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The map label for this gene is guaB [C]

Identifier: 239616437

GI number: 239616437

Start: 34766

End: 35722

Strand: Reverse

Name: guaB [C]

Synonym: Kole_0026

Alternate gene names: 239616437

Gene position: 35722-34766 (Counterclockwise)

Preceding gene: 239616438

Following gene: 239616436

Centisome position: 1.55

GC content: 42.11

Gene sequence:

>957_bases
TTGAGCGACGAAGGCGTTGATAAACTAATTGAAAAACTCAGAAAGTTTTTTTCGCATATCAAAGCTGGCGAAATAATGAC
CAAGAACATAATCACAATGACTCCAGAAAGAACCCTCTGGCAAGCCAAAGAATTGATGAAGCTCCGAAAAATCTCCGGCA
TCCCTATTGTAAATCGCGACAATAAGCTCCTGGGAATCGTGAGTATTGAGGATATCATTGTTGCTCTTGAGAAGGATCAC
ATCAGGGACAAAATAGGGGAGCACATGACAAAAGACGTGATCGTTCTCAAACCAGATGAAGAACTTGAATCGATACTGCA
AAAATTCGACAGGTATCGTTATGGGCGTTTTCCCGTTGTCGATGAATCCGGGAAACTCGTGGGTTTAGTAACCAAAAAGG
ATATAATCAGCGCGATCCTTGAACGATTCAGGATCATATACGTTCATGACGAGCGTAGAAGCAGAGTATTGGAAAATACT
AATAAATGGTTTGAACGCTCGTTAATTACAGGCGATTACGTCGAAAAAAAGAAAGCGGATTTCTTCTTTGCGATAAATTA
CACCGACGTGGACCTTGCAGGAATGGGAGCTGCAAGATTGAAACGCTTCTTAATGGGAAAGATAAAAGATGAGAATCTTG
TGCGCAAGGTATCGATTGCCACCTATGAGGCTGAGGTCAATGTGGTCATTCACAGTGGAAGCGACGGCTTCATCTACTGC
TGGATAGACGATGATGCCATCAGGGTGCGAGTTGAAGATCACGGCAAAGGTATTGAAAATATTGAAATGGCCATGAAGGA
AGGATATTCCACCGCCACTGATCATGTCAGGGAATTAGGATTTGGAGCTGGAATGGGACTCCCAAATATGAAGCGCTATT
CTGATAAGATGGTGGTCATGTCAGAAGTGGGAAAAGGAGTCGTTGTAGAAATGATATTTTACATGAACAAAAAATAA

Upstream 100 bases:

>100_bases
TGGAAATCCCTCTATTGCACGCACGGATTTCAATGTACAAGGCATGTGGAAAACTCTATGACCTCAACCTCAAAGATGCG
TTAGAGGAAGTGAATGGGTT

Downstream 100 bases:

>100_bases
TCGCTATTATCGGTTTGATTTAGAGGGGAGGGGTCTTCTTTGCTTTTGAGAGAGATTATTGATAAATGCAATCTTAAAGT
GATTGAAGCCGAAAACCTTG

Product: signal transduction protein with CBS domains

Products: H+; Nicotinamide adenine dinucleotide - reduced; Xanthosine 5'-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSDEGVDKLIEKLRKFFSHIKAGEIMTKNIITMTPERTLWQAKELMKLRKISGIPIVNRDNKLLGIVSIEDIIVALEKDH
IRDKIGEHMTKDVIVLKPDEELESILQKFDRYRYGRFPVVDESGKLVGLVTKKDIISAILERFRIIYVHDERRSRVLENT
NKWFERSLITGDYVEKKKADFFFAINYTDVDLAGMGAARLKRFLMGKIKDENLVRKVSIATYEAEVNVVIHSGSDGFIYC
WIDDDAIRVRVEDHGKGIENIEMAMKEGYSTATDHVRELGFGAGMGLPNMKRYSDKMVVMSEVGKGVVVEMIFYMNKK

Sequences:

>Translated_318_residues
MSDEGVDKLIEKLRKFFSHIKAGEIMTKNIITMTPERTLWQAKELMKLRKISGIPIVNRDNKLLGIVSIEDIIVALEKDH
IRDKIGEHMTKDVIVLKPDEELESILQKFDRYRYGRFPVVDESGKLVGLVTKKDIISAILERFRIIYVHDERRSRVLENT
NKWFERSLITGDYVEKKKADFFFAINYTDVDLAGMGAARLKRFLMGKIKDENLVRKVSIATYEAEVNVVIHSGSDGFIYC
WIDDDAIRVRVEDHGKGIENIEMAMKEGYSTATDHVRELGFGAGMGLPNMKRYSDKMVVMSEVGKGVVVEMIFYMNKK
>Mature_317_residues
SDEGVDKLIEKLRKFFSHIKAGEIMTKNIITMTPERTLWQAKELMKLRKISGIPIVNRDNKLLGIVSIEDIIVALEKDHI
RDKIGEHMTKDVIVLKPDEELESILQKFDRYRYGRFPVVDESGKLVGLVTKKDIISAILERFRIIYVHDERRSRVLENTN
KWFERSLITGDYVEKKKADFFFAINYTDVDLAGMGAARLKRFLMGKIKDENLVRKVSIATYEAEVNVVIHSGSDGFIYCW
IDDDAIRVRVEDHGKGIENIEMAMKEGYSTATDHVRELGFGAGMGLPNMKRYSDKMVVMSEVGKGVVVEMIFYMNKK

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains [H]

Homologues:

None

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001807
- InterPro:   IPR000644
- InterPro:   IPR016436
- InterPro:   IPR005104
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00571 CBS; PF03444 DUF293 [H]

EC number: 1.1.1.205 [C]

Molecular weight: Translated: 36564; Mature: 36433

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEGVDKLIEKLRKFFSHIKAGEIMTKNIITMTPERTLWQAKELMKLRKISGIPIVNRD
CCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHCCCEEECCC
NKLLGIVSIEDIIVALEKDHIRDKIGEHMTKDVIVLKPDEELESILQKFDRYRYGRFPVV
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCEE
DESGKLVGLVTKKDIISAILERFRIIYVHDERRSRVLENTNKWFERSLITGDYVEKKKAD
CCCCCEEEEEEHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
FFFAINYTDVDLAGMGAARLKRFLMGKIKDENLVRKVSIATYEAEVNVVIHSGSDGFIYC
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHEEEEEEEEEEEEEEEEECCCCCEEEE
WIDDDAIRVRVEDHGKGIENIEMAMKEGYSTATDHVRELGFGAGMGLPNMKRYSDKMVVM
EECCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCEEEE
SEVGKGVVVEMIFYMNKK
HHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SDEGVDKLIEKLRKFFSHIKAGEIMTKNIITMTPERTLWQAKELMKLRKISGIPIVNRD
CCCHHHHHHHHHHHHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHCCCEEECCC
NKLLGIVSIEDIIVALEKDHIRDKIGEHMTKDVIVLKPDEELESILQKFDRYRYGRFPVV
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCEE
DESGKLVGLVTKKDIISAILERFRIIYVHDERRSRVLENTNKWFERSLITGDYVEKKKAD
CCCCCEEEEEEHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
FFFAINYTDVDLAGMGAARLKRFLMGKIKDENLVRKVSIATYEAEVNVVIHSGSDGFIYC
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHEEEEEEEEEEEEEEEEECCCCCEEEE
WIDDDAIRVRVEDHGKGIENIEMAMKEGYSTATDHVRELGFGAGMGLPNMKRYSDKMVVM
EECCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCEEEE
SEVGKGVVVEMIFYMNKK
HHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; IMP; Nicotinamide adenine dinucleotide [C]

Specific reaction: H2O + IMP + Nicotinamide adenine dinucleotide --> H+ + Nicotinamide adenine dinucleotide - reduced + Xanthosine 5'-phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]