGIEVTLYEMRPKVKTDVHQTGYFAELVCSNSLKSVELTNASGLLKEEARMLGSILL
EIAERHRVPAGKAFAVDRKTFSKEVTERIESSPYIHVVREEVKEIDLNDEGTLWIIATGPLSSPAFEKWLSGLFGEDLFF
FDALSPIVAADSIDYRRCFVGDRYGKGSGDYLNCPLDKETYERFWNELVNAQVAPIENFSDKLLFDRCQPVEEIARTGID
ALRYGPMKPVGLIDPKTGSEPYAVVQLRKENKEGTLYNLVGFQTRLKWGEQKRVFRIIPALEHAEFVRYGVMHRNTYLNS
PKILNRDLRSKGYPNLFFCGQITGLEGYVEAITSGRFVALNVMELVKTGSTFYLPEKTMLGALINHITVSGRVPLSPVYA
NYGLLPPVKAKGKRERNLKKAERAIEELKKFLKERGF

Sequences:

>Translated_437_residues
MKVKINIVGGGLAGSEAALSLAEKGIEVTLYEMRPKVKTDVHQTGYFAELVCSNSLKSVELTNASGLLKEEARMLGSILL
EIAERHRVPAGKAFAVDRKTFSKEVTERIESSPYIHVVREEVKEIDLNDEGTLWIIATGPLSSPAFEKWLSGLFGEDLFF
FDALSPIVAADSIDYRRCFVGDRYGKGSGDYLNCPLDKETYERFWNELVNAQVAPIENFSDKLLFDRCQPVEEIARTGID
ALRYGPMKPVGLIDPKTGSEPYAVVQLRKENKEGTLYNLVGFQTRLKWGEQKRVFRIIPALEHAEFVRYGVMHRNTYLNS
PKILNRDLRSKGYPNLFFCGQITGLEGYVEAITSGRFVALNVMELVKTGSTFYLPEKTMLGALINHITVSGRVPLSPVYA
NYGLLPPVKAKGKRERNLKKAERAIEELKKFLKERGF
>Mature_437_residues
MKVKINIVGGGLAGSEAALSLAEKGIEVTLYEMRPKVKTDVHQTGYFAELVCSNSLKSVELTNASGLLKEEARMLGSILL
EIAERHRVPAGKAFAVDRKTFSKEVTERIESSPYIHVVREEVKEIDLNDEGTLWIIATGPLSSPAFEKWLSGLFGEDLFF
FDALSPIVAADSIDYRRCFVGDRYGKGSGDYLNCPLDKETYERFWNELVNAQVAPIENFSDKLLFDRCQPVEEIARTGID
ALRYGPMKPVGLIDPKTGSEPYAVVQLRKENKEGTLYNLVGFQTRLKWGEQKRVFRIIPALEHAEFVRYGVMHRNTYLNS
PKILNRDLRSKGYPNLFFCGQITGLEGYVEAITSGRFVALNVMELVKTGSTFYLPEKTMLGALINHITVSGRVPLSPVYA
NYGLLPPVKAKGKRERNLKKAERAIEELKKFLKERGF

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49088; Mature: 49088

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKINIVGGGLAGSEAALSLAEKGIEVTLYEMRPKVKTDVHQTGYFAELVCSNSLKSVE
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCHHHHHHHCCCCCEEE
LTNASGLLKEEARMLGSILLEIAERHRVPAGKAFAVDRKTFSKEVTERIESSPYIHVVRE
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCEEHHHHH
EVKEIDLNDEGTLWIIATGPLSSPAFEKWLSGLFGEDLFFFDALSPIVAADSIDYRRCFV
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHCC
GDRYGKGSGDYLNCPLDKETYERFWNELVNAQVAPIENFSDKLLFDRCQPVEEIARTGID
CCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHCCHH
ALRYGPMKPVGLIDPKTGSEPYAVVQLRKENKEGTLYNLVGFQTRLKWGEQKRVFRIIPA
HHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEHHHHHHHCCCCCCHHHHHHHH
LEHAEFVRYGVMHRNTYLNSPKILNRDLRSKGYPNLFFCGQITGLEGYVEAITSGRFVAL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCEEEE
NVMELVKTGSTFYLPEKTMLGALINHITVSGRVPLSPVYANYGLLPPVKAKGKRERNLKK
EHHHHHHCCCEEECCHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHH
AERAIEELKKFLKERGF
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVKINIVGGGLAGSEAALSLAEKGIEVTLYEMRPKVKTDVHQTGYFAELVCSNSLKSVE
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCHHHHHHHCCCCCEEE
LTNASGLLKEEARMLGSILLEIAERHRVPAGKAFAVDRKTFSKEVTERIESSPYIHVVRE
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCEEHHHHH
EVKEIDLNDEGTLWIIATGPLSSPAFEKWLSGLFGEDLFFFDALSPIVAADSIDYRRCFV
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHCC
GDRYGKGSGDYLNCPLDKETYERFWNELVNAQVAPIENFSDKLLFDRCQPVEEIARTGID
CCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHCCHH
ALRYGPMKPVGLIDPKTGSEPYAVVQLRKENKEGTLYNLVGFQTRLKWGEQKRVFRIIPA
HHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEHHHHHHHCCCCCCHHHHHHHH
LEHAEFVRYGVMHRNTYLNSPKILNRDLRSKGYPNLFFCGQITGLEGYVEAITSGRFVAL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCEEEE
NVMELVKTGSTFYLPEKTMLGALINHITVSGRVPLSPVYANYGLLPPVKAKGKRERNLKK
EHHHHHHCCCEEECCHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHH
AERAIEELKKFLKERGF
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA